Reviewed, UniProtKB/Swiss-Prot P10475 (GUN2_BACSU)

Names and origin

Protein names

Recommended name:
    Endoglucanase
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta-glucanase
    Carboxymethyl-cellulase
      Short name=CMCase
      Short name=Cellulase

Gene names

Name:	eglS
Synonyms:	bglC, gld
Ordered Locus Names:	BSU18130

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family. Contains 1 CBM3 (carbohydrate binding type-3) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro cation binding Inferred from electronic annotation. Source: InterPro cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Ref.5
	Chain	30 – 499	470	Endoglucanase		PRO_0000007840
Regions
	Domain	350 – 499	150	CBM3
Sites
	Active site	169	1	Proton donor By similarity
	Active site	257	1	Nucleophile By similarity
Experimental info
	Sequence conflict	283	1	S → N in CAA47429. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P10475-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 8F735FF711B3EAE2	FASTA	499	55,287
	10 20 30 40 50 60 MKRSISIFIT CLLITLLTMG GMIASPASAA GTKTPVAKNG QLSIKGTQLV NRDGKAVQLK 70 80 90 100 110 120 GISSHGLQWY GEYVNKDSLK WLRDDWGITV FRAAMYTADG GYIDNPSVKN KVKEAVEAAK 130 140 150 160 170 180 ELGIYVIIDW HILNDGNPNQ NKEKAKEFFK EMSSLYGNTP NVIYEIANEP NGDVNWKRDI 190 200 210 220 230 240 KPYAEEVISV IRKNDPDNII IVGTGTWSQD VNDAADDQLK DANVMYALHF YAGTHGQFLR 250 260 270 280 290 300 DKANYALSKG APIFVTEWGT SDASGNGGVF LDQSREWLKY LDSKTISWVN WNLSDKQESS 310 320 330 340 350 360 SALKPGASKT GGWRLSDLSA SGTFVRENIL GTKDSTKDIP ETPSKDKPTQ ENGISVQYRA 370 380 390 400 410 420 GDGSMNSNQI RPQLQIKNNG NTTVDLKDVT ARYWYKAKNK GQNFDCDYAQ IGCGNVTHKF 430 440 450 460 470 480 VTLHKPKQGA DTYLELGFKN GTLAPGASTG NIQLRLHNDD WSNYAQSGDY SFFKSNTFKT 490 TKKITLYDQG KLIWGTEPN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of a Bacillus subtilis endo-beta-1,4-glucanase gene." Mackay R.M., Lo A., Willick G., Zuker M., Baird S., Dove M., Moranelli F., Seligy V. Nucleic Acids Res. 14:9159-9170(1986) [PubMed: 3024130] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PAP115.
[2]	"Nucleotide sequence of an endo-beta-1,4-glucanase gene from Bacillus subtilis CK-2." Lindahl V., Aa K., Tronsmo A. Antonie Van Leeuwenhoek 66:327-332(1994) [PubMed: 7710280] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CK-2.
[3]	"New genes in the 170 degrees region of the Bacillus subtilis genome encode DNA gyrase subunits, a thioredoxin, a xylanase and an amino acid transporter." Rose M., Entian K.-D. Microbiology 142:3097-3101(1996) [PubMed: 8969507] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[4]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[5]	"Characterization of production and enzyme properties of an endo-beta-1,4-glucanase from Bacillus subtilis CK-2 isolated from compost soil." Aa K., Flengsrud R., Lindahl V., Tronsmo A. Antonie Van Leeuwenhoek 66:319-326(1994) [PubMed: 7710279] [Abstract] Cited for: PROTEIN SEQUENCE OF 30-45. Strain: CK-2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z29076 Genomic DNA. Translation: CAA82317.1. X04689 Genomic DNA. Translation: CAA28392.1. X67044 Genomic DNA. Translation: CAA47429.1. Z73234 Genomic DNA. Translation: CAA97610.1. Different initiation. AL009126 Genomic DNA. Translation: CAB13696.2.
PIR	G69593.
RefSeq	NP_389695.2.
3D structure databases
HSSP	HSSP built from PDB template 1LF1 based on UniProtKB Q59232.
SMR	P10475. Positions 36-329.
ModBase	Search...
Protein family/group databases
CAZy	CBM3. Carbohydrate-Binding Module Family 3. GH5. Glycoside Hydrolase Family 5.
Genome annotation databases
GeneID	938607.
GenomeReviews	Gene locus BSU18130 in contig AL009126_GR.
KEGG	bsu:BSU18130.
NMPDR	fig|224308.1.peg.1816.
Organism-specific databases
SubtiList	BG10437. bglC. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P10475.
OMA	P10475. WLRDDWG.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU1813-MON.
BRENDA	3.2.1.4. 150.
Family and domain databases
InterPro	IPR001956. CBD_3. IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:2.60.40.710. CBD_3. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00942. CBM_3. 1 hit. PF00150. Cellulase. 1 hit. [Graphical view]
ProDom	PD001947. CBD_3. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS51172. CBM3. 1 hit. PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN2_BACSU

Accession

Primary (citable) accession number: P10475

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	June 16, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)