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P10613

- CP51_CANAL

UniProt

P10613 - CP51_CANAL

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Protein

Lanosterol 14-alpha demethylase

Gene

ERG11

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.

Catalytic activityi

A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.

Cofactori

hemeBy similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi470 – 4701Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. alkane 1-monooxygenase activity Source: CGD
  2. C-22 sterol desaturase activity Source: CGD
  3. drug binding Source: CGD
  4. heme binding Source: InterPro
  5. iron ion binding Source: InterPro
  6. oxidoreductase activity Source: CGD
  7. oxygen binding Source: CGD
  8. sterol 14-demethylase activity Source: CGD

GO - Biological processi

  1. cell growth mode switching, budding to filamentous Source: CGD
  2. cellular response to drug Source: CGD
  3. cellular response to starvation Source: CGD
  4. chlamydospore formation Source: CGD
  5. demethylation Source: GOC
  6. ergosterol biosynthetic process Source: CGD
  7. fatty acid omega-oxidation Source: CGD
  8. filamentous growth Source: CGD
  9. filamentous growth of a population of unicellular organisms in response to biotic stimulus Source: CGD
  10. filamentous growth of a population of unicellular organisms in response to starvation Source: CGD
  11. lauric acid metabolic process Source: CGD
  12. membrane raft polarization Source: CGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.14.13.70. 1096.
UniPathwayiUPA00770; UER00754.

Names & Taxonomyi

Protein namesi
Recommended name:
Lanosterol 14-alpha demethylase (EC:1.14.13.70)
Alternative name(s):
CYPLI
Cytochrome P450 51
Cytochrome P450-14DM
Cytochrome P450-LIA1
Sterol 14-alpha demethylase
Gene namesi
Name:ERG11
Synonyms:CYP51, ERG16
ORF Names:CaO19.922
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
ProteomesiUP000000559: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0003627. ERG11.

Subcellular locationi

Membrane Curated

GO - Cellular componenti

  1. endoplasmic reticulum Source: CGD
  2. integral component of membrane Source: CGD
  3. membrane Source: CGD
  4. plasma membrane Source: CGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 528528Lanosterol 14-alpha demethylasePRO_0000052003Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi5476.CAL0003627.

Structurei

3D structure databases

ProteinModelPortaliP10613.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
InParanoidiP10613.
KOiK05917.
OrthoDBiEOG7712HB.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10613-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAIVETVIDG INYFLSLSVT QQISILLGVP FVYNLVWQYL YSLRKDRAPL
60 70 80 90 100
VFYWIPWFGS AASYGQQPYE FFESCRQKYG DVFSFMLLGK IMTVYLGPKG
110 120 130 140 150
HEFVFNAKLS DVSAEDAYKH LTTPVFGKGV IYDCPNSRLM EQKKFAKFAL
160 170 180 190 200
TTDSFKRYVP KIREEILNYF VTDESFKLKE KTHGVANVMK TQPEITIFTA
210 220 230 240 250
SRSLFGDEMR RIFDRSFAQL YSDLDKGFTP INFVFPNLPL PHYWRRDAAQ
260 270 280 290 300
KKISATYMKE IKSRRERGDI DPNRDLIDSL LIHSTYKDGV KMTDQEIANL
310 320 330 340 350
LIGILMGGQH TSASTSAWFL LHLGEKPHLQ DVIYQEVVEL LKEKGGDLND
360 370 380 390 400
LTYEDLQKLP SVNNTIKETL RMHMPLHSIF RKVTNPLRIP ETNYIVPKGH
410 420 430 440 450
YVLVSPGYAH TSERYFDNPE DFDPTRWDTA AAKANSVSFN SSDEVDYGFG
460 470 480 490 500
KVSKGVSSPY LPFGGGRHRC IGEQFAYVQL GTILTTFVYN LRWTIDGYKV
510 520
PDPDYSSMVV LPTEPAEIIW EKRETCMF
Length:528
Mass (Da):60,675
Last modified:November 1, 1995 - v2
Checksum:i026948CEE4AF1B88
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 191V → D in AAB08099. (PubMed:9210671)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti105 – 1051F → L in fluconazole-resistant isolates.
Natural varianti266 – 2661E → D in strain: fluconazole-resistant isolates.
Natural varianti287 – 2871K → R in strain: fluconazole-resistant isolates.
Natural varianti450 – 4501G → E in strain: fluconazole-resistant isolates.
Natural varianti464 – 4641G → S in strain: fluconazole-resistant isolates.
Natural varianti467 – 4671R → K in strain: Isolate 13; azole-resistant. 1 Publication
Natural varianti488 – 4881V → I in strain: fluconazole-resistant isolates.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13296 Genomic DNA. Translation: CAA31658.1.
EU819548 Genomic DNA. Translation: ACF34636.1.
EU819551 Genomic DNA. Translation: ACF34639.1.
FJ002303 Genomic DNA. Translation: ACH91036.1.
FJ403378 Genomic DNA. Translation: ACJ23064.1.
AACQ01000063 Genomic DNA. Translation: EAK97774.1.
U67192 Genomic DNA. Translation: AAB08099.1.
U67193 Genomic DNA. Translation: AAB08100.1.
PIRiS02713. O4CK51.
RefSeqiXP_716761.1. XM_711668.1.

