Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P10477 (GUNE_CLOTM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase E

EC=3.2.1.4
Alternative name(s):
Cellulase E
Endo-1,4-beta-glucanase E
Short name=EgE
Gene names
Name:celE
OrganismClostridium thermocellum
Taxonomic identifier1515 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length814 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Domain

A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component.

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3434 Ref.1
Chain35 – 814780Endoglucanase E
PRO_0000007852

Regions

Repeat415 – 438241
Repeat451 – 474242
Region415 – 474602 X 24 AA approximate repeats

Sites

Active site1931Proton donor By similarity
Active site3161Nucleophile By similarity

Secondary structure

.................................................................................................................... 814
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P10477 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: C6FA24B8D1523632

FASTA81490,244
        10         20         30         40         50         60 
MKKIVSLVCV LVMLVSILGS FSVVAASPVK GFQVSGTKLL DASGNELVMR GMRDISAIDL 

        70         80         90        100        110        120 
VKEIKIGWNL GNTLDAPTET AWGNPRTTKA MIEKVREMGF NAVRVPVTWD THIGPAPDYK 

       130        140        150        160        170        180 
IDEAWLNRVE EVVNYVLDCG MYAIINLHHD NTWIIPTYAN EQRSKEKLVK VWEQIATRFK 

       190        200        210        220        230        240 
DYDDHLLFET MNEPREVGSP MEWMGGTYEN RDVINRFNLA VVNTIRASGG NNDKRFILVP 

       250        260        270        280        290        300 
TNAATGLDVA LNDLVIPNND SRVIVSIHAY SPYFFAMDVN GTSYWGSDYD KASLTSELDA 

       310        320        330        340        350        360 
IYNRFVKNGR AVIIGEFGTI DKNNLSSRVA HAEHYAREAV SRGIAVFWWD NGYYNPGDAE 

       370        380        390        400        410        420 
TYALLNRKTL SWYYPEIVQA LMRGAGVEPL VSPTPTPTLM PTPSPTVTAN ILYGDVNGDG 

       430        440        450        460        470        480 
KINSTDCTML KRYILRGIEE FPSPSGIIAA DVNADLKINS TDLVLMKKYL LRSIDKFPAE 

       490        500        510        520        530        540 
DSQTPDEDNP GILYNGRFDF SDPNGPKCAW SGSNVELNFY GTEASVTIKS GGENWFQAIV 

       550        560        570        580        590        600 
DGNPLPPFSV NATTSTVKLV SGLAEGAHHL VLWKRTEASL GEVQFLGFDF GSGKLLAAPK 

       610        620        630        640        650        660 
PLERKIEFIG DSITCAYGNE GTSKEQSFTP KNENSYMSYA AITARNLNAS ANMIAWSGIG 

       670        680        690        700        710        720 
LTMNYGGAPG PLIMDRYPYT LPYSGVRWDF SKYVPQVVVI NLGTNDFSTS FADKTKFVTA 

       730        740        750        760        770        780 
YKNLISEVRR NYPDAHIFCC VGPMLWGTGL DLCRSYVTEV VNDCNRSGDL KVYFVEFPQQ 

       790        800        810 
DGSTGYGEDW HPSIATHQLM AERLTAEIKN KLGW 

« Hide

References

[1]"Conserved reiterated domains in Clostridium thermocellum endoglucanases are not essential for catalytic activity."
Hall J., Hazlewood G.P., Barker P.J., Gilbert H.J.
Gene 69:29-38(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 35-58.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M22759 Genomic DNA. Translation: AAA23224.1.
PIRCZCLEM. JT0347.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2WABX-ray1.90A485-814[»]
2WAOX-ray1.80A485-814[»]
4IM4X-ray2.42A/B/C/D/E/F51-386[»]
ProteinModelPortalP10477.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.1330.10. 1 hit.
3.20.20.80. 1 hit.
3.40.50.1110. 1 hit.
InterProIPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR018242. Dockerin_1.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR001087. Lipase_GDSL.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view]
PfamPF00150. Cellulase. 1 hit.
PF00404. Dockerin_1. 2 hits.
PF00657. Lipase_GDSL. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP10477.

Entry information

Entry nameGUNE_CLOTM
AccessionPrimary (citable) accession number: P10477
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries