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P10499

- KCNA1_RAT

UniProt

P10499 - KCNA1_RAT

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Protein

Potassium voltage-gated channel subfamily A member 1

Gene

Kcna1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

GO - Molecular functioni

  1. delayed rectifier potassium channel activity Source: RGD

GO - Biological processi

  1. protein homooligomerization Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiREACT_199159. Voltage gated Potassium channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily A member 1
Alternative name(s):
RBKI
RCK1
Voltage-gated potassium channel subunit Kv1.1
Gene namesi
Name:Kcna1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi2949. Kcna1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei168 – 18619Helical; Name=Segment S1Sequence AnalysisAdd
BLAST
Transmembranei221 – 24222Helical; Name=Segment S2Sequence AnalysisAdd
BLAST
Transmembranei254 – 27421Helical; Name=Segment S3Sequence AnalysisAdd
BLAST
Transmembranei290 – 30920Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
BLAST
Transmembranei326 – 34520Helical; Name=Segment S5Sequence AnalysisAdd
BLAST
Transmembranei387 – 40822Helical; Name=Segment S6Sequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: RGD
  2. dendrite Source: BHF-UCL
  3. juxtaparanode region of axon Source: Ensembl
  4. neuronal cell body Source: BHF-UCL
  5. voltage-gated potassium channel complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 495495Potassium voltage-gated channel subfamily A member 1PRO_0000053970Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi207 – 2071N-linked (GlcNAc...)Sequence Analysis
Lipidationi243 – 2431S-palmitoyl cysteineBy similarity
Modified residuei322 – 3221Phosphoserine; by PKASequence Analysis
Modified residuei445 – 4451Phosphoserine; by PKASequence Analysis

Post-translational modificationi

Palmitoylated on Cys-243; which may be required for membrane targeting.By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP10499.
PRIDEiP10499.

PTM databases

PhosphoSiteiP10499.

Expressioni

Gene expression databases

GenevestigatoriP10499.

Interactioni

Subunit structurei

Heterotetramer of potassium channel proteins. Binds KCNAB2 (By similarity). Binds PDZ domains of DLG1, DLG2 and DLG4. Interacts with LGI1 within a complex containing LGI1, KCNA4 and KCNAB1.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DLG4P783522EBI-631463,EBI-80389From a different organism.

Protein-protein interaction databases

BioGridi246675. 2 interactions.
IntActiP10499. 2 interactions.
MINTiMINT-138588.
STRINGi10116.ENSRNOP00000026731.

Structurei

Secondary structure

1
495
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi38 – 436Combined sources
Beta strandi46 – 516Combined sources
Helixi52 – 565Combined sources
Turni62 – 643Combined sources
Helixi66 – 694Combined sources
Helixi70 – 723Combined sources
Turni75 – 784Combined sources
Beta strandi79 – 824Combined sources
Helixi86 – 9712Combined sources
Helixi110 – 11910Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EXBX-ray2.10E27-129[»]
ProteinModelPortaliP10499.
SMRiP10499. Positions 36-419.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10499.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi372 – 3776Selectivity filterBy similarity
Motifi493 – 4953PDZ-binding

Domaini

The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1226.
GeneTreeiENSGT00760000118846.
HOGENOMiHOG000231015.
HOVERGENiHBG052230.
InParanoidiP10499.
KOiK04874.
OMAiEHDDHEC.
OrthoDBiEOG7M0NRD.
PhylomeDBiP10499.
TreeFamiTF313103.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
3.30.710.10. 1 hit.
InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR004048. K_chnl_volt-dep_Kv1.1.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 1 hit.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01508. KV11CHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.

Sequencei

Sequence statusi: Complete.

P10499-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTVMSGENAD EASAAPGHPQ DGSYPRQADH DDHECCERVV INISGLRFET
60 70 80 90 100
QLKTLAQFPN TLLGNPKKRM RYFDPLRNEY FFDRNRPSFD AILYYYQSGG
110 120 130 140 150
RLRRPVNVPL DMFSEEIKFY ELGEEAMEKF REDEGFIKEE ERPLPEKEYQ
160 170 180 190 200
RQVWLLFEYP ESSGPARVIA IVSVMVILIS IVIFCLETLP ELKDDKDFTG
210 220 230 240 250
TIHRIDNTTV IYTSNIFTDP FFIVETLCII WFSFELVVRF FACPSKTDFF
260 270 280 290 300
KNIMNFIDIV AIIPYFITLG TEIAEQEGNQ KGEQATSLAI LRVIRLVRVF
310 320 330 340 350
RIFKLSRHSK GLQILGQTLK ASMRELGLLI FFLFIGVILF SSAVYFAEAE
360 370 380 390 400
EAESHFSSIP DAFWWAVVSM TTVGYGDMYP VTIGGKIVGS LCAIAGVLTI
410 420 430 440 450
ALPVPVIVSN FNYFYHRETE GEEQAQLLHV SSPNLASDSD LSRRSSSTIS
460 470 480 490
KSEYMEIEED MNNSIAHYRQ ANIRTGNCTA TDQNCVNKSK LLTDV
Length:495
Mass (Da):56,379
Last modified:April 1, 1990 - v1
Checksum:i29804463133F5D31
GO

RNA editingi

Partially edited. RNA editing is at an average of 50% in the whole brain.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti400 – 4001I → V in RNA edited version.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12589 mRNA. Translation: CAA31102.1.
M26161 mRNA. Translation: AAA41982.1.
PIRiB39113.
RefSeqiNP_775118.1. NM_173095.3.
UniGeneiRn.9769.

Genome annotation databases

EnsembliENSRNOT00000026731; ENSRNOP00000026731; ENSRNOG00000019750.
GeneIDi24520.
KEGGirno:24520.
UCSCiRGD:2949. rat.

Keywords - Coding sequence diversityi

RNA editing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12589 mRNA. Translation: CAA31102.1 .
M26161 mRNA. Translation: AAA41982.1 .
PIRi B39113.
RefSeqi NP_775118.1. NM_173095.3.
UniGenei Rn.9769.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1EXB X-ray 2.10 E 27-129 [» ]
ProteinModelPortali P10499.
SMRi P10499. Positions 36-419.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 246675. 2 interactions.
IntActi P10499. 2 interactions.
MINTi MINT-138588.
STRINGi 10116.ENSRNOP00000026731.

Chemistry

ChEMBLi CHEMBL5190.
GuidetoPHARMACOLOGYi 538.

PTM databases

PhosphoSitei P10499.

Proteomic databases

PaxDbi P10499.
PRIDEi P10499.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000026731 ; ENSRNOP00000026731 ; ENSRNOG00000019750 .
GeneIDi 24520.
KEGGi rno:24520.
UCSCi RGD:2949. rat.

Organism-specific databases

CTDi 3736.
RGDi 2949. Kcna1.

Phylogenomic databases

eggNOGi COG1226.
GeneTreei ENSGT00760000118846.
HOGENOMi HOG000231015.
HOVERGENi HBG052230.
InParanoidi P10499.
KOi K04874.
OMAi EHDDHEC.
OrthoDBi EOG7M0NRD.
PhylomeDBi P10499.
TreeFami TF313103.

Enzyme and pathway databases

Reactomei REACT_199159. Voltage gated Potassium channels.

Miscellaneous databases

EvolutionaryTracei P10499.
NextBioi 603561.
PROi P10499.

Gene expression databases

Genevestigatori P10499.

Family and domain databases

Gene3Di 1.20.120.350. 1 hit.
3.30.710.10. 1 hit.
InterProi IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR004048. K_chnl_volt-dep_Kv1.1.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view ]
PANTHERi PTHR11537. PTHR11537. 1 hit.
Pfami PF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view ]
PRINTSi PR00169. KCHANNEL.
PR01508. KV11CHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.
SMARTi SM00225. BTB. 1 hit.
[Graphical view ]
SUPFAMi SSF54695. SSF54695. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Structure of the voltage-dependent potassium channel is highly conserved from Drosophila to vertebrate central nervous systems."
    Baumann A., Grupe A., Ackermann A., Pongs O.
    EMBO J. 7:2457-2463(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Expression of a cloned rat brain potassium channel in Xenopus oocytes."
    Christie M.J., Adelman J.P., Douglass J., North R.A.
    Science 244:221-224(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. "Nervous system targets of RNA editing identified by comparative genomics."
    Hoopengardner B., Bhalla T., Staber C., Reenan R.
    Science 301:832-836(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: RNA EDITING OF POSITION 400.
  4. "Clustering of Shaker-type K+ channels by interaction with a family of membrane-associated guanylate kinases."
    Kim E., Niethammer M., Rothschild A., Jan Y.N., Sheng M.
    Nature 378:85-88(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DLG1; DLG2 AND DLG4.
  5. "The epilepsy-linked Lgi1 protein assembles into presynaptic Kv1 channels and inhibits inactivation by Kvbeta1."
    Schulte U., Thumfart J.-O., Kloecker N., Sailer C.A., Bildl W., Biniossek M., Dehn D., Deller T., Eble S., Abbass K., Wangler T., Knaus H.-G., Fakler B.
    Neuron 49:697-706(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LGI1; KCNA4 AND KCNAB1.
  6. "Structure of the cytoplasmic beta subunit-T1 assembly of voltage-dependent K+ channels."
    Gulbis J.M., Zhou M., Mann S., MacKinnon R.
    Science 289:123-127(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 29-128, INTERACTION WITH KCNAB2, TETRAMERIZATION.

Entry informationi

Entry nameiKCNA1_RAT
AccessioniPrimary (citable) accession number: P10499
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 26, 2014
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3