P10503 (PUTA_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional protein putA

Including the following 2 domains:

1. Proline dehydrogenase
   EC=1.5.99.8
   Alternative name(s):
   Proline oxidase
2. Delta-1-pyrroline-5-carboxylate dehydrogenase
   Short name=P5C dehydrogenase
   EC=1.5.1.12

Gene names

Name:	putA
Ordered Locus Names:	STM1124

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	1320 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Oxidizes proline to glutamate for use as a carbon and nitrogen source and also function as a transcriptional repressor of the put operon.
Catalytic activity	L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor. (S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H.
Cofactor	FAD.
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2. Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.
Induction	By proline, autorepression and catabolite repression, and is potentially nitrogen controlled.
Sequence similarities	In the N-terminal section; belongs to the proline dehydrogenase family. In the C-terminal section; belongs to the aldehyde dehydrogenase family.
Sequence caution	The sequence AAL20055.1 differs from that shown. Reason: Frameshift at position 1048.

Ontologies

Keywords
Biological process	Proline metabolism Transcription Transcription regulation
Ligand	DNA-binding FAD Flavoprotein NAD
Molecular function	Oxidoreductase Repressor
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: InterPro proline biosynthetic process Inferred from electronic annotation. Source: InterPro proline catabolic process Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro proline dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1320	1320	Bifunctional protein putA		PRO_0000056528
Regions
	Region	228 – 574	347	Proline dehydrogenase
	Region	653 – 1119	467	Aldehyde dehydrogenase
Sites
	Active site	883	1	By similarity
	Active site	917	1	By similarity
Experimental info
	Sequence conflict	850	1	G → R in CAA50193. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P10503 [UniParc]. Last modified January 23, 2002. Version 4. Checksum: 6F38C93EE6A5FD4D	FASTA	1,320	144,090
	10 20 30 40 50 60 MGTTTMGVKL DDATRERIKM AASRIDRTPH WLIKQAIFSY LDKLENSDTL PELPALFVGA 70 80 90 100 110 120 ANESEEPVAP QDEPHQPFLE FAEQILPQSV SRAAITAAWR RPETDAVSML MEQARLSPPV 130 140 150 160 170 180 AEQAHKLAYQ LAEKLRNQKS ASGRAGMVQG LLQEFSLSSQ EGVALMCLAE ALLRIPDKAT 190 200 210 220 230 240 RDALIRDKIS NGNWQSHIGR SPSLFVNAAT WGLLFTGRLV STHNEANLSR SLNRIIGKSG 250 260 270 280 290 300 EPLIRKGVDM AMRLMGEQFV TGETIAQALA NARKLEEKGF RYSYDMLGEA ALTAADAQAY 310 320 330 340 350 360 MVSYQQAIHA IGKASNGRGI YEGPGISIKL SALHPRYSRA QYDRVMEELY PRLKSLTLLA 370 380 390 400 410 420 RQYDIGLNID AEEADRLEIS LDLLEKLCFE PELAGWNGIG FVIQAYQKRC PLVIDYLVDL 430 440 450 460 470 480 ASRSRRRLMI RLVKGAYWDS EIKRAQMEGL EGYPVYTRKV YTDVSYLACA KKLLAVPNLI 490 500 510 520 530 540 YPQFATHNAH TLAAIYHLAG QNYYPGQYEF QCLHGMGEPL YEQVTGKVAD GKLNRPCRIY 550 560 570 580 590 600 APVGTHETLL AYLVRRLLEN GANTSFVNRI ADATLPLDEL VADPVEAVEK LAQQEGQAGI 610 620 630 640 650 660 PHPKIPLPRD LYGEGRINSA GLDLANEHRL ASLSSALLSN AMQKWQAKPV LEQPVADGEM 670 680 690 700 710 720 TPVINPAEPK DIVGWGREAT ESEVEQALQN AVNQAPVWFA TPPQERAAIL QRAAVLMEDQ 730 740 750 760 770 780 MQQLIGLLVR EAGKTFSNAI AEVREAVDFL HYYAGQVRDD FDNETHRPLG PVVCISPWNF 790 800 