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Protein

Ras-related protein Rab-1B

Gene

Rab1b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Rab1B regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Plays a role in the initial events of the autophagic vacuole development which take place at specialized regions of the endoplasmic reticulum (By similarity).By similarity1 Publication

Enzyme regulationi

Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP).Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 23GTPBy similarity9
Nucleotide bindingi33 – 40GTPBy similarity8
Nucleotide bindingi63 – 67GTPBy similarity5
Nucleotide bindingi121 – 124GTPBy similarity4
Nucleotide bindingi151 – 153GTPBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-1B
Gene namesi
Name:Rab1b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1359415. MGC105830.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi21K → M: Abolishes GTP-binding. 1 Publication1
Mutagenesisi65A → T: Reduced GTPase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001210631 – 201Ras-related protein Rab-1BAdd BLAST201

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei76O-(2-cholinephosphoryl)serineBy similarity1
Lipidationi200S-geranylgeranyl cysteine1 Publication1
Modified residuei201Cysteine methyl esterSequence analysis1
Lipidationi201S-geranylgeranyl cysteine1 Publication1

Post-translational modificationi

Prenylated; by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1).By similarity

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiP10536.
PRIDEiP10536.

PTM databases

iPTMnetiP10536.
PhosphoSitePlusiP10536.
SwissPalmiP10536.

Interactioni

Subunit structurei

Interacts with MICAL1, MICAL2 and MICAL3. Interacts with GDI1; the interaction requires the GDP-bound state. Interacts with CHM/REP1; the interaction requires the GDP-bound form and is necessary for prenylation by GGTase II.By similarity

Protein-protein interaction databases

IntActiP10536. 1 interactor.
MINTiMINT-4577563.
STRINGi10116.ENSRNOP00000067788.

Structurei

3D structure databases

ProteinModelPortaliP10536.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni64 – 83Switch 2 region; required for interaction with REP1/CHMBy similarityAdd BLAST20

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi37 – 45Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0084. Eukaryota.
ENOG410XQN5. LUCA.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP10536.
PhylomeDBiP10536.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10536-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPEYDYLFK LLLIGDSGVG KSCLLLRFAD DTYTESYIST IGVDFKIRTI
60 70 80 90 100
ELDGKTIKLQ IWDTAGQERF RTVTSSYYRG AHGIIVVYDV TDQESYANVK
110 120 130 140 150
QWLQEIDRYA SENVNKLLVG NKSDLTTKKV VDNTTAKEFA DSLGVPFLET
160 170 180 190 200
SAKNATNVEQ AFMTMAAEIK KRMGPGAASG GERPNLKIDS TPVKSASGGC

C
Length:201
Mass (Da):22,163
Last modified:July 1, 1989 - v1
Checksum:i8D3EEDC2AEF4A2FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13905 mRNA. Translation: CAA32105.1.
PIRiS06147.
UniGeneiRn.155100.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13905 mRNA. Translation: CAA32105.1.
PIRiS06147.
UniGeneiRn.155100.

3D structure databases

ProteinModelPortaliP10536.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP10536. 1 interactor.
MINTiMINT-4577563.
STRINGi10116.ENSRNOP00000067788.

PTM databases

iPTMnetiP10536.
PhosphoSitePlusiP10536.
SwissPalmiP10536.

Proteomic databases

PaxDbiP10536.
PRIDEiP10536.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

RGDi1359415. MGC105830.

Phylogenomic databases

eggNOGiKOG0084. Eukaryota.
ENOG410XQN5. LUCA.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP10536.
PhylomeDBiP10536.

Miscellaneous databases

PROiP10536.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAB1B_RAT
AccessioniPrimary (citable) accession number: P10536
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Rab-1B binds GTP and GDP and possesses intrinsic GTPase activity.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.