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P10474

- GUNB_CALSA

UniProt

P10474 - GUNB_CALSA

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Protein

Endoglucanase/exoglucanase B

Gene
celB
Organism
Caldicellulosiruptor saccharolyticus (Caldocellum saccharolyticum)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

This protein is made up of two domains: the N-terminal domain has exoglucanase activity while the C-terminal domain is an endoglucanase.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei177 – 1771Proton donor Reviewed prediction
Active sitei285 – 2851Nucleophile By similarity
Active sitei792 – 7921 By similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC
  2. cellulose 1,4-beta-cellobiosidase activity Source: UniProtKB-EC
  3. cellulose binding Source: InterPro

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiCBM3. Carbohydrate-Binding Module Family 3.
GH10. Glycoside Hydrolase Family 10.
GH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase/exoglucanase B
Including the following 2 domains:
Alternative name(s):
Cellobiohydrolase
Cellulase
Endo-1,4-beta-glucanase
Exoglucanase (EC:3.2.1.91)
Alternative name(s):
1,4-beta-cellobiohydrolase
Exocellobiohydrolase
Gene namesi
Name:celB
OrganismiCaldicellulosiruptor saccharolyticus (Caldocellum saccharolyticum)
Taxonomic identifieri44001 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisCaldicellulosiruptor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Add
BLAST
Chaini29 – 10391011Endoglucanase/exoglucanase BPRO_0000007983Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP10474.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini418 – 571154CBM3Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi376 – 41641Pro/Thr-richAdd
BLAST
Compositional biasi574 – 61845Pro/Thr-richAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyl hydrolase 10 (cellulase F) family.
In the C-terminal section; belongs to the glycosyl hydrolase 5 (cellulase A) family.

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.60.40.710. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR008965. Carb-bd_dom.
IPR001956. CBD_3.
IPR001000. Glyco_hydro_10.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00942. CBM_3. 1 hit.
PF00150. Cellulase. 1 hit.
PF00331. Glyco_hydro_10. 1 hit.
[Graphical view]
PRINTSiPR00134. GLHYDRLASE10.
SMARTiSM01067. CBM_3. 1 hit.
SM00633. Glyco_10. 1 hit.
[Graphical view]
SUPFAMiSSF49384. SSF49384. 1 hit.
SSF51445. SSF51445. 2 hits.
PROSITEiPS51172. CBM3. 1 hit.
PS00591. GLYCOSYL_HYDROL_F10. 1 hit.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10474-1 [UniParc]FASTAAdd to Basket

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MKRNLFRIVS RVVLIAFIAS ISLVGAMSYF PVETQAAPDW SIPSLCESYK     50
DDFMIGVAIP ARCLSNDTDK RMVLKHFNSI TAENEMKPES LLAGQTSTGL 100
SYRFSTADAF VDFASTNKIG IRGHTLVWHN QTPDWFFKDS NGQRLSKDAL 150
LARLKQYIYD VVGRYKGKVY AWDVVNEAID ENQPDSYRRS TWYEICGPEY 200
IEKAFIWAHE ADPNAKLFYN DYNTEISKKR DFIYNMVKNL KSKGIPIHGI 250
GMQCHINVNW PSVSEIENSI KLFSSIPGIE IHITELDMSL YNYGSSENYS 300
TPPQDLLQKQ SQKYKEIFTM LKKYKNVVKS VTFWGLKDDY SWLRSFYGKN 350
DWPLLFFEDY SAKPAYWAVI EASGVTTSSP TPTPTPTVTV TPTPTPTPTP 400
TVTATPTPTP TPVSTPATGG QIKVLYANKE TNSTTNTIRP WLKVVNSGSS 450
SIDLSRVTIR YWYTVDGERA QSAVSDWAQI GASNVTFKFV KLSSSVSGAD 500
YYLEIGFKSG AGQLQPGKDT GEIQIRFNKS DWSNYNQGND WSWLQSMTSY 550
GENEKVTAYI DGVLVWGQEP SGATPAPTMT VAPTATPTPT LSPTVTPTPA 600
PTQTAIPTPT LTPNPTPTSS IPDDTNDDWL YVSGNKIVDK DGRPVWLTGI 650
NWFGYNTGTN VFDGVWSCNL KDTLAEIANR GFNLLRVPIS AELILNWSQG 700
IYPKPNINYY VNPELEGKNS LEVFDIVVQT CKEVGLKIML DIHSIKTDAM 750
GHIYPVWYDE KFTPEDFYKA CEWITNRYKN DDTIIAFDLK NEPHGKPWQD 800
TTFAKWDNST DINNWKYAAE TCAKRILNIN PNLLIVIEGI EAYPKDDVTW 850
TSKSSSDYYS TWWGGNLRGV RKYPINLGKY QNKVVYSPHD YGPSVYQQPW 900
FYPGFTKESL LQDCWRPNWA YIMEENIAPL LIGEWGGHLD GADNEKWMKY 950
LRDYIIENHI HHTFWCFNAN SGDTGGLVGY DFTTWDEKKY SFLKPALWQD 1000
SQGRFVGLDH KRPLGTNGKN INITTYYNNN EPEPVPASK 1039
Length:1,039
Mass (Da):117,641
Last modified:July 1, 1989 - v1
Checksum:i0E0378171594DDAE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X13602 Genomic DNA. Translation: CAA31936.1.
PIRiS02711.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X13602 Genomic DNA. Translation: CAA31936.1 .
PIRi S02711.

3D structure databases

ProteinModelPortali P10474.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM3. Carbohydrate-Binding Module Family 3.
GH10. Glycoside Hydrolase Family 10.
GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.40.710. 1 hit.
3.20.20.80. 2 hits.
InterProi IPR008965. Carb-bd_dom.
IPR001956. CBD_3.
IPR001000. Glyco_hydro_10.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00942. CBM_3. 1 hit.
PF00150. Cellulase. 1 hit.
PF00331. Glyco_hydro_10. 1 hit.
[Graphical view ]
PRINTSi PR00134. GLHYDRLASE10.
SMARTi SM01067. CBM_3. 1 hit.
SM00633. Glyco_10. 1 hit.
[Graphical view ]
SUPFAMi SSF49384. SSF49384. 1 hit.
SSF51445. SSF51445. 2 hits.
PROSITEi PS51172. CBM3. 1 hit.
PS00591. GLYCOSYL_HYDROL_F10. 1 hit.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of a gene from Caldocellum saccharolyticum encoding for exocellulase and endocellulase activity."
    Saul D.J., Williams L.C., Love D.R., Chamley L.W., Bergquist P.L.
    Nucleic Acids Res. 17:439-439(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiGUNB_CALSA
AccessioniPrimary (citable) accession number: P10474
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: December 11, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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