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P10499 (KCNA1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium voltage-gated channel subfamily A member 1
Alternative name(s):
RBKI
RCK1
Voltage-gated potassium channel subunit Kv1.1
Gene names
Name:Kcna1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length495 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

Subunit structure

Heterotetramer of potassium channel proteins. Binds KCNAB2 By similarity. Binds PDZ domains of DLG1, DLG2 and DLG4. Interacts with LGI1 within a complex containing LGI1, KCNA4 and KCNAB1. Ref.4 Ref.5 Ref.6

Subcellular location

Membrane; Multi-pass membrane protein.

Domain

The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Post-translational modification

Palmitoylated on Cys-243; which may be required for membrane targeting By similarity.

Sequence similarities

Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.1/KCNA1 sub-subfamily. [View classification]

RNA editing

Edited at position 400.
Partially edited. RNA editing is at an average of 50% in the whole brain. Ref.3

Binary interactions

With

Entry

#Exp.

IntAct

Notes

DLG4P783522EBI-631463,EBI-80389From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 495495Potassium voltage-gated channel subfamily A member 1
PRO_0000053970

Regions

Transmembrane168 – 18619Helical; Name=Segment S1; Potential
Transmembrane221 – 24222Helical; Name=Segment S2; Potential
Transmembrane254 – 27421Helical; Name=Segment S3; Potential
Transmembrane290 – 30920Helical; Voltage-sensor; Name=Segment S4; Potential
Transmembrane326 – 34520Helical; Name=Segment S5; Potential
Transmembrane387 – 40822Helical; Name=Segment S6; Potential
Motif372 – 3776Selectivity filter By similarity
Motif493 – 4953PDZ-binding

Amino acid modifications

Modified residue3221Phosphoserine; by PKA Potential
Modified residue4451Phosphoserine; by PKA Potential
Lipidation2431S-palmitoyl cysteine By similarity
Glycosylation2071N-linked (GlcNAc...) Potential

Natural variations

Natural variant4001I → V in RNA edited version.

Secondary structure

.................. 495
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P10499 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 29804463133F5D31

FASTA49556,379
        10         20         30         40         50         60 
MTVMSGENAD EASAAPGHPQ DGSYPRQADH DDHECCERVV INISGLRFET QLKTLAQFPN 

        70         80         90        100        110        120 
TLLGNPKKRM RYFDPLRNEY FFDRNRPSFD AILYYYQSGG RLRRPVNVPL DMFSEEIKFY 

       130        140        150        160        170        180 
ELGEEAMEKF REDEGFIKEE ERPLPEKEYQ RQVWLLFEYP ESSGPARVIA IVSVMVILIS 

       190        200        210        220        230        240 
IVIFCLETLP ELKDDKDFTG TIHRIDNTTV IYTSNIFTDP FFIVETLCII WFSFELVVRF 

       250        260        270        280        290        300 
FACPSKTDFF KNIMNFIDIV AIIPYFITLG TEIAEQEGNQ KGEQATSLAI LRVIRLVRVF 

       310        320        330        340        350        360 
RIFKLSRHSK GLQILGQTLK ASMRELGLLI FFLFIGVILF SSAVYFAEAE EAESHFSSIP 

       370        380        390        400        410        420 
DAFWWAVVSM TTVGYGDMYP VTIGGKIVGS LCAIAGVLTI ALPVPVIVSN FNYFYHRETE 

       430        440        450        460        470        480 
GEEQAQLLHV SSPNLASDSD LSRRSSSTIS KSEYMEIEED MNNSIAHYRQ ANIRTGNCTA 

       490 
TDQNCVNKSK LLTDV 

« Hide

References

[1]"Structure of the voltage-dependent potassium channel is highly conserved from Drosophila to vertebrate central nervous systems."
Baumann A., Grupe A., Ackermann A., Pongs O.
EMBO J. 7:2457-2463(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Expression of a cloned rat brain potassium channel in Xenopus oocytes."
Christie M.J., Adelman J.P., Douglass J., North R.A.
Science 244:221-224(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]"Nervous system targets of RNA editing identified by comparative genomics."
Hoopengardner B., Bhalla T., Staber C., Reenan R.
Science 301:832-836(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: RNA EDITING OF POSITION 400.
[4]"Clustering of Shaker-type K+ channels by interaction with a family of membrane-associated guanylate kinases."
Kim E., Niethammer M., Rothschild A., Jan Y.N., Sheng M.
Nature 378:85-88(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DLG1; DLG2 AND DLG4.
[5]"The epilepsy-linked Lgi1 protein assembles into presynaptic Kv1 channels and inhibits inactivation by Kvbeta1."
Schulte U., Thumfart J.-O., Kloecker N., Sailer C.A., Bildl W., Biniossek M., Dehn D., Deller T., Eble S., Abbass K., Wangler T., Knaus H.-G., Fakler B.
Neuron 49:697-706(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH LGI1; KCNA4 AND KCNAB1.
[6]"Structure of the cytoplasmic beta subunit-T1 assembly of voltage-dependent K+ channels."
Gulbis J.M., Zhou M., Mann S., MacKinnon R.
Science 289:123-127(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 29-128, INTERACTION WITH KCNAB2, TETRAMERIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X12589 mRNA. Translation: CAA31102.1.
M26161 mRNA. Translation: AAA41982.1.
PIRB39113.
RefSeqNP_775118.1. NM_173095.3.
UniGeneRn.9769.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EXBX-ray2.10E27-129[»]
ProteinModelPortalP10499.
SMRP10499. Positions 36-419.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid246675. 2 interactions.
IntActP10499. 2 interactions.
MINTMINT-138588.
STRING10116.ENSRNOP00000026731.

Chemistry

ChEMBLCHEMBL5190.
GuidetoPHARMACOLOGY538.

PTM databases

PhosphoSiteP10499.

Proteomic databases

PaxDbP10499.
PRIDEP10499.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000026731; ENSRNOP00000026731; ENSRNOG00000019750.
GeneID24520.
KEGGrno:24520.
UCSCRGD:2949. rat.

Organism-specific databases

CTD3736.
RGD2949. Kcna1.

Phylogenomic databases

eggNOGCOG1226.
GeneTreeENSGT00740000115256.
HOGENOMHOG000231015.
HOVERGENHBG052230.
InParanoidP10499.
KOK04874.
OMAEHDDHEC.
OrthoDBEOG7M0NRD.
PhylomeDBP10499.
TreeFamTF313103.

Gene expression databases

GenevestigatorP10499.

Family and domain databases

Gene3D1.20.120.350. 1 hit.
3.30.710.10. 1 hit.
InterProIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR004048. K_chnl_volt-dep_Kv1.1.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERPTHR11537. PTHR11537. 1 hit.
PfamPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSPR00169. KCHANNEL.
PR01508. KV11CHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.
SMARTSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMSSF54695. SSF54695. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP10499.
NextBio603561.
PROP10499.

Entry information

Entry nameKCNA1_RAT
AccessionPrimary (citable) accession number: P10499
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 11, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references