P10551 (THIL_SACBA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-CoA acetyltransferase
EC=2.3.1.9

Alternative name(s):
Acetoacetyl-CoA thiolase
Ergosterol biosynthesis protein 10

Gene names

Name:

ERG10

Organism

Saccharomyces bayanus (Yeast) (Saccharomyces uvarum)

Taxonomic identifier

4931 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of acetoacetyl-CoA in the biosynthesis of mevalonate, an intermediate required for the biosynthesis of sterols and nonsterol isoprenoids By similarity.
Catalytic activity	2 acetyl-CoA = CoA + acetoacetyl-CoA.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 1/3.
Subunit structure	Multimeric.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Potassium
Molecular function	Acyltransferase Transferase
PTM	Acetylation
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA C-acetyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 398	397	Acetyl-CoA acetyltransferase		PRO_0000206417
Sites
	Active site	91	1	Acyl-thioester intermediate By similarity
	Active site	354	1	Proton acceptor By similarity
	Active site	384	1	Proton acceptor By similarity
	Metal binding	186	1	Potassium By similarity
	Metal binding	248	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	249	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	251	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	350	1	Potassium; via carbonyl oxygen By similarity
	Binding site	186	1	Coenzyme A By similarity
	Binding site	231	1	Coenzyme A By similarity
	Binding site	252	1	Coenzyme A By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P10551 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: C0AC394C17A925AB	FASTA	398	41,659
	10 20 30 40 50 60 MSQNVYIVST ARTPIGSFQG SLSSKTAVEL GAAALKGALA KVPELDASKD FDEIIFGNVL 70 80 90 100 110 120 SANLGQAPAR QVALTAGLGN HIVATTVNKV CASAMKAIIL GAQSIKCGNA DVVVAGGCES 130 140 150 160 170 180 MTNAPYYMPA ARGGAKFGQT VLIDGVERDG LNDAYDGLAM GVHAEKCARD WDITRDQQDS 190 200 210 220 230 240 FAIESYQKSQ QSQKEGKFDN EIVPVTIKGF RGKPDTQVTN DEEPARLHVE KLKSARTVFQ 250 260 270 280 290 300 RENGTVTAAN ASPINDGAAA IILVSERVLK EKNLKPLAIV KGWGEAAHLP ADFTWAPSLA 310 320 330 340 350 360 VPKALKHAGI EDINSVDYFE FNEAFSVVGL VNTKILKLDP SKVNVYGGAV ALGHPLGCSG 370 380 390 ARVVVTLLSI LQQEGGKIGV AAICNGGGGA SSVVIEKL

References

[1]	"Cloning, sequencing and analysis of the yeast S. uvarum ERG10 gene encoding acetoacetyl CoA thiolase." Dequin S., Gloeckler R., Herbert C.J., Boutelet B. Curr. Genet. 13:471-478(1988) [PubMed: 2900076] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X07976 Genomic DNA. Translation: CAA30788.1.
3D structure databases
ProteinModelPortal	P10551.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002155. Thiolase. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. IPR020615. Thiolase_acyl_enz_int_AS. IPR020610. Thiolase_AS. IPR020617. Thiolase_C. IPR020613. Thiolase_CS. IPR020616. Thiolase_N. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 4 hits.
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THIL_SACBA

Accession

Primary (citable) accession number: P10551

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	September 21, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program