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P10589 (COT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 154. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
COUP transcription factor 1

Short name=COUP-TF1
Alternative name(s):
COUP transcription factor I
Short name=COUP-TF I
Nuclear receptor subfamily 2 group F member 1
V-erbA-related protein 3
Short name=EAR-3
Gene names
Name:NR2F1
Synonyms:EAR3, ERBAL3, TFCOUP1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG. Ref.5 Ref.6

Subunit structure

Binds DNA as dimer; homodimer and probable heterodimer with NR2F6. Interacts with COPS2. Ref.4 Ref.6

Subcellular location

Nucleus Probable.

Induction

Inhibited by gonadotropin in granulosa cells. Ref.6

Sequence similarities

Belongs to the nuclear hormone receptor family. NR2 subfamily.

Contains 1 nuclear receptor DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainZinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator
Receptor
Repressor
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcerebral cortex regionalization

Inferred from electronic annotation. Source: Ensembl

gene expression

Traceable author statement. Source: Reactome

intracellular receptor signaling pathway

Traceable author statement PubMed 8530078. Source: GOC

negative regulation of neuron projection development

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay Ref.5. Source: UniProtKB

neuron migration

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

signal transduction

Traceable author statement Ref.1. Source: ProtInc

transcription initiation from RNA polymerase II promoter

Traceable author statement. Source: Reactome

   Cellular_componentnucleoplasm

Traceable author statement. Source: Reactome

nucleus

Inferred from direct assay Ref.5. Source: UniProtKB

   Molecular_functionligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity

Traceable author statement PubMed 8530078. Source: ProtInc

protein binding

Inferred from physical interaction PubMed 11043578. Source: BHF-UCL

retinoic acid-responsive element binding

Inferred from electronic annotation. Source: Ensembl

sequence-specific DNA binding

Inferred from direct assay Ref.5. Source: UniProtKB

sequence-specific DNA binding transcription factor activity

Inferred from direct assay Ref.5. Source: UniProtKB

steroid hormone receptor activity

Inferred from electronic annotation. Source: InterPro

transcription coactivator activity

Traceable author statement Ref.1. Source: ProtInc

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 423423COUP transcription factor 1
PRO_0000053602

Regions

DNA binding83 – 15876Nuclear receptor
Zinc finger86 – 10621NR C4-type
Zinc finger122 – 14625NR C4-type
Compositional bias32 – 365Poly-Gly

Secondary structure

.............. 423
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P10589 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 6EE634BE96242731

FASTA42346,156
        10         20         30         40         50         60 
MAMVVSSWRD PQDDVAGGNP GGPNPAAQAA RGGGGGAGEQ QQQAGSGAPH TPQTPGQPGA 

        70         80         90        100        110        120 
PATPGTAGDK GQGPPGSGQS QQHIECVVCG DKSSGKHYGQ FTCEGCKSFF KRSVRRNLTY 

       130        140        150        160        170        180 
TCRANRNCPI DQHHRNQCQY CRLKKCLKVG MRREAVQRGR MPPTQPNPGQ YALTNGDPLN 

       190        200        210        220        230        240 
GHCYLSGYIS LLLRAEPYPT SRYGSQCMQP NNIMGIENIC ELAARLLFSA VEWARNIPFF 

       250        260        270        280        290        300 
PDLQITDQVS LLRLTWSELF VLNAAQCSMP LHVAPLLAAA GLHASPMSAD RVVAFMDHIR 

       310        320        330        340        350        360 
IFQEQVEKLK ALHVDSAEYS CLKAIVLFTS DACGLSDAAH IESLQEKSQC ALEEYVRSQY 

       370        380        390        400        410        420 
PNQPSRFGKL LLRLPSLRTV SSSVIEQLFF VRLVGKTPIE TLIRDMLLSG SSFNWPYMSI 


