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P10592

- HSP72_YEAST

UniProt

P10592 - HSP72_YEAST

Protein

Heat shock protein SSA2

Gene

SSA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 161 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    May play a role in the transport of polypeptides both across the mitochondrial membranes and into the endoplasmic reticulum. A functional difference between SSA1 and SSA2 proteins is expected. SSA2 can participate in the ATP-dependent disassembly of clathrin-coated vesicles.1 Publication

    GO - Molecular functioni

    1. ATPase activity Source: SGD
    2. ATP binding Source: SGD
    3. protein binding Source: IntAct
    4. unfolded protein binding Source: SGD

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. protein folding Source: SGD
    3. response to stress Source: UniProtKB-KW
    4. SRP-dependent cotranslational protein targeting to membrane, translocation Source: SGD

    Keywords - Biological processi

    Stress response

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32128-MONOMER.
    ReactomeiREACT_219346. HSF1-dependent transactivation.
    REACT_221137. Attenuation phase.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Heat shock protein SSA2
    Gene namesi
    Name:SSA2
    Ordered Locus Names:YLL024C
    ORF Names:L0931
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    SGDiS000003947. SSA2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. cytosol Source: SGD
    3. extracellular region Source: UniProtKB-KW
    4. fungal-type cell wall Source: SGD
    5. fungal-type vacuole membrane Source: SGD
    6. mitochondrion Source: SGD
    7. polysome Source: SGD

    Keywords - Cellular componenti

    Cell wall, Cytoplasm, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 639638Heat shock protein SSA2PRO_0000078386Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine2 Publications
    Modified residuei20 – 201Phosphoserine2 Publications
    Modified residuei551 – 5511PhosphoserineBy similarity
    Cross-linki556 – 556Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
    Modified residuei603 – 6031PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiP10592.
    PaxDbiP10592.
    PeptideAtlasiP10592.
    PRIDEiP10592.

    2D gel databases

    COMPLUYEAST-2DPAGEP10592.
    SWISS-2DPAGEP10592.

    Expressioni

    Gene expression databases

    GenevestigatoriP10592.

    Interactioni

    Subunit structurei

    Binds human HTN3/histatin-5, a peptide from saliva, and mediates its fungicidal activity. Interacts with NAP1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    COQ1P189002EBI-8603,EBI-4912
    ESS1P226962EBI-8603,EBI-6679
    HSP82P028292EBI-8603,EBI-8659
    MDH1P175052EBI-8603,EBI-10594

    Protein-protein interaction databases

    BioGridi31229. 348 interactions.
    DIPiDIP-2265N.
    IntActiP10592. 969 interactions.
    MINTiMINT-8285323.
    STRINGi4932.YLL024C.

    Structurei

    3D structure databases

    ProteinModelPortaliP10592.
    SMRiP10592. Positions 3-608.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the heat shock protein 70 family.Curated

    Phylogenomic databases

    eggNOGiCOG0443.
    GeneTreeiENSGT00750000117237.
    HOGENOMiHOG000228135.
    KOiK03283.
    OrthoDBiEOG728916.

    Family and domain databases

    Gene3Di1.20.1270.10. 1 hit.
    2.60.34.10. 1 hit.
    InterProiIPR018181. Heat_shock_70_CS.
    IPR029048. HSP70_C.
    IPR029047. HSP70_peptide-bd.
    IPR013126. Hsp_70_fam.
    [Graphical view]
    PfamiPF00012. HSP70. 1 hit.
    [Graphical view]
    PRINTSiPR00301. HEATSHOCK70.
    SUPFAMiSSF100920. SSF100920. 1 hit.
    SSF100934. SSF100934. 1 hit.
    PROSITEiPS00297. HSP70_1. 1 hit.
    PS00329. HSP70_2. 1 hit.
    PS01036. HSP70_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P10592-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKAVGIDLG TTYSCVAHFS NDRVDIIAND QGNRTTPSFV GFTDTERLIG    50
    DAAKNQAAMN PANTVFDAKR LIGRNFNDPE VQGDMKHFPF KLIDVDGKPQ 100
    IQVEFKGETK NFTPEQISSM VLGKMKETAE SYLGAKVNDA VVTVPAYFND 150
    SQRQATKDAG TIAGLNVLRI INEPTAAAIA YGLDKKGKEE HVLIFDLGGG 200
    TFDVSLLSIE DGIFEVKATA GDTHLGGEDF DNRLVNHFIQ EFKRKNKKDL 250
    STNQRALRRL RTACERAKRT LSSSAQTSVE IDSLFEGIDF YTSITRARFE 300
    ELCADLFRST LDPVEKVLRD AKLDKSQVDE IVLVGGSTRI PKVQKLVTDY 350
    FNGKEPNRSI NPDEAVAYGA AVQAAILTGD ESSKTQDLLL LDVAPLSLGI 400
    ETAGGVMTKL IPRNSTIPTK KSEVFSTYAD NQPGVLIQVF EGERAKTKDN 450
    NLLGKFELSG IPPAPRGVPQ IEVTFDVDSN GILNVSAVEK GTGKSNKITI 500
    TNDKGRLSKE DIEKMVAEAE KFKEEDEKES QRIASKNQLE SIAYSLKNTI 550
    SEAGDKLEQA DKDAVTKKAE ETIAWLDSNT TATKEEFDDQ LKELQEVANP 600
    IMSKLYQAGG APEGAAPGGF PGGAPPAPEA EGPTVEEVD 639
    Length:639
    Mass (Da):69,470
    Last modified:January 23, 2007 - v3
    Checksum:i129049E21C9DD1F3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12927 Genomic DNA. Translation: CAA31394.1.
    Z73129 Genomic DNA. Translation: CAA97472.1.
    X97560 Genomic DNA. Translation: CAA66167.1.
    BK006945 Genomic DNA. Translation: DAA09296.1.
    PIRiS20139.
    RefSeqiNP_013076.1. NM_001181844.1.

    Genome annotation databases

    EnsemblFungiiYLL024C; YLL024C; YLL024C.
    GeneIDi850636.
    KEGGisce:YLL024C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12927 Genomic DNA. Translation: CAA31394.1 .
    Z73129 Genomic DNA. Translation: CAA97472.1 .
    X97560 Genomic DNA. Translation: CAA66167.1 .
    BK006945 Genomic DNA. Translation: DAA09296.1 .
    PIRi S20139.
    RefSeqi NP_013076.1. NM_001181844.1.

    3D structure databases

    ProteinModelPortali P10592.
    SMRi P10592. Positions 3-608.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31229. 348 interactions.
    DIPi DIP-2265N.
    IntActi P10592. 969 interactions.
    MINTi MINT-8285323.
    STRINGi 4932.YLL024C.

    2D gel databases

    COMPLUYEAST-2DPAGE P10592.
    SWISS-2DPAGE P10592.

    Proteomic databases

    MaxQBi P10592.
    PaxDbi P10592.
    PeptideAtlasi P10592.
    PRIDEi P10592.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLL024C ; YLL024C ; YLL024C .
    GeneIDi 850636.
    KEGGi sce:YLL024C.

    Organism-specific databases

    SGDi S000003947. SSA2.

    Phylogenomic databases

    eggNOGi COG0443.
    GeneTreei ENSGT00750000117237.
    HOGENOMi HOG000228135.
    KOi K03283.
    OrthoDBi EOG728916.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32128-MONOMER.
    Reactomei REACT_219346. HSF1-dependent transactivation.
    REACT_221137. Attenuation phase.

    Miscellaneous databases

    NextBioi 966561.
    PROi P10592.

    Gene expression databases

    Genevestigatori P10592.

    Family and domain databases

    Gene3Di 1.20.1270.10. 1 hit.
    2.60.34.10. 1 hit.
    InterProi IPR018181. Heat_shock_70_CS.
    IPR029048. HSP70_C.
    IPR029047. HSP70_peptide-bd.
    IPR013126. Hsp_70_fam.
    [Graphical view ]
    Pfami PF00012. HSP70. 1 hit.
    [Graphical view ]
    PRINTSi PR00301. HEATSHOCK70.
    SUPFAMi SSF100920. SSF100920. 1 hit.
    SSF100934. SSF100934. 1 hit.
    PROSITEi PS00297. HSP70_1. 1 hit.
    PS00329. HSP70_2. 1 hit.
    PS01036. HSP70_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The SSA1 and SSA2 genes of the yeast Saccharomyces cerevisiae."
      Slater M.R., Craig E.A.
      Nucleic Acids Res. 17:805-806(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The sequence of 32kb on the left arm of yeast chromosome XII reveals six known genes, a new member of the seripauperins family and a new ABC transporter homologous to the human multidrug resistance protein."
      Purnelle B., Goffeau A.
      Yeast 13:183-188(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: PROTEIN SEQUENCE OF 92-98 AND 326-342.
      Strain: ATCC 204508 / S288c.
    6. "Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae."
      Norbeck J., Blomberg A.
      FEMS Microbiol. Lett. 137:1-8(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 187-196.
      Strain: ATCC 38531 / Y41.
    7. "Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins."
      Garrels J.I., McLaughlin C.S., Warner J.R., Futcher B., Latter G.I., Kobayashi R., Schwender B., Volpe T., Anderson D.S., Mesquita-Fuentes R., Payne W.E.
      Electrophoresis 18:1347-1360(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION AT SER-2, PHOSPHORYLATION.
    8. "Candida albicans Ssa1/2p is the cell envelope binding protein for human salivary histatin 5."
      Li X.S., Reddy M.S., Baev D., Edgerton M.
      J. Biol. Chem. 278:28553-28561(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HUMAN HTN3.
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-556.
      Strain: SUB592.
    11. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Phosphorylation by casein kinase 2 regulates Nap1 localization and function."
      Calvert M.E.K., Keck K.M., Ptak C., Shabanowitz J., Hunt D.F., Pemberton L.F.
      Mol. Cell. Biol. 28:1313-1325(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NAP1, IDENTIFICATION BY MASS SPECTROMETRY.
    13. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    15. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    16. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHSP72_YEAST
    AccessioniPrimary (citable) accession number: P10592
    Secondary accession number(s): D6VXY0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 161 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 364000 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3