Reviewed,
UniProtKB/Swiss-Prot P10475 (GUN2_BACSU)
Last modified
June 16, 2009.
Version 91.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Endoglucanase EC=3.2.1.4 Alternative name(s): Endo-1,4-beta-glucanase Carboxymethyl-cellulase Short name=CMCase Short name=Cellulase | ||||||
| Gene names |
| ||||||
| Organism | Bacillus subtilis [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 1423 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus |
Protein attributes
| Sequence length | 499 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. |
| Sequence similarities | Belongs to the glycosyl hydrolase 5 (cellulase A) family. Contains 1 CBM3 (carbohydrate binding type-3) domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Carbohydrate metabolism Cellulose degradation Polysaccharide degradation |
| Domain | Signal |
| Molecular function | Glycosidase Hydrolase |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | carbohydrate binding Inferred from electronic annotation. Source: InterPro cation bindingInferred from electronic annotation. Source: InterPro cellulase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 29 | 29 | Ref.5 | ||||||
| Chain | 30 – 499 | 470 | Endoglucanase | PRO_0000007840 | |||||
Regions | |||||||||
| Domain | 350 – 499 | 150 | CBM3 | ||||||
Sites | |||||||||
| Active site | 169 | 1 | Proton donor By similarity | ||||||
| Active site | 257 | 1 | Nucleophile By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 283 | 1 | S → N in CAA47429. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Structure of a Bacillus subtilis endo-beta-1,4-glucanase gene." Mackay R.M., Lo A., Willick G., Zuker M., Baird S., Dove M., Moranelli F., Seligy V. Nucleic Acids Res. 14:9159-9170(1986) [PubMed: 3024130] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PAP115. |
| [2] | "Nucleotide sequence of an endo-beta-1,4-glucanase gene from Bacillus subtilis CK-2." Lindahl V., Aa K., Tronsmo A. Antonie Van Leeuwenhoek 66:327-332(1994) [PubMed: 7710280] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CK-2. |
| [3] | "New genes in the 170 degrees region of the Bacillus subtilis genome encode DNA gyrase subunits, a thioredoxin, a xylanase and an amino acid transporter." Rose M., Entian K.-D. Microbiology 142:3097-3101(1996) [PubMed: 8969507] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168. |
| [4] | "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. Danchin A.Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168. |
| [5] | "Characterization of production and enzyme properties of an endo-beta-1,4-glucanase from Bacillus subtilis CK-2 isolated from compost soil." Aa K., Flengsrud R., Lindahl V., Tronsmo A. Antonie Van Leeuwenhoek 66:319-326(1994) [PubMed: 7710279] [Abstract] Cited for: PROTEIN SEQUENCE OF 30-45. Strain: CK-2. |
Cross-references
Sequence databases | |
|---|---|
| Z29076 Genomic DNA. Translation: CAA82317.1. X04689 Genomic DNA. Translation: CAA28392.1. X67044 Genomic DNA. Translation: CAA47429.1. Z73234 Genomic DNA. Translation: CAA97610.1. Different initiation. AL009126 Genomic DNA. Translation: CAB13696.2. | |
| PIR | G69593. |
| RefSeq | NP_389695.2. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1LF1 based on UniProtKB Q59232. |
| SMR | P10475. Positions 36-329. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | CBM3. Carbohydrate-Binding Module Family 3. GH5. Glycoside Hydrolase Family 5. |
Genome annotation databases | |
| GeneID | 938607. |
| GenomeReviews | Gene locus BSU18130 in contig AL009126_GR. |
| KEGG | bsu:BSU18130. |
| NMPDR | fig|224308.1.peg.1816. |
Organism-specific databases | |
| SubtiList | BG10437. bglC. [Micado] |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P10475. |
| OMA | P10475. WLRDDWG. |
Enzyme and pathway databases | |
| BioCyc | BSUB224308:BSU1813-MON. |
| BRENDA | 3.2.1.4. 150. |
Family and domain databases | |
| InterPro | IPR001956. CBD_3. IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view] |
| Gene3D | G3DSA:2.60.40.710. CBD_3. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. |
| Pfam | PF00942. CBM_3. 1 hit. PF00150. Cellulase. 1 hit. [Graphical view] |
| ProDom | PD001947. CBD_3. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| PROSITE | PS51172. CBM3. 1 hit. PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | GUN2_BACSU | ||||||||
| Accession | Primary (citable) accession number: P10475 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Bacillus subtilis Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList |
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

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