Reviewed, UniProtKB/Swiss-Prot P10547 (LSTP_STASI)

Names and origin

Protein names

Recommended name:
    Lysostaphin
    EC=3.4.24.75
Alternative name(s):
    Glycyl-glycine endopeptidase

Gene names

Name:

lss

Organism

Staphylococcus simulans

Taxonomic identifier

1286 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Lyses staphylococcal cells by hydrolyzing the polyglycine interpeptide bridges of the peptidoglycan.
Catalytic activity	Hydrolysis of the -Gly-|-Gly- bond in the pentaglycine inter-peptide link joining staphylococcal cell wall peptidoglycans.
Cofactor	Binds 1 zinc ion per subunit.
Subunit structure	Monomer.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase M23B family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Repeat Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Zymogen
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Propeptide	24 – 247	224			PRO_0000026815
	Chain	248 – 493	246	Lysostaphin		PRO_0000026816
Regions
	Repeat	49 – 61	13	1
	Repeat	62 – 74	13	2
	Repeat	75 – 87	13	3
	Repeat	88 – 100	13	4
	Repeat	101 – 113	13	5
	Repeat	114 – 126	13	6
	Repeat	127 – 139	13	7
	Repeat	140 – 152	13	8
	Repeat	153 – 165	13	9
	Repeat	166 – 178	13	10
	Repeat	179 – 191	13	11
	Repeat	192 – 204	13	12
	Repeat	205 – 217	13	13
	Repeat	218 – 230	13	14
	Repeat	231 – 243	13	15; approximate
	Region	49 – 243	195	15 X 13 AA approximate tandem repeats of A-E-V-E-T-S-K-A-P-V-E-N-T
Sites
	Active site	360	1
	Metal binding	279	1	Zinc
	Metal binding	283	1	Zinc
	Metal binding	362	1	Zinc
Experimental info
	Sequence conflict	135 – 238	104	Missing Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P10547-1 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: 2BFA751D47865A1A	FASTA	493	53,091
	10 20 30 40 50 60 MKKTKNNYYT RPLAIGLSTF ALASIVYGGI QNETHASEKS NMDVSKKVAE VETSKAPVEN 70 80 90 100 110 120 TAEVETSKAP VENTAEVETS KAPVENTAEV ETSKAPVENT AEVETSKAPV ENTAEVETSK 130 140 150 160 170 180 APVENTAEVE TSKAPVENTA EVETSKAPVE NTAEVETSKA PVENTAEVET SKAPVENTAE 190 200 210 220 230 240 VETSKAPVEN TAEVETSKAP VENTAEVETS KAPVENTAEV ETSKAPVENT AEVETSKALV 250 260 270 280 290 300 QNRTALRAAT HEHSAQWLNN YKKGYGYGPY PLGINGGMHY GVDFFMNIGT PVKAISSGKI 310 320 330 340 350 360 VEAGWSNYGG GNQIGLIEND GVHRQWYMHL SKYNVKVGDY VKAGQIIGWS GSTGYSTAPH 370 380 390 400 410 420 LHFQRMVNSF SNSTAQDPMP FLKSAGYGKA GGTVTPTPNT GWKTNKYGTL YKSESASFTP 430 440 450 460 470 480 NTDIITRTTG PFRSMPQSGV LKAGQTIHYD EVMKQDGHVW VGYTGNSGQR IYLPVRTWNK 490 STNTLGVLWG TIK

References

[1]	"Cloning, sequence, and expression of the lysostaphin gene from Staphylococcus simulans." Recsei P.A., Gruss A.D., Novick R.P. Proc. Natl. Acad. Sci. U.S.A. 84:1127-1131(1987) [PubMed: 3547405] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 20723 / NRRL B-2628.
[2]	"Studies on prolysostaphin processing and characterization of the lysostaphin immunity factor (Lif) of Staphylococcus simulans biovar staphylolyticus." Thumm G., Goetz F. Mol. Microbiol. 23:1251-1265(1997) [PubMed: 9106216] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 1362.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U66883 Genomic DNA. Translation: AAB53783.1. M15686 Genomic DNA. Translation: AAA26655.1.
PIR	A25881.
3D structure databases
SMR	P10547. Positions 402-493.
ModBase	Search...
Protein family/group databases
MEROPS	M23.004.
Enzyme and pathway databases
BRENDA	3.4.24.75. 19151.
Family and domain databases
InterPro	IPR016047. Peptidase_M23. IPR002886. Peptidase_M23B. IPR013667. SH3_5_bac. IPR003646. SH3_bac. [Graphical view]
PANTHER	PTHR21666:SF7. Peptidase_M23B. 1 hit.
Pfam	PF01551. Peptidase_M23. 1 hit. PF08460. SH3_5. 1 hit. [Graphical view]
SMART	SM00287. SH3b. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSTP_STASI

Accession

Primary (citable) accession number: P10547
Secondary accession number(s): O05988

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 15, 1998
Last modified:	June 16, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)