P10499 (KCNA1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium voltage-gated channel subfamily A member 1

Alternative name(s):
RBKI
RCK1
Voltage-gated potassium channel subunit Kv1.1

Gene names

Name:

Kcna1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
Subunit structure	Heterotetramer of potassium channel proteins. Binds KCNAB2 By similarity. Binds PDZ domains of DLG1, DLG2 and DLG4. Interacts with LGI1 within a complex containing LGI1, KCNA4 and KCNAB1. Ref.4 Ref.5 Ref.6
Subcellular location	Membrane; Multi-pass membrane protein.
Domain	The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments. The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
Post-translational modification	Palmitoylated on Cys-243; which may be required for membrane targeting By similarity.
Sequence similarities	Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.1/KCNA1 sub-subfamily. [View classification]
RNA editing	Edited at position 400. Partially edited. RNA editing is at an average of 50% in the whole brain. Ref.3

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Coding sequence diversity	RNA editing
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Potassium channel Voltage-gated channel
PTM	Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein homooligomerization Inferred from electronic annotation. Source: InterPro
Cellular_component	apical plasma membrane Inferred from direct assay PubMed 14602579. Source: RGD dendrite Inferred from direct assay PubMed 19166515. Source: BHF-UCL juxtaparanode region of axon Inferred from electronic annotation. Source: Compara neuronal cell body Inferred from direct assay PubMed 19166515. Source: BHF-UCL voltage-gated potassium channel complex Inferred from electronic annotation. Source: InterPro
Molecular_function	delayed rectifier potassium channel activity Inferred from mutant phenotype PubMed 14602579. Source: RGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DLG4	P78352	2	EBI-631463,EBI-80389	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 495

495

Potassium voltage-gated channel subfamily A member 1

PRO_0000053970

Regions

Transmembrane

168 – 186

19

Helical; Name=Segment S1; Potential

Transmembrane

221 – 242

22

Helical; Name=Segment S2; Potential

Transmembrane

254 – 274

21

Helical; Name=Segment S3; Potential

Transmembrane

290 – 309

20

Helical; Voltage-sensor; Name=Segment S4; Potential

Transmembrane

326 – 345

20

Helical; Name=Segment S5; Potential

Transmembrane

387 – 408

22

Helical; Name=Segment S6; Potential

Motif

372 – 377

6

Selectivity filter By similarity

Motif

493 – 495

3

PDZ-binding

Amino acid modifications

Modified residue

322

1

Phosphoserine; by PKA Potential

Modified residue

445

1

Phosphoserine; by PKA Potential

Lipidation

243

1

S-palmitoyl cysteine By similarity

Glycosylation

207

1

N-linked (GlcNAc...) Potential

Natural variations

Natural variant

400

1

I → V in RNA edited version.

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

495

Helix Strand Turn

Details...

Beta strand

38 – 43

6

Beta strand

46 – 51

6

Helix

52 – 56

5

Turn

62 – 64

3

Helix

66 – 69

4

Helix

70 – 72

3

Turn

75 – 78

4

Beta strand

79 – 82

4

Helix

86 – 97

12

Helix

110 – 119

10

Sequences

	Sequence		Length	Mass (Da)
	P10499 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 29804463133F5D31	FASTA	495	56,379
	10 20 30 40 50 60 MTVMSGENAD EASAAPGHPQ DGSYPRQADH DDHECCERVV INISGLRFET QLKTLAQFPN 70 80 90 100 110 120 TLLGNPKKRM RYFDPLRNEY FFDRNRPSFD AILYYYQSGG RLRRPVNVPL DMFSEEIKFY 130 140 150 160 170 180 ELGEEAMEKF REDEGFIKEE ERPLPEKEYQ RQVWLLFEYP ESSGPARVIA IVSVMVILIS 190 200 210 220 230 240 IVIFCLETLP ELKDDKDFTG TIHRIDNTTV IYTSNIFTDP FFIVETLCII WFSFELVVRF 250 260 270 280 290 300 FACPSKTDFF KNIMNFIDIV AIIPYFITLG TEIAEQEGNQ KGEQATSLAI LRVIRLVRVF 310 320 330 340 350 360 RIFKLSRHSK GLQILGQTLK ASMRELGLLI FFLFIGVILF SSAVYFAEAE EAESHFSSIP 370 380 390 400 410 420 DAFWWAVVSM TTVGYGDMYP VTIGGKIVGS LCAIAGVLTI ALPVPVIVSN FNYFYHRETE 430 440 450 460 470 480 GEEQAQLLHV SSPNLASDSD LSRRSSSTIS KSEYMEIEED MNNSIAHYRQ ANIRTGNCTA 490 TDQNCVNKSK LLTDV

References

[1]	"Structure of the voltage-dependent potassium channel is highly conserved from Drosophila to vertebrate central nervous systems." Baumann A., Grupe A., Ackermann A., Pongs O. EMBO J. 7:2457-2463(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Expression of a cloned rat brain potassium channel in Xenopus oocytes." Christie M.J., Adelman J.P., Douglass J., North R.A. Science 244:221-224(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"Nervous system targets of RNA editing identified by comparative genomics." Hoopengardner B., Bhalla T., Staber C., Reenan R. Science 301:832-836(2003) [PubMed] [Europe PMC] [Abstract] Cited for: RNA EDITING OF POSITION 400.
[4]	"Clustering of Shaker-type K+ channels by interaction with a family of membrane-associated guanylate kinases." Kim E., Niethammer M., Rothschild A., Jan Y.N., Sheng M. Nature 378:85-88(1995) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH DLG1; DLG2 AND DLG4.
[5]	"The epilepsy-linked Lgi1 protein assembles into presynaptic Kv1 channels and inhibits inactivation by Kvbeta1." Schulte U., Thumfart J.-O., Kloecker N., Sailer C.A., Bildl W., Biniossek M., Dehn D., Deller T., Eble S., Abbass K., Wangler T., Knaus H.-G., Fakler B. Neuron 49:697-706(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH LGI1; KCNA4 AND KCNAB1.
[6]	"Structure of the cytoplasmic beta subunit-T1 assembly of voltage-dependent K+ channels." Gulbis J.M., Zhou M., Mann S., MacKinnon R. Science 289:123-127(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 29-128, INTERACTION WITH KCNAB2, TETRAMERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X12589 mRNA. Translation: CAA31102.1.
M26161 mRNA. Translation: AAA41982.1.

IPI

IPI00190644.

PIR

B39113.

RefSeq

NP_775118.1. NM_173095.3.

UniGene

Rn.9769.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EXB	X-ray	2.10	E	27-129	[»]

ProteinModelPortal

P10499.

SMR

P10499. Positions 36-419.

ModBase

Search...

Protein-protein interaction databases

IntAct

P10499. 2 interactions.

MINT

MINT-138588.

STRING

10116.ENSRNOP00000026731.

PTM databases

PhosphoSite

P10499.

Proteomic databases

PaxDb

P10499.

PRIDE

P10499.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000026731; ENSRNOP00000026731; ENSRNOG00000019750.

GeneID

24520.

KEGG

rno:24520.

UCSC

RGD:2949. rat.

Organism-specific databases

CTD

3736.

RGD

2949. Kcna1.

Phylogenomic databases

eggNOG

COG1226.

GeneTree

ENSGT00560000076957.

HOGENOM

HOG000231015.

HOVERGEN

HBG052230.

InParanoid

P10499.

KO

K04874.

OMA

YPRPAEH.

OrthoDB

EOG4DR9CB.

Gene expression databases

Genevestigator

P10499.

GermOnline

ENSRNOG00000019750. Rattus norvegicus.

Family and domain databases

Gene3D

1.20.120.350. 1 hit.
3.30.710.10. 1 hit.

InterPro

IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR005821. Ion_trans_dom.
IPR027359. K_channel_four-helix_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR004048. K_chnl_volt-dep_Kv1.1.
IPR003131. T1-type_BTB.
[Graphical view]

PANTHER

PTHR11537. PTHR11537. 1 hit.

Pfam

PF00520. Ion_trans. 1 hit.
PF02214. K_tetra. 1 hit.
[Graphical view]

PRINTS

PR00169. KCHANNEL.
PR01508. KV11CHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.

SMART

SM00225. BTB. 1 hit.
[Graphical view]

SUPFAM

SSF54695. BTB/POZ_fold. 1 hit.

ProtoNet

Search...

Other

ChEMBL

CHEMBL5190.

EvolutionaryTrace

P10499.

NextBio

603561.

Entry information

Entry name

KCNA1_RAT

Accession

Primary (citable) accession number: P10499

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	May 29, 2013
This is version 126 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program