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P10611

- CP4A4_RABIT

UniProt

P10611 - CP4A4_RABIT

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Protein

Cytochrome P450 4A4

Gene
CYP4A4
Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Catalyzes the omega- and (omega-1)-hydroxylation of fatty acids.1 Publication

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

Heme group By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei321 – 3211Heme (covalent; via 1 link) By similarity
Metal bindingi457 – 4571Iron (heme axial ligand)

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 4A4 (EC:1.14.14.1)
Alternative name(s):
CYPIVA4
Cytochrome P450-P-2
Prostaglandin omega-hydroxylase
Gene namesi
Name:CYP4A4
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei11 – 3121Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 44 InferredPRO_0000003571
Chaini5 – 510506Cytochrome P450 4A4PRO_0000003572Add
BLAST

Expressioni

Inductioni

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000001485.

Structurei

3D structure databases

ProteinModelPortaliP10611.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10611-1 [UniParc]FASTAAdd to Basket

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MSVSALSPTR LPGSLSGLLQ VAALLGLLLL LLKAAQLYLH RQWLLRALQQ    50
FPCPPFHWLL GHSREFQNDQ ELERIQKWVE KFPGACPWWL SGNKARLLVY 100
DPDYLKVILG RSDPKAPRNY KLMTPWIGYG LLLLDGQTWF QHRRMLTPAF 150
HYDILKPYVG LMVDSVQIML DRWEQLISQD SSLEIFQHVS LMTLDTIMKC 200
AFSYQGSVQL DRNSHSYIQA INDLNNLVFY RARNVFHQSD FLYRLSPEGR 250
LFHRACQLAH EHTDRVIQQR KAQLQQEGEL EKVRRKRRLD FLDVLLFAKM 300
ENGSSLSDQD LRAEVDTFMF EGHDTTASGV SWIFYALATH PEHQHRCREE 350
IQGLLGDGAS ITWEHLDQMP YTTMCIKEAL RLYPPVPSVT RQLSKPVTFP 400
DGRSLPKGVI LFLSIYGLHY NPKVWQNPEV FDPFRFAPDS AYHSHAFLPF 450
SGGARNCIGK QFAMRELKVA VALTLVRFEL LPDPTRIPIP IARVVLKSKN 500
GIHLRLRKLH 510
Length:510
Mass (Da):58,930
Last modified:July 19, 2004 - v3
Checksum:i50CB1DE9D9D7FD5D
GO

Sequence cautioni

The sequence AAA31228.1 differs from that shown. Reason: Frameshift at position 23.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L04758 Genomic DNA. Translation: AAA31228.1. Frameshift.
J02818 mRNA. Translation: AAA31232.1.
PIRiS32315. A29368.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L04758 Genomic DNA. Translation: AAA31228.1 . Frameshift.
J02818 mRNA. Translation: AAA31232.1 .
PIRi S32315. A29368.

3D structure databases

ProteinModelPortali P10611.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9986.ENSOCUP00000001485.

Chemistry

BindingDBi P10611.
ChEMBLi CHEMBL4743.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000233833.
HOVERGENi HBG000182.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Rabbit prostaglandin omega-hydroxylase (CYP4A4): gene structure and expression."
    Palmer C.N., Griffin K.J., Johnson E.F.
    Arch. Biochem. Biophys. 300:670-676(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-65.
    Strain: III/J.
  2. "cDNA cloning and inducible expression during pregnancy of the mRNA for rabbit pulmonary prostaglandin omega-hydroxylase (cytochrome P-450p-2)."
    Matsubara S., Yamamoto S., Sogawa K., Yokotani N., Fujii-Kuriyama Y., Haniu M., Shively J.E., Gotoh O., Kusunose E., Kusunose M.
    J. Biol. Chem. 262:13366-13371(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 21-510, PROTEIN SEQUENCE OF 5-29.
  3. "Purification and characterization of two forms of fatty acid omega-hydroxylase cytochrome P-450 from rabbit kidney cortex microsomes."
    Yoshimura R., Kusunose E., Yokotani N., Yamamoto S., Kubota I., Kusunose M.
    J. Biochem. 108:544-548(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiCP4A4_RABIT
AccessioniPrimary (citable) accession number: P10611
Secondary accession number(s): Q02927
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 19, 2004
Last modified: February 19, 2014
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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