Reviewed, UniProtKB/Swiss-Prot P10509 (BLAC_STRAU)

Names and origin

Protein names

Recommended name:
    Beta-lactamase
    EC=3.5.2.6
Alternative name(s):
    Penicillinase

Gene names

Name:

bla

Organism

Streptomyces aureofaciens

Taxonomic identifier

1894 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Tat-type signal Potential
	Chain	35 – 311	277	Beta-lactamase		PRO_0000017013
Regions
	Region	255 – 257	3	Substrate binding By similarity
Sites
	Active site	87	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P10509-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 6FC8F4E5CD4C1DFA	FASTA	311	32,486
	10 20 30 40 50 60 MRLTQAPPSR RTLMTLGAGA TMAALLPAGG AAYASTSTAK APAAEGISGR LRALEKQYAA 70 80 90 100 110 120 RLGVFALDTG TGAGRSYRAG ERFPMCSVFK ALAAAAVLRD VDARREFLTK RIHYTEKFVK 130 140 150 160 170 180 DAGYIPVTGK PENIAGGMTG AELCAAAVSE SDNGAGNLLL RELDGPTGIT RFCRSLGDTT 190 200 210 220 230 240 TRLDRWEPAL NSAEPDRVTD TTSPGAIGRT FGRLIVGSAL RAGDRKRLTG WLVANTTNRP 250 260 270 280 290 300 TFRAGLPDDW VLADKTGGGE QYGVANDVGV VQPPGRAPLV LSVLSTKFDP KGPTDNPLVA 310 KAAALVAGEL T

References

[1]	Reynes J.-P., Drocourt D., Tiraby G. Submitted (NOV-1988) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X13597 Genomic DNA. Translation: CAA31933.1.
PIR	S02714.
3D structure databases
HSSP	HSSP built from PDB template 1BSG based on UniProtKB P14559.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.5.2.6. 2126.
Family and domain databases
InterPro	IPR001466. Beta_lactamase-related. IPR000871. Beta_lactamase_A/D. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLAC_STRAU

Accession

Primary (citable) accession number: P10509

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)