QCS 

« Hide

References

« Hide 'large scale' references
[1]"COUP transcription factor is a member of the steroid receptor superfamily."
Wang L.-H., Tsai S.Y., Cook R.G., Beattie W.G., Tsai M.-J., O'Malley B.W.
Nature 340:163-166(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-423, PARTIAL PROTEIN SEQUENCE.
[2]"Identification of two novel members of erbA superfamily by molecular cloning: the gene products of the two are highly related to each other."
Miyajima N., Kadowaki Y., Fukushige S., Shimizu S., Semba K., Yamanashi Y., Matsubara K., Toyoshima K., Yamamoto T.
Nucleic Acids Res. 16:11057-11074(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Lung.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung and Uterus.
[4]"Alien, a highly conserved protein with characteristics of a corepressor for members of the nuclear hormone receptor superfamily."
Dressel U., Thormeyer D., Altincicek B., Paululat A., Eggert M., Schneider S., Tenbaum S.P., Renkawitz R., Baniahmad A.
Mol. Cell. Biol. 19:3383-3394(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH COPS2.
[5]"Nuclear orphan receptors regulate transcription of the gene for the human luteinizing hormone receptor."
Zhang Y., Dufau M.L.
J. Biol. Chem. 275:2763-2770(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"EAR2 and EAR3/COUP-TFI regulate transcription of the rat LH receptor."
Zhang Y., Dufau M.L.
Mol. Endocrinol. 15:1891-1905(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, INDUCTION BY GONADOTROPIN.
[7]"Solution structure of the zinc finger, C4-type domain of human COUP transcription factor 1."
RIKEN structural genomics initiative (RSGI)
Submitted (FEB-2008) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 84-162.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X16155 mRNA. Translation: CAA34277.1.
X12795 Genomic DNA. Translation: CAA31283.1.
BC004154 mRNA. Translation: AAH04154.1.
BC017493 mRNA. Translation: AAH17493.1.
CCDSCCDS4068.1.
PIRS02710.
RefSeqNP_005645.1. NM_005654.5.
UniGeneHs.519445.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2EBLNMR-A84-159[»]
ProteinModelPortalP10589.
SMRP10589. Positions 82-414.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112883. 17 interactions.
DIPDIP-622N.
IntActP10589. 1 interaction.
MINTMINT-1514389.
STRING9606.ENSP00000325819.

Chemistry

ChEMBLCHEMBL1961789.

PTM databases

PhosphoSiteP10589.

Polymorphism databases

DMDM116959.

Proteomic databases

MaxQBP10589.
PaxDbP10589.
PRIDEP10589.

Protocols and materials databases

DNASU7025.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000327111; ENSP00000325819; ENSG00000175745.
GeneID7025.
KEGGhsa:7025.
UCSCuc003kkj.3. human.

Organism-specific databases

CTD7025.
GeneCardsGC05P092920.
HGNCHGNC:7975. NR2F1.
HPACAB019281.
MIM132890. gene.
neXtProtNX_P10589.
PharmGKBPA31758.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG327099.
HOGENOMHOG000260820.
HOVERGENHBG005606.
InParanoidP10589.
KOK08547.
OMADSAHIES.
OrthoDBEOG72RMZ5.
PhylomeDBP10589.
TreeFamTF352097.

Enzyme and pathway databases

ReactomeREACT_71. Gene Expression.
SignaLinkP10589.

Gene expression databases

BgeeP10589.
CleanExHS_NR2F1.
GenevestigatorP10589.

Family and domain databases

Gene3D1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProIPR003068. COUP_TF.
IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSPR01282. COUPTNFACTOR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMSSF48508. SSF48508. 1 hit.
PROSITEPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNR2F1. human.
EvolutionaryTraceP10589.
GeneWikiCOUP-TFI.
GenomeRNAi7025.
NextBio27447.
PROP10589.
SOURCESearch...

Entry information

Entry nameCOT1_HUMAN
AccessionPrimary (citable) accession number: P10589
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: July 9, 2014
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM