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P10485

- T1S1_ECOLX

UniProt

P10485 - T1S1_ECOLX

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Protein
Type-1 restriction enzyme EcoR124II specificity protein
Gene
hsdS, hss
Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity. The EcoR124/3i enzyme recognizes 5'-GAAN6RTCG-3'.

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Restriction system

Keywords - Ligandi

DNA-binding

Protein family/group databases

REBASEi3647. S.EcoR124I.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-1 restriction enzyme EcoR124II specificity protein
Short name:
S.EcoR124II
Alternative name(s):
Type I restriction enzyme EcoR124II specificity protein
Short name:
S protein
Gene namesi
Name:hsdS
Synonyms:hss
Encoded oniPlasmid IncFIV R124/30 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 404404Type-1 restriction enzyme EcoR124II specificity protein
PRO_0000198036Add
BLAST

Proteomic databases

PRIDEiP10485.

Interactioni

Subunit structurei

The type I restriction/modification system is composed of three polypeptides R, M and S.

Protein-protein interaction databases

DIPiDIP-17003N.

Structurei

3D structure databases

ProteinModelPortaliP10485.

Family & Domainsi

Domaini

Contains two DNA recognition domains, each specifying recognition of one of the two defined components of the target sequence.

Sequence similaritiesi

Family and domain databases

InterProiIPR000055. Restrct_endonuc_typeI_HsdS.
[Graphical view]
PfamiPF01420. Methylase_S. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10485-1 [UniParc]FASTAAdd to Basket

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MSEMSYLEKL LDGVEVEWLP LGEITKYEQP TKYLVKAKDY HDTYTIPVLT    50
AGKTFILGYT NETHGIYQAS KAPVIIFDDF TTANKWVDFD FKAKSSAMKM 100
VTSCDDNKTL LKYVYYWLNT LPSEFAEGDH KRQWISNYSQ KKIPIPCPDN 150
PEKSLAIQSE IVRILDKFTA LTAELTAELN MRKKQYNYYR DQLLSFKEGE 200
VEWKTLGEIG KWYGGGTPSK NKIEFWENGS IPWISPKDMG RTLVDSSEDY 250
ITEEAVLHSS TKLIPANSIA IVVRSSILDK VLPSALIKVP ATLNQDMKAV 300
IPHENILVKY IYHMIGSRGS DILRAAKKTG GSVASIDSKK LFSFKIPVPN 350
INEQQRIVEI LDKFDTLTNS ITEGLPREIE LRQKQYEYYR DLLFSFPKPE 400
TVSN 404
Length:404
Mass (Da):46,179
Last modified:June 13, 2006 - v2
Checksum:i164B882F0F594D16
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X13145 Genomic DNA. Translation: CAB37630.2.
PIRiS02167.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X13145 Genomic DNA. Translation: CAB37630.2 .
PIRi S02167.

3D structure databases

ProteinModelPortali P10485.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-17003N.

Protein family/group databases

REBASEi 3647. S.EcoR124I.

Proteomic databases

PRIDEi P10485.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR000055. Restrct_endonuc_typeI_HsdS.
[Graphical view ]
Pfami PF01420. Methylase_S. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Basis for changes in DNA recognition by the EcoR124 and EcoR124/3 type I DNA restriction and modification enzymes."
    Price C., Lingner J., Bickle J., Firman T.A., Glover S.W.
    J. Mol. Biol. 205:115-125(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Blundell A.
    Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 179-180; 340-344 AND C-TERMINUS.

Entry informationi

Entry nameiT1S1_ECOLX
AccessioniPrimary (citable) accession number: P10485
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: June 13, 2006
Last modified: April 3, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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