ID   GUN2_BACSU              Reviewed;         499 AA.
AC   P10475;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   27-MAR-2024, entry version 167.
DE   RecName: Full=Endoglucanase;
DE            EC=3.2.1.4;
DE   AltName: Full=Carboxymethyl-cellulase;
DE            Short=CMCase;
DE            Short=Cellulase;
DE   AltName: Full=Endo-1,4-beta-glucanase;
DE   Flags: Precursor;
GN   Name=eglS; Synonyms=bglC, gld; OrderedLocusNames=BSU18130;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PAP115;
RX   PubMed=3024130; DOI=10.1093/nar/14.22.9159;
RA   Mackay R.M., Lo A., Willick G., Zuker M., Baird S., Dove M., Moranelli F.,
RA   Seligy V.;
RT   "Structure of a Bacillus subtilis endo-beta-1,4-glucanase gene.";
RL   Nucleic Acids Res. 14:9159-9170(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CK-2;
RX   PubMed=7710280; DOI=10.1007/bf00882768;
RA   Lindahl V., Aa K., Tronsmo A.;
RT   "Nucleotide sequence of an endo-beta-1,4-glucanase gene from Bacillus
RT   subtilis CK-2.";
RL   Antonie Van Leeuwenhoek 66:327-332(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969507; DOI=10.1099/13500872-142-11-3097;
RA   Rose M., Entian K.-D.;
RT   "New genes in the 170 degrees region of the Bacillus subtilis genome encode
RT   DNA gyrase subunits, a thioredoxin, a xylanase and an amino acid
RT   transporter.";
RL   Microbiology 142:3097-3101(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [5]
RP   PROTEIN SEQUENCE OF 30-45.
RC   STRAIN=CK-2;
RX   PubMed=7710279; DOI=10.1007/bf00882767;
RA   Aa K., Flengsrud R., Lindahl V., Tronsmo A.;
RT   "Characterization of production and enzyme properties of an endo-beta-1,4-
RT   glucanase from Bacillus subtilis CK-2 isolated from compost soil.";
RL   Antonie Van Leeuwenhoek 66:319-326(1994).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA97610.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; Z29076; CAA82317.1; -; Genomic_DNA.
DR   EMBL; X04689; CAA28392.1; -; Genomic_DNA.
DR   EMBL; X67044; CAA47429.1; -; Genomic_DNA.
DR   EMBL; Z73234; CAA97610.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AL009126; CAB13696.2; -; Genomic_DNA.
DR   PIR; G69593; G69593.
DR   RefSeq; NP_389695.2; NC_000964.3.
DR   RefSeq; WP_003231540.1; NZ_JNCM01000035.1.
DR   PDB; 2L8A; NMR; -; A=354-499.
DR   PDB; 3PZT; X-ray; 1.97 A; A/B=27-332.
DR   PDB; 3PZU; X-ray; 2.10 A; A/B=27-332.
DR   PDB; 3PZV; X-ray; 2.87 A; A/B/C/D=27-332.
DR   PDB; 6UFV; X-ray; 1.06 A; A=354-499.
DR   PDB; 6UFW; X-ray; 1.28 A; A=354-499.
DR   PDBsum; 2L8A; -.
DR   PDBsum; 3PZT; -.
DR   PDBsum; 3PZU; -.
DR   PDBsum; 3PZV; -.
DR   PDBsum; 6UFV; -.
DR   PDBsum; 6UFW; -.
DR   AlphaFoldDB; P10475; -.
DR   BMRB; P10475; -.
DR   SMR; P10475; -.
DR   STRING; 224308.BSU18130; -.
DR   CAZy; CBM3; Carbohydrate-Binding Module Family 3.
DR   CAZy; GH5; Glycoside Hydrolase Family 5.
DR   PaxDb; 224308-BSU18130; -.
DR   EnsemblBacteria; CAB13696; CAB13696; BSU_18130.
DR   GeneID; 938607; -.
DR   KEGG; bsu:BSU18130; -.
DR   PATRIC; fig|224308.179.peg.1977; -.
DR   eggNOG; COG2730; Bacteria.
DR   InParanoid; P10475; -.
DR   OrthoDB; 154460at2; -.
DR   BioCyc; BSUB:BSU18130-MONOMER; -.
DR   BRENDA; 3.2.1.4; 658.
DR   EvolutionaryTrace; P10475; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0009986; C:cell surface; IBA:GO_Central.
DR   GO; GO:0005576; C:extracellular region; IBA:GO_Central.
DR   GO; GO:0008422; F:beta-glucosidase activity; IBA:GO_Central.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030248; F:cellulose binding; IEA:InterPro.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0009251; P:glucan catabolic process; IBA:GO_Central.
DR   Gene3D; 2.60.40.710; Endoglucanase-like; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR008965; CBM2/CBM3_carb-bd_dom_sf.
DR   InterPro; IPR001956; CBM3.
DR   InterPro; IPR036966; CBM3_sf.
DR   InterPro; IPR001547; Glyco_hydro_5.
