Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P10481 (NANH_CLOPF) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sialidase

EC=3.2.1.18
Alternative name(s):
Neuraminidase
Gene names
Name:nanH
OrganismClostridium perfringens
Taxonomic identifier1502 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length382 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sialidases have been suggested to be pathogenic factors in microbial infections.

Catalytic activity

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Subcellular location

Secreted.

Sequence similarities

Belongs to the glycosyl hydrolase 33 family.

Contains 4 BNR repeats.

Ontologies

Keywords
   Cellular componentSecreted
   DomainRepeat
   Molecular functionGlycosidase
Hydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processpathogenesis

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionexo-alpha-(2->3)-sialidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

exo-alpha-(2->6)-sialidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

exo-alpha-(2->8)-sialidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 382382Sialidase
PRO_0000208908

Regions

Repeat71 – 8212BNR 1
Repeat140 – 15112BNR 2
Repeat208 – 21912BNR 3
Repeat255 – 26612BNR 4

Sites

Active site621Proton acceptor By similarity
Active site3471Nucleophile By similarity
Binding site371Substrate By similarity
Binding site2451Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
P10481 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: D50857BB50A4E886

FASTA38242,813
        10         20         30         40         50         60 
MCNKNNTFEK NLDISHKPEP LILFNKDNNI WNSKYFRIPN IQLLNDGTIL TFSDIRYNGP 

        70         80         90        100        110        120 
DDHAYIDIAS ARSTDFGKTW SYNIAMKNNR IDSTYSRVMD STTVITNTGR IILIAGSWNT 

       130        140        150        160        170        180 
NGNWAMTTST RRSDWSVQMI YSDDNGLTWS NKIDLTKDSS KVKNQPSNTI GWLGGVGSGI 

       190        200        210        220        230        240 
VMDDGTIVMP AQISLRENNE NNYYSLIIYS KDNGETWTMG NKVPNSNTSE NMVIELDGAL 

       250        260        270        280        290        300 
IMSTRYDYSG YRAAYISHDL GTTWEIYEPL NGKILTGKGS GCQGSFIKAT TSNGHRIGLI 

       310        320        330        340        350        360 
SAPKNTKGEY IRDNIAVYMI DFDDLSKGVQ EICIPYPEDG NKLGGGYSCL SFKNNHLGIV 

       370        380 
YEANGNIEYQ DLTPYYSLIN KQ 

« Hide

References

[1]"Cloning and sequencing of a Clostridium perfringens sialidase gene."
Roggentin P., Rothe B., Lottspeich F., Schauer R.
FEBS Lett. 238:31-34(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 8-27.
Strain: A99.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y00963 Genomic DNA. Translation: CAA68780.1.
PIRS01339.

3D structure databases

ProteinModelPortalP10481.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP10481.
ChEMBLCHEMBL5189.

Protein family/group databases

CAZyGH33. Glycoside Hydrolase Family 33.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP10481.

Family and domain databases

Gene3D2.120.10.10. 1 hit.
InterProIPR002860. BNR_rpt.
IPR026948. Sialidase/anhydrosialidase.
IPR026856. Sialidase_fam.
IPR008377. Sialidase_trypan.
IPR011040. Sialidases.
[Graphical view]
PANTHERPTHR10628. PTHR10628. 1 hit.
PTHR10628:SF5. PTHR10628:SF5. 1 hit.
PfamPF02012. BNR. 3 hits.
[Graphical view]
PRINTSPR01803. TCSIALIDASE.
SUPFAMSSF50939. SSF50939. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNANH_CLOPF
AccessionPrimary (citable) accession number: P10481
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: December 11, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries