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P10485 (T1S1_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-1 restriction enzyme EcoR124II specificity protein

Short name=S.EcoR124II
Alternative name(s):
Type I restriction enzyme EcoR124II specificity protein
Short name=S protein
Gene names
Name:hsdS
Synonyms:hss
Encoded onPlasmid IncFIV R124/3
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length404 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity. The EcoR124/3i enzyme recognizes 5'-GAAN6RTCG-3'.

Subunit structure

The type I restriction/modification system is composed of three polypeptides R, M and S.

Domain

Contains two DNA recognition domains, each specifying recognition of one of the two defined components of the target sequence.

Sequence similarities

Belongs to the type-I restriction system S methylase family.

Ontologies

Keywords
   Biological processRestriction system
   LigandDNA-binding
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 404404Type-1 restriction enzyme EcoR124II specificity protein
PRO_0000198036

Sequences

Sequence LengthMass (Da)Tools
P10485 [UniParc].

Last modified June 13, 2006. Version 2.
Checksum: 164B882F0F594D16

FASTA40446,179
        10         20         30         40         50         60 
MSEMSYLEKL LDGVEVEWLP LGEITKYEQP TKYLVKAKDY HDTYTIPVLT AGKTFILGYT 

        70         80         90        100        110        120 
NETHGIYQAS KAPVIIFDDF TTANKWVDFD FKAKSSAMKM VTSCDDNKTL LKYVYYWLNT 

       130        140        150        160        170        180 
LPSEFAEGDH KRQWISNYSQ KKIPIPCPDN PEKSLAIQSE IVRILDKFTA LTAELTAELN 

       190        200        210        220        230        240 
MRKKQYNYYR DQLLSFKEGE VEWKTLGEIG KWYGGGTPSK NKIEFWENGS IPWISPKDMG 

       250        260        270        280        290        300 
RTLVDSSEDY ITEEAVLHSS TKLIPANSIA IVVRSSILDK VLPSALIKVP ATLNQDMKAV 

       310        320        330        340        350        360 
IPHENILVKY IYHMIGSRGS DILRAAKKTG GSVASIDSKK LFSFKIPVPN INEQQRIVEI 

       370        380        390        400 
LDKFDTLTNS ITEGLPREIE LRQKQYEYYR DLLFSFPKPE TVSN 

« Hide

References

[1]"Basis for changes in DNA recognition by the EcoR124 and EcoR124/3 type I DNA restriction and modification enzymes."
Price C., Lingner J., Bickle J., Firman T.A., Glover S.W.
J. Mol. Biol. 205:115-125(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Blundell A.
Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 179-180; 340-344 AND C-TERMINUS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X13145 Genomic DNA. Translation: CAB37630.2.
PIRS02167.

3D structure databases

ProteinModelPortalP10485.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-17003N.

Protein family/group databases

REBASE3647. S.EcoR124I.

Proteomic databases

PRIDEP10485.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000055. Restrct_endonuc_typeI_HsdS.
[Graphical view]
PfamPF01420. Methylase_S. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT1S1_ECOLX
AccessionPrimary (citable) accession number: P10485
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: June 13, 2006
Last modified: April 3, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries