Reviewed, UniProtKB/Swiss-Prot P10548 (LSTP_STAST)

Names and origin

Protein names

Recommended name:
    Lysostaphin
    EC=3.4.24.75
Alternative name(s):
    Glycyl-glycine endopeptidase

Gene names

Name:

lss

Organism

Staphylococcus staphylolyticus

Taxonomic identifier

1287 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Lyses staphylococcal cells by hydrolyzing the polyglycine interpeptide bridges of the peptidoglycan.
Catalytic activity	Hydrolysis of the -Gly-|-Gly- bond in the pentaglycine inter-peptide link joining staphylococcal cell wall peptidoglycans.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase M23B family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Repeat Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Zymogen
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Propeptide	24 – 234	211			PRO_0000026817
	Chain	235 – 480	246	Lysostaphin		PRO_0000026818
Regions
	Repeat	49 – 61	13	1
	Repeat	62 – 74	13	2
	Repeat	75 – 87	13	3
	Repeat	88 – 100	13	4
	Repeat	101 – 113	13	5
	Repeat	114 – 126	13	6
	Repeat	127 – 139	13	7
	Repeat	140 – 152	13	8
	Repeat	153 – 165	13	9
	Repeat	166 – 178	13	10
	Repeat	179 – 191	13	11
	Repeat	192 – 204	13	12
	Repeat	205 – 217	13	13
	Repeat	218 – 230	13	14; approximate
	Region	49 – 230	182	14 X 13 AA tandem repeats of A-E-V-E-T-S-K-[AP]-P-V-E-N-T
Sites
	Active site	347	1	By similarity
	Metal binding	266	1	Zinc By similarity
	Metal binding	270	1	Zinc By similarity
	Metal binding	349	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	P10548-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 62A071A0D248891D	FASTA	480	51,687
	10 20 30 40 50 60 MKKTKNNYYT TPLAIGLSTF ALASIVYGGI QNETHASEKS NMDVSKKVAE VETSKPPVEN 70 80 90 100 110 120 TAEVETSKAP VENTAEVETS KAPVENTAEV ETSKAPVENT AEVETSKAPV ENTAEVETSK 130 140 150 160 170 180 APVENTAEVE TSKAPVENTA EVETSKAPVE NTAEVETSKA PVENTAEVET SKAPVENTAE 190 200 210 220 230 240 VETSKAPVEN TAEVETSKAP VENTAEVETS KAPVENTAEV ETSKALVQNR TALRAATHEH 250 260 270 280 290 300 SAQWLNNYKK GYGYGPYPLG INGGIHYGVD FFMNIGTPVK AISSGKIVEA GWSNYGGGNQ 310 320 330 340 350 360 IGLIENDGVH RQWYMHLSKY NVKVGDYVKA GQIIGWSGST GYSTAPHLHF QRMVNSFSNS 370 380 390 400 410 420 TAQDPMPFLK SAGYGKAGGT VTPTPNTGWK TNKYGTLYKS ESASFTPNTD IITRTTGPFR 430 440 450 460 470 480 SMPQSGVLKA GQTIHYDEVM KQDGHVWVGY TGNSGQRIYL PVRTWNKSTN TLGVLWGTIK

References

[1]	"The molecular organization of the lysostaphin gene and its sequences repeated in tandem." Heinrich P., Rosenstein R., Boehmer M., Sonner P., Goetz F. Mol. Gen. Genet. 209:563-569(1987) [PubMed: 2828883] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X06121 Genomic DNA. Translation: CAA29494.1.
PIR	S01079.
3D structure databases
SMR	P10548. Positions 389-480.
ModBase	Search...
Protein family/group databases
MEROPS	M23.004.
Enzyme and pathway databases
BRENDA	3.4.24.75. 19154.
Family and domain databases
InterPro	IPR016047. Peptidase_M23. IPR002886. Peptidase_M23B. IPR013667. SH3_5_bac. IPR003646. SH3_bac. [Graphical view]
PANTHER	PTHR21666:SF7. Peptidase_M23B. 1 hit.
Pfam	PF01551. Peptidase_M23. 1 hit. PF08460. SH3_5. 1 hit. [Graphical view]
SMART	SM00287. SH3b. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSTP_STAST

Accession

Primary (citable) accession number: P10548

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)