Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P10476

- GUNA_CELJU

UniProt

P10476 - GUNA_CELJU

Protein

Endoglucanase A

Gene

celA

Organism
Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (20 Apr 2010)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei523 – 5231By similarity
    Active sitei573 – 5731By similarity
    Active sitei582 – 5821By similarity

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC
    2. cellulose binding Source: InterPro

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciCJAP498211:GHIT-2463-MONOMER.

    Protein family/group databases

    CAZyiCBM10. Carbohydrate-Binding Module Family 10.
    CBM2. Carbohydrate-Binding Module Family 2.
    GH9. Glycoside Hydrolase Family 9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase A (EC:3.2.1.4)
    Short name:
    EGA
    Alternative name(s):
    Cellulase
    Endo-1,4-beta-glucanase
    Gene namesi
    Name:celA
    Synonyms:cel9A
    Ordered Locus Names:CJA_2472
    OrganismiCellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
    Taxonomic identifieri498211 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeCellvibrio
    ProteomesiUP000001036: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232Sequence AnalysisAdd
    BLAST
    Chaini33 – 962930Endoglucanase APRO_0000007957Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi671 ↔ 702By similarity
    Disulfide bondi681 ↔ 696By similarity
    Disulfide bondi866 ↔ 961By similarity

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi498211.CJA_2472.

    Structurei

    3D structure databases

    ProteinModelPortaliP10476.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini671 – 69727CBM10Add
    BLAST
    Domaini859 – 962104CBM2Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi608 – 66457Ser-richAdd
    BLAST
    Compositional biasi823 – 85937Ser-rich (linker)Add
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOGENOMiHOG000245359.
    OrthoDBiEOG6QRW4D.

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    2.30.32.30. 1 hit.
    2.60.40.10. 1 hit.
    2.60.40.290. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR008965. Carb-bd_dom.
    IPR012291. CBD_carb-bd_dom.
    IPR002883. CBM10/Dockerin_dom.
    IPR018366. CBM2_CS.
    IPR009031. CBM_fam10.
    IPR001919. Cellulose-bd_dom_fam2_bac.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    IPR004197. Glyco_hydro_9_Ig-like.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR000601. PKD_dom.
    [Graphical view]
    PfamiPF02013. CBM_10. 1 hit.
    PF00553. CBM_2. 1 hit.
    PF02927. CelD_N. 1 hit.
    PF00759. Glyco_hydro_9. 1 hit.
    PF00801. PKD. 1 hit.
    [Graphical view]
    SMARTiSM00637. CBD_II. 1 hit.
    SM01064. CBM_10. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.
    SSF49299. SSF49299. 1 hit.
    SSF49384. SSF49384. 1 hit.
    SSF57615. SSF57615. 1 hit.
    SSF81296. SSF81296. 1 hit.
    PROSITEiPS51173. CBM2. 1 hit.
    PS00561. CBM2_A. 1 hit.
    PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
    PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P10476-1 [UniParc]FASTAAdd to Basket

    « Hide

    MINRSVLKIP ALVKPLVQAL VLVGCTLGVA QAEVGNPRVN QLGYIPNGDR    50
    IAVYKASNNS AQTWQLTHNG SLIASGQTIP KGSDASSGDN IHHIDLSSVT 100
    ATGSGFTLTV GGDSSYPFSI SSTTFNAAFY DALKYFYHNR SGIAIETPYT 150
    GGGRGSYASH SRWSRPAGHL NQGANKGDMN VPCWSGTCNY SLNVTKGWYD 200
    AGDHGKYVVN GGISVWTLLN LYERAQHITG NLAAVADGSM NIPESGNGVA 250
    DILDEARWQM EFMLAMQVPQ GQAKAGMAHH KIHDVGWTGL PLAPHEDPQQ 300
    RALVPPSTAA TLNLAATAAQ AARIWKDIDA GFAALCLTAA ERAWNAAQAN 350
    PNDIYSGNYD NGGGGYGDRF VADEFYWAAA ELYITTGDSR YLPTINNYTL 400
    ERTDFGWPDT ELLGVMSLAV VPATHTNSLR IAARNHIQTI ASTHLTTQSA 450
    SGYPAPLSSL EYYWGSNSVI ANKLVLMGLA YDFSGNQNFA LGVSKGINYL 500
    FGSNVLSTSF ITGLGTNTVA QPHHRFWAGA LNSNYPWAPP GALSGGPNAG 550
    LEDSLSASRL SGCTSRPATC WLDSIDAWST NEITINWNAP LAWVLGFYND 600
    FAATQGGSSS SSSSSSSSVP VSSSSSSSII PSSSSSSIQP SSSSSSMPSS 650
    SSSSSSVVAS SSSSVSGGLR CNWYGTLYPL CVTTQSGWGW ENSQSCISAS 700
    TCSAQPAPYG IVGAASSSSQ AANRSPTLQL SANATGFEGG SMVCCTLHIN 750
    GAASDPDGDN LTYSWQVISG NTVVASGSSS SASIHVSNQR GYEVSMTVSD 800
    GRGGVATETT FVSVYFSDYF PGSSSSASNI NSSSSSSSSS SSSAIVSSSS 850
    SVVSSSSSSA ASGGNCQYVV TNQWNNGFTA VIRVRNNGSS AINGWSVNWS 900
    YSDGSRITNS WNANVTGNNP YAASALGWNA NIQPGQTAEF GFQGTKGAGS 950
    AQVPAVTGSV CQ 962
    Length:962
    Mass (Da):100,109
    Last modified:April 20, 2010 - v2
    Checksum:i95212655950CB52A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti14 – 141K → T in CAA31082. (PubMed:2851699)Curated
    Sequence conflicti25 – 251C → G in CAA31082. (PubMed:2851699)Curated
    Sequence conflicti503 – 5031S → I in CAA31082. (PubMed:2851699)Curated
    Sequence conflicti507 – 5071S → P in CAA31082. (PubMed:2851699)Curated
    Sequence conflicti555 – 5551L → F in CAA31082. (PubMed:2851699)Curated
    Sequence conflicti894 – 8941G → R in CAA31082. (PubMed:2851699)Curated
    Sequence conflicti951 – 9511A → R in CAA31082. (PubMed:2851699)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12570 Genomic DNA. Translation: CAA31082.1.
    CP000934 Genomic DNA. Translation: ACE85757.1.
    RefSeqiWP_012488069.1. NC_010995.1.
    YP_001982933.1. NC_010995.1.

