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P10507

- MPPB_YEAST

UniProt

P10507 - MPPB_YEAST

Protein

Mitochondrial-processing peptidase subunit beta

Gene

MAS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 151 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Cleaves presequences (transit peptides) from mitochondrial protein precursors.

    Catalytic activityi

    Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.

    Cofactori

    Binds 1 zinc ion per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi70 – 701Zinc1 PublicationPROSITE-ProRule annotation
    Active sitei73 – 731Proton acceptor
    Metal bindingi74 – 741Zinc1 PublicationPROSITE-ProRule annotation
    Metal bindingi150 – 1501Zinc1 PublicationPROSITE-ProRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. metalloendopeptidase activity Source: Reactome
    3. protein binding Source: IntAct

    GO - Biological processi

    1. protein processing involved in protein targeting to mitochondrion Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16673.
    YEAST:G3O-32293-MONOMER.
    ReactomeiREACT_189012. Mitochondrial protein import.
    SABIO-RKP10507.

    Protein family/group databases

    MEROPSiM16.003.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial-processing peptidase subunit beta (EC:3.4.24.64)
    Alternative name(s):
    Beta-MPP
    PEP
    Gene namesi
    Name:MAS1
    Synonyms:MIF1
    Ordered Locus Names:YLR163C
    ORF Names:L9632.10
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR163c.
    SGDiS000004153. MAS1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial matrix Source: Reactome
    2. mitochondrial processing peptidase complex Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi73 – 731E → Q: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2020Mitochondrion1 PublicationAdd
    BLAST
    Chaini21 – 462442Mitochondrial-processing peptidase subunit betaPRO_0000026784Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei243 – 2431Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP10507.
    PaxDbiP10507.
    PeptideAtlasiP10507.
    PRIDEiP10507.

    Expressioni

    Gene expression databases

    GenevestigatoriP10507.

    Interactioni

    Subunit structurei

    Heterodimer of alpha and beta subunits.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MAS2P119145EBI-11212,EBI-11205

    Protein-protein interaction databases

    BioGridi31436. 20 interactions.
    DIPiDIP-2402N.
    IntActiP10507. 14 interactions.
    MINTiMINT-614379.
    STRINGi4932.YLR163C.

    Structurei

    Secondary structure

    1
    462
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi27 – 304
    Beta strandi36 – 416
    Beta strandi46 – 5510
    Helixi58 – 603
    Turni63 – 675
    Helixi68 – 758
    Beta strandi78 – 825
    Helixi85 – 9410
    Beta strandi98 – 1036
    Beta strandi105 – 11511
    Helixi116 – 1183
    Helixi119 – 13113
    Helixi137 – 15418
    Helixi158 – 17013
    Turni171 – 1733
    Helixi175 – 1773
    Helixi184 – 1896
    Helixi192 – 20211
    Helixi205 – 2073
    Beta strandi208 – 2158
    Helixi218 – 22912
    Beta strandi254 – 2596
    Beta strandi264 – 27310
    Helixi282 – 29211
    Beta strandi294 – 2963
    Turni297 – 2993
    Beta strandi302 – 3054
    Helixi308 – 3147
    Beta strandi321 – 3299
    Beta strandi334 – 34310
    Turni344 – 3463
    Helixi349 – 36416
    Helixi370 – 38516
    Helixi391 – 40515
    Helixi411 – 4199
    Helixi423 – 43311
    Beta strandi434 – 4363
    Beta strandi439 – 4457
    Helixi447 – 4493
    Helixi453 – 4619

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1HR6X-ray2.50B/D/F/H21-462[»]
    1HR7X-ray2.55B/D/F/H21-462[»]
    1HR8X-ray2.70B/D/F/H21-462[»]
    1HR9X-ray3.01B/D/F/H21-462[»]
    ProteinModelPortaliP10507.
    SMRiP10507. Positions 23-462.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP10507.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M16 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0612.
    GeneTreeiENSGT00550000074701.
    HOGENOMiHOG000242450.
    KOiK17732.
    OMAiIWDQDIA.
    OrthoDBiEOG7BW0TR.

    Family and domain databases

    Gene3Di3.30.830.10. 2 hits.
    InterProiIPR011249. Metalloenz_LuxS/M16.
    IPR011237. Pept_M16_dom.
    IPR011765. Pept_M16_N.
    IPR001431. Pept_M16_Zn_BS.
    IPR007863. Peptidase_M16_C.
    [Graphical view]
    PfamiPF00675. Peptidase_M16. 1 hit.
    PF05193. Peptidase_M16_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF63411. SSF63411. 2 hits.
    PROSITEiPS00143. INSULINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P10507-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFSRTASKFR NTRRLLSTIS SQIPGTRTSK LPNGLTIATE YIPNTSSATV    50
    GIFVDAGSRA ENVKNNGTAH FLEHLAFKGT QNRSQQGIEL EIENIGSHLN 100
    AYTSRENTVY YAKSLQEDIP KAVDILSDIL TKSVLDNSAI ERERDVIIRE 150
    SEEVDKMYDE VVFDHLHEIT YKDQPLGRTI LGPIKNIKSI TRTDLKDYIT 200
    KNYKGDRMVL AGAGAVDHEK LVQYAQKYFG HVPKSESPVP LGSPRGPLPV 250
    FCRGERFIKE NTLPTTHIAI ALEGVSWSAP DYFVALATQA IVGNWDRAIG 300
    TGTNSPSPLA VAASQNGSLA NSYMSFSTSY ADSGLWGMYI VTDSNEHNVQ 350
    LIVNEILKEW KRIKSGKISD AEVNRAKAQL KAALLLSLDG STAIVEDIGR 400
    QVVTTGKRLS PEEVFEQVDK ITKDDIIMWA NYRLQNKPVS MVALGNTSTV 450
    PNVSYIEEKL NQ 462
    Length:462
    Mass (Da):51,084
    Last modified:July 1, 1989 - v1
    Checksum:i084CE63C495EDFC4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07649 Genomic DNA. Translation: CAA30489.1.
    U51921 Genomic DNA. Translation: AAB67487.1.
    AY693198 Genomic DNA. Translation: AAT93217.1.
    BK006945 Genomic DNA. Translation: DAA09485.1.
    PIRiS00552.
    RefSeqiNP_013264.1. NM_001182050.1.

    Genome annotation databases

    EnsemblFungiiYLR163C; YLR163C; YLR163C.
    GeneIDi850860.
    KEGGisce:YLR163C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07649 Genomic DNA. Translation: CAA30489.1 .
    U51921 Genomic DNA. Translation: AAB67487.1 .
    AY693198 Genomic DNA. Translation: AAT93217.1 .
    BK006945 Genomic DNA. Translation: DAA09485.1 .
    PIRi S00552.
    RefSeqi NP_013264.1. NM_001182050.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1HR6 X-ray 2.50 B/D/F/H 21-462 [» ]
    1HR7 X-ray 2.55 B/D/F/H 21-462 [» ]
    1HR8 X-ray 2.70 B/D/F/H 21-462 [» ]
    1HR9 X-ray 3.01 B/D/F/H 21-462 [» ]
    ProteinModelPortali P10507.
    SMRi P10507. Positions 23-462.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31436. 20 interactions.
    DIPi DIP-2402N.
    IntActi P10507. 14 interactions.
    MINTi MINT-614379.
    STRINGi 4932.YLR163C.

    Protein family/group databases

    MEROPSi M16.003.

    Proteomic databases

    MaxQBi P10507.
    PaxDbi P10507.
    PeptideAtlasi P10507.
    PRIDEi P10507.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR163C ; YLR163C ; YLR163C .
    GeneIDi 850860.
    KEGGi sce:YLR163C.

    Organism-specific databases

    CYGDi YLR163c.
    SGDi S000004153. MAS1.

    Phylogenomic databases

    eggNOGi COG0612.
    GeneTreei ENSGT00550000074701.
    HOGENOMi HOG000242450.
    KOi K17732.
    OMAi IWDQDIA.
    OrthoDBi EOG7BW0TR.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-16673.
    YEAST:G3O-32293-MONOMER.
    Reactomei REACT_189012. Mitochondrial protein import.
    SABIO-RK P10507.

    Miscellaneous databases

    EvolutionaryTracei P10507.
    NextBioi 967179.
    PROi P10507.

    Gene expression databases

    Genevestigatori P10507.

    Family and domain databases

    Gene3Di 3.30.830.10. 2 hits.
    InterProi IPR011249. Metalloenz_LuxS/M16.
    IPR011237. Pept_M16_dom.
    IPR011765. Pept_M16_N.
    IPR001431. Pept_M16_Zn_BS.
    IPR007863. Peptidase_M16_C.
    [Graphical view ]
    Pfami PF00675. Peptidase_M16. 1 hit.
    PF05193. Peptidase_M16_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF63411. SSF63411. 2 hits.
    PROSITEi PS00143. INSULINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "MAS1, a gene essential for yeast mitochondrial assembly, encodes a subunit of the mitochondrial processing protease."
      Witte C., Jensen R.E., Yaffe M.P., Schatz G.
      EMBO J. 7:1439-1447(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "The MAS-encoded processing protease of yeast mitochondria. Interaction of the purified enzyme with signal peptides and a purified precursor protein."
      Yang M., Geli V., Oppliger W., Suda K., James P., Schatz G.
      J. Biol. Chem. 266:6416-6423(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 21-32.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-243, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Crystal structures of mitochondrial processing peptidase reveal the mode for specific cleavage of import signal sequences."
      Taylor A.B., Smith B.S., Kitada S., Kojima K., Miyaura H., Otwinowski Z., Ito A., Deisenhofer J.
      Structure 9:615-625(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 21-462 IN COMPLEX WITH ZINC AND SUBSTRATES, MUTAGENESIS OF GLU-73.

    Entry informationi

    Entry nameiMPPB_YEAST
    AccessioniPrimary (citable) accession number: P10507
    Secondary accession number(s): D6VYG9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 151 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1360 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3