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Reviewed, UniProtKB/Swiss-Prot P10594 (INV1_YEAST)

Last modified October 13, 2009. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Invertase 1
    EC=3.2.1.26
Alternative name(s):
    Beta-fructofuranosidase 1
    Saccharase
Gene names
Name: SUC1
OrganismSaccharomyces cerevisiae (Baker's yeast)
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length532 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.

Post-translational modification

The enzyme exists in two states: a glycosylated secreted form, and a non-glycosylated intracellular form. Ref.3

Sequence similarities

Belongs to the glycosyl hydrolase 32 family.

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Ontologies

Keywords
   Coding sequence diversityAlternative initiation
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular functionbeta-fructofuranosidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform Secreted (identifier: P10594-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Cytoplasmic (identifier: P10594-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919
Chain20 – 532513Invertase 1
PRO_0000033398

Regions

Region39 – 424Substrate binding By similarity
Region102 – 1032Substrate binding By similarity
Region170 – 1712Substrate binding By similarity

Sites

Active site421 By similarity
Binding site601Substrate By similarity
Binding site2231Substrate By similarity
Binding site3111Substrate By similarity

Amino acid modifications

Glycosylation231N-linked (GlcNAc...); in isoform Secreted Potential
Glycosylation641N-linked (GlcNAc...); in isoform Secreted Potential
Glycosylation1111N-linked (GlcNAc...); in isoform Secreted Potential
Glycosylation1121N-linked (GlcNAc...); in isoform Secreted Potential
Glycosylation1181N-linked (GlcNAc...); in isoform Secreted Potential
Glycosylation1651N-linked (GlcNAc...); in isoform Secreted Potential
Glycosylation2751N-linked (GlcNAc...); in isoform Secreted Potential
Glycosylation3561N-linked (GlcNAc...); in isoform Secreted Potential
Glycosylation3691N-linked (GlcNAc...); in isoform Secreted Potential
Glycosylation3841N-linked (GlcNAc...); in isoform Secreted Potential
Glycosylation3981N-linked (GlcNAc...); in isoform Secreted Potential
Glycosylation5121N-linked (GlcNAc...); in isoform Secreted Potential

Natural variations

Alternative sequence1 – 2020Missing in isoform Cytoplasmic.
VSP_019610

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Sequences

Sequence LengthMass (Da)Tools
Isoform Secreted [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 3280EF260EA01606

FASTA53260,570
        10         20         30         40         50         60 
MLLQAFLFLL AGFAAKISAS MTNETSDRPL VHFTPNKGWM NDPNGLWYDA KEGKWHLYFQ 

        70         80         90        100        110        120 
YNPNDTVWGL PLFWGHATSD DLTHWQDEPV AIAPKRKDSG AYSGSMVIDY NNTSGFFNDT 

       130        140        150        160        170        180 
IDPRQRCVAI WTYNTPESEE QYISYSLDGG YTFTEYQKNP VLAANSTQFR DPKVFWYEPS 

       190        200        210        220        230        240 
KKWIMTAAKS QDYKIEIYSS DDLKSWKLES AFANEGFLGY QYECPGLIEV PSEQDPSKSH 

       250        260        270        280        290        300 
WVMFISINPG APAGGSFNQY FVGSFNGHHF EAFDNQSRVV DFGKDYYALQ TFFNTDPTYG 

       310        320        330        340        350        360 
SALGIAWASN WEYSAFVPSN PWRSSMSLVR PFSLNTEYQA NPETELINLK AEPILNISSA 

       370        380        390        400        410        420 
GPWSRFATNT TLTKANSYNV DLSNSTGTLE FELVYAVNTT QTISKSVFAD LSLWFKGLED 

       430        440        450        460        470        480 
PEEYLRMGFE VSASSFFLDR GNSKVKFVKE NPYFTNRMSV NNQPFKSEND LSYYKVYGLL 

       490        500        510        520        530 
DQNILELYFN DGDVVSTNTY FMTTGNALGS VNMTTGVDNL FYIDKFQVRE VK

« Hide

Isoform Cytoplasmic.

Checksum: A372DE49187249A1
Show »

FASTA51258,476

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References

[1]"Structural analysis of the 5' regions of yeast SUC genes revealed analogous palindromes in SUC, MAL and GAL."
Hohmann S., Gozalbo D.
Mol. Gen. Genet. 211:446-454(1988) [PubMed: 2835632] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-73.
[2]Hohmann S.
Submitted (NOV-1988) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"SUC1 gene of Saccharomyces: a structural gene for the large (glycoprotein) and small (carbohydrate-free) forms of invertase."
Rodriguez L., Lampen J.O., MacKay V.L.
Mol. Cell. Biol. 1:469-474(1981) [PubMed: 6765604] [Abstract]
Cited for: IDENTIFICATION OF ISOFORMS, GLYCOSYLATION.
+Additional computationally mapped references.

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Cross-references

Sequence databases

X07570 Genomic DNA. Translation: CAA30457.1.
PIRS27372.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGP10594.

Protein family/group databases

CAZyGH32. Glycoside Hydrolase Family 32.

Genome annotation databases

EnsemblYIL162W; YIL162W; YIL162W; Saccharomyces cerevisiae. [Genome view]

Organism-specific databases

SGDS000029531. SUC1.

Enzyme and pathway databases

BRENDA3.2.1.26. 250.

Gene expression databases

ArrayExpressP10594.
GenevestigatorP10594.
GermOnlineYIL162W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR001362. Glyco_hydro_32.
IPR018053. Glyco_hydro_32_AS.
IPR013189. Glyco_hydro_32_C.
IPR013148. Glyco_hydro_32_N.
[Graphical view]
PfamPF08244. Glyco_hydro_32C. 1 hit.
PF00251. Glyco_hydro_32N. 1 hit.
[Graphical view]
SMARTSM00640. Glyco_32. 1 hit.
[Graphical view]
PROSITEPS00609. GLYCOSYL_HYDROL_F32. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameINV1_YEAST
AccessionPrimary (citable) accession number: P10594
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 13, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents