P10481 (NANH_CLOPF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sialidase
EC=3.2.1.18

Alternative name(s):
Neuraminidase

Gene names

Name:

nanH

Organism

Clostridium perfringens

Taxonomic identifier

1502 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sialidases have been suggested to be pathogenic factors in microbial infections.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subcellular location	Secreted.
Sequence similarities	Belongs to the glycosyl hydrolase 33 family. Contains 4 BNR repeats.

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	exo-alpha-(2->3)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->6)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->8)-sialidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Sialidase		PRO_0000208908
Regions
	Repeat	71 – 82	12	BNR 1
	Repeat	140 – 151	12	BNR 2
	Repeat	208 – 219	12	BNR 3
	Repeat	255 – 266	12	BNR 4
Sites
	Active site	62	1	Proton acceptor By similarity
	Active site	347	1	Nucleophile By similarity
	Binding site	37	1	Substrate By similarity
	Binding site	245	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	P10481 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: D50857BB50A4E886	FASTA	382	42,813
	10 20 30 40 50 60 MCNKNNTFEK NLDISHKPEP LILFNKDNNI WNSKYFRIPN IQLLNDGTIL TFSDIRYNGP 70 80 90 100 110 120 DDHAYIDIAS ARSTDFGKTW SYNIAMKNNR IDSTYSRVMD STTVITNTGR IILIAGSWNT 130 140 150 160 170 180 NGNWAMTTST RRSDWSVQMI YSDDNGLTWS NKIDLTKDSS KVKNQPSNTI GWLGGVGSGI 190 200 210 220 230 240 VMDDGTIVMP AQISLRENNE NNYYSLIIYS KDNGETWTMG NKVPNSNTSE NMVIELDGAL 250 260 270 280 290 300 IMSTRYDYSG YRAAYISHDL GTTWEIYEPL NGKILTGKGS GCQGSFIKAT TSNGHRIGLI 310 320 330 340 350 360 SAPKNTKGEY IRDNIAVYMI DFDDLSKGVQ EICIPYPEDG NKLGGGYSCL SFKNNHLGIV 370 380 YEANGNIEYQ DLTPYYSLIN KQ

References

[1]	"Cloning and sequencing of a Clostridium perfringens sialidase gene." Roggentin P., Rothe B., Lottspeich F., Schauer R. FEBS Lett. 238:31-34(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 8-27. Strain: A99.

Web resources

Worthington enzyme manual

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y00963 Genomic DNA. Translation: CAA68780.1.
PIR	S01339.
3D structure databases
ProteinModelPortal	P10481.
ModBase	Search...
Protein family/group databases
CAZy	GH33. Glycoside Hydrolase Family 33.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.120.10.10. 1 hit.
InterPro	IPR002860. BNR_rpt. IPR026948. Sialidase. IPR026856. Sialidase_fam. IPR008377. Sialidase_trypan. IPR011040. Sialidases. [Graphical view]
PANTHER	PTHR10628. PTHR10628. 1 hit. PTHR10628:SF5. PTHR10628:SF5. 1 hit.
Pfam	PF02012. BNR. 3 hits. [Graphical view]
PRINTS	PR01803. TCSIALIDASE.
SUPFAM	SSF50939. Sialidase. 1 hit.
ProtoNet	Search...
Other
BindingDB	P10481.
ChEMBL	CHEMBL5189.

Entry information

Entry name

NANH_CLOPF

Accession

Primary (citable) accession number: P10481

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program