Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P10481 (NANH_CLOPE)

Last modified June 16, 2009. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Sialidase
    EC=3.2.1.18
Alternative name(s):
    Neuraminidase
Gene names
Name: nanH
OrganismClostridium perfringens
Taxonomic identifier1502 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length382 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Sialidases have been suggested to be pathogenic factors in microbial infections.

Catalytic activity

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Subcellular location

Secreted.

Sequence similarities

Belongs to the glycosyl hydrolase 33 family.

Contains 4 BNR repeats.

Ontologies

Keywords
   Cellular componentSecreted
   DomainRepeat
   Molecular functionGlycosidase
Hydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processmetabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

pathogenesis

Inferred from electronic annotation. Source: InterPro

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionexo-alpha-sialidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 382382Sialidase
PRO_0000208908

Regions

Repeat71 – 8212BNR 1
Repeat140 – 15112BNR 2
Repeat208 – 21912BNR 3
Repeat255 – 26612BNR 4

Sites

Active site621Proton acceptor By similarity
Active site3471Nucleophile By similarity
Binding site371Substrate By similarity
Binding site2451Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
P10481-1 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: D50857BB50A4E886

FASTA38242,813
        10         20         30         40         50         60 
MCNKNNTFEK NLDISHKPEP LILFNKDNNI WNSKYFRIPN IQLLNDGTIL TFSDIRYNGP 

        70         80         90        100        110        120 
DDHAYIDIAS ARSTDFGKTW SYNIAMKNNR IDSTYSRVMD STTVITNTGR IILIAGSWNT 

       130        140        150        160        170        180 
NGNWAMTTST RRSDWSVQMI YSDDNGLTWS NKIDLTKDSS KVKNQPSNTI GWLGGVGSGI 

       190        200        210        220        230        240 
VMDDGTIVMP AQISLRENNE NNYYSLIIYS KDNGETWTMG NKVPNSNTSE NMVIELDGAL 

       250        260        270        280        290        300 
IMSTRYDYSG YRAAYISHDL GTTWEIYEPL NGKILTGKGS GCQGSFIKAT TSNGHRIGLI 

       310        320        330        340        350        360 
SAPKNTKGEY IRDNIAVYMI DFDDLSKGVQ EICIPYPEDG NKLGGGYSCL SFKNNHLGIV 

       370        380 
YEANGNIEYQ DLTPYYSLIN KQ 

« Hide

References

[1]"Cloning and sequencing of a Clostridium perfringens sialidase gene."
Roggentin P., Rothe B., Lottspeich F., Schauer R.
FEBS Lett. 238:31-34(1988) [PubMed: 2901987] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 8-27.
Strain: A99.

Cross-references

Sequence databases

Y00963 Genomic DNA. Translation: CAA68780.1.
PIRS01339.

3D structure databases

HSSPHSSP built from PDB template 2SIL based on UniProtKB P29768.
ModBaseSearch...

Protein family/group databases

CAZyGH33. Glycoside Hydrolase Family 33.

Enzyme and pathway databases

BRENDA3.2.1.18. 2406.

Family and domain databases

InterProIPR008377. Sialidase_trypan.
[Graphical view]
PRINTSPR01803. TCSIALIDASE.
ProtoNetSearch...

Entry information

Entry nameNANH_CLOPE
AccessionPrimary (citable) accession number: P10481
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents