P10474 (GUNB_CALSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase/exoglucanase B

Including the following 2 domains:

1. Endoglucanase
   EC=3.2.1.4
   Alternative name(s):
   Cellobiohydrolase
   Cellulase
   Endo-1,4-beta-glucanase
2. Exoglucanase
   EC=3.2.1.91
   Alternative name(s):
   1,4-beta-cellobiohydrolase
   Exocellobiohydrolase

Gene names

Name:

celB

Organism

Caldocellum saccharolyticum (Caldicellulosiruptor saccharolyticus)

Taxonomic identifier

44001 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacterales Family III. Incertae Sedis › Caldicellulosiruptor

Protein attributes

Sequence length	1039 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein is made up of two domains: the N-terminal domain has exoglucanase activity while the C-terminal domain is an endoglucanase.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.
Sequence similarities	In the N-terminal section; belongs to the glycosyl hydrolase 10 (cellulase F) family. In the C-terminal section; belongs to the glycosyl hydrolase 5 (cellulase A) family. Contains 1 CBM3 (carbohydrate binding type-3) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC cellulose 1,4-beta-cellobiosidase activity Inferred from electronic annotation. Source: EC cellulose binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28
	Chain	29 – 1039	1011	Endoglucanase/exoglucanase B		PRO_0000007983
Regions
	Domain	418 – 571	154	CBM3
	Compositional bias	376 – 416	41	Pro/Thr-rich
	Compositional bias	574 – 618	45	Pro/Thr-rich
Sites
	Active site	177	1	Proton donor Potential
	Active site	285	1	Nucleophile By similarity
	Active site	792	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P10474 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 0E0378171594DDAE	FASTA	1,039	117,641
	10 20 30 40 50 60 MKRNLFRIVS RVVLIAFIAS ISLVGAMSYF PVETQAAPDW SIPSLCESYK DDFMIGVAIP 70 80 90 100 110 120 ARCLSNDTDK RMVLKHFNSI TAENEMKPES LLAGQTSTGL SYRFSTADAF VDFASTNKIG 130 140 150 160 170 180 IRGHTLVWHN QTPDWFFKDS NGQRLSKDAL LARLKQYIYD VVGRYKGKVY AWDVVNEAID 190 200 210 220 230 240 ENQPDSYRRS TWYEICGPEY IEKAFIWAHE ADPNAKLFYN DYNTEISKKR DFIYNMVKNL 250 260 270 280 290 300 KSKGIPIHGI GMQCHINVNW PSVSEIENSI KLFSSIPGIE IHITELDMSL YNYGSSENYS 310 320 330 340 350 360 TPPQDLLQKQ SQKYKEIFTM LKKYKNVVKS VTFWGLKDDY SWLRSFYGKN DWPLLFFEDY 370 380 390 400 410 420 SAKPAYWAVI EASGVTTSSP TPTPTPTVTV TPTPTPTPTP TVTATPTPTP TPVSTPATGG 430 440 450 460 470 480 QIKVLYANKE TNSTTNTIRP WLKVVNSGSS SIDLSRVTIR YWYTVDGERA QSAVSDWAQI 490 500 510 520 530 540 GASNVTFKFV KLSSSVSGAD YYLEIGFKSG AGQLQPGKDT GEIQIRFNKS DWSNYNQGND 550 560 570 580 590 600 WSWLQSMTSY GENEKVTAYI DGVLVWGQEP SGATPAPTMT VAPTATPTPT LSPTVTPTPA 610 620 630 640 650 660 PTQTAIPTPT LTPNPTPTSS IPDDTNDDWL YVSGNKIVDK DGRPVWLTGI NWFGYNTGTN 670 680 690 700 710 720 VFDGVWSCNL KDTLAEIANR GFNLLRVPIS AELILNWSQG IYPKPNINYY VNPELEGKNS 730 740 750 760 770 780 LEVFDIVVQT CKEVGLKIML DIHSIKTDAM GHIYPVWYDE KFTPEDFYKA CEWITNRYKN 790 800 810 820 830 840 DDTIIAFDLK NEPHGKPWQD TTFAKWDNST DINNWKYAAE TCAKRILNIN PNLLIVIEGI 850 860 870 880 890 900 EAYPKDDVTW TSKSSSDYYS TWWGGNLRGV RKYPINLGKY QNKVVYSPHD YGPSVYQQPW 910 920 930 940 950 960 FYPGFTKESL LQDCWRPNWA YIMEENIAPL LIGEWGGHLD GADNEKWMKY LRDYIIENHI 970 980 990 1000 1010 1020 HHTFWCFNAN SGDTGGLVGY DFTTWDEKKY SFLKPALWQD SQGRFVGLDH KRPLGTNGKN 1030 INITTYYNNN EPEPVPASK

References

[1]	"Nucleotide sequence of a gene from Caldocellum saccharolyticum encoding for exocellulase and endocellulase activity." Saul D.J., Williams L.C., Love D.R., Chamley L.W., Bergquist P.L. Nucleic Acids Res. 17:439-439(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X13602 Genomic DNA. Translation: CAA31936.1.
PIR	S02711.
3D structure databases
ProteinModelPortal	P10474.
ModBase	Search...
Protein family/group databases
CAZy	CBM3. Carbohydrate-Binding Module Family 3. GH10. Glycoside Hydrolase Family 10. GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.60.40.710. 1 hit. 3.20.20.80. 2 hits.
InterPro	IPR008965. Carb-bd_dom. IPR001956. CBD_3. IPR001000. Glyco_hydro_10. IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00942. CBM_3. 1 hit. PF00150. Cellulase. 1 hit. PF00331. Glyco_hydro_10. 1 hit. [Graphical view]
PRINTS	PR00134. GLHYDRLASE10.
SMART	SM01067. CBM_3. 1 hit. SM00633. Glyco_10. 1 hit. [Graphical view]
SUPFAM	SSF49384. Cellul_bind. 1 hit. SSF51445. Glyco_hydro_cat. 2 hits.
PROSITE	PS51172. CBM3. 1 hit. PS00591. GLYCOSYL_HYDROL_F10. 1 hit. PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUNB_CALSA

Accession

Primary (citable) accession number: P10474

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program