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Protein

Heat shock protein SSA1

Gene

SSA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the transport of polypeptides both across the mitochondrial membranes and into the endoplasmic reticulum. A functional difference between SSA1 and SSA2 proteins is expected. SSA1 can participate in the ATP-dependent disassembly of clathrin-coated vesicles.1 Publication

Miscellaneous

Present with 269000 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • tRNA binding Source: SGD
  • unfolded protein binding Source: SGD

GO - Biological processi

  • clathrin coat disassembly Source: SGD
  • cytoplasmic translation Source: SGD
  • negative regulation of cell aging Source: SGD
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
  • protein folding Source: SGD
  • protein import into nucleus, translocation Source: SGD
  • protein polyubiquitination Source: SGD
  • protein refolding Source: SGD
  • protein targeting to mitochondrion Source: SGD
  • SRP-dependent cotranslational protein targeting to membrane, translocation Source: SGD
  • stress granule disassembly Source: SGD

Keywordsi

Biological processStress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28819-MONOMER.
ReactomeiR-SCE-3371453. Regulation of HSF1-mediated heat shock response.
R-SCE-3371497. HSP90 chaperone cycle for steroid hormone receptors (SHR).
R-SCE-3371568. Attenuation phase.
R-SCE-3371571. HSF1-dependent transactivation.
R-SCE-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-8876725. Protein methylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock protein SSA1
Alternative name(s):
Heat shock protein YG100
Gene namesi
Name:SSA1
Ordered Locus Names:YAL005C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAL005C.
SGDiS000000004. SSA1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell wall, Cytoplasm, Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5186.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000783852 – 642Heat shock protein SSA1Add BLAST641

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1
Modified residuei62PhosphoserineCombined sources1
Modified residuei551PhosphoserineCombined sources1
Cross-linki556Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei603PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP10591.
PRIDEiP10591.

2D gel databases

COMPLUYEAST-2DPAGEiP10591.
SWISS-2DPAGEiP10591.

PTM databases

iPTMnetiP10591.

Interactioni

Subunit structurei

Binds human HTN3/histatin-5, a peptide from saliva, and mediates its fungicidal activity. Interacts with polyadenylate-binding protein PAB1 and Hsp70 chaperone SSA1 on translating ribosomes. Interacts with NAP1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ADA2Q023368EBI-8591,EBI-2186
AHA1Q124493EBI-8591,EBI-37072
APE2P324543EBI-8591,EBI-2641
APE4P388213EBI-8591,EBI-6008
ATG2P538552EBI-8591,EBI-29212
CAM1P295473EBI-8591,EBI-6323
CAR2P079912EBI-8591,EBI-12430
CDC21P067852EBI-8591,EBI-19690
CDC28P005462EBI-8591,EBI-4253
CDC73Q066974EBI-8591,EBI-29913
CHC1P221373EBI-8591,EBI-4766
CKA2P194543EBI-8591,EBI-9548
CLG1P351903EBI-8591,EBI-4762
CLN3P133652EBI-8591,EBI-4490
COQ8P276972EBI-8591,EBI-2013
CPR7P471033EBI-8591,EBI-5436
CTF4Q014543EBI-8591,EBI-5209
DOP1Q039213EBI-8591,EBI-34442
ECM16Q042173EBI-8591,EBI-1820
EFT2P323243EBI-8591,EBI-6333
FES1P382605EBI-8591,EBI-21563
GET4Q121253EBI-8591,EBI-36940
GPP1P412773EBI-8591,EBI-7829
HAP1P0CS822EBI-8591,EBI-5419From Saccharomyces cerevisiae.
HRB1P389223EBI-8591,EBI-8541
HSC82P151083EBI-8591,EBI-8666
HSP78P334163EBI-8591,EBI-8680
HSP82P028295EBI-8591,EBI-8659
HXK1P048062EBI-8591,EBI-8732
LHS1P360163EBI-8591,EBI-10154
MAK21Q121763EBI-8591,EBI-10944
MEF2P396772EBI-8591,EBI-6361
NAB3P389963EBI-8591,EBI-11776
NEW1Q089723EBI-8591,EBI-32014
NMD2P387983EBI-8591,EBI-12071
NPL6P328323EBI-8591,EBI-12202
OTU1P435583EBI-8591,EBI-22837
OXP1P282732EBI-8591,EBI-27022
PAN3P361022EBI-8591,EBI-12895
PCL2P256933EBI-8591,EBI-4499
PFK1P168613EBI-8591,EBI-9428
PHO85P171573EBI-8591,EBI-13327
PNP1Q057883EBI-8591,EBI-13604
RIM20Q120332EBI-8591,EBI-38947
RPA190P109643EBI-8591,EBI-15730
RPB2P085183EBI-8591,EBI-15767
RPN2P325653EBI-8591,EBI-15919
RPT6Q019393EBI-8591,EBI-13914
RRP5Q050224EBI-8591,EBI-16011
RSC3Q066393EBI-8591,EBI-22058
RSC8P436093EBI-8591,EBI-23005
RTN1Q049473EBI-8591,EBI-38020
RUD3Q122342EBI-8591,EBI-31697
RVS161P253433EBI-8591,EBI-14490
SAM1P106593EBI-8591,EBI-10789
SGT2Q121184EBI-8591,EBI-31784
SIS1P252944EBI-8591,EBI-17244
SKI2P352073EBI-8591,EBI-1851
SPT7P351774EBI-8591,EBI-17958
SSA2P105923EBI-8591,EBI-8603
SSB1P114843EBI-8591,EBI-8627
SSE1P3258910EBI-8591,EBI-8648
SSE2P325903EBI-8591,EBI-8655
SUP35P054534EBI-8591,EBI-6540
TAF12Q037613EBI-8591,EBI-35097
TAF5P381296EBI-8591,EBI-18868
TAF9Q050274EBI-8591,EBI-27500
UBA2P524883EBI-8591,EBI-19710
UBP2Q014763EBI-8591,EBI-19826
UBP8P501026EBI-8591,EBI-19863
UGP1P328613EBI-8591,EBI-19987
URA2P072593EBI-8591,EBI-14372
URB1P342413EBI-8591,EBI-26595
UTP8P532763EBI-8591,EBI-23301
XDJ1P391022EBI-8591,EBI-20578
YAK1P146803EBI-8591,EBI-20777
YDJ1P254913EBI-8591,EBI-10420
YPK1P126883EBI-8591,EBI-29473

GO - Molecular functioni

  • unfolded protein binding Source: SGD

Protein-protein interaction databases

BioGridi31786. 772 interactors.
DIPiDIP-2253N.
IntActiP10591. 2752 interactors.
MINTiMINT-8285515.
STRINGi4932.YAL005C.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LCAX-ray2.19Q631-642[»]
ProteinModelPortaliP10591.
SMRiP10591.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10591.

Family & Domainsi

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Phylogenomic databases

GeneTreeiENSGT00890000139681.
HOGENOMiHOG000228135.
InParanoidiP10591.
KOiK03283.
OMAiAYTKNQD.
OrthoDBiEOG092C1VPN.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiView protein in InterPro
IPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
PfamiView protein in Pfam
PF00012. HSP70. 1 hit.
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiView protein in PROSITE
PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10591-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKAVGIDLG TTYSCVAHFA NDRVDIIAND QGNRTTPSFV AFTDTERLIG
60 70 80 90 100
DAAKNQAAMN PSNTVFDAKR LIGRNFNDPE VQADMKHFPF KLIDVDGKPQ
110 120 130 140 150
IQVEFKGETK NFTPEQISSM VLGKMKETAE SYLGAKVNDA VVTVPAYFND
160 170 180 190 200
SQRQATKDAG TIAGLNVLRI INEPTAAAIA YGLDKKGKEE HVLIFDLGGG
210 220 230 240 250
TFDVSLLSIE DGIFEVKATA GDTHLGGEDF DNRLVNHFIQ EFKRKNKKDL
260 270 280 290 300
STNQRALRRL RTACERAKRT LSSSAQTSVE IDSLFEGIDF YTSITRARFE
310 320 330 340 350
ELCADLFRST LDPVEKVLRD AKLDKSQVDE IVLVGGSTRI PKVQKLVTDY
360 370 380 390 400
FNGKEPNRSI NPDEAVAYGA AVQAAILTGD ESSKTQDLLL LDVAPLSLGI
410 420 430 440 450
ETAGGVMTKL IPRNSTIPTK KSEIFSTYAD NQPGVLIQVF EGERAKTKDN
460 470 480 490 500
NLLGKFELSG IPPAPRGVPQ IEVTFDVDSN GILNVSAVEK GTGKSNKITI
510 520 530 540 550
TNDKGRLSKE DIEKMVAEAE KFKEEDEKES QRIASKNQLE SIAYSLKNTI
560 570 580 590 600
SEAGDKLEQA DKDTVTKKAE ETISWLDSNT TASKEEFDDK LKELQDIANP
610 620 630 640
IMSKLYQAGG APGGAAGGAP GGFPGGAPPA PEAEGPTVEE VD
Length:642
Mass (Da):69,657
Last modified:January 23, 2007 - v4
Checksum:i249D8A3BC42527CA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti208S → F in AAC04952 (PubMed:7941740).Curated1
Sequence conflicti418P → S in AAC04952 (PubMed:7941740).Curated1
Sequence conflicti422S → F in AAC04952 (PubMed:7941740).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12926 Genomic DNA. Translation: CAA31393.1.
L22015 Genomic DNA. Translation: AAC04952.1.
BK006935 Genomic DNA. Translation: DAA06982.1.
PIRiS43449. HHBYA1.
RefSeqiNP_009396.2. NM_001178151.1.

Genome annotation databases

EnsemblFungiiYAL005C; YAL005C; YAL005C.
GeneIDi851259.
KEGGisce:YAL005C.

Similar proteinsi

Entry informationi

Entry nameiHSP71_YEAST
AccessioniPrimary (citable) accession number: P10591
Secondary accession number(s): D6VPL2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: September 27, 2017
This is version 194 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names