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Protein

Lysostaphin

Gene

lss

Organism
Staphylococcus simulans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lyses staphylococcal cells by hydrolyzing the polyglycine interpeptide bridges of the peptidoglycan.

Catalytic activityi

Hydrolysis of the -Gly-|-Gly- bond in the pentaglycine inter-peptide link joining staphylococcal cell wall peptidoglycans.

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi279Zinc1
Metal bindingi283Zinc1
Active sitei3601
Metal bindingi362Zinc1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Cell wall biogenesis/degradation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.24.75. 5881.

Protein family/group databases

MEROPSiM23.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysostaphin (EC:3.4.24.75)
Alternative name(s):
Glycyl-glycine endopeptidase
Gene namesi
Name:lss
OrganismiStaphylococcus simulans
Taxonomic identifieri1286 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
PropeptideiPRO_000002681524 – 247Add BLAST224
ChainiPRO_0000026816248 – 493LysostaphinAdd BLAST246

Keywords - PTMi

Zymogen

Proteomic databases

PRIDEiP10547.

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1493
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi251 – 253Combined sources3
Helixi256 – 260Combined sources5
Beta strandi267 – 269Combined sources3
Beta strandi271 – 273Combined sources3
Helixi274 – 276Combined sources3
Beta strandi281 – 285Combined sources5
Beta strandi291 – 293Combined sources3
Beta strandi298 – 306Combined sources9
Turni307 – 310Combined sources4
Beta strandi311 – 318Combined sources8
Beta strandi321 – 334Combined sources14
Beta strandi346 – 349Combined sources4
Beta strandi360 – 372Combined sources13
Helixi379 – 383Combined sources5
Turni385 – 388Combined sources4
Beta strandi389 – 391Combined sources3
Beta strandi399 – 401Combined sources3
Beta strandi410 – 422Combined sources13
Beta strandi424 – 431Combined sources8
Beta strandi437 – 441Combined sources5
Beta strandi447 – 455Combined sources9
Beta strandi458 – 464Combined sources7
Beta strandi470 – 479Combined sources10
Turni480 – 483Combined sources4
Beta strandi489 – 492Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LXCX-ray3.50A/B/C/D248-493[»]
4QP5X-ray1.26A/B248-386[»]
4QPBX-ray1.78A/B248-386[»]
ProteinModelPortaliP10547.
SMRiP10547.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati49 – 611Add BLAST13
Repeati62 – 742Add BLAST13
Repeati75 – 873Add BLAST13
Repeati88 – 1004Add BLAST13
Repeati101 – 1135Add BLAST13
Repeati114 – 1266Add BLAST13
Repeati127 – 1397Add BLAST13
Repeati140 – 1528Add BLAST13
Repeati153 – 1659Add BLAST13
Repeati166 – 17810Add BLAST13
Repeati179 – 19111Add BLAST13
Repeati192 – 20412Add BLAST13
Repeati205 – 21713Add BLAST13
Repeati218 – 23014Add BLAST13
Repeati231 – 24315; approximateAdd BLAST13
Domaini413 – 481SH3bPROSITE-ProRule annotationAdd BLAST69

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni49 – 24315 X 13 AA approximate tandem repeats of A-E-V-E-T-S-K-A-P-V-E-N-TAdd BLAST195

Sequence similaritiesi

Belongs to the peptidase M23B family.Curated
Contains 1 SH3b domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

KOiK08259.

Family and domain databases

InterProiIPR011055. Dup_hybrid_motif.
IPR016047. Peptidase_M23.
IPR003646. SH3-like_bac-type.
[Graphical view]
PfamiPF01551. Peptidase_M23. 1 hit.
PF08460. SH3_5. 1 hit.
[Graphical view]
SMARTiSM00287. SH3b. 1 hit.
[Graphical view]
SUPFAMiSSF51261. SSF51261. 1 hit.
PROSITEiPS51781. SH3B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10547-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKTKNNYYT RPLAIGLSTF ALASIVYGGI QNETHASEKS NMDVSKKVAE
60 70 80 90 100
VETSKAPVEN TAEVETSKAP VENTAEVETS KAPVENTAEV ETSKAPVENT
110 120 130 140 150
AEVETSKAPV ENTAEVETSK APVENTAEVE TSKAPVENTA EVETSKAPVE
160 170 180 190 200
NTAEVETSKA PVENTAEVET SKAPVENTAE VETSKAPVEN TAEVETSKAP
210 220 230 240 250
VENTAEVETS KAPVENTAEV ETSKAPVENT AEVETSKALV QNRTALRAAT
260 270 280 290 300
HEHSAQWLNN YKKGYGYGPY PLGINGGMHY GVDFFMNIGT PVKAISSGKI
310 320 330 340 350
VEAGWSNYGG GNQIGLIEND GVHRQWYMHL SKYNVKVGDY VKAGQIIGWS
360 370 380 390 400
GSTGYSTAPH LHFQRMVNSF SNSTAQDPMP FLKSAGYGKA GGTVTPTPNT
410 420 430 440 450
GWKTNKYGTL YKSESASFTP NTDIITRTTG PFRSMPQSGV LKAGQTIHYD
460 470 480 490
EVMKQDGHVW VGYTGNSGQR IYLPVRTWNK STNTLGVLWG TIK
Length:493
Mass (Da):53,091
Last modified:July 15, 1998 - v2
Checksum:i2BFA751D47865A1A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti135 – 238Missing (PubMed:3547405).CuratedAdd BLAST104

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66883 Genomic DNA. Translation: AAB53783.1.
M15686 Genomic DNA. Translation: AAA26655.1.
PIRiA25881.

Genome annotation databases

KEGGiag:AAA26655.
ag:AAB53783.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66883 Genomic DNA. Translation: AAB53783.1.
M15686 Genomic DNA. Translation: AAA26655.1.
PIRiA25881.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LXCX-ray3.50A/B/C/D248-493[»]
4QP5X-ray1.26A/B248-386[»]
4QPBX-ray1.78A/B248-386[»]
ProteinModelPortaliP10547.
SMRiP10547.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM23.004.

Proteomic databases

PRIDEiP10547.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAA26655.
ag:AAB53783.

Phylogenomic databases

KOiK08259.

Enzyme and pathway databases

BRENDAi3.4.24.75. 5881.

Family and domain databases

InterProiIPR011055. Dup_hybrid_motif.
IPR016047. Peptidase_M23.
IPR003646. SH3-like_bac-type.
[Graphical view]
PfamiPF01551. Peptidase_M23. 1 hit.
PF08460. SH3_5. 1 hit.
[Graphical view]
SMARTiSM00287. SH3b. 1 hit.
[Graphical view]
SUPFAMiSSF51261. SSF51261. 1 hit.
PROSITEiPS51781. SH3B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLSTP_STASI
AccessioniPrimary (citable) accession number: P10547
Secondary accession number(s): O05988
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 15, 1998
Last modified: November 2, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.