Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P10474

- GUNB_CALSA

UniProt

P10474 - GUNB_CALSA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Endoglucanase/exoglucanase B

Gene

celB

Organism
Caldicellulosiruptor saccharolyticus (Caldocellum saccharolyticum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This protein is made up of two domains: the N-terminal domain has exoglucanase activity while the C-terminal domain is an endoglucanase.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei177 – 1771Proton donorSequence Analysis
Active sitei285 – 2851NucleophilePROSITE-ProRule annotation
Active sitei792 – 7921By similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC
  2. cellulose 1,4-beta-cellobiosidase activity Source: UniProtKB-EC
  3. cellulose binding Source: InterPro

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiCBM3. Carbohydrate-Binding Module Family 3.
GH10. Glycoside Hydrolase Family 10.
GH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase/exoglucanase B
Including the following 2 domains:
Alternative name(s):
Cellobiohydrolase
Cellulase
Endo-1,4-beta-glucanase
Exoglucanase (EC:3.2.1.91)
Alternative name(s):
1,4-beta-cellobiohydrolase
Exocellobiohydrolase
Gene namesi
Name:celB
OrganismiCaldicellulosiruptor saccharolyticus (Caldocellum saccharolyticum)
Taxonomic identifieri44001 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisCaldicellulosiruptor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Add
BLAST
Chaini29 – 10391011Endoglucanase/exoglucanase BPRO_0000007983Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP10474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini418 – 571154CBM3PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi376 – 41641Pro/Thr-richAdd
BLAST
Compositional biasi574 – 61845Pro/Thr-richAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyl hydrolase 10 (cellulase F) family.Curated
In the C-terminal section; belongs to the glycosyl hydrolase 5 (cellulase A) family.Curated
Contains 1 CBM3 (carbohydrate binding type-3) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.60.40.710. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR008965. Carb-bd_dom.
IPR001956. CBD_3.
IPR001000. Glyco_hydro_10.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00942. CBM_3. 1 hit.
PF00150. Cellulase. 1 hit.
PF00331. Glyco_hydro_10. 1 hit.
[Graphical view]
PRINTSiPR00134. GLHYDRLASE10.
SMARTiSM01067. CBM_3. 1 hit.
SM00633. Glyco_10. 1 hit.
[Graphical view]
SUPFAMiSSF49384. SSF49384. 1 hit.
SSF51445. SSF51445. 2 hits.
PROSITEiPS51172. CBM3. 1 hit.
PS00591. GLYCOSYL_HYDROL_F10. 1 hit.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10474-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKRNLFRIVS RVVLIAFIAS ISLVGAMSYF PVETQAAPDW SIPSLCESYK
60 70 80 90 100
DDFMIGVAIP ARCLSNDTDK RMVLKHFNSI TAENEMKPES LLAGQTSTGL
110 120 130 140 150
SYRFSTADAF VDFASTNKIG IRGHTLVWHN QTPDWFFKDS NGQRLSKDAL
160 170 180 190 200
LARLKQYIYD VVGRYKGKVY AWDVVNEAID ENQPDSYRRS TWYEICGPEY
210 220 230 240 250
IEKAFIWAHE ADPNAKLFYN DYNTEISKKR DFIYNMVKNL KSKGIPIHGI
260 270 280 290 300
GMQCHINVNW PSVSEIENSI KLFSSIPGIE IHITELDMSL YNYGSSENYS
310 320 330 340 350
TPPQDLLQKQ SQKYKEIFTM LKKYKNVVKS VTFWGLKDDY SWLRSFYGKN
360 370 380 390 400
DWPLLFFEDY SAKPAYWAVI EASGVTTSSP TPTPTPTVTV TPTPTPTPTP
410 420 430 440 450
TVTATPTPTP TPVSTPATGG QIKVLYANKE TNSTTNTIRP WLKVVNSGSS
460 470 480 490 500
SIDLSRVTIR YWYTVDGERA QSAVSDWAQI GASNVTFKFV KLSSSVSGAD
510 520 530 540 550
YYLEIGFKSG AGQLQPGKDT GEIQIRFNKS DWSNYNQGND WSWLQSMTSY
560 570 580 590 600
GENEKVTAYI DGVLVWGQEP SGATPAPTMT VAPTATPTPT LSPTVTPTPA
610 620 630 640 650
PTQTAIPTPT LTPNPTPTSS IPDDTNDDWL YVSGNKIVDK DGRPVWLTGI
660 670 680 690 700
NWFGYNTGTN VFDGVWSCNL KDTLAEIANR GFNLLRVPIS AELILNWSQG
710 720 730 740 750
IYPKPNINYY VNPELEGKNS LEVFDIVVQT CKEVGLKIML DIHSIKTDAM
760 770 780 790 800
GHIYPVWYDE KFTPEDFYKA CEWITNRYKN DDTIIAFDLK NEPHGKPWQD
810 820 830 840 850
TTFAKWDNST DINNWKYAAE TCAKRILNIN PNLLIVIEGI EAYPKDDVTW
860 870 880 890 900
TSKSSSDYYS TWWGGNLRGV RKYPINLGKY QNKVVYSPHD YGPSVYQQPW
910 920 930 940 950
FYPGFTKESL LQDCWRPNWA YIMEENIAPL LIGEWGGHLD GADNEKWMKY
960 970 980 990 1000
LRDYIIENHI HHTFWCFNAN SGDTGGLVGY DFTTWDEKKY SFLKPALWQD
1010 1020 1030
SQGRFVGLDH KRPLGTNGKN INITTYYNNN EPEPVPASK
Length:1,039
Mass (Da):117,641
Last modified:July 1, 1989 - v1
Checksum:i0E0378171594DDAE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13602 Genomic DNA. Translation: CAA31936.1.
PIRiS02711.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13602 Genomic DNA. Translation: CAA31936.1 .
PIRi S02711.

3D structure databases

ProteinModelPortali P10474.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM3. Carbohydrate-Binding Module Family 3.
GH10. Glycoside Hydrolase Family 10.
GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.40.710. 1 hit.
3.20.20.80. 2 hits.
InterProi IPR008965. Carb-bd_dom.
IPR001956. CBD_3.
IPR001000. Glyco_hydro_10.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00942. CBM_3. 1 hit.
PF00150. Cellulase. 1 hit.
PF00331. Glyco_hydro_10. 1 hit.
[Graphical view ]
PRINTSi PR00134. GLHYDRLASE10.
SMARTi SM01067. CBM_3. 1 hit.
SM00633. Glyco_10. 1 hit.
[Graphical view ]
SUPFAMi SSF49384. SSF49384. 1 hit.
SSF51445. SSF51445. 2 hits.
PROSITEi PS51172. CBM3. 1 hit.
PS00591. GLYCOSYL_HYDROL_F10. 1 hit.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of a gene from Caldocellum saccharolyticum encoding for exocellulase and endocellulase activity."
    Saul D.J., Williams L.C., Love D.R., Chamley L.W., Bergquist P.L.
    Nucleic Acids Res. 17:439-439(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiGUNB_CALSA
AccessioniPrimary (citable) accession number: P10474
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 1, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3