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P10507

- MPPB_YEAST

UniProt

P10507 - MPPB_YEAST

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Protein
Mitochondrial-processing peptidase subunit beta
Gene
MAS1, MIF1, YLR163C, L9632.10
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cleaves presequences (transit peptides) from mitochondrial protein precursors.

Catalytic activityi

Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.

Cofactori

Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Zinc
Active sitei73 – 731Proton acceptor
Metal bindingi74 – 741Zinc
Metal bindingi150 – 1501Zinc

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: Reactome
  3. protein binding Source: IntAct

GO - Biological processi

  1. protein processing involved in protein targeting to mitochondrion Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16673.
YEAST:G3O-32293-MONOMER.
ReactomeiREACT_189012. Mitochondrial protein import.
SABIO-RKP10507.

Protein family/group databases

MEROPSiM16.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial-processing peptidase subunit beta (EC:3.4.24.64)
Alternative name(s):
Beta-MPP
PEP
Gene namesi
Name:MAS1
Synonyms:MIF1
Ordered Locus Names:YLR163C
ORF Names:L9632.10
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR163c.
SGDiS000004153. MAS1.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial matrix Source: Reactome
  2. mitochondrial processing peptidase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi73 – 731E → Q: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2020Mitochondrion1 Publication
Add
BLAST
Chaini21 – 462442Mitochondrial-processing peptidase subunit beta
PRO_0000026784Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei243 – 2431Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP10507.
PaxDbiP10507.
PeptideAtlasiP10507.
PRIDEiP10507.

Expressioni

Gene expression databases

GenevestigatoriP10507.

Interactioni

Subunit structurei

Heterodimer of alpha and beta subunits.

Binary interactionsi

WithEntry#Exp.IntActNotes
MAS2P119145EBI-11212,EBI-11205

Protein-protein interaction databases

BioGridi31436. 20 interactions.
DIPiDIP-2402N.
IntActiP10507. 14 interactions.
MINTiMINT-614379.
STRINGi4932.YLR163C.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi27 – 304
Beta strandi36 – 416
Beta strandi46 – 5510
Helixi58 – 603
Turni63 – 675
Helixi68 – 758
Beta strandi78 – 825
Helixi85 – 9410
Beta strandi98 – 1036
Beta strandi105 – 11511
Helixi116 – 1183
Helixi119 – 13113
Helixi137 – 15418
Helixi158 – 17013
Turni171 – 1733
Helixi175 – 1773
Helixi184 – 1896
Helixi192 – 20211
Helixi205 – 2073
Beta strandi208 – 2158
Helixi218 – 22912
Beta strandi254 – 2596
Beta strandi264 – 27310
Helixi282 – 29211
Beta strandi294 – 2963
Turni297 – 2993
Beta strandi302 – 3054
Helixi308 – 3147
Beta strandi321 – 3299
Beta strandi334 – 34310
Turni344 – 3463
Helixi349 – 36416
Helixi370 – 38516
Helixi391 – 40515
Helixi411 – 4199
Helixi423 – 43311
Beta strandi434 – 4363
Beta strandi439 – 4457
Helixi447 – 4493
Helixi453 – 4619

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HR6X-ray2.50B/D/F/H21-462[»]
1HR7X-ray2.55B/D/F/H21-462[»]
1HR8X-ray2.70B/D/F/H21-462[»]
1HR9X-ray3.01B/D/F/H21-462[»]
ProteinModelPortaliP10507.
SMRiP10507. Positions 23-462.

Miscellaneous databases

EvolutionaryTraceiP10507.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M16 family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0612.
GeneTreeiENSGT00550000074701.
HOGENOMiHOG000242450.
KOiK17732.
OMAiIWDQDIA.
OrthoDBiEOG7BW0TR.

Family and domain databases

Gene3Di3.30.830.10. 2 hits.
InterProiIPR011249. Metalloenz_LuxS/M16.
IPR011237. Pept_M16_dom.
IPR011765. Pept_M16_N.
IPR001431. Pept_M16_Zn_BS.
IPR007863. Peptidase_M16_C.
[Graphical view]
PfamiPF00675. Peptidase_M16. 1 hit.
PF05193. Peptidase_M16_C. 1 hit.
[Graphical view]
SUPFAMiSSF63411. SSF63411. 2 hits.
PROSITEiPS00143. INSULINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10507-1 [UniParc]FASTAAdd to Basket

« Hide

MFSRTASKFR NTRRLLSTIS SQIPGTRTSK LPNGLTIATE YIPNTSSATV    50
GIFVDAGSRA ENVKNNGTAH FLEHLAFKGT QNRSQQGIEL EIENIGSHLN 100
AYTSRENTVY YAKSLQEDIP KAVDILSDIL TKSVLDNSAI ERERDVIIRE 150
SEEVDKMYDE VVFDHLHEIT YKDQPLGRTI LGPIKNIKSI TRTDLKDYIT 200
KNYKGDRMVL AGAGAVDHEK LVQYAQKYFG HVPKSESPVP LGSPRGPLPV 250
FCRGERFIKE NTLPTTHIAI ALEGVSWSAP DYFVALATQA IVGNWDRAIG 300
TGTNSPSPLA VAASQNGSLA NSYMSFSTSY ADSGLWGMYI VTDSNEHNVQ 350
LIVNEILKEW KRIKSGKISD AEVNRAKAQL KAALLLSLDG STAIVEDIGR 400
QVVTTGKRLS PEEVFEQVDK ITKDDIIMWA NYRLQNKPVS MVALGNTSTV 450
PNVSYIEEKL NQ 462
Length:462
Mass (Da):51,084
Last modified:July 1, 1989 - v1
Checksum:i084CE63C495EDFC4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X07649 Genomic DNA. Translation: CAA30489.1.
U51921 Genomic DNA. Translation: AAB67487.1.
AY693198 Genomic DNA. Translation: AAT93217.1.
BK006945 Genomic DNA. Translation: DAA09485.1.
PIRiS00552.
RefSeqiNP_013264.1. NM_001182050.1.

Genome annotation databases

EnsemblFungiiYLR163C; YLR163C; YLR163C.
GeneIDi850860.
KEGGisce:YLR163C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X07649 Genomic DNA. Translation: CAA30489.1 .
U51921 Genomic DNA. Translation: AAB67487.1 .
AY693198 Genomic DNA. Translation: AAT93217.1 .
BK006945 Genomic DNA. Translation: DAA09485.1 .
PIRi S00552.
RefSeqi NP_013264.1. NM_001182050.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1HR6 X-ray 2.50 B/D/F/H 21-462 [» ]
1HR7 X-ray 2.55 B/D/F/H 21-462 [» ]
1HR8 X-ray 2.70 B/D/F/H 21-462 [» ]
1HR9 X-ray 3.01 B/D/F/H 21-462 [» ]
ProteinModelPortali P10507.
SMRi P10507. Positions 23-462.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31436. 20 interactions.
DIPi DIP-2402N.
IntActi P10507. 14 interactions.
MINTi MINT-614379.
STRINGi 4932.YLR163C.

Protein family/group databases

MEROPSi M16.003.

Proteomic databases

MaxQBi P10507.
PaxDbi P10507.
PeptideAtlasi P10507.
PRIDEi P10507.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR163C ; YLR163C ; YLR163C .
GeneIDi 850860.
KEGGi sce:YLR163C.

Organism-specific databases

CYGDi YLR163c.
SGDi S000004153. MAS1.

Phylogenomic databases

eggNOGi COG0612.
GeneTreei ENSGT00550000074701.
HOGENOMi HOG000242450.
KOi K17732.
OMAi IWDQDIA.
OrthoDBi EOG7BW0TR.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-16673.
YEAST:G3O-32293-MONOMER.
Reactomei REACT_189012. Mitochondrial protein import.
SABIO-RK P10507.

Miscellaneous databases

EvolutionaryTracei P10507.
NextBioi 967179.
PROi P10507.

Gene expression databases

Genevestigatori P10507.

Family and domain databases

Gene3Di 3.30.830.10. 2 hits.
InterProi IPR011249. Metalloenz_LuxS/M16.
IPR011237. Pept_M16_dom.
IPR011765. Pept_M16_N.
IPR001431. Pept_M16_Zn_BS.
IPR007863. Peptidase_M16_C.
[Graphical view ]
Pfami PF00675. Peptidase_M16. 1 hit.
PF05193. Peptidase_M16_C. 1 hit.
[Graphical view ]
SUPFAMi SSF63411. SSF63411. 2 hits.
PROSITEi PS00143. INSULINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "MAS1, a gene essential for yeast mitochondrial assembly, encodes a subunit of the mitochondrial processing protease."
    Witte C., Jensen R.E., Yaffe M.P., Schatz G.
    EMBO J. 7:1439-1447(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "The MAS-encoded processing protease of yeast mitochondria. Interaction of the purified enzyme with signal peptides and a purified precursor protein."
    Yang M., Geli V., Oppliger W., Suda K., James P., Schatz G.
    J. Biol. Chem. 266:6416-6423(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-32.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-243, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Crystal structures of mitochondrial processing peptidase reveal the mode for specific cleavage of import signal sequences."
    Taylor A.B., Smith B.S., Kitada S., Kojima K., Miyaura H., Otwinowski Z., Ito A., Deisenhofer J.
    Structure 9:615-625(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 21-462 IN COMPLEX WITH ZINC AND SUBSTRATES, MUTAGENESIS OF GLU-73.

Entry informationi

Entry nameiMPPB_YEAST
AccessioniPrimary (citable) accession number: P10507
Secondary accession number(s): D6VYG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: September 3, 2014
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1360 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

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