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P10485

- T1S1_ECOLX

UniProt

P10485 - T1S1_ECOLX

Protein

Type-1 restriction enzyme EcoR124II specificity protein

Gene

hsdS

Organism
Escherichia coli
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 2 (13 Jun 2006)
      Previous versions | rss
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    Functioni

    The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity. The EcoR124/3i enzyme recognizes 5'-GAAN6RTCG-3'.

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    DNA-binding

    Protein family/group databases

    REBASEi3647. S.EcoR124I.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-1 restriction enzyme EcoR124II specificity protein
    Short name:
    S.EcoR124II
    Alternative name(s):
    Type I restriction enzyme EcoR124II specificity protein
    Short name:
    S protein
    Gene namesi
    Name:hsdS
    Synonyms:hss
    Encoded oniPlasmid IncFIV R124/30 Publication
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 404404Type-1 restriction enzyme EcoR124II specificity proteinPRO_0000198036Add
    BLAST

    Proteomic databases

    PRIDEiP10485.

    Interactioni

    Subunit structurei

    The type I restriction/modification system is composed of three polypeptides R, M and S.

    Protein-protein interaction databases

    DIPiDIP-17003N.

    Structurei

    3D structure databases

    ProteinModelPortaliP10485.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Contains two DNA recognition domains, each specifying recognition of one of the two defined components of the target sequence.

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR000055. Restrct_endonuc_typeI_HsdS.
    [Graphical view]
    PfamiPF01420. Methylase_S. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P10485-1 [UniParc]FASTAAdd to Basket

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    MSEMSYLEKL LDGVEVEWLP LGEITKYEQP TKYLVKAKDY HDTYTIPVLT    50
    AGKTFILGYT NETHGIYQAS KAPVIIFDDF TTANKWVDFD FKAKSSAMKM 100
    VTSCDDNKTL LKYVYYWLNT LPSEFAEGDH KRQWISNYSQ KKIPIPCPDN 150
    PEKSLAIQSE IVRILDKFTA LTAELTAELN MRKKQYNYYR DQLLSFKEGE 200
    VEWKTLGEIG KWYGGGTPSK NKIEFWENGS IPWISPKDMG RTLVDSSEDY 250
    ITEEAVLHSS TKLIPANSIA IVVRSSILDK VLPSALIKVP ATLNQDMKAV 300
    IPHENILVKY IYHMIGSRGS DILRAAKKTG GSVASIDSKK LFSFKIPVPN 350
    INEQQRIVEI LDKFDTLTNS ITEGLPREIE LRQKQYEYYR DLLFSFPKPE 400
    TVSN 404
    Length:404
    Mass (Da):46,179
    Last modified:June 13, 2006 - v2
    Checksum:i164B882F0F594D16
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13145 Genomic DNA. Translation: CAB37630.2.
    PIRiS02167.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13145 Genomic DNA. Translation: CAB37630.2 .
    PIRi S02167.

    3D structure databases

    ProteinModelPortali P10485.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-17003N.

    Protein family/group databases

    REBASEi 3647. S.EcoR124I.

    Proteomic databases

    PRIDEi P10485.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR000055. Restrct_endonuc_typeI_HsdS.
    [Graphical view ]
    Pfami PF01420. Methylase_S. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Basis for changes in DNA recognition by the EcoR124 and EcoR124/3 type I DNA restriction and modification enzymes."
      Price C., Lingner J., Bickle J., Firman T.A., Glover S.W.
      J. Mol. Biol. 205:115-125(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Blundell A.
      Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 179-180; 340-344 AND C-TERMINUS.

    Entry informationi

    Entry nameiT1S1_ECOLX
    AccessioniPrimary (citable) accession number: P10485
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: June 13, 2006
    Last modified: October 1, 2014
    This is version 73 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Plasmid

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3