Reviewed, UniProtKB/Swiss-Prot P10476 (GUNA_PSEFL)

Names and origin

Protein names

Recommended name:
    Endoglucanase A
      Short name=EGA
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta-glucanase
    Cellulase

Gene names

Name:

celA

Organism

Pseudomonas fluorescens

Taxonomic identifier

294 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	962 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family. Contains 1 CBM2 (carbohydrate binding type-2) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC polysaccharide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Potential
	Chain	33 – 962	930	Endoglucanase A		PRO_0000007957
Regions
	Domain	859 – 962	104	CBM2
	Compositional bias	608 – 664	57	Ser-rich
	Compositional bias	823 – 859	37	Ser-rich (linker)
Sites
	Active site	523	1	By similarity
	Active site	573	1	By similarity
	Active site	582	1	By similarity
Amino acid modifications
	Disulfide bond	671 ↔ 702		By similarity
	Disulfide bond	681 ↔ 696		By similarity
	Disulfide bond	866 ↔ 961		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P10476-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 9E93FE1EAC21A898	FASTA	962	100,290
	10 20 30 40 50 60 MINRSVLKIP ALVTPLVQAL VLVGGTLGVA QAEVGNPRVN QLGYIPNGDR IAVYKASNNS 70 80 90 100 110 120 AQTWQLTHNG SLIASGQTIP KGSDASSGDN IHHIDLSSVT ATGSGFTLTV GGDSSYPFSI 130 140 150 160 170 180 SSTTFNAAFY DALKYFYHNR SGIAIETPYT GGGRGSYASH SRWSRPAGHL NQGANKGDMN 190 200 210 220 230 240 VPCWSGTCNY SLNVTKGWYD AGDHGKYVVN GGISVWTLLN LYERAQHITG NLAAVADGSM 250 260 270 280 290 300 NIPESGNGVA DILDEARWQM EFMLAMQVPQ GQAKAGMAHH KIHDVGWTGL PLAPHEDPQQ 310 320 330 340 350 360 RALVPPSTAA TLNLAATAAQ AARIWKDIDA GFAALCLTAA ERAWNAAQAN PNDIYSGNYD 370 380 390 400 410 420 NGGGGYGDRF VADEFYWAAA ELYITTGDSR YLPTINNYTL ERTDFGWPDT ELLGVMSLAV 430 440 450 460 470 480 VPATHTNSLR IAARNHIQTI ASTHLTTQSA SGYPAPLSSL EYYWGSNSVI ANKLVLMGLA 490 500 510 520 530 540 YDFSGNQNFA LGVSKGINYL FGINVLPTSF ITGLGTNTVA QPHHRFWAGA LNSNYPWAPP 550 560 570 580 590 600 GALSGGPNAG LEDSFSASRL SGCTSRPATC WLDSIDAWST NEITINWNAP LAWVLGFYND 610 620 630 640 650 660 FAATQGGSSS SSSSSSSSVP VSSSSSSSII PSSSSSSIQP SSSSSSMPSS SSSSSSVVAS 670 680 690 700 710 720 SSSSVSGGLR CNWYGTLYPL CVTTQSGWGW ENSQSCISAS TCSAQPAPYG IVGAASSSSQ 730 740 750 760 770 780 AANRSPTLQL SANATGFEGG SMVCCTLHIN GAASDPDGDN LTYSWQVISG NTVVASGSSS 790 800 810 820 830 840 SASIHVSNQR GYEVSMTVSD GRGGVATETT FVSVYFSDYF PGSSSSASNI NSSSSSSSSS 850 860 870 880 890 900 SSSAIVSSSS SVVSSSSSSA ASGGNCQYVV TNQWNNGFTA VIRVRNNGSS AINRWSVNWS 910 920 930 940 950 960 YSDGSRITNS WNANVTGNNP YAASALGWNA NIQPGQTAEF GFQGTKGAGS RQVPAVTGSV CQ

References

[1]	"The nucleotide sequence of a carboxymethylcellulase gene from Pseudomonas fluorescens subsp. cellulosa." Hall J., Gilbert H.J. Mol. Gen. Genet. 213:112-117(1988) [PubMed: 2851699] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sp. Cellulosa.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X12570 Genomic DNA. Translation: CAA31082.1.
3D structure databases
HSSP	HSSP built from PDB template 1E8R based on UniProtKB P14768.
ModBase	Search...
Protein family/group databases
CAZy	CBM10. Carbohydrate-Binding Module Family 10. CBM2. Carbohydrate-Binding Module Family 2. GH9. Glycoside Hydrolase Family 9.
Enzyme and pathway databases
BRENDA	3.2.1.4. 329.
Family and domain databases
InterPro	IPR012341. 6hp_glycosidase. IPR001919. CBD_bac. IPR012291. CBD_carb_bd. IPR018366. CBM2_CS. IPR002883. Dockerin_CBD_5. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. IPR004197. Glyco_hydro_9_Ig-like. IPR013783. Ig-like_fold. IPR000601. PKD. IPR009031. TypeX_cellulose-bd_reg_CBDX. [Graphical view]
Gene3D	G3DSA:2.60.40.290. CBD_carb_bd. 1 hit. G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit. G3DSA:2.30.32.30. TypeX_cellulose-bd_reg_CBDX. 1 hit.
PANTHER	PTHR22298:SF3. Glyco_hydro_9. 1 hit.
Pfam	PF02013. CBM_10. 1 hit. PF00553. CBM_2. 1 hit. PF02927. CelD_N. 1 hit. PF00759. Glyco_hydro_9. 1 hit. PF00801. PKD. 1 hit. [Graphical view]
SMART	SM00637. CBD_II. 1 hit. [Graphical view]
PROSITE	PS51173. CBM2. 1 hit. PS00561. CBM2_A. 1 hit. PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUNA_PSEFL

Accession

Primary (citable) accession number: P10476

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	June 16, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)