Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P10481

- NANH_CLOPF

UniProt

P10481 - NANH_CLOPF

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Sialidase

Gene

nanH

Organism
Clostridium perfringens
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Sialidases have been suggested to be pathogenic factors in microbial infections.

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei37 – 371SubstrateBy similarity
Active sitei62 – 621Proton acceptorBy similarity
Binding sitei245 – 2451SubstrateSequence Analysis
Active sitei347 – 3471NucleophileBy similarity

GO - Molecular functioni

  1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
  2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
  3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC

GO - Biological processi

  1. pathogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

SABIO-RKP10481.

Protein family/group databases

CAZyiGH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase (EC:3.2.1.18)
Alternative name(s):
Neuraminidase
Gene namesi
Name:nanH
OrganismiClostridium perfringens
Taxonomic identifieri1502 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 382382SialidasePRO_0000208908Add
BLAST

Interactioni

Structurei

3D structure databases

ProteinModelPortaliP10481.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati71 – 8212BNR 1Add
BLAST
Repeati140 – 15112BNR 2Add
BLAST
Repeati208 – 21912BNR 3Add
BLAST
Repeati255 – 26612BNR 4Add
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 33 family.Curated
Contains 4 BNR repeats.Curated

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR002860. BNR_rpt.
IPR026856. Sialidase_fam.
IPR008377. Sialidase_trypan.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PTHR10628:SF5. PTHR10628:SF5. 1 hit.
PfamiPF02012. BNR. 3 hits.
[Graphical view]
PRINTSiPR01803. TCSIALIDASE.
SUPFAMiSSF50939. SSF50939. 1 hit.

Sequencei

Sequence statusi: Complete.

P10481-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCNKNNTFEK NLDISHKPEP LILFNKDNNI WNSKYFRIPN IQLLNDGTIL
60 70 80 90 100
TFSDIRYNGP DDHAYIDIAS ARSTDFGKTW SYNIAMKNNR IDSTYSRVMD
110 120 130 140 150
STTVITNTGR IILIAGSWNT NGNWAMTTST RRSDWSVQMI YSDDNGLTWS
160 170 180 190 200
NKIDLTKDSS KVKNQPSNTI GWLGGVGSGI VMDDGTIVMP AQISLRENNE
210 220 230 240 250
NNYYSLIIYS KDNGETWTMG NKVPNSNTSE NMVIELDGAL IMSTRYDYSG
260 270 280 290 300
YRAAYISHDL GTTWEIYEPL NGKILTGKGS GCQGSFIKAT TSNGHRIGLI
310 320 330 340 350
SAPKNTKGEY IRDNIAVYMI DFDDLSKGVQ EICIPYPEDG NKLGGGYSCL
360 370 380
SFKNNHLGIV YEANGNIEYQ DLTPYYSLIN KQ
Length:382
Mass (Da):42,813
Last modified:July 1, 1989 - v1
Checksum:iD50857BB50A4E886
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00963 Genomic DNA. Translation: CAA68780.1.
PIRiS01339.

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00963 Genomic DNA. Translation: CAA68780.1 .
PIRi S01339.

3D structure databases

ProteinModelPortali P10481.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P10481.
ChEMBLi CHEMBL5189.

Protein family/group databases

CAZyi GH33. Glycoside Hydrolase Family 33.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

SABIO-RK P10481.

Family and domain databases

Gene3Di 2.120.10.10. 1 hit.
InterProi IPR002860. BNR_rpt.
IPR026856. Sialidase_fam.
IPR008377. Sialidase_trypan.
IPR011040. Sialidases.
[Graphical view ]
PANTHERi PTHR10628. PTHR10628. 1 hit.
PTHR10628:SF5. PTHR10628:SF5. 1 hit.
Pfami PF02012. BNR. 3 hits.
[Graphical view ]
PRINTSi PR01803. TCSIALIDASE.
SUPFAMi SSF50939. SSF50939. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of a Clostridium perfringens sialidase gene."
    Roggentin P., Rothe B., Lottspeich F., Schauer R.
    FEBS Lett. 238:31-34(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 8-27.
    Strain: A99.

Entry informationi

Entry nameiNANH_CLOPF
AccessioniPrimary (citable) accession number: P10481
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 29, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3