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Protein

Endoglucanase

Gene

eglS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei65SubstrateBy similarity1
Binding sitei96SubstrateBy similarity1
Binding sitei131SubstrateBy similarity1
Active sitei169Proton donorBy similarity1
Binding sitei231SubstrateBy similarity1
Active sitei257NucleophileBy similarity1
Binding sitei291SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciBSUB:BSU18130-MONOMER

Protein family/group databases

CAZyiCBM3 Carbohydrate-Binding Module Family 3
GH5 Glycoside Hydrolase Family 5

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase (EC:3.2.1.4)
Alternative name(s):
Carboxymethyl-cellulase
Short name:
CMCase
Short name:
Cellulase
Endo-1,4-beta-glucanase
Gene namesi
Name:eglS
Synonyms:bglC, gld
Ordered Locus Names:BSU18130
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 291 PublicationAdd BLAST29
ChainiPRO_000000784030 – 499EndoglucanaseAdd BLAST470

Proteomic databases

PaxDbiP10475
PRIDEiP10475

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100009986

Structurei

Secondary structure

1499
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi36 – 39Combined sources4
Beta strandi43 – 45Combined sources3
Beta strandi48 – 50Combined sources3
Beta strandi60 – 65Combined sources6
Helixi67 – 70Combined sources4
Helixi71 – 73Combined sources3
Helixi76 – 85Combined sources10
Beta strandi89 – 98Combined sources10
Turni102 – 104Combined sources3
Helixi106 – 108Combined sources3
Helixi109 – 122Combined sources14
Beta strandi125 – 131Combined sources7
Beta strandi133 – 135Combined sources3
Turni138 – 141Combined sources4
Helixi142 – 156Combined sources15
Beta strandi162 – 165Combined sources4
Turni176 – 179Combined sources4
Helixi180 – 194Combined sources15
Beta strandi196 – 198Combined sources3
Beta strandi200 – 202Combined sources3
Helixi205 – 208Combined sources4
Helixi211 – 215Combined sources5
Beta strandi224 – 231Combined sources8
Turni232 – 234Combined sources3
Helixi237 – 248Combined sources12
Beta strandi253 – 261Combined sources9
Helixi271 – 283Combined sources13
Beta strandi288 – 294Combined sources7
Helixi315 – 317Combined sources3
Helixi320 – 330Combined sources11
Beta strandi355 – 360Combined sources6
Beta strandi373 – 377Combined sources5
Helixi386 – 388Combined sources3
Beta strandi389 – 395Combined sources7
Beta strandi403 – 409Combined sources7
Beta strandi417 – 422Combined sources6
Beta strandi427 – 429Combined sources3
Beta strandi432 – 438Combined sources7
Beta strandi447 – 450Combined sources4
Beta strandi452 – 457Combined sources6
Turni466 – 473Combined sources8
Beta strandi484 – 488Combined sources5
Beta strandi491 – 494Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2L8ANMR-A354-499[»]
3PZTX-ray1.97A/B27-332[»]
3PZUX-ray2.10A/B27-332[»]
3PZVX-ray2.87A/B/C/D27-332[»]
ProteinModelPortaliP10475
SMRiP10475
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10475

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini350 – 499CBM3PROSITE-ProRule annotationAdd BLAST150

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni69 – 70Substrate bindingBy similarity2
Regioni263 – 264Substrate bindingBy similarity2
Regioni296 – 298Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QWR Bacteria
COG2730 LUCA
HOGENOMiHOG000253566
InParanoidiP10475
KOiK01179
OMAiNEPNGDV

Family and domain databases

Gene3Di2.60.40.710, 1 hit
InterProiView protein in InterPro
IPR008965 CBM2/CBM3_carb-bd_dom_sf
IPR001956 CBM3
IPR036966 CBM3_sf
IPR001547 Glyco_hydro_5
IPR018087 Glyco_hydro_5_CS
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00942 CBM_3, 1 hit
PF00150 Cellulase, 1 hit
SMARTiView protein in SMART
SM01067 CBM_3, 1 hit
SUPFAMiSSF49384 SSF49384, 1 hit
SSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS51172 CBM3, 1 hit
PS00659 GLYCOSYL_HYDROL_F5, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10475-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRSISIFIT CLLITLLTMG GMIASPASAA GTKTPVAKNG QLSIKGTQLV
60 70 80 90 100
NRDGKAVQLK GISSHGLQWY GEYVNKDSLK WLRDDWGITV FRAAMYTADG
110 120 130 140 150
GYIDNPSVKN KVKEAVEAAK ELGIYVIIDW HILNDGNPNQ NKEKAKEFFK
160 170 180 190 200
EMSSLYGNTP NVIYEIANEP NGDVNWKRDI KPYAEEVISV IRKNDPDNII
210 220 230 240 250
IVGTGTWSQD VNDAADDQLK DANVMYALHF YAGTHGQFLR DKANYALSKG
260 270 280 290 300
APIFVTEWGT SDASGNGGVF LDQSREWLKY LDSKTISWVN WNLSDKQESS
310 320 330 340 350
SALKPGASKT GGWRLSDLSA SGTFVRENIL GTKDSTKDIP ETPSKDKPTQ
360 370 380 390 400
ENGISVQYRA GDGSMNSNQI RPQLQIKNNG NTTVDLKDVT ARYWYKAKNK
410 420 430 440 450
GQNFDCDYAQ IGCGNVTHKF VTLHKPKQGA DTYLELGFKN GTLAPGASTG
460 470 480 490
NIQLRLHNDD WSNYAQSGDY SFFKSNTFKT TKKITLYDQG KLIWGTEPN
Length:499
Mass (Da):55,287
Last modified:July 1, 1989 - v1
Checksum:i8F735FF711B3EAE2
GO

Sequence cautioni

The sequence CAA97610 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti283S → N in CAA47429 (PubMed:7710280).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z29076 Genomic DNA Translation: CAA82317.1
X04689 Genomic DNA Translation: CAA28392.1
X67044 Genomic DNA Translation: CAA47429.1
Z73234 Genomic DNA Translation: CAA97610.1 Different initiation.
AL009126 Genomic DNA Translation: CAB13696.2
PIRiG69593
RefSeqiNP_389695.2, NC_000964.3
WP_003231540.1, NZ_JNCM01000035.1

Genome annotation databases

EnsemblBacteriaiCAB13696; CAB13696; BSU18130
GeneIDi938607
KEGGibsu:BSU18130
PATRICifig|224308.179.peg.1977

Similar proteinsi

Entry informationi

Entry nameiGUN2_BACSU
AccessioniPrimary (citable) accession number: P10475
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 23, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health