Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P10499

- KCNA1_RAT

UniProt

P10499 - KCNA1_RAT

Protein

Potassium voltage-gated channel subfamily A member 1

Gene

Kcna1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

    GO - Molecular functioni

    1. delayed rectifier potassium channel activity Source: RGD
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. protein homooligomerization Source: InterPro

    Keywords - Molecular functioni

    Ion channel, Potassium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    ReactomeiREACT_199159. Voltage gated Potassium channels.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium voltage-gated channel subfamily A member 1
    Alternative name(s):
    RBKI
    RCK1
    Voltage-gated potassium channel subunit Kv1.1
    Gene namesi
    Name:Kcna1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 4

    Organism-specific databases

    RGDi2949. Kcna1.

    Subcellular locationi

    GO - Cellular componenti

    1. apical plasma membrane Source: RGD
    2. dendrite Source: BHF-UCL
    3. juxtaparanode region of axon Source: Ensembl
    4. neuronal cell body Source: BHF-UCL
    5. voltage-gated potassium channel complex Source: InterPro

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 495495Potassium voltage-gated channel subfamily A member 1PRO_0000053970Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi207 – 2071N-linked (GlcNAc...)Sequence Analysis
    Lipidationi243 – 2431S-palmitoyl cysteineBy similarity
    Modified residuei322 – 3221Phosphoserine; by PKASequence Analysis
    Modified residuei445 – 4451Phosphoserine; by PKASequence Analysis

    Post-translational modificationi

    Palmitoylated on Cys-243; which may be required for membrane targeting.By similarity

    Keywords - PTMi

    Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PaxDbiP10499.
    PRIDEiP10499.

    PTM databases

    PhosphoSiteiP10499.

    Expressioni

    Gene expression databases

    GenevestigatoriP10499.

    Interactioni

    Subunit structurei

    Heterotetramer of potassium channel proteins. Binds KCNAB2 By similarity. Binds PDZ domains of DLG1, DLG2 and DLG4. Interacts with LGI1 within a complex containing LGI1, KCNA4 and KCNAB1.By similarity3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    DLG4P783522EBI-631463,EBI-80389From a different organism.

    Protein-protein interaction databases

    BioGridi246675. 2 interactions.
    IntActiP10499. 2 interactions.
    MINTiMINT-138588.
    STRINGi10116.ENSRNOP00000026731.

    Structurei

    Secondary structure

    1
    495
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi38 – 436
    Beta strandi46 – 516
    Helixi52 – 565
    Turni62 – 643
    Helixi66 – 694
    Helixi70 – 723
    Turni75 – 784
    Beta strandi79 – 824
    Helixi86 – 9712
    Helixi110 – 11910

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1EXBX-ray2.10E27-129[»]
    ProteinModelPortaliP10499.
    SMRiP10499. Positions 36-419.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP10499.

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei168 – 18619Helical; Name=Segment S1Sequence AnalysisAdd
    BLAST
    Transmembranei221 – 24222Helical; Name=Segment S2Sequence AnalysisAdd
    BLAST
    Transmembranei254 – 27421Helical; Name=Segment S3Sequence AnalysisAdd
    BLAST
    Transmembranei290 – 30920Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
    BLAST
    Transmembranei326 – 34520Helical; Name=Segment S5Sequence AnalysisAdd
    BLAST
    Transmembranei387 – 40822Helical; Name=Segment S6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi372 – 3776Selectivity filterBy similarity
    Motifi493 – 4953PDZ-binding

    Domaini

    The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1226.
    GeneTreeiENSGT00740000115256.
    HOGENOMiHOG000231015.
    HOVERGENiHBG052230.
    InParanoidiP10499.
    KOiK04874.
    OMAiEHDDHEC.
    OrthoDBiEOG7M0NRD.
    PhylomeDBiP10499.
    TreeFamiTF313103.

    Family and domain databases

    Gene3Di1.20.120.350. 1 hit.
    3.30.710.10. 1 hit.
    InterProiIPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR003091. K_chnl.
    IPR003968. K_chnl_volt-dep_Kv.
    IPR003972. K_chnl_volt-dep_Kv1.
    IPR004048. K_chnl_volt-dep_Kv1.1.
    IPR003131. T1-type_BTB.
    IPR028325. VG_K_chnl.
    [Graphical view]
    PANTHERiPTHR11537. PTHR11537. 1 hit.
    PfamiPF02214. BTB_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view]
    PRINTSiPR00169. KCHANNEL.
    PR01508. KV11CHANNEL.
    PR01491. KVCHANNEL.
    PR01496. SHAKERCHANEL.
    SMARTiSM00225. BTB. 1 hit.
    [Graphical view]
    SUPFAMiSSF54695. SSF54695. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P10499-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTVMSGENAD EASAAPGHPQ DGSYPRQADH DDHECCERVV INISGLRFET    50
    QLKTLAQFPN TLLGNPKKRM RYFDPLRNEY FFDRNRPSFD AILYYYQSGG 100
    RLRRPVNVPL DMFSEEIKFY ELGEEAMEKF REDEGFIKEE ERPLPEKEYQ 150
    RQVWLLFEYP ESSGPARVIA IVSVMVILIS IVIFCLETLP ELKDDKDFTG 200
    TIHRIDNTTV IYTSNIFTDP FFIVETLCII WFSFELVVRF FACPSKTDFF 250
    KNIMNFIDIV AIIPYFITLG TEIAEQEGNQ KGEQATSLAI LRVIRLVRVF 300
    RIFKLSRHSK GLQILGQTLK ASMRELGLLI FFLFIGVILF SSAVYFAEAE 350
    EAESHFSSIP DAFWWAVVSM TTVGYGDMYP VTIGGKIVGS LCAIAGVLTI 400
    ALPVPVIVSN FNYFYHRETE GEEQAQLLHV SSPNLASDSD LSRRSSSTIS 450
    KSEYMEIEED MNNSIAHYRQ ANIRTGNCTA TDQNCVNKSK LLTDV 495
    Length:495
    Mass (Da):56,379
    Last modified:April 1, 1990 - v1
    Checksum:i29804463133F5D31
    GO

