P10536 (RAB1B_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-1B

Gene names

Name:

Rab1b

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Ref.3
Subunit structure	Interacts with MICAL1, MICAL2 and MICAL3. Interacts with GDI1; the interaction requires the GDP-bound state. Interacts with CHM/REP1; the interaction requires the GDP-bound form and is necessary for prenylation by GGTase II By similarity.
Subcellular location	Membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm. Note: Targeted by REP1 to membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus, and intermediate vesicles between these two compartments. In the GDP-form, colocalizes with GDI in the cytoplasm. Ref.3
Post-translational modification	Prenylated; by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1) By similarity.
Miscellaneous	Rab-1B binds GTP and GDP and possesses intrinsic GTPase activity.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Acetylation Lipoprotein Methylation Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 201	201	Ras-related protein Rab-1B		PRO_0000121063
Regions
	Nucleotide binding	15 – 22	8	GTP By similarity
	Nucleotide binding	63 – 67	5	GTP By similarity
	Nucleotide binding	121 – 124	4	GTP By similarity
	Region	64 – 83	20	Switch 2 region; required for interaction with REP1/CHM By similarity
	Motif	37 – 45	9	Effector region By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	201	1	Cysteine methyl ester Potential
	Lipidation	200	1	S-geranylgeranyl cysteine Ref.4
	Lipidation	201	1	S-geranylgeranyl cysteine Ref.4
Experimental info
	Mutagenesis	21	1	K → M: Abolishes GTP-binding. Ref.2
	Mutagenesis	65	1	A → T: Reduced GTPase activity. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P10536 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 8D3EEDC2AEF4A2FE	FASTA	201	22,163
	10 20 30 40 50 60 MNPEYDYLFK LLLIGDSGVG KSCLLLRFAD DTYTESYIST IGVDFKIRTI ELDGKTIKLQ 70 80 90 100 110 120 IWDTAGQERF RTVTSSYYRG AHGIIVVYDV TDQESYANVK QWLQEIDRYA SENVNKLLVG 130 140 150 160 170 180 NKSDLTTKKV VDNTTAKEFA DSLGVPFLET SAKNATNVEQ AFMTMAAEIK KRMGPGAASG 190 200 GERPNLKIDS TPVKSASGGC C

References

[1]	"Nucleotide sequence of a rat cDNA: rab1B, encoding a rab1-YPT related protein." Zahraoui A., Touchot N., Chardin P., Tavitian A. Nucleic Acids Res. 17:1770-1770(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Biochemical properties of the YPT-related rab1B protein. Comparison with rab1A." Touchot N., Zahraoui A., Vielh E., Tavitian A. FEBS Lett. 256:79-84(1989) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, MUTAGENESIS OF LYS-21 AND ALA-65.
[3]	"Rab1b regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments." Plutner H., Cox A.D., Pind S., Khosravi-Far R., Bourne J.R., Schwaninger R., Der C.J., Balch W.E. J. Cell Biol. 115:31-43(1991) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, FUNCTION.
[4]	"Isoprenoid modification of rab proteins terminating in CC or CXC motifs." Khosravi-Far R., Lutz R.J., Cox A.D., Conroy L., Bourne J.R., Sinensky M., Balch W.E., Buss J.E., Der C.J. Proc. Natl. Acad. Sci. U.S.A. 88:6264-6268(1991) [PubMed] [Europe PMC] [Abstract] Cited for: ISOPRENYLATION AT CYS-200 AND CYS-201.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X13905 mRNA. Translation: CAA32105.1.
IPI	IPI00480673.
PIR	S06147.
UniGene	Rn.155100.
3D structure databases
ProteinModelPortal	P10536.
SMR	P10536. Positions 5-173.
ModBase	Search...
Protein-protein interaction databases
IntAct	P10536. 1 interaction.
MINT	MINT-4577563.
STRING	10116.ENSRNOP00000027486.
PTM databases
PhosphoSite	P10536.
Proteomic databases
PaxDb	P10536.
PRIDE	P10536.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
RGD	1359415. MGC105830.
Phylogenomic databases
eggNOG	COG1100.
HOGENOM	HOG000233968.
HOVERGEN	HBG009351.
InParanoid	P10536.
OrthoDB	EOG48D0WM.
Gene expression databases
Genevestigator	P10536.
GermOnline	ENSRNOG00000003753. Rattus norvegicus. ENSRNOG00000020253. Rattus norvegicus.
Family and domain databases
InterPro	IPR027417. P-loop_NTPase. IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003579. Small_GTPase_Rab_type. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00175. RAB. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51419. RAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RAB1B_RAT

Accession

Primary (citable) accession number: P10536

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 29, 2013
This is version 117 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program