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Protein

Osteopontin

Gene

SPP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Biomineralization, Cell adhesion

Keywords - Ligandi

Sialic acid

Enzyme and pathway databases

BioCyciZFISH:ENSG00000118785-MONOMER.
ReactomeiR-HSA-1474228. Degradation of the extracellular matrix.
R-HSA-186797. Signaling by PDGF.
R-HSA-216083. Integrin cell surface interactions.
SIGNORiP10451.

Names & Taxonomyi

Protein namesi
Recommended name:
Osteopontin
Alternative name(s):
Bone sialoprotein 1
Nephropontin
Secreted phosphoprotein 1
Short name:
SPP-1
Urinary stone protein
Uropontin
Gene namesi
Name:SPP1
Synonyms:BNSP, OPN
ORF Names:PSEC0156
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:11255. SPP1.

Subcellular locationi

GO - Cellular componenti

  • cell projection Source: Ensembl
  • extracellular exosome Source: UniProtKB
  • extracellular region Source: Reactome
  • extracellular space Source: UniProtKB
  • perinuclear region of cytoplasm Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi6696.
MalaCardsiSPP1.
OpenTargetsiENSG00000118785.
Orphaneti93552. Pediatric systemic lupus erythematosus.
536. Systemic lupus erythematosus.
PharmGKBiPA36085.

Polymorphism and mutation databases

BioMutaiSPP1.
DMDMi129260.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000002032117 – 314OsteopontinAdd BLAST298

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24Phosphoserine1 Publication1
Modified residuei26Phosphoserine; by FAM20C2 Publications1
Modified residuei27Phosphoserine; by FAM20C2 Publications1
Modified residuei62Phosphoserine; by FAM20C2 Publications1
Modified residuei63Phosphoserine; by FAM20C2 Publications1
Modified residuei66Phosphothreonine1 Publication1
Modified residuei76Phosphoserine1 Publication1
Modified residuei78PhosphoserineBy similarity1
Glycosylationi79N-linked (GlcNAc...)1 Publication1
Modified residuei81PhosphoserineCombined sources1 Publication1
Modified residuei99Phosphoserine1 Publication1
Modified residuei102Phosphoserine1 Publication1
Modified residuei105Phosphoserine1 Publication1
Glycosylationi106N-linked (GlcNAc...)1 Publication1
Modified residuei108Phosphoserine1 Publication1
Modified residuei117Phosphoserine1 Publication1
Modified residuei120Phosphoserine1 Publication1
Modified residuei123Phosphoserine1 Publication1
Modified residuei126Phosphoserine1 Publication1
Modified residuei129Phosphoserine1 Publication1
Glycosylationi134O-linked (GalNAc...)1 Publication1
Glycosylationi138O-linked (GalNAc...)1 Publication1
Glycosylationi143O-linked (GalNAc...)1 Publication1
Glycosylationi147O-linked (GalNAc...)1 Publication1
Glycosylationi152O-linked (GalNAc...)1 Publication1
Modified residuei185Phosphothreonine1 Publication1
Modified residuei190Phosphothreonine; by FAM20C1 Publication1
Modified residuei191Phosphoserine; by FAM20C2 Publications1
Modified residuei195Phosphoserine; by FAM20C2 Publications1
Modified residuei215Phosphoserine; by FAM20C2 Publications1
Modified residuei219Phosphoserine; by FAM20CCombined sources2 Publications1
Modified residuei224Phosphoserine; by FAM20CCombined sources2 Publications1
Modified residuei225Phosphotyrosine1 Publication1
Modified residuei228Phosphoserine; by FAM20C2 Publications1
Modified residuei234Phosphoserine; by FAM20CCombined sources2 Publications1
Modified residuei237Phosphothreonine; by FAM20C1 Publication1
Modified residuei239Phosphoserine; by FAM20C1 Publication1
Modified residuei243Phosphoserine; by FAM20C1 Publication1
Modified residuei254Phosphoserine; by FAM20CCombined sources2 Publications1
Modified residuei258Phosphoserine; by FAM20C1 Publication1
Modified residuei263Phosphoserine; by FAM20C2 Publications1
Modified residuei267Phosphoserine; by FAM20C2 Publications1
Modified residuei270Phosphoserine; by FAM20C1 Publication1
Modified residuei275Phosphoserine; by FAM20C2 Publications1
Modified residuei280Phosphoserine; by FAM20C2 Publications1
Modified residuei291Phosphoserine; by FAM20C1 Publication1
Modified residuei303Phosphoserine; by FAM20C2 Publications1
Modified residuei308Phosphoserine; by FAM20C2 Publications1
Modified residuei310Phosphoserine; by FAM20C2 Publications1
Modified residuei311Phosphoserine; by FAM20C1 Publication1

Post-translational modificationi

Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.3 Publications
N- and O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62.2 Publications

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP10451.
PeptideAtlasiP10451.
PRIDEiP10451.

PTM databases

iPTMnetiP10451.
PhosphoSitePlusiP10451.
UniCarbKBiP10451.

Miscellaneous databases

PMAP-CutDBP10451.

Expressioni

Tissue specificityi

Bone. Found in plasma.

Gene expression databases

BgeeiENSG00000118785.
CleanExiHS_SPP1.
ExpressionAtlasiP10451. baseline and differential.
GenevisibleiP10451. HS.

Organism-specific databases

HPAiCAB002212.
HPA027541.

Interactioni

Subunit structurei

Ligand for integrin alpha-V/beta-3.

Binary interactionsi

WithEntry#Exp.IntActNotes
CD44P160704EBI-723648,EBI-490245
MEOX2A4D1273EBI-723648,EBI-10172134
SGTAO437655EBI-723648,EBI-347996

Protein-protein interaction databases

BioGridi112574. 79 interactors.
DIPiDIP-49933N.
IntