Reviewed,
UniProtKB/Swiss-Prot P10591 (HSP71_YEAST)
Last modified
June 16, 2009.
Version 107.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Heat shock protein SSA1 Alternative name(s): Heat shock protein YG100 | ||||
| Gene names |
| ||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4932 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 642 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | May play a role in the transport of polypeptides both across the mitochondrial membranes and into the endoplasmic reticulum. A functional difference between SSA1 and SSA2 proteins is expected. SSA1 can participate in the ATP-dependent disassembly of clathrin-coated vesicles. Ref.10 |
| Subunit structure | Binds human HTN3/histatin-5, a peptide from saliva, and mediates its fungicidal activity. Interacts with polyadenylate-binding protein PAB1 and Hsp70 chaperone SSA1 on translating ribosomes. Interacts with NAP1. Ref.10 Ref.9 |
| Subcellular location | |
| Miscellaneous | Present with 269000 molecules/cell in log phase SD medium. Ref.12 |
| Sequence similarities | Belongs to the heat shock protein 70 family. |
Ontologies
Binary interactions
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed | ||||||
| Chain | 2 – 642 | 641 | Heat shock protein SSA1 | PRO_0000078385 | |||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | N-acetylserine Ref.8 | ||||||
| Modified residue | 12 | 1 | Phosphothreonine Ref.14 | ||||||
| Modified residue | 35 | 1 | Phosphothreonine Ref.17 | ||||||
| Modified residue | 36 | 1 | Phosphothreonine Ref.17 | ||||||
| Modified residue | 43 | 1 | Phosphothreonine Ref.17 | ||||||
| Modified residue | 62 | 1 | Phosphoserine Ref.17 | ||||||
| Modified residue | 131 | 1 | Phosphoserine Ref.17 | ||||||
| Modified residue | 151 | 1 | Phosphoserine Ref.17 | ||||||
| Modified residue | 378 | 1 | Phosphothreonine Ref.17 | ||||||
| Modified residue | 459 | 1 | Phosphoserine Ref.17 | ||||||
| Modified residue | 486 | 1 | Phosphoserine Ref.17 | ||||||
| Modified residue | 492 | 1 | Phosphothreonine Ref.17 | ||||||
| Modified residue | 495 | 1 | Phosphoserine Ref.17 Ref.15 | ||||||
| Modified residue | 541 | 1 | Phosphoserine Ref.17 | ||||||
| Modified residue | 545 | 1 | Phosphoserine Ref.17 | ||||||
| Modified residue | 551 | 1 | Phosphoserine Ref.17 Ref.15 | ||||||
| Modified residue | 603 | 1 | Phosphoserine Ref.17 | ||||||
| Cross-link | 521 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.13 | |||||||
| Cross-link | 536 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.13 | |||||||
Experimental info | |||||||||
| Sequence conflict | 208 | 1 | S → F in AAC04952. Ref.3 | ||||||
| Sequence conflict | 418 | 1 | P → S in AAC04952. Ref.3 | ||||||
| Sequence conflict | 422 | 1 | S → F in AAC04952. Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The SSA1 and SSA2 genes of the yeast Saccharomyces cerevisiae." Slater M.R., Craig E.A. Nucleic Acids Res. 17:805-806(1989) [PubMed: 2644626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [2] | Slater M.R. Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 208; 418 AND 422. |
| [3] | "Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region." Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H. Yeast 10:535-541(1994) [PubMed: 7941740] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [4] | "The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed: 7731988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [5] | "Protein identifications for a Saccharomyces cerevisiae protein database." Garrels J.I., Futcher B., Kobayashi R., Latter G.I., Schwender B., Volpe T., Warner J.R., McLaughlin C.S. Electrophoresis 15:1466-1486(1994) [PubMed: 7895733] [Abstract] Cited for: PROTEIN SEQUENCE OF 92-98 AND 326-342. Strain: ATCC 204508 / S288c. |
| [6] | "Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae." Norbeck J., Blomberg A. FEMS Microbiol. Lett. 137:1-8(1996) [PubMed: 8935650] [Abstract] Cited for: PROTEIN SEQUENCE OF 187-196. Strain: ATCC 38531 / Y41. |
| [7] | "Transfer RNA splicing in Saccharomyces cerevisiae: defining the substrates." Ogden R.C., Lee M.-C., Knapp G. Nucleic Acids Res. 12:9367-9382(1984) [PubMed: 6096826] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 591-642. |
| [8] | "Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins." Garrels J.I., McLaughlin C.S., Warner J.R., Futcher B., Latter G.I., Kobayashi R., Schwender B., Volpe T., Anderson D.S., Mesquita-Fuentes R., Payne W.E. Electrophoresis 18:1347-1360(1997) [PubMed: 9298649] [Abstract] Cited for: ACETYLATION AT SER-2. |
| [9] | "The yeast hsp70 homologue Ssa is required for translation and interacts with Sis1 and Pab1 on translating ribosomes." Horton L.E., James P., Craig E.A., Hensold J.O. J. Biol. Chem. 276:14426-14433(2001) [PubMed: 11279042] [Abstract] Cited for: INTERACTION WITH PAB1 AND SIS1. |
| [10] | "Candida albicans Ssa1/2p is the cell envelope binding protein for human salivary histatin 5." Li X.S., Reddy M.S., Baev D., Edgerton M. J. Biol. Chem. 278:28553-28561(2003) [PubMed: 12761219] [Abstract] Cited for: FUNCTION, INTERACTION WITH HUMAN HTN3. |
| [11] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [12] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [13] | "A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-521 AND LYS-536, MASS SPECTROMETRY. |
| [14] | "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-12, MASS SPECTROMETRY. |
| [15] | "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-495 AND SER-551, MASS SPECTROMETRY. |
| [16] | "Phosphorylation by casein kinase 2 regulates Nap1 localization and function." Calvert M.E.K., Keck K.M., Ptak C., Shabanowitz J., Hunt D.F., Pemberton L.F. Mol. Cell. Biol. 28:1313-1325(2008) [PubMed: 18086883] [Abstract] Cited for: INTERACTION WITH NAP1, MASS SPECTROMETRY. |
| [17] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-35; THR-36; THR-43; SER-62; SER-131; SER-151; THR-378; SER-459; SER-486; THR-492; SER-495; SER-541; SER-545; SER-551 AND SER-603, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| X12926 Genomic DNA. Translation: CAA31393.1. L22015 Genomic DNA. Translation: AAC04952.1. | |
| PIR | HHBYA1. S43449. |
| RefSeq | NP_009396.2. |
3D structure databases | |
| HSSP | HSSP built from PDB template 3HSC based on UniProtKB P19120. |
| SMR | P10591. Positions 1-550. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:2253N. |
| IntAct | P10591. 278 interactions. |
2-D gel databases | |
| SWISS-2DPAGE | P10591. |
| COMPLUYEAST-2DPAGE | P10591. |
Proteomic databases | |
| PeptideAtlas | P10591. |
| PRIDE | P10591. |
Genome annotation databases | |
| Ensembl | YAL005C. Saccharomyces cerevisiae. [Contig view] |
| GeneID | 851259. |
| GenomeReviews | Gene locus YAL005C in contig U00091_GR. |
| KEGG | sce:YAL005C. |
Organism-specific databases | |
| CYGD | YAL005c. |
| SGD | S000000004. SSA1. |
| Yeast-GFP | Search... |
Phylogenomic databases | |
| HOGENOM | P10591. |
Gene expression databases | |
| ArrayExpress | P10591. |
| GermOnline | YAL005C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR018181. Heat_shock_70_CS. IPR001023. Hsp70. IPR013126. Hsp_70. [Graphical view] |
| PANTHER | PTHR19375. Hsp70. 1 hit. |
| Pfam | PF00012. HSP70. 1 hit. [Graphical view] |
| PRINTS | PR00301. HEATSHOCK70. |
| ProDom | PD000089. Hsp70. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| PROSITE | PS00297. HSP70_1. 1 hit. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 968213. |
Entry information
| Entry name | HSP71_YEAST | ||||||||
| Accession | Primary (citable) accession number: P10591 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome I Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names |

Clusters with


