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Protein

Invertase 1

Gene

SUC1

Organism
Saccharomyces cerevisiae (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei42 – 421PROSITE-ProRule annotation
Binding sitei60 – 601SubstrateBy similarity
Binding sitei223 – 2231SubstrateBy similarity
Binding sitei311 – 3111SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH32. Glycoside Hydrolase Family 32.
mycoCLAPiSUC32A_YEAST.

Names & Taxonomyi

Protein namesi
Recommended name:
Invertase 1 (EC:3.2.1.26)
Alternative name(s):
Beta-fructofuranosidase 1
Saccharase
Gene namesi
Name:SUC1
OrganismiSaccharomyces cerevisiae (Baker's yeast)
Taxonomic identifieri4932 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Organism-specific databases

SGDiS000029531. SUC1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei8263. Sac c Invertase.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Add
BLAST
Chaini20 – 532513Invertase 1PRO_0000033398Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi23 – 231N-linked (GlcNAc...); in isoform SecretedSequence analysis
Glycosylationi64 – 641N-linked (GlcNAc...); in isoform SecretedSequence analysis
Glycosylationi111 – 1111N-linked (GlcNAc...); in isoform SecretedSequence analysis
Glycosylationi112 – 1121N-linked (GlcNAc...); in isoform SecretedSequence analysis
Glycosylationi118 – 1181N-linked (GlcNAc...); in isoform SecretedSequence analysis
Glycosylationi165 – 1651N-linked (GlcNAc...); in isoform SecretedSequence analysis
Glycosylationi275 – 2751N-linked (GlcNAc...); in isoform SecretedSequence analysis
Glycosylationi356 – 3561N-linked (GlcNAc...); in isoform SecretedSequence analysis
Glycosylationi369 – 3691N-linked (GlcNAc...); in isoform SecretedSequence analysis
Glycosylationi384 – 3841N-linked (GlcNAc...); in isoform SecretedSequence analysis
Glycosylationi398 – 3981N-linked (GlcNAc...); in isoform SecretedSequence analysis
Glycosylationi512 – 5121N-linked (GlcNAc...); in isoform SecretedSequence analysis

Post-translational modificationi

Isoform Secreted is glycosylated. Isoform Intracellular is not glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP10594.

Interactioni

Protein-protein interaction databases

STRINGi4932.YIL162W.

Structurei

3D structure databases

ProteinModelPortaliP10594.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni39 – 424Substrate bindingBy similarity
Regioni102 – 1032Substrate bindingBy similarity
Regioni170 – 1712Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 32 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0228. Eukaryota.
COG1621. LUCA.

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
2.60.120.560. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR001362. Glyco_hydro_32.
IPR018053. Glyco_hydro_32_AS.
IPR013189. Glyco_hydro_32_C.
IPR013148. Glyco_hydro_32_N.
IPR023296. Glyco_hydro_beta-prop.
IPR011040. Sialidases.
[Graphical view]
PfamiPF08244. Glyco_hydro_32C. 1 hit.
PF00251. Glyco_hydro_32N. 1 hit.
[Graphical view]
SMARTiSM00640. Glyco_32. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF75005. SSF75005. 1 hit.
PROSITEiPS00609. GLYCOSYL_HYDROL_F32. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Secreted (identifier: P10594-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLQAFLFLL AGFAAKISAS MTNETSDRPL VHFTPNKGWM NDPNGLWYDA
60 70 80 90 100
KEGKWHLYFQ YNPNDTVWGL PLFWGHATSD DLTHWQDEPV AIAPKRKDSG
110 120 130 140 150
AYSGSMVIDY NNTSGFFNDT IDPRQRCVAI WTYNTPESEE QYISYSLDGG
160 170 180 190 200
YTFTEYQKNP VLAANSTQFR DPKVFWYEPS KKWIMTAAKS QDYKIEIYSS
210 220 230 240 250
DDLKSWKLES AFANEGFLGY QYECPGLIEV PSEQDPSKSH WVMFISINPG
260 270 280 290 300
APAGGSFNQY FVGSFNGHHF EAFDNQSRVV DFGKDYYALQ TFFNTDPTYG
310 320 330 340 350
SALGIAWASN WEYSAFVPSN PWRSSMSLVR PFSLNTEYQA NPETELINLK
360 370 380 390 400
AEPILNISSA GPWSRFATNT TLTKANSYNV DLSNSTGTLE FELVYAVNTT
410 420 430 440 450
QTISKSVFAD LSLWFKGLED PEEYLRMGFE VSASSFFLDR GNSKVKFVKE
460 470 480 490 500
NPYFTNRMSV NNQPFKSEND LSYYKVYGLL DQNILELYFN DGDVVSTNTY
510 520 530
FMTTGNALGS VNMTTGVDNL FYIDKFQVRE VK
Length:532
Mass (Da):60,570
Last modified:July 1, 1989 - v1
Checksum:i3280EF260EA01606
GO
Isoform Intracellular (identifier: P10594-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.

Note: Produced by alternative initiation at Met-21 of isoform Secreted.
Show »
Length:512
Mass (Da):58,476
Checksum:iA372DE49187249A1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2020Missing in isoform Intracellular. CuratedVSP_019610Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07570 Genomic DNA. Translation: CAA30457.1.
PIRiS27372.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07570 Genomic DNA. Translation: CAA30457.1.
PIRiS27372.

3D structure databases

ProteinModelPortaliP10594.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4932.YIL162W.

Protein family/group databases

Allergomei8263. Sac c Invertase.
CAZyiGH32. Glycoside Hydrolase Family 32.
mycoCLAPiSUC32A_YEAST.

Proteomic databases

PaxDbiP10594.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

SGDiS000029531. SUC1.

Phylogenomic databases

eggNOGiKOG0228. Eukaryota.
COG1621. LUCA.

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
2.60.120.560. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR001362. Glyco_hydro_32.
IPR018053. Glyco_hydro_32_AS.
IPR013189. Glyco_hydro_32_C.
IPR013148. Glyco_hydro_32_N.
IPR023296. Glyco_hydro_beta-prop.
IPR011040. Sialidases.
[Graphical view]
PfamiPF08244. Glyco_hydro_32C. 1 hit.
PF00251. Glyco_hydro_32N. 1 hit.
[Graphical view]
SMARTiSM00640. Glyco_32. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF75005. SSF75005. 1 hit.
PROSITEiPS00609. GLYCOSYL_HYDROL_F32. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Structural analysis of the 5' regions of yeast SUC genes revealed analogous palindromes in SUC, MAL and GAL."
    Hohmann S., Gozalbo D.
    Mol. Gen. Genet. 211:446-454(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-73.
  2. Hohmann S.
    Submitted (NOV-1988) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "SUC1 gene of Saccharomyces: a structural gene for the large (glycoprotein) and small (carbohydrate-free) forms of invertase."
    Rodriguez L., Lampen J.O., MacKay V.L.
    Mol. Cell. Biol. 1:469-474(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF ISOFORMS, GLYCOSYLATION.

Entry informationi

Entry nameiINV1_YEASX
AccessioniPrimary (citable) accession number: P10594
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 11, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.