DR   InterPro; IPR018087; Glyco_hydro_5_CS.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR34142:SF1; CELLULASE DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR34142; ENDO-BETA-1,4-GLUCANASE A; 1.
DR   Pfam; PF00942; CBM_3; 1.
DR   Pfam; PF00150; Cellulase; 1.
DR   SMART; SM01067; CBM_3; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF49384; Carbohydrate-binding domain; 1.
DR   PROSITE; PS51172; CBM3; 1.
DR   PROSITE; PS00659; GLYCOSYL_HYDROL_F5; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Carbohydrate metabolism; Cellulose degradation;
KW   Direct protein sequencing; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Reference proteome; Signal.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000269|PubMed:7710279"
FT   CHAIN           30..499
FT                   /note="Endoglucanase"
FT                   /id="PRO_0000007840"
FT   DOMAIN          350..499
FT                   /note="CBM3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00513"
FT   ACT_SITE        169
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:O85465"
FT   ACT_SITE        257
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:O85465"
FT   BINDING         65
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:O85465"
FT   BINDING         69..70
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:O85465"
FT   BINDING         96
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:O85465"
FT   BINDING         131
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:O85465"
FT   BINDING         231
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:O85465"
FT   BINDING         263..264
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:O85465"
FT   BINDING         291
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:O85465"
FT   BINDING         296..298
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:O85465"
FT   CONFLICT        283
FT                   /note="S -> N (in Ref. 2; CAA47429)"
FT                   /evidence="ECO:0000305"
FT   HELIX           36..39
FT                   /evidence="ECO:0007829|PDB:3PZU"
FT   STRAND          43..45
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   STRAND          48..50
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   STRAND          60..65
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   HELIX           67..70
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   HELIX           71..73
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   HELIX           76..85
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   STRAND          89..98
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   TURN            102..104
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   HELIX           106..108
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   HELIX           109..122
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   STRAND          125..131
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   STRAND          133..135
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   TURN            138..141
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   HELIX           142..156
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   STRAND          162..165
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   TURN            176..179
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   HELIX           180..194
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   STRAND          196..198
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   STRAND          200..202
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   HELIX           205..208
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   HELIX           211..215
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   STRAND          224..231
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   TURN            232..234
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   HELIX           237..248
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   STRAND          253..261
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   HELIX           271..283
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   STRAND          288..294
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   HELIX           315..317
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   HELIX           320..330
FT                   /evidence="ECO:0007829|PDB:3PZT"
FT   STRAND          354..360
FT                   /evidence="ECO:0007829|PDB:6UFV"
FT   STRAND          372..378
FT                   /evidence="ECO:0007829|PDB:6UFV"
FT   STRAND          380..382
FT                   /evidence="ECO:0007829|PDB:6UFV"
FT   HELIX           386..388
FT                   /evidence="ECO:0007829|PDB:6UFV"
FT   STRAND          389..395
FT                   /evidence="ECO:0007829|PDB:6UFV"
FT   STRAND          402..409
FT                   /evidence="ECO:0007829|PDB:6UFV"
FT   HELIX           413..415
FT                   /evidence="ECO:0007829|PDB:6UFV"
FT   STRAND          416..427
FT                   /evidence="ECO:0007829|PDB:6UFV"
FT   STRAND          430..440
FT                   /evidence="ECO:0007829|PDB:6UFV"
FT   STRAND          447..450
FT                   /evidence="ECO:0007829|PDB:2L8A"
FT   STRAND          452..458
FT                   /evidence="ECO:0007829|PDB:6UFV"
FT   HELIX           466..468
FT                   /evidence="ECO:0007829|PDB:6UFV"
FT   STRAND          482..488
FT                   /evidence="ECO:0007829|PDB:6UFV"
FT   STRAND          491..495
FT                   /evidence="ECO:0007829|PDB:6UFV"
SQ   SEQUENCE   499 AA;  55287 MW;  8F735FF711B3EAE2 CRC64;
     MKRSISIFIT CLLITLLTMG GMIASPASAA GTKTPVAKNG QLSIKGTQLV NRDGKAVQLK
     GISSHGLQWY GEYVNKDSLK WLRDDWGITV FRAAMYTADG GYIDNPSVKN KVKEAVEAAK
     ELGIYVIIDW HILNDGNPNQ NKEKAKEFFK EMSSLYGNTP NVIYEIANEP NGDVNWKRDI
     KPYAEEVISV IRKNDPDNII IVGTGTWSQD VNDAADDQLK DANVMYALHF YAGTHGQFLR
     DKANYALSKG APIFVTEWGT SDASGNGGVF LDQSREWLKY LDSKTISWVN WNLSDKQESS
     SALKPGASKT GGWRLSDLSA SGTFVRENIL GTKDSTKDIP ETPSKDKPTQ ENGISVQYRA
     GDGSMNSNQI RPQLQIKNNG NTTVDLKDVT ARYWYKAKNK GQNFDCDYAQ IGCGNVTHKF
     VTLHKPKQGA DTYLELGFKN GTLAPGASTG NIQLRLHNDD WSNYAQSGDY SFFKSNTFKT
     TKKITLYDQG KLIWGTEPN
//