Reviewed, UniProtKB/Swiss-Prot P10484 (T1M1_ECOLX)

Names and origin

Protein names

Recommended name:
    Type I restriction enzyme EcoR124II M protein
      Short name=M.EcoR124II
    EC=2.1.1.72

Gene names

Name:	hsdM
Synonyms:	hsm

Encoded on

Plasmid IncFIV R124/3

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Methylation of specific adenine residues; required for both restriction and modification activities By similarity. The EcoR124/3 I enzyme recognizes 5'-GAAN7RTCG-3'.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Subunit structure	The type I restriction/modification system is composed of three polypeptides R, M and S.
Miscellaneous	Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and Mg2+ as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 520	520	Type I restriction enzyme EcoR124II M protein		PRO_0000088023
Regions
	Region	198 – 203	6	S-adenosyl-L-methionine binding By similarity
	Region	230 – 232	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	254	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P10484-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 8D82E715F41D94C3	FASTA	520	58,013
	10 20 30 40 50 60 MKMTSIQQRA ELHRQIWQIA NDVRGSVDGW DFKQYVLGAL FYRFISENFS SYIEAGDDSI 70 80 90 100 110 120 CYAKLDDSVI TDDIKDDAIK TKGYFIYPSQ LFCNVAAKAN TNDRLNADLN SIFVAIESSA 130 140 150 160 170 180 YGYPSEADIK GLFADFDTTS NRLGNTVKDK NARLAAVLKG VEGLKLGDFN EHQIDLFGDA 190 200 210 220 230 240 YEFLISNYAA NAGKSGGEFF TPQHVSKLIA QLAMHGQTHV NKIYDPAAGS GSLLLQAKKQ 250 260 270 280 290 300 FDNHIIEEGF FGQEINHTTY NLARMNMFLH NINYDKFDIK LGNTLTEPHF RDEKPFDAIV 310 320 330 340 350 360 SNPPYSVKWI GSDDPTLIND ERFAPAGVLA PKSKADFAFV LHALNYLSAK GRAAIVCFPG 370 380 390 400 410 420 IFYRGGAEQK IRQYLVDNNY VETVISLAPN LFFGTTIAVN ILVLSKHKTD TNVQFIDASE 430 440 450 460 470 480 LFKKETNNNI LTDAHIEQIM QVFASKEDVA HLAKSVAFET VVANDYNLSV SSYVEAKDNR 490 500 510 520 EIIDIAELNA ELKTTVSKID QLRKDIDAIV AEIEGCEVQK

References

[1]	"Basis for changes in DNA recognition by the EcoR124 and EcoR124/3 type I DNA restriction and modification enzymes." Price C., Lingner J., Bickle J., Firman T.A., Glover S.W. J. Mol. Biol. 205:115-125(1989) [PubMed: 2784505] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X13145 Genomic DNA. Translation: CAA31541.1.
PIR	S02166.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:17004N.
Protein family/group databases
REBASE	3391. M.EcoR124II. 3392. M.EcoR124I.
Enzyme and pathway databases
BRENDA	2.1.1.72. 246.
Family and domain databases
InterPro	IPR003356. DNA_methylase_A-5. IPR002052. DNA_methylase_N6_adenine_CS. IPR002296. N12N6_MeTrfase. IPR004546. Restrict_endonuc_I_EcoRI_M. [Graphical view]
Pfam	PF02384. N6_Mtase. 1 hit. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
TIGRFAMs	TIGR00497. hsdM. 1 hit.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T1M1_ECOLX

Accession

Primary (citable) accession number: P10484

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	September 22, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)