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P10536

- RAB1B_RAT

UniProt

P10536 - RAB1B_RAT

Protein

Ras-related protein Rab-1B

Gene

Rab1b

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Protein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi15 – 228GTPBy similarity
    Nucleotide bindingi63 – 675GTPBy similarity
    Nucleotide bindingi121 – 1244GTPBy similarity

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB

    GO - Biological processi

    1. protein transport Source: UniProtKB-KW
    2. small GTPase mediated signal transduction Source: InterPro

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras-related protein Rab-1B
    Gene namesi
    Name:Rab1b
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi1359415. MGC105830.

    Subcellular locationi

    Membrane 1 Publication; Lipid-anchor 1 Publication; Cytoplasmic side 1 Publication. Cytoplasm 1 Publication
    Note: Targeted by REP1 to membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus, and intermediate vesicles between these two compartments. In the GDP-form, colocalizes with GDI in the cytoplasm.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi21 – 211K → M: Abolishes GTP-binding. 1 Publication
    Mutagenesisi65 – 651A → T: Reduced GTPase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 201201Ras-related protein Rab-1BPRO_0000121063Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei76 – 761O-(2-cholinephosphoryl)serineBy similarity
    Lipidationi200 – 2001S-geranylgeranyl cysteine1 Publication
    Modified residuei201 – 2011Cysteine methyl esterSequence Analysis
    Lipidationi201 – 2011S-geranylgeranyl cysteine1 Publication

    Post-translational modificationi

    Prenylated; by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1).By similarity

    Keywords - PTMi

    Acetylation, Lipoprotein, Methylation, Phosphoprotein, Prenylation

    Proteomic databases

    PaxDbiP10536.
    PRIDEiP10536.

    PTM databases

    PhosphoSiteiP10536.

    Expressioni

    Gene expression databases

    GenevestigatoriP10536.

    Interactioni

    Subunit structurei

    Interacts with MICAL1, MICAL2 and MICAL3. Interacts with GDI1; the interaction requires the GDP-bound state. Interacts with CHM/REP1; the interaction requires the GDP-bound form and is necessary for prenylation by GGTase II By similarity.By similarity

    Protein-protein interaction databases

    IntActiP10536. 1 interaction.
    MINTiMINT-4577563.
    STRINGi10116.ENSRNOP00000027486.

    Structurei

    3D structure databases

    ProteinModelPortaliP10536.
    SMRiP10536. Positions 5-173.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni64 – 8320Switch 2 region; required for interaction with REP1/CHMBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi37 – 459Effector regionBy similarity

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000233968.
    HOVERGENiHBG009351.
    InParanoidiP10536.
    PhylomeDBiP10536.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00175. RAB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P10536-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNPEYDYLFK LLLIGDSGVG KSCLLLRFAD DTYTESYIST IGVDFKIRTI    50
    ELDGKTIKLQ IWDTAGQERF RTVTSSYYRG AHGIIVVYDV TDQESYANVK 100
    QWLQEIDRYA SENVNKLLVG NKSDLTTKKV VDNTTAKEFA DSLGVPFLET 150
    SAKNATNVEQ AFMTMAAEIK KRMGPGAASG GERPNLKIDS TPVKSASGGC 200
    C 201
    Length:201
    Mass (Da):22,163
    Last modified:July 1, 1989 - v1
    Checksum:i8D3EEDC2AEF4A2FE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13905 mRNA. Translation: CAA32105.1.
    PIRiS06147.
    UniGeneiRn.155100.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13905 mRNA. Translation: CAA32105.1 .
    PIRi S06147.
    UniGenei Rn.155100.

    3D structure databases

    ProteinModelPortali P10536.
    SMRi P10536. Positions 5-173.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P10536. 1 interaction.
    MINTi MINT-4577563.
    STRINGi 10116.ENSRNOP00000027486.

    PTM databases

    PhosphoSitei P10536.

    Proteomic databases

    PaxDbi P10536.
    PRIDEi P10536.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    RGDi 1359415. MGC105830.

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000233968.
    HOVERGENi HBG009351.
    InParanoidi P10536.
    PhylomeDBi P10536.

    Gene expression databases

    Genevestigatori P10536.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00175. RAB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of a rat cDNA: rab1B, encoding a rab1-YPT related protein."
      Zahraoui A., Touchot N., Chardin P., Tavitian A.
      Nucleic Acids Res. 17:1770-1770(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Biochemical properties of the YPT-related rab1B protein. Comparison with rab1A."
      Touchot N., Zahraoui A., Vielh E., Tavitian A.
      FEBS Lett. 256:79-84(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, MUTAGENESIS OF LYS-21 AND ALA-65.
    3. "Rab1b regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments."
      Plutner H., Cox A.D., Pind S., Khosravi-Far R., Bourne J.R., Schwaninger R., Der C.J., Balch W.E.
      J. Cell Biol. 115:31-43(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, FUNCTION.
    4. Cited for: ISOPRENYLATION AT CYS-200 AND CYS-201.

    Entry informationi

    Entry nameiRAB1B_RAT
    AccessioniPrimary (citable) accession number: P10536
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Rab-1B binds GTP and GDP and possesses intrinsic GTPase activity.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3