810 820 830 840 PLAIFTGQIA AALAAGNSVL AKPAEQTSLI AAQGIAILLE AGVPPGVVQL LPGRGETVGA 850 860 870 880 890 900 QLTADARVRG VMFTGSTEVA TLLQRNIATR LDAQGRPIPL IAETGGMNAM IVDSSALTEQ 910 920 930 940 950 960 VVVDVLASAF DSAGQRCSAL RVLCLQDDIA EHTLKMLRGA MAECRMGNPG RLTTDIGPVI 970 980 990 1000 1010 1020 DSEAKANIER HIQTMRAKGR PVFQAARENS DDAQEWQTGT FVMPTLIELE NFAELEKEVF 1030 1040 1050 1060 1070 1080 GPVLHVVRYN RNQLAELIEQ INASGYGLTL GVHTRIDETI AQVTGSAHVG NLYVNRNMVG 1090 1100 1110 1120 1130 1140 AVVGVQPFGG EGLSGTGPKA GGPLYLYRLL AHRPPNALNT TLTRQDARYP VDAQLKTTLL 1150 1160 1170 1180 1190 1200 APLTALTQWA ADRPALQTLC RQFADLAQAG TQRLLPGPTG ERNTWTLLPR ERVLCLADDE 1210 1220 1230 1240 1250 1260 QDALTQLAAV LAVGSQALWS DDAFHRDLAK RLPAAVAARV QFAKAETLMA QPFDAVIFHG 1270 1280 1290 1300 1310 1320 DSDKLRTVCE AVAAREGAIV SVQGFARGES NILLERLYIE RSLSVNTAAA GGNASLMTIG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence of the putA gene from Salmonella typhimurium: a bifunctional membrane-associated dehydrogenase that binds DNA." Allen S.W., Senti-Willis A.E., Maloy S.R. Nucleic Acids Res. 21:1676-1676(1993) [PubMed: 8479928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Maloy S.R. Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[4]	"Regulation of proline utilization in Salmonella typhimurium: molecular characterization of the put operon, and DNA sequence of the put control region." Hahn D.R., Myers R.S., Kent C.R., Maloy S.R. Mol. Gen. Genet. 213:125-133(1988) [PubMed: 2851701] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-26.
[5]	"Regulation of proline utilization in Salmonella typhimurium: a membrane-associated dehydrogenase binds DNA in vitro." Ostrovsky de Spicer P., O'Brien K., Maloy S. J. Bacteriol. 173:211-219(1991) [PubMed: 1987118] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-26. Strain: LT2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X70843 Genomic DNA. Translation: CAA50193.1. AE006468 Genomic DNA. Translation: AAL20055.1. Frameshift. X12569 Genomic DNA. Translation: CAA31081.1. Sequence problems.
PIR	S66279.
3D structure databases
ProteinModelPortal	P10503.
SMR	P10503. Positions 2-49, 87-612.
ModBase	Search...
PTM databases
PhosSite	P10503.
Proteomic databases
PRIDE	P10503.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GenomeReviews	Gene locus STM1124 in contig AE006468_GR.
Phylogenomic databases
HOGENOM	HBG288861.
OMA	QPFLEFA.
Enzyme and pathway databases
BioCyc	STYP99287:STM1124-MONOMER.
Family and domain databases
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR005933. Delta1-pyrroline-5-COlate_DH-3. IPR024090. PRODH_PutA_dom_I. IPR024089. PRODH_PutA_dom_I/II. IPR024082. PRODH_PutA_dom_II. IPR002872. Proline_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit. G3DSA:1.10.2060.10. G3DSA:1.10.2060.10. 1 hit. G3DSA:1.20.5.550. G3DSA:1.20.5.550. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. PF01619. Pro_dh. 1 hit. [Graphical view]
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit. SSF81935. SSF81935. 1 hit.
TIGRFAMs	TIGR01238. D1pyr5carbox3. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUTA_SALTY

Accession

Primary (citable) accession number: P10503

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	January 23, 2002
Last modified:	October 19, 2011
This is version 104 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program