Genome annotation databases

GeneIDi3641571.
KEGGical:CaO19.922.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13296 Genomic DNA. Translation: CAA31658.1 .
EU819548 Genomic DNA. Translation: ACF34636.1 .
EU819551 Genomic DNA. Translation: ACF34639.1 .
FJ002303 Genomic DNA. Translation: ACH91036.1 .
FJ403378 Genomic DNA. Translation: ACJ23064.1 .
AACQ01000063 Genomic DNA. Translation: EAK97774.1 .
U67192 Genomic DNA. Translation: AAB08099.1 .
U67193 Genomic DNA. Translation: AAB08100.1 .
PIRi S02713. O4CK51.
RefSeqi XP_716761.1. XM_711668.1.

3D structure databases

ProteinModelPortali P10613.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5476.CAL0003627.

Chemistry

ChEMBLi CHEMBL1780.
DrugBanki DB04794. Bifonazole.
DB00257. Clotrimazole.
DB01127. Econazole.
DB00196. Fluconazole.
DB01026. Ketoconazole.
DB08933. LULICONAZOLE.
DB01110. Miconazole.
DB00239. Oxiconazole.
DB01263. Posaconazole.
DB01153. Sertaconazole.
DB00251. Terconazole.
DB01007. Tioconazole.
DB00582. Voriconazole.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3641571.
KEGGi cal:CaO19.922.

Organism-specific databases

CGDi CAL0003627. ERG11.

Phylogenomic databases

eggNOGi COG2124.
InParanoidi P10613.
KOi K05917.
OrthoDBi EOG7712HB.

Enzyme and pathway databases

UniPathwayi UPA00770 ; UER00754 .
BRENDAi 1.14.13.70. 1096.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00465. EP450IV.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of cytochrome P450 L1A1 (lanosterol 14 alpha-demethylase) from Candida albicans."
    Lai M.H., Kirsch D.R.
    Nucleic Acids Res. 17:804-804(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SC5314 / ATCC MYA-2876.
  2. "ERG11 mutations in oral strains of Candida albicans isolated in Sardinia."
    Orru G., Ciusa M.L., Pilia F., Taccori F., Cosentino S., Pisano M.B., Meloni M., Fadda M.E.
    Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CA100, CA11, CA36 and CA95.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SC5314 / ATCC MYA-2876.
  4. "The presence of an R467K amino acid substitution and loss of allelic variation correlate with an azole-resistant lanosterol 14-alpha demethylase in Candida albicans."
    White T.C.
    Antimicrob. Agents Chemother. 41:1488-1494(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-48 AND 467-528, VARIANT LYS-467.
    Strain: SS.
  5. "Modeling cytochrome P450 14-alpha demethylase (Candida albicans) from P450cam."
    Boscott P.E., Grant G.H.
    J. Mol. Graph. 12:185-192(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING.
  6. "Molecular analysis of cyp51 from fluconazole-resistant Candida albicans strains."
    Loffler J., Kelly S.L., Hebart H., Schumacher U., Lass-Florl C., Einsele H.
    FEMS Microbiol. Lett. 151:263-268(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS FLUCONAZOLE-RESISTANT.

Entry informationi

Entry nameiCP51_CANAL
AccessioniPrimary (citable) accession number: P10613
Secondary accession number(s): Q5A524, Q92208
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1995
Last modified: November 26, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3