    Genome annotation databases

    EnsemblBacteriaiACE85757; ACE85757; CJA_2472.
    GeneIDi6416717.
    KEGGicja:CJA_2472.
    PATRICi21328310. VBICelJap122165_2424.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12570 Genomic DNA. Translation: CAA31082.1 .
    CP000934 Genomic DNA. Translation: ACE85757.1 .
    RefSeqi WP_012488069.1. NC_010995.1.
    YP_001982933.1. NC_010995.1.

    3D structure databases

    ProteinModelPortali P10476.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 498211.CJA_2472.

    Protein family/group databases

    CAZyi CBM10. Carbohydrate-Binding Module Family 10.
    CBM2. Carbohydrate-Binding Module Family 2.
    GH9. Glycoside Hydrolase Family 9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACE85757 ; ACE85757 ; CJA_2472 .
    GeneIDi 6416717.
    KEGGi cja:CJA_2472.
    PATRICi 21328310. VBICelJap122165_2424.

    Phylogenomic databases

    HOGENOMi HOG000245359.
    OrthoDBi EOG6QRW4D.

    Enzyme and pathway databases

    BioCyci CJAP498211:GHIT-2463-MONOMER.

    Family and domain databases

    Gene3Di 1.50.10.10. 1 hit.
    2.30.32.30. 1 hit.
    2.60.40.10. 1 hit.
    2.60.40.290. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR008965. Carb-bd_dom.
    IPR012291. CBD_carb-bd_dom.
    IPR002883. CBM10/Dockerin_dom.
    IPR018366. CBM2_CS.
    IPR009031. CBM_fam10.
    IPR001919. Cellulose-bd_dom_fam2_bac.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    IPR004197. Glyco_hydro_9_Ig-like.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR000601. PKD_dom.
    [Graphical view ]
    Pfami PF02013. CBM_10. 1 hit.
    PF00553. CBM_2. 1 hit.
    PF02927. CelD_N. 1 hit.
    PF00759. Glyco_hydro_9. 1 hit.
    PF00801. PKD. 1 hit.
    [Graphical view ]
    SMARTi SM00637. CBD_II. 1 hit.
    SM01064. CBM_10. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 1 hit.
    SSF49299. SSF49299. 1 hit.
    SSF49384. SSF49384. 1 hit.
    SSF57615. SSF57615. 1 hit.
    SSF81296. SSF81296. 1 hit.
    PROSITEi PS51173. CBM2. 1 hit.
    PS00561. CBM2_A. 1 hit.
    PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
    PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of a carboxymethylcellulase gene from Pseudomonas fluorescens subsp. cellulosa."
      Hall J., Gilbert H.J.
      Mol. Gen. Genet. 213:112-117(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus."
      DeBoy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E.
      J. Bacteriol. 190:5455-5463(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ueda107.

    Entry informationi

    Entry nameiGUNA_CELJU
    AccessioniPrimary (citable) accession number: P10476
    Secondary accession number(s): B3PKK4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: April 20, 2010
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3