Cytochrome P450 4A4 precursor - Oryctolagus cuniculus (Rabbit)

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P10611 (CP4A4_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cytochrome P450 4A4
EC=1.14.14.1

Alternative name(s):
CYPIVA4
Cytochrome P450-P-2
Prostaglandin omega-hydroxylase

Gene names

Name:

CYP4A4

Organism

Oryctolagus cuniculus (Rabbit) [Reference proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Catalyzes the omega- and (omega-1)-hydroxylation of fatty acids. Ref.3
Catalytic activity	RH + reduced flavoprotein + O₂ = ROH + oxidized flavoprotein + H₂O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein.
Induction	P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.
Sequence similarities	Belongs to the cytochrome P450 family.
Sequence caution	The sequence AAA31228.1 differs from that shown. Reason: Frameshift at position 23.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Propeptide

1 – 4

Probable

PRO_0000003571

Chain

5 – 510

506

Cytochrome P450 4A4

PRO_0000003572

Regions

Transmembrane

11 – 31

Helical; Potential

Sites

Metal binding

457

Iron (heme axial ligand)

Binding site

321

Heme (covalent; via 1 link) By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P10611 [UniParc].

Last modified July 19, 2004. Version 3.
Checksum: 50CB1DE9D9D7FD5D
Show »

FASTA

510

58,930

        10         20         30         40         50         60 
MSVSALSPTR LPGSLSGLLQ VAALLGLLLL LLKAAQLYLH RQWLLRALQQ FPCPPFHWLL 

        70         80         90        100        110        120 
GHSREFQNDQ ELERIQKWVE KFPGACPWWL SGNKARLLVY DPDYLKVILG RSDPKAPRNY 

       130        140        150        160        170        180 
KLMTPWIGYG LLLLDGQTWF QHRRMLTPAF HYDILKPYVG LMVDSVQIML DRWEQLISQD 

       190        200        210        220        230        240 
SSLEIFQHVS LMTLDTIMKC AFSYQGSVQL DRNSHSYIQA INDLNNLVFY RARNVFHQSD 

       250        260        270        280        290        300 
FLYRLSPEGR LFHRACQLAH EHTDRVIQQR KAQLQQEGEL EKVRRKRRLD FLDVLLFAKM 

       310        320        330        340        350        360 
ENGSSLSDQD LRAEVDTFMF EGHDTTASGV SWIFYALATH PEHQHRCREE IQGLLGDGAS 

       370        380        390        400        410        420 
ITWEHLDQMP YTTMCIKEAL RLYPPVPSVT RQLSKPVTFP DGRSLPKGVI LFLSIYGLHY 

       430        440        450        460        470        480 
NPKVWQNPEV FDPFRFAPDS AYHSHAFLPF SGGARNCIGK QFAMRELKVA VALTLVRFEL 

       490        500        510 
LPDPTRIPIP IARVVLKSKN GIHLRLRKLH

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References

[1]	"Rabbit prostaglandin omega-hydroxylase (CYP4A4): gene structure and expression." Palmer C.N., Griffin K.J., Johnson E.F. Arch. Biochem. Biophys. 300:670-676(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-65. Strain: III/J.
[2]	"cDNA cloning and inducible expression during pregnancy of the mRNA for rabbit pulmonary prostaglandin omega-hydroxylase (cytochrome P-450p-2)." Matsubara S., Yamamoto S., Sogawa K., Yokotani N., Fujii-Kuriyama Y., Haniu M., Shively J.E., Gotoh O., Kusunose E., Kusunose M. J. Biol. Chem. 262:13366-13371(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 21-510, PROTEIN SEQUENCE OF 5-29.
[3]	"Purification and characterization of two forms of fatty acid omega-hydroxylase cytochrome P-450 from rabbit kidney cortex microsomes." Yoshimura R., Kusunose E., Yokotani N., Yamamoto S., Kubota I., Kusunose M. J. Biochem. 108:544-548(1990) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L04758 Genomic DNA. Translation: AAA31228.1. Frameshift. J02818 mRNA. Translation: AAA31232.1.
PIR	A29368. S32315.
3D structure databases
ProteinModelPortal	P10611.
ModBase	Search...
Protein-protein interaction databases
STRING	9986.ENSOCUP00000001485.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG2124.
HOGENOM	HOG000233833.
HOVERGEN	HBG000182.
OrthoDB	EOG4ZS93G.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P10611.
ChEMBL	CHEMBL4743.

Entry information

Entry name

CP4A4_RABIT

Accession

Primary (citable) accession number: P10611
Secondary accession number(s): Q02927

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 19, 2004
Last modified:	April 3, 2013
This is version 96 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents