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P10611

- CP4A4_RABIT

UniProt

P10611 - CP4A4_RABIT

Protein

Cytochrome P450 4A4

Gene

CYP4A4

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 3 (19 Jul 2004)
      Previous versions | rss
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    Functioni

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Catalyzes the omega- and (omega-1)-hydroxylation of fatty acids.1 Publication

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei321 – 3211Heme (covalent; via 1 link)By similarity
    Metal bindingi457 – 4571Iron (heme axial ligand)

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 4A4 (EC:1.14.14.1)
    Alternative name(s):
    CYPIVA4
    Cytochrome P450-P-2
    Prostaglandin omega-hydroxylase
    Gene namesi
    Name:CYP4A4
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 441 PublicationPRO_0000003571
    Chaini5 – 510506Cytochrome P450 4A4PRO_0000003572Add
    BLAST

    Expressioni

    Inductioni

    P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

    Interactioni

    Protein-protein interaction databases

    STRINGi9986.ENSOCUP00000001485.

    Structurei

    3D structure databases

    ProteinModelPortaliP10611.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei11 – 3121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000233833.
    HOVERGENiHBG000182.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P10611-1 [UniParc]FASTAAdd to Basket

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    MSVSALSPTR LPGSLSGLLQ VAALLGLLLL LLKAAQLYLH RQWLLRALQQ    50
    FPCPPFHWLL GHSREFQNDQ ELERIQKWVE KFPGACPWWL SGNKARLLVY 100
    DPDYLKVILG RSDPKAPRNY KLMTPWIGYG LLLLDGQTWF QHRRMLTPAF 150
    HYDILKPYVG LMVDSVQIML DRWEQLISQD SSLEIFQHVS LMTLDTIMKC 200
    AFSYQGSVQL DRNSHSYIQA INDLNNLVFY RARNVFHQSD FLYRLSPEGR 250
    LFHRACQLAH EHTDRVIQQR KAQLQQEGEL EKVRRKRRLD FLDVLLFAKM 300
    ENGSSLSDQD LRAEVDTFMF EGHDTTASGV SWIFYALATH PEHQHRCREE 350
    IQGLLGDGAS ITWEHLDQMP YTTMCIKEAL RLYPPVPSVT RQLSKPVTFP 400
    DGRSLPKGVI LFLSIYGLHY NPKVWQNPEV FDPFRFAPDS AYHSHAFLPF 450
    SGGARNCIGK QFAMRELKVA VALTLVRFEL LPDPTRIPIP IARVVLKSKN 500
    GIHLRLRKLH 510
    Length:510
    Mass (Da):58,930
    Last modified:July 19, 2004 - v3
    Checksum:i50CB1DE9D9D7FD5D
    GO

    Sequence cautioni

    The sequence AAA31228.1 differs from that shown. Reason: Frameshift at position 23.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L04758 Genomic DNA. Translation: AAA31228.1. Frameshift.
    J02818 mRNA. Translation: AAA31232.1.
    PIRiS32315. A29368.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L04758 Genomic DNA. Translation: AAA31228.1 . Frameshift.
    J02818 mRNA. Translation: AAA31232.1 .
    PIRi S32315. A29368.

    3D structure databases

    ProteinModelPortali P10611.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9986.ENSOCUP00000001485.

    Chemistry

    BindingDBi P10611.
    ChEMBLi CHEMBL4743.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000233833.
    HOVERGENi HBG000182.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rabbit prostaglandin omega-hydroxylase (CYP4A4): gene structure and expression."
      Palmer C.N., Griffin K.J., Johnson E.F.
      Arch. Biochem. Biophys. 300:670-676(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-65.
      Strain: III/J.
    2. "cDNA cloning and inducible expression during pregnancy of the mRNA for rabbit pulmonary prostaglandin omega-hydroxylase (cytochrome P-450p-2)."
      Matsubara S., Yamamoto S., Sogawa K., Yokotani N., Fujii-Kuriyama Y., Haniu M., Shively J.E., Gotoh O., Kusunose E., Kusunose M.
      J. Biol. Chem. 262:13366-13371(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 21-510, PROTEIN SEQUENCE OF 5-29.
    3. "Purification and characterization of two forms of fatty acid omega-hydroxylase cytochrome P-450 from rabbit kidney cortex microsomes."
      Yoshimura R., Kusunose E., Yokotani N., Yamamoto S., Kubota I., Kusunose M.
      J. Biochem. 108:544-548(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiCP4A4_RABIT
    AccessioniPrimary (citable) accession number: P10611
    Secondary accession number(s): Q02927
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 101 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3