    RNA editingi

    Partially edited. RNA editing is at an average of 50% in the whole brain.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti400 – 4001I → V in RNA edited version.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12589 mRNA. Translation: CAA31102.1.
    M26161 mRNA. Translation: AAA41982.1.
    PIRiB39113.
    RefSeqiNP_775118.1. NM_173095.3.
    UniGeneiRn.9769.

    Genome annotation databases

    EnsembliENSRNOT00000026731; ENSRNOP00000026731; ENSRNOG00000019750.
    GeneIDi24520.
    KEGGirno:24520.
    UCSCiRGD:2949. rat.

    Keywords - Coding sequence diversityi

    RNA editing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12589 mRNA. Translation: CAA31102.1 .
    M26161 mRNA. Translation: AAA41982.1 .
    PIRi B39113.
    RefSeqi NP_775118.1. NM_173095.3.
    UniGenei Rn.9769.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1EXB X-ray 2.10 E 27-129 [» ]
    ProteinModelPortali P10499.
    SMRi P10499. Positions 36-419.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246675. 2 interactions.
    IntActi P10499. 2 interactions.
    MINTi MINT-138588.
    STRINGi 10116.ENSRNOP00000026731.

    Chemistry

    ChEMBLi CHEMBL5190.
    GuidetoPHARMACOLOGYi 538.

    PTM databases

    PhosphoSitei P10499.

    Proteomic databases

    PaxDbi P10499.
    PRIDEi P10499.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000026731 ; ENSRNOP00000026731 ; ENSRNOG00000019750 .
    GeneIDi 24520.
    KEGGi rno:24520.
    UCSCi RGD:2949. rat.

    Organism-specific databases

    CTDi 3736.
    RGDi 2949. Kcna1.

    Phylogenomic databases

    eggNOGi COG1226.
    GeneTreei ENSGT00740000115256.
    HOGENOMi HOG000231015.
    HOVERGENi HBG052230.
    InParanoidi P10499.
    KOi K04874.
    OMAi EHDDHEC.
    OrthoDBi EOG7M0NRD.
    PhylomeDBi P10499.
    TreeFami TF313103.

    Enzyme and pathway databases

    Reactomei REACT_199159. Voltage gated Potassium channels.

    Miscellaneous databases

    EvolutionaryTracei P10499.
    NextBioi 603561.
    PROi P10499.

    Gene expression databases

    Genevestigatori P10499.

    Family and domain databases

    Gene3Di 1.20.120.350. 1 hit.
    3.30.710.10. 1 hit.
    InterProi IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR003091. K_chnl.
    IPR003968. K_chnl_volt-dep_Kv.
    IPR003972. K_chnl_volt-dep_Kv1.
    IPR004048. K_chnl_volt-dep_Kv1.1.
    IPR003131. T1-type_BTB.
    IPR028325. VG_K_chnl.
    [Graphical view ]
    PANTHERi PTHR11537. PTHR11537. 1 hit.
    Pfami PF02214. BTB_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view ]
    PRINTSi PR00169. KCHANNEL.
    PR01508. KV11CHANNEL.
    PR01491. KVCHANNEL.
    PR01496. SHAKERCHANEL.
    SMARTi SM00225. BTB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54695. SSF54695. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structure of the voltage-dependent potassium channel is highly conserved from Drosophila to vertebrate central nervous systems."
      Baumann A., Grupe A., Ackermann A., Pongs O.
      EMBO J. 7:2457-2463(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Expression of a cloned rat brain potassium channel in Xenopus oocytes."
      Christie M.J., Adelman J.P., Douglass J., North R.A.
      Science 244:221-224(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    3. "Nervous system targets of RNA editing identified by comparative genomics."
      Hoopengardner B., Bhalla T., Staber C., Reenan R.
      Science 301:832-836(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: RNA EDITING OF POSITION 400.
    4. "Clustering of Shaker-type K+ channels by interaction with a family of membrane-associated guanylate kinases."
      Kim E., Niethammer M., Rothschild A., Jan Y.N., Sheng M.
      Nature 378:85-88(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DLG1; DLG2 AND DLG4.
    5. "The epilepsy-linked Lgi1 protein assembles into presynaptic Kv1 channels and inhibits inactivation by Kvbeta1."
      Schulte U., Thumfart J.-O., Kloecker N., Sailer C.A., Bildl W., Biniossek M., Dehn D., Deller T., Eble S., Abbass K., Wangler T., Knaus H.-G., Fakler B.
      Neuron 49:697-706(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH LGI1; KCNA4 AND KCNAB1.
    6. "Structure of the cytoplasmic beta subunit-T1 assembly of voltage-dependent K+ channels."
      Gulbis J.M., Zhou M., Mann S., MacKinnon R.
      Science 289:123-127(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 29-128, INTERACTION WITH KCNAB2, TETRAMERIZATION.

    Entry informationi

    Entry nameiKCNA1_RAT
    AccessioniPrimary (citable) accession number: P10499
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 137 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3