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P10611 (CP4A4_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 4A4

EC=1.14.14.1
Alternative name(s):
CYPIVA4
Cytochrome P450-P-2
Prostaglandin omega-hydroxylase
Gene names
Name:CYP4A4
OrganismOryctolagus cuniculus (Rabbit) [Reference proteome]
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus

Protein attributes

Sequence length510 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Catalyzes the omega- and (omega-1)-hydroxylation of fatty acids. Ref.3

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein.

Induction

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence caution

The sequence AAA31228.1 differs from that shown. Reason: Frameshift at position 23.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 44 Probable
PRO_0000003571
Chain5 – 510506Cytochrome P450 4A4
PRO_0000003572

Regions

Transmembrane11 – 3121Helical; Potential

Sites

Metal binding4571Iron (heme axial ligand)
Binding site3211Heme (covalent; via 1 link) By similarity

Sequences

Sequence LengthMass (Da)Tools
P10611 [UniParc].

Last modified July 19, 2004. Version 3.
Checksum: 50CB1DE9D9D7FD5D

FASTA51058,930
        10         20         30         40         50         60 
MSVSALSPTR LPGSLSGLLQ VAALLGLLLL LLKAAQLYLH RQWLLRALQQ FPCPPFHWLL 

        70         80         90        100        110        120 
GHSREFQNDQ ELERIQKWVE KFPGACPWWL SGNKARLLVY DPDYLKVILG RSDPKAPRNY 

       130        140        150        160        170        180 
KLMTPWIGYG LLLLDGQTWF QHRRMLTPAF HYDILKPYVG LMVDSVQIML DRWEQLISQD 

       190        200        210        220        230        240 
SSLEIFQHVS LMTLDTIMKC AFSYQGSVQL DRNSHSYIQA INDLNNLVFY RARNVFHQSD 

       250        260        270        280        290        300 
FLYRLSPEGR LFHRACQLAH EHTDRVIQQR KAQLQQEGEL EKVRRKRRLD FLDVLLFAKM 

       310        320        330        340        350        360 
ENGSSLSDQD LRAEVDTFMF EGHDTTASGV SWIFYALATH PEHQHRCREE IQGLLGDGAS 

       370        380        390        400        410        420 
ITWEHLDQMP YTTMCIKEAL RLYPPVPSVT RQLSKPVTFP DGRSLPKGVI LFLSIYGLHY 

       430        440        450        460        470        480 
NPKVWQNPEV FDPFRFAPDS AYHSHAFLPF SGGARNCIGK QFAMRELKVA VALTLVRFEL 

       490        500        510 
LPDPTRIPIP IARVVLKSKN GIHLRLRKLH 

« Hide

References

[1]"Rabbit prostaglandin omega-hydroxylase (CYP4A4): gene structure and expression."
Palmer C.N., Griffin K.J., Johnson E.F.
Arch. Biochem. Biophys. 300:670-676(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-65.
Strain: III/J.
[2]"cDNA cloning and inducible expression during pregnancy of the mRNA for rabbit pulmonary prostaglandin omega-hydroxylase (cytochrome P-450p-2)."
Matsubara S., Yamamoto S., Sogawa K., Yokotani N., Fujii-Kuriyama Y., Haniu M., Shively J.E., Gotoh O., Kusunose E., Kusunose M.
J. Biol. Chem. 262:13366-13371(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 21-510, PROTEIN SEQUENCE OF 5-29.
[3]"Purification and characterization of two forms of fatty acid omega-hydroxylase cytochrome P-450 from rabbit kidney cortex microsomes."
Yoshimura R., Kusunose E., Yokotani N., Yamamoto S., Kubota I., Kusunose M.
J. Biochem. 108:544-548(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L04758 Genomic DNA. Translation: AAA31228.1. Frameshift.
J02818 mRNA. Translation: AAA31232.1.
PIRA29368. S32315.

3D structure databases

ProteinModelPortalP10611.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9986.ENSOCUP00000001485.

Chemistry

BindingDBP10611.
ChEMBLCHEMBL4743.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000233833.
HOVERGENHBG000182.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP4A4_RABIT
AccessionPrimary (citable) accession number: P10611
Secondary accession number(s): Q02927
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 19, 2004
Last modified: February 19, 2014
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families