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P10478 (XYNZ_CLOTH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endo-1,4-beta-xylanase Z

Short name=Xylanase Z
EC=3.2.1.8
Alternative name(s):
1,4-beta-D-xylan xylanohydrolase Z
Gene names
Name:xynZ
Ordered Locus Names:Cthe_1963
OrganismClostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP]
Taxonomic identifier203119 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length837 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.

Domain

A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component.

Sequence similarities

Belongs to the glycosyl hydrolase 10 (cellulase F) family.

Contains 1 CBM6 (carbohydrate binding type-6) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Chain29 – 837809Endo-1,4-beta-xylanase Z
PRO_0000007973

Regions

Domain299 – 420122CBM6
Repeat430 – 453241
Repeat464 – 487242
Region430 – 487582 X 24 AA approximate repeats

Sites

Active site6451Proton donor
Active site7541Nucleophile

Amino acid modifications

Disulfide bond783 ↔ 789 By similarity

Secondary structure

................................................................................................ 837
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P10478 [UniParc].

Last modified November 1, 1991. Version 3.
Checksum: DD4C29F04D12B6CD

FASTA83792,263
        10         20         30         40         50         60 
MSRKLFSVLL VGLMLMTSLL VTISSTSAAS LPTMPPSGYD QVRNGVPRGQ VVNISYFSTA 

        70         80         90        100        110        120 
TNSTRPARVY LPPGYSKDKK YSVLYLLHGI GGSENDWFEG GGRANVIADN LIAEGKIKPL 

       130        140        150        160        170        180 
IIVTPNTNAA GPGIADGYEN FTKDLLNSLI PYIESNYSVY TDREHRAIAG LSMGGGQSFN 

       190        200        210        220        230        240 
IGLTNLDKFA YIGPISAAPN TYPNERLFPD GGKAAREKLK LLFIACGTND SLIGFGQRVH 

       250        260        270        280        290        300 
EYCVANNINH VYWLIQGGGH DFNVWKPGLW NFLQMADEAG LTRDGNTPVP TPSPKPANTR 

       310        320        330        340        350        360 
IEAEDYDGIN SSSIEIIGVP PEGGRGIGYI TSGDYLVYKS IDFGNGATSF KAKVANANTS 

       370        380        390        400        410        420 
NIELRLNGPN GTLIGTLSVK STGDWNTYEE QTCSISKVTG INDLYLVFKG PVNIDWFTFG 

       430        440        450        460        470        480 
VESSSTGLGD LNGDGNINSS DLQALKRHLL GISPLTGEAL LRADVNRSGK VDSTDYSVLK 

       490        500        510        520        530        540 
RYILRIITEF PGQGDVQTPN PSVTPTQTPI PTISGNALRD YAEARGIKIG TCVNYPFYNN 

       550        560        570        580        590        600 
SDPTYNSILQ REFSMVVCEN EMKFDALQPR QNVFDFSKGD QLLAFAERNG MQMRGHTLIW 

       610        620        630        640        650        660 
HNQNPSWLTN GNWNRDSLLA VMKNHITTVM THYKGKIVEW DVANECMDDS GNGLRSSIWR 

       670        680        690        700        710        720 
NVIGQDYLDY AFRYAREADP DALLFYNDYN IEDLGPKSNA VFNMIKSMKE RGVPIDGVGF 

       730        740        750        760        770        780 
QCHFINGMSP EYLASIDQNI KRYAEIGVIV SFTEIDIRIP QSENPATAFQ VQANNYKELM 

       790        800        810        820        830 
KICLANPNCN TFVMWGFTDK YTWIPGTFPG YGNPLIYDSN YNPKPAYNAI KEALMGY 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence and deletion analysis of the xylanase gene (xynZ) of Clostridium thermocellum."
Grepinet O., Chebrou M.-C., Beguin P.
J. Bacteriol. 170:4582-4588(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete sequence of Clostridium thermocellum ATCC 27405."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., Newcomb M., Richardson P.
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27405 / DSM 1237.
[3]"A common protein fold and similar active site in two distinct families of beta-glycanases."
Dominguez R., Souchon H., Spinelli S., Dauter Z., Wilson K.S., Chauvaux S., Beguin P., Alzari P.M.
Nat. Struct. Biol. 2:569-576(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) OF 515-837.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M22624 Genomic DNA. Translation: AAA23286.1.
CP000568 Genomic DNA. Translation: ABN53181.1.
PIRA31842.
RefSeqYP_001038374.1. NC_009012.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1JJFX-ray1.75A20-287[»]
1JT2X-ray1.80A20-287[»]
1XYZX-ray1.40A/B491-837[»]
ProteinModelPortalP10478.
SMRP10478. Positions 30-284, 299-419, 516-835.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP10478. 1 interaction.
STRING203119.Cthe_1963.

Protein family/group databases

CAZyCBM6. Carbohydrate-Binding Module Family 6.
GH10. Glycoside Hydrolase Family 10.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN53181; ABN53181; Cthe_1963.
GeneID4810746.
KEGGcth:Cthe_1963.
PATRIC19517811. VBICloThe47081_2079.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3693.
KOK01181.
OMAFRYAREA.
OrthoDBEOG6XQ3JG.

Enzyme and pathway databases

BioCycCTHE203119:GIW8-2026-MONOMER.
MetaCyc:MONOMER-16453.
BRENDA3.1.1.73. 1530.

Family and domain databases

Gene3D1.10.1330.10. 1 hit.
2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
3.40.50.1820. 1 hit.
InterProIPR029058. AB_hydrolase.
IPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR006584. Cellulose-bd_IV.
IPR005084. CMB_fam6.
IPR018242. Dockerin_1.
IPR018247. EF_Hand_1_Ca_BS.
IPR000801. Esterase_put.
IPR008979. Galactose-bd-like.
IPR001000. Glyco_hydro_10.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF03422. CBM_6. 1 hit.
PF00404. Dockerin_1. 2 hits.
PF00756. Esterase. 1 hit.
PF00331. Glyco_hydro_10. 1 hit.
[Graphical view]
PRINTSPR00134. GLHYDRLASE10.
SMARTSM00606. CBD_IV. 1 hit.
SM00633. Glyco_10. 1 hit.
[Graphical view]
SUPFAMSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF53474. SSF53474. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEPS51175. CBM6. 1 hit.
PS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS00018. EF_HAND_1. 2 hits.
PS00591. GLYCOSYL_HYDROL_F10. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP10478.

Entry information

Entry nameXYNZ_CLOTH
AccessionPrimary (citable) accession number: P10478
Secondary accession number(s): A3DGV2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1991
Last modified: June 11, 2014
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries