P10485 (T1S1_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-1 restriction enzyme EcoR124II specificity protein
Short name=S.EcoR124II

Alternative name(s):
Type I restriction enzyme EcoR124II specificity protein
Short name=S protein

Gene names

Name:	hsdS
Synonyms:	hss

Encoded on

Plasmid IncFIV R124/3

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity. The EcoR124/3i enzyme recognizes 5'-GAAN6RTCG-3'.
Subunit structure	The type I restriction/modification system is composed of three polypeptides R, M and S.
Domain	Contains two DNA recognition domains, each specifying recognition of one of the two defined components of the target sequence.
Sequence similarities	Belongs to the type-I restriction system S methylase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	DNA-binding
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 404	404	Type-1 restriction enzyme EcoR124II specificity protein		PRO_0000198036

Sequences

	Sequence		Length	Mass (Da)
	P10485 [UniParc]. Last modified June 13, 2006. Version 2. Checksum: 164B882F0F594D16	FASTA	404	46,179
	10 20 30 40 50 60 MSEMSYLEKL LDGVEVEWLP LGEITKYEQP TKYLVKAKDY HDTYTIPVLT AGKTFILGYT 70 80 90 100 110 120 NETHGIYQAS KAPVIIFDDF TTANKWVDFD FKAKSSAMKM VTSCDDNKTL LKYVYYWLNT 130 140 150 160 170 180 LPSEFAEGDH KRQWISNYSQ KKIPIPCPDN PEKSLAIQSE IVRILDKFTA LTAELTAELN 190 200 210 220 230 240 MRKKQYNYYR DQLLSFKEGE VEWKTLGEIG KWYGGGTPSK NKIEFWENGS IPWISPKDMG 250 260 270 280 290 300 RTLVDSSEDY ITEEAVLHSS TKLIPANSIA IVVRSSILDK VLPSALIKVP ATLNQDMKAV 310 320 330 340 350 360 IPHENILVKY IYHMIGSRGS DILRAAKKTG GSVASIDSKK LFSFKIPVPN INEQQRIVEI 370 380 390 400 LDKFDTLTNS ITEGLPREIE LRQKQYEYYR DLLFSFPKPE TVSN

References

[1]	"Basis for changes in DNA recognition by the EcoR124 and EcoR124/3 type I DNA restriction and modification enzymes." Price C., Lingner J., Bickle J., Firman T.A., Glover S.W. J. Mol. Biol. 205:115-125(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Blundell A. Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 179-180; 340-344 AND C-TERMINUS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X13145 Genomic DNA. Translation: CAB37630.2.
PIR	S02167.
3D structure databases
ProteinModelPortal	P10485.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-17003N.
Protein family/group databases
REBASE	3647. S.EcoR124I.
Proteomic databases
PRIDE	P10485.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000055. Restrct_endonuc_typeI_HsdS. [Graphical view]
Pfam	PF01420. Methylase_S. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T1S1_ECOLX

Accession

Primary (citable) accession number: P10485

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	June 13, 2006
Last modified:	April 3, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program