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Protein

Mitochondrial-processing peptidase subunit beta

Gene

MAS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves presequences (transit peptides) from mitochondrial protein precursors.

Miscellaneous

Present with 1360 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi70ZincPROSITE-ProRule annotation1 Publication1
Active sitei73Proton acceptor1
Metal bindingi74ZincPROSITE-ProRule annotation1 Publication1
Metal bindingi150ZincPROSITE-ProRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:G3O-32293-MONOMER
YEAST:G3O-32293-MONOMER
SABIO-RKiP10507

Protein family/group databases

MEROPSiM16.003

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial-processing peptidase subunit beta (EC:3.4.24.64)
Alternative name(s):
Beta-MPP
PEP
Gene namesi
Name:MAS1
Synonyms:MIF1
Ordered Locus Names:YLR163C
ORF Names:L9632.10
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi

Organism-specific databases

EuPathDBiFungiDB:YLR163C
SGDiS000004153 MAS1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi73E → Q: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 20Mitochondrion1 PublicationAdd BLAST20
ChainiPRO_000002678421 – 462Mitochondrial-processing peptidase subunit betaAdd BLAST442

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei243PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP10507
PaxDbiP10507
PRIDEiP10507

PTM databases

iPTMnetiP10507

Interactioni

Subunit structurei

Heterodimer of alpha and beta subunits.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MAS2P119144EBI-11212,EBI-11205

Protein-protein interaction databases

BioGridi31436, 87 interactors
DIPiDIP-2402N
IntActiP10507, 16 interactors
MINTiP10507
STRINGi4932.YLR163C

Structurei

Secondary structure

1462
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi27 – 30Combined sources4
Beta strandi36 – 41Combined sources6
Beta strandi46 – 55Combined sources10
Helixi58 – 60Combined sources3
Turni63 – 67Combined sources5
Helixi68 – 75Combined sources8
Beta strandi78 – 82Combined sources5
Helixi85 – 94Combined sources10
Beta strandi98 – 103Combined sources6
Beta strandi105 – 115Combined sources11
Helixi116 – 118Combined sources3
Helixi119 – 131Combined sources13
Helixi137 – 154Combined sources18
Helixi158 – 170Combined sources13
Turni171 – 173Combined sources3
Helixi175 – 177Combined sources3
Helixi184 – 189Combined sources6
Helixi192 – 202Combined sources11
Helixi205 – 207Combined sources3
Beta strandi208 – 215Combined sources8
Helixi218 – 229Combined sources12
Beta strandi254 – 259Combined sources6
Beta strandi264 – 273Combined sources10
Helixi282 – 292Combined sources11
Beta strandi294 – 296Combined sources3
Turni297 – 299Combined sources3
Beta strandi302 – 305Combined sources4
Helixi308 – 314Combined sources7
Beta strandi321 – 329Combined sources9
Beta strandi334 – 343Combined sources10
Turni344 – 346Combined sources3
Helixi349 – 364Combined sources16
Helixi370 – 385Combined sources16
Helixi391 – 405Combined sources15
Helixi411 – 419Combined sources9
Helixi423 – 433Combined sources11
Beta strandi434 – 436Combined sources3
Beta strandi439 – 445Combined sources7
Helixi447 – 449Combined sources3
Helixi453 – 461Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HR6X-ray2.50B/D/F/H21-462[»]
1HR7X-ray2.55B/D/F/H21-462[»]
1HR8X-ray2.70B/D/F/H21-462[»]
1HR9X-ray3.01B/D/F/H21-462[»]
ProteinModelPortaliP10507
SMRiP10507
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10507

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M16 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00550000074701
HOGENOMiHOG000242450
InParanoidiP10507
KOiK17732
OMAiEDTGSRM
OrthoDBiEOG092C25X9

Family and domain databases

InterProiView protein in InterPro
IPR011249 Metalloenz_LuxS/M16
IPR037718 MPP_beat
IPR011765 Pept_M16_N
IPR001431 Pept_M16_Zn_BS
IPR007863 Peptidase_M16_C
PANTHERiPTHR11851:SF103 PTHR11851:SF103, 1 hit
PfamiView protein in Pfam
PF00675 Peptidase_M16, 1 hit
PF05193 Peptidase_M16_C, 1 hit
SUPFAMiSSF63411 SSF63411, 2 hits
PROSITEiView protein in PROSITE
PS00143 INSULINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10507-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSRTASKFR NTRRLLSTIS SQIPGTRTSK LPNGLTIATE YIPNTSSATV
60 70 80 90 100
GIFVDAGSRA ENVKNNGTAH FLEHLAFKGT QNRSQQGIEL EIENIGSHLN
110 120 130 140 150
AYTSRENTVY YAKSLQEDIP KAVDILSDIL TKSVLDNSAI ERERDVIIRE
160 170 180 190 200
SEEVDKMYDE VVFDHLHEIT YKDQPLGRTI LGPIKNIKSI TRTDLKDYIT
210 220 230 240 250
KNYKGDRMVL AGAGAVDHEK LVQYAQKYFG HVPKSESPVP LGSPRGPLPV
260 270 280 290 300
FCRGERFIKE NTLPTTHIAI ALEGVSWSAP DYFVALATQA IVGNWDRAIG
310 320 330 340 350
TGTNSPSPLA VAASQNGSLA NSYMSFSTSY ADSGLWGMYI VTDSNEHNVQ
360 370 380 390 400
LIVNEILKEW KRIKSGKISD AEVNRAKAQL KAALLLSLDG STAIVEDIGR
410 420 430 440 450
QVVTTGKRLS PEEVFEQVDK ITKDDIIMWA NYRLQNKPVS MVALGNTSTV
460
PNVSYIEEKL NQ
Length:462
Mass (Da):51,084
Last modified:July 1, 1989 - v1
Checksum:i084CE63C495EDFC4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07649 Genomic DNA Translation: CAA30489.1
U51921 Genomic DNA Translation: AAB67487.1
AY693198 Genomic DNA Translation: AAT93217.1
BK006945 Genomic DNA Translation: DAA09485.1
PIRiS00552
RefSeqiNP_013264.1, NM_001182050.1

Genome annotation databases

EnsemblFungiiYLR163C; YLR163C; YLR163C
GeneIDi850860
KEGGisce:YLR163C

Similar proteinsi

Entry informationi

Entry nameiMPPB_YEAST
AccessioniPrimary (citable) accession number: P10507
Secondary accession number(s): D6VYG9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 23, 2018
This is version 186 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

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