P10476 (GUNA_CELJU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase A
Short name=EGA
EC=3.2.1.4

Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase

Gene names

Name:	celA
Synonyms:	cel9A
Ordered Locus Names:	CJA_2472

Organism

Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) [Complete proteome] [HAMAP]

Taxonomic identifier

498211 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Cellvibrio

Protein attributes

Sequence length	962 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family. Contains 1 CBM2 (carbohydrate binding type-2) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC polysaccharide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Potential
	Chain	33 – 962	930	Endoglucanase A		PRO_0000007957
Regions
	Domain	859 – 962	104	CBM2
	Compositional bias	608 – 664	57	Ser-rich
	Compositional bias	823 – 859	37	Ser-rich (linker)
Sites
	Active site	523	1	By similarity
	Active site	573	1	By similarity
	Active site	582	1	By similarity
Amino acid modifications
	Disulfide bond	671 ↔ 702		By similarity
	Disulfide bond	681 ↔ 696		By similarity
	Disulfide bond	866 ↔ 961		By similarity
Experimental info
	Sequence conflict	14	1	K → T in CAA31082. Ref.1
	Sequence conflict	25	1	C → G in CAA31082. Ref.1
	Sequence conflict	503	1	S → I in CAA31082. Ref.1
	Sequence conflict	507	1	S → P in CAA31082. Ref.1
	Sequence conflict	555	1	L → F in CAA31082. Ref.1
	Sequence conflict	894	1	G → R in CAA31082. Ref.1
	Sequence conflict	951	1	A → R in CAA31082. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P10476 [UniParc]. Last modified April 20, 2010. Version 2. Checksum: 95212655950CB52A	FASTA	962	100,109
	10 20 30 40 50 60 MINRSVLKIP ALVKPLVQAL VLVGCTLGVA QAEVGNPRVN QLGYIPNGDR IAVYKASNNS 70 80 90 100 110 120 AQTWQLTHNG SLIASGQTIP KGSDASSGDN IHHIDLSSVT ATGSGFTLTV GGDSSYPFSI 130 140 150 160 170 180 SSTTFNAAFY DALKYFYHNR SGIAIETPYT GGGRGSYASH SRWSRPAGHL NQGANKGDMN 190 200 210 220 230 240 VPCWSGTCNY SLNVTKGWYD AGDHGKYVVN GGISVWTLLN LYERAQHITG NLAAVADGSM 250 260 270 280 290 300 NIPESGNGVA DILDEARWQM EFMLAMQVPQ GQAKAGMAHH KIHDVGWTGL PLAPHEDPQQ 310 320 330 340 350 360 RALVPPSTAA TLNLAATAAQ AARIWKDIDA GFAALCLTAA ERAWNAAQAN PNDIYSGNYD 370 380 390 400 410 420 NGGGGYGDRF VADEFYWAAA ELYITTGDSR YLPTINNYTL ERTDFGWPDT ELLGVMSLAV 430 440 450 460 470 480 VPATHTNSLR IAARNHIQTI ASTHLTTQSA SGYPAPLSSL EYYWGSNSVI ANKLVLMGLA 490 500 510 520 530 540 YDFSGNQNFA LGVSKGINYL FGSNVLSTSF ITGLGTNTVA QPHHRFWAGA LNSNYPWAPP 550 560 570 580 590 600 GALSGGPNAG LEDSLSASRL SGCTSRPATC WLDSIDAWST NEITINWNAP LAWVLGFYND 610 620 630 640 650 660 FAATQGGSSS SSSSSSSSVP VSSSSSSSII PSSSSSSIQP SSSSSSMPSS SSSSSSVVAS 670 680 690 700 710 720 SSSSVSGGLR CNWYGTLYPL CVTTQSGWGW ENSQSCISAS TCSAQPAPYG IVGAASSSSQ 730 740 750 760 770 780 AANRSPTLQL SANATGFEGG SMVCCTLHIN GAASDPDGDN LTYSWQVISG NTVVASGSSS 790 800 810 820 830 840 SASIHVSNQR GYEVSMTVSD GRGGVATETT FVSVYFSDYF PGSSSSASNI NSSSSSSSSS 850 860 870 880 890 900 SSSAIVSSSS SVVSSSSSSA ASGGNCQYVV TNQWNNGFTA VIRVRNNGSS AINGWSVNWS 910 920 930 940 950 960 YSDGSRITNS WNANVTGNNP YAASALGWNA NIQPGQTAEF GFQGTKGAGS AQVPAVTGSV CQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of a carboxymethylcellulase gene from Pseudomonas fluorescens subsp. cellulosa." Hall J., Gilbert H.J. Mol. Gen. Genet. 213:112-117(1988) [PubMed: 2851699] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Insights into plant cell wall degradation from the genome sequence of the soil bacterium Cellvibrio japonicus." DeBoy R.T., Mongodin E.F., Fouts D.E., Tailford L.E., Khouri H., Emerson J.B., Mohamoud Y., Watkins K., Henrissat B., Gilbert H.J., Nelson K.E. J. Bacteriol. 190:5455-5463(2008) [PubMed: 18556790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ueda107.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X12570 Genomic DNA. Translation: CAA31082.1. CP000934 Genomic DNA. Translation: ACE85757.1.
RefSeq	YP_001982933.1. NC_010995.1.
3D structure databases
ProteinModelPortal	P10476.
ModBase	Search...
Protein family/group databases
CAZy	CBM10. Carbohydrate-Binding Module Family 10. CBM2. Carbohydrate-Binding Module Family 2. GH9. Glycoside Hydrolase Family 9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6416717.
GenomeReviews	Gene locus CJA_2472 in contig CP000934_GR.
KEGG	cja:CJA_2472.
PATRIC	21328310. VBICelJap122165_2424.
Organism-specific databases
CMR	Search...
Phylogenomic databases
ProtClustDB	CLSK2312845.
Family and domain databases
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR008965. Carb-bd_dom. IPR012291. CBD_carb-bd_dom. IPR002883. CBM10/Dockerin_dom. IPR018366. CBM2_CS. IPR009031. CBM_fam10. IPR001919. Cellulose-bd_dom_fam2_bac. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. IPR004197. Glyco_hydro_9_Ig-like. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR000601. PKD_dom. [Graphical view]
Gene3D	G3DSA:2.60.40.290. CBD_carb_bd. 1 hit. G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit. G3DSA:2.30.32.30. TypeX_cellulose-bd_reg_CBDX. 1 hit.
Pfam	PF02013. CBM_10. 1 hit. PF00553. CBM_2. 1 hit. PF02927. CelD_N. 1 hit. PF00759. Glyco_hydro_9. 1 hit. PF00801. PKD. 1 hit. [Graphical view]
SMART	SM00637. CBD_II. 1 hit. SM01064. CBM_10. 1 hit. [Graphical view]
SUPFAM	SSF57615. CBDX. 1 hit. SSF49384. Cellul_bind. 1 hit. SSF48208. Glyco_trans_6hp. 1 hit. SSF81296. Ig_E-set. 1 hit. SSF49299. PKD. 1 hit.
PROSITE	PS51173. CBM2. 1 hit. PS00561. CBM2_A. 1 hit. PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUNA_CELJU

Accession

Primary (citable) accession number: P10476
Secondary accession number(s): B3PKK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	April 20, 2010
Last modified:	January 25, 2012
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program