Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P10612

- CP11A_PIG

UniProt

P10612 - CP11A_PIG

Protein

Cholesterol side-chain cleavage enzyme, mitochondrial

Gene

CYP11A1

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.

    Catalytic activityi

    Cholesterol + 6 reduced adrenodoxin + 3 O2 = pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O.

    Cofactori

    Heme group.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi461 – 4611Iron (heme axial ligand)

    GO - Molecular functioni

    1. cholesterol monooxygenase (side-chain-cleaving) activity Source: UniProtKB
    2. heme binding Source: UniProtKB
    3. iron ion binding Source: InterPro

    GO - Biological processi

    1. C21-steroid hormone biosynthetic process Source: UniProtKB
    2. cholesterol metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Steroidogenesis, Sterol metabolism

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_205037. Pregnenolone biosynthesis.
    REACT_211636. Endogenous sterols.
    UniPathwayiUPA00229.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cholesterol side-chain cleavage enzyme, mitochondrial (EC:1.14.15.6)
    Alternative name(s):
    CYPXIA1
    Cholesterol desmolase
    Cytochrome P450 11A1
    Cytochrome P450(scc)
    Gene namesi
    Name:CYP11A1
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3939Mitochondrion1 PublicationAdd
    BLAST
    Chaini40 – 520481Cholesterol side-chain cleavage enzyme, mitochondrialPRO_0000003588Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with FDX1/adrenodoxin.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP10612.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOVERGENiHBG051098.
    KOiK00498.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P10612-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLARGLALRS VLVKGCQPFL SAPRECPGHP RVGTGEGACI STKTPRPFSE    50
    IPSPGDNGWI NLYRFWKEKG TQKIHYHHVQ NFQKYGPIYR EKLGNLESVY 100
    IIDPEDVALL FKFEGPNPER YNIPPWVAYH QHYQKPVGVL LKKSGAWKKD 150
    RLVLNTEVMA PEAIKNFIPL LDTVSQDFVG VLHRRIKQQG SGKFSGDIRE 200
    DLFRFAFESI TNVIFGERLG MLEEIVDPEA QKFIDAVYQM FHTSVPMLNL 250
    PPDLFRLFRT KTWRDHVAAW DTIFNKAEKY TQNFYWDLRR KREFNNYPGI 300
    LYRLLGNDKL LSEDVKANVT EMLAGGVDTT SMTLQWHLYE MARSLNVQEM 350
    LREEVLNARR QAQGDTSKML QLVPLLKASI KETLRLHPIS VTLQRYLVND 400
    LVLRDYMIPA KTLVQVAVYA MGRDPAFFSN PGQFDPTRWL GKERDLIHFR 450
    NLGFGWGVRQ CVGRRIAELE MTLFLIHILE NFKVELQHFS DVDTIFNLIL 500
    MPDKPIFLVF RPFNQDPLQA 520
    Length:520
    Mass (Da):60,258
    Last modified:July 1, 1989 - v1
    Checksum:i6C6D0D6FE34F9C33
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti46 – 461R → F AA sequence (PubMed:1773895)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13768 mRNA. Translation: CAA32018.1.
    PIRiS03188.
    RefSeqiNP_999592.1. NM_214427.1.
    UniGeneiSsc.8776.

    Genome annotation databases

    GeneIDi403329.
    KEGGissc:403329.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13768 mRNA. Translation: CAA32018.1 .
    PIRi S03188.
    RefSeqi NP_999592.1. NM_214427.1.
    UniGenei Ssc.8776.

    3D structure databases

    ProteinModelPortali P10612.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 403329.
    KEGGi ssc:403329.

    Organism-specific databases

    CTDi 1583.

    Phylogenomic databases

    HOVERGENi HBG051098.
    KOi K00498.

    Enzyme and pathway databases

    UniPathwayi UPA00229 .
    Reactomei REACT_205037. Pregnenolone biosynthesis.
    REACT_211636. Endogenous sterols.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of cytochrome P-450 cholesterol side-chain cleavage cDNA isolated from porcine testis."
      Mulheron G.W., Stone R.T., Miller W.L., Wise T.H.
      Nucleic Acids Res. 17:1773-1773(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Testis.
    2. "Purification and properties of cytochrome P-450 (SCC) from pig testis mitochondria."
      Kuwada M., Kitajima R., Suzuki H., Horie S.
      Biochem. Biophys. Res. Commun. 176:1501-1508(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 41-61.
      Tissue: Testis.
    3. "Purification and comparative characterization of cytochrome P-450scc from porcine adrenocortical mitochondria."
      Iwahashi K., Tsubaki M., Miyatake A., Ichikawa Y.
      Int. J. Biochem. 23:901-909(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 40-64.

    Entry informationi

    Entry nameiCP11A_PIG
    AccessioniPrimary (citable) accession number: P10612
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3