Reviewed, UniProtKB/Swiss-Prot P10593 (ALB3_MAIZE)

Names and origin

Protein names

Recommended name:
    Albumin b-32
    EC=3.2.2.22
Alternative name(s):
    Protein opaque-6
    rRNA N-glycosidase

Gene names

Name:

O6

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	A possible regulatory factor for the synthesis of zeins, the major group of storage proteins.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Tissue specificity	Endosperm.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Cytoplasm
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Albumin b-32		PRO_0000221403
Regions
	Compositional bias	182 – 188	7	Poly-Ala
	Compositional bias	286 – 294	9	Poly-Ala
Experimental info
	Sequence conflict	269	1	D → N in CAA30797. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P10593-1 [UniParc]. Last modified October 1, 1993. Version 2. Checksum: 24003521CEF91790	FASTA	303	32,428
	10 20 30 40 50 60 MAETNPELSD LMAQTNKKIV PKFTEIFPVE DVNYPYSAFI ASVRKDVIKH CTDHKGIFQP 70 80 90 100 110 120 VLPPEKKVPE LWFYTELKTR TSSITLAIRM DNLYLVGFRT PGGVWWELAR PATPTSSATT 130 140 150 160 170 180 PGGSASAAGT RTSSATRVWR PSPWAARDDQ GRQRPGEEEE DGDTGGGGGA DADADAGGAE 190 200 210 220 230 240 LAAAAAAADP QADTKSKLVK LVVMVCEGLR FNTLSRTVDA GFNSQHGVTL TVTQGKQVQK 250 260 270 280 290 300 WDRISKAAFE WADHPTAVIP DMQKLGIKDK NEAARIVALV KNQTTAAAAA ATAASADNDD DEA

References

[1]	"The b-32 protein from maize endosperm: characterization of genomic sequences encoding two alternative central domains." Hartings H., Lazzaroni N., Marsan P.A., Aragay A., Thompson R., Salamini F., di Fonzo N., Palau J., Motto M. Plant Mol. Biol. 14:1031-1040(1990) [PubMed: 2102870] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The b-32 protein from maize endosperm, an albumin regulated by the O2 locus: nucleic acid (cDNA) and amino acid sequences." di Fonzo N., Hartings H., Brembilla M., Motto M., Soave C., Navarro E., Palau J., Rhode W., Salamini F. Mol. Gen. Genet. 212:481-487(1988) [PubMed: 3419419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Endosperm.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X54212 mRNA. Translation: CAA38124.1. X07987 mRNA. Translation: CAA30797.1.
PIR	S03172.
RefSeq	NP_001105428.1.
UniGene	Zm.97201
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	542383.
Organism-specific databases
Gramene	P10593.
MaizeGDB	30000.
Enzyme and pathway databases
BRENDA	3.2.2.22. 289.
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. IPR016138. Ribosome_inactivat_prot_sub1. IPR017989. Ribosome_inactivat_prot_subgr. [Graphical view]
Gene3D	G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit.
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PRINTS	PR00396. SHIGARICIN.
PROSITE	PS00275. SHIGA_RICIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALB3_MAIZE

Accession

Primary (citable) accession number: P10593

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)