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Protein

Lysostaphin

Gene

lss

Organism
Staphylococcus simulans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lyses staphylococcal cells by hydrolyzing the polyglycine interpeptide bridges of the peptidoglycan.

Catalytic activityi

Hydrolysis of the -Gly-|-Gly- bond in the pentaglycine inter-peptide link joining staphylococcal cell wall peptidoglycans.

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi279 – 2791Zinc
Metal bindingi283 – 2831Zinc
Active sitei360 – 3601
Metal bindingi362 – 3621Zinc

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Cell wall biogenesis/degradation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.24.75. 5881.

Protein family/group databases

MEROPSiM23.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysostaphin (EC:3.4.24.75)
Alternative name(s):
Glycyl-glycine endopeptidase
Gene namesi
Name:lss
OrganismiStaphylococcus simulans
Taxonomic identifieri1286 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Propeptidei24 – 247224PRO_0000026815Add
BLAST
Chaini248 – 493246LysostaphinPRO_0000026816Add
BLAST

Keywords - PTMi

Zymogen

Proteomic databases

PRIDEiP10547.

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1
493
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi251 – 2533Combined sources
Helixi256 – 2605Combined sources
Beta strandi267 – 2693Combined sources
Beta strandi271 – 2733Combined sources
Helixi274 – 2763Combined sources
Beta strandi281 – 2855Combined sources
Beta strandi291 – 2933Combined sources
Beta strandi298 – 3069Combined sources
Turni307 – 3104Combined sources
Beta strandi311 – 3188Combined sources
Beta strandi321 – 33414Combined sources
Beta strandi346 – 3494Combined sources
Beta strandi360 – 37213Combined sources
Helixi379 – 3835Combined sources
Turni385 – 3884Combined sources
Beta strandi389 – 3913Combined sources
Beta strandi399 – 4013Combined sources
Beta strandi410 – 42213Combined sources
Beta strandi424 – 4318Combined sources
Beta strandi437 – 4415Combined sources
Beta strandi447 – 4559Combined sources
Beta strandi458 – 4647Combined sources
Beta strandi470 – 47910Combined sources
Turni480 – 4834Combined sources
Beta strandi489 – 4924Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4LXCX-ray3.50A/B/C/D248-493[»]
4QP5X-ray1.26A/B248-386[»]
4QPBX-ray1.78A/B248-386[»]
ProteinModelPortaliP10547.
SMRiP10547. Positions 402-493.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati49 – 61131Add
BLAST
Repeati62 – 74132Add
BLAST
Repeati75 – 87133Add
BLAST
Repeati88 – 100134Add
BLAST
Repeati101 – 113135Add
BLAST
Repeati114 – 126136Add
BLAST
Repeati127 – 139137Add
BLAST
Repeati140 – 152138Add
BLAST
Repeati153 – 165139Add
BLAST
Repeati166 – 1781310Add
BLAST
Repeati179 – 1911311Add
BLAST
Repeati192 – 2041312Add
BLAST
Repeati205 – 2171313Add
BLAST
Repeati218 – 2301314Add
BLAST
Repeati231 – 2431315; approximateAdd
BLAST
Domaini413 – 48169SH3bPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni49 – 24319515 X 13 AA approximate tandem repeats of A-E-V-E-T-S-K-A-P-V-E-N-TAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M23B family.Curated
Contains 1 SH3b domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

KOiK08259.

Family and domain databases

InterProiIPR011055. Dup_hybrid_motif.
IPR016047. Peptidase_M23.
IPR003646. SH3-like_bac-type.
[Graphical view]
PfamiPF01551. Peptidase_M23. 1 hit.
PF08460. SH3_5. 1 hit.
[Graphical view]
SMARTiSM00287. SH3b. 1 hit.
[Graphical view]
SUPFAMiSSF51261. SSF51261. 1 hit.
PROSITEiPS51781. SH3B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10547-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKTKNNYYT RPLAIGLSTF ALASIVYGGI QNETHASEKS NMDVSKKVAE
60 70 80 90 100
VETSKAPVEN TAEVETSKAP VENTAEVETS KAPVENTAEV ETSKAPVENT
110 120 130 140 150
AEVETSKAPV ENTAEVETSK APVENTAEVE TSKAPVENTA EVETSKAPVE
160 170 180 190 200
NTAEVETSKA PVENTAEVET SKAPVENTAE VETSKAPVEN TAEVETSKAP
210 220 230 240 250
VENTAEVETS KAPVENTAEV ETSKAPVENT AEVETSKALV QNRTALRAAT
260 270 280 290 300
HEHSAQWLNN YKKGYGYGPY PLGINGGMHY GVDFFMNIGT PVKAISSGKI
310 320 330 340 350
VEAGWSNYGG GNQIGLIEND GVHRQWYMHL SKYNVKVGDY VKAGQIIGWS
360 370 380 390 400
GSTGYSTAPH LHFQRMVNSF SNSTAQDPMP FLKSAGYGKA GGTVTPTPNT
410 420 430 440 450
GWKTNKYGTL YKSESASFTP NTDIITRTTG PFRSMPQSGV LKAGQTIHYD
460 470 480 490
EVMKQDGHVW VGYTGNSGQR IYLPVRTWNK STNTLGVLWG TIK
Length:493
Mass (Da):53,091
Last modified:July 15, 1998 - v2
Checksum:i2BFA751D47865A1A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti135 – 238104Missing (PubMed:3547405).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66883 Genomic DNA. Translation: AAB53783.1.
M15686 Genomic DNA. Translation: AAA26655.1.
PIRiA25881.

Genome annotation databases

KEGGiag:AAA26655.
ag:AAB53783.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66883 Genomic DNA. Translation: AAB53783.1.
M15686 Genomic DNA. Translation: AAA26655.1.
PIRiA25881.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4LXCX-ray3.50A/B/C/D248-493[»]
4QP5X-ray1.26A/B248-386[»]
4QPBX-ray1.78A/B248-386[»]
ProteinModelPortaliP10547.
SMRiP10547. Positions 402-493.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM23.004.

Proteomic databases

PRIDEiP10547.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAA26655.
ag:AAB53783.

Phylogenomic databases

KOiK08259.

Enzyme and pathway databases

BRENDAi3.4.24.75. 5881.

Family and domain databases

InterProiIPR011055. Dup_hybrid_motif.
IPR016047. Peptidase_M23.
IPR003646. SH3-like_bac-type.
[Graphical view]
PfamiPF01551. Peptidase_M23. 1 hit.
PF08460. SH3_5. 1 hit.
[Graphical view]
SMARTiSM00287. SH3b. 1 hit.
[Graphical view]
SUPFAMiSSF51261. SSF51261. 1 hit.
PROSITEiPS51781. SH3B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLSTP_STASI
AccessioniPrimary (citable) accession number: P10547
Secondary accession number(s): O05988
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 15, 1998
Last modified: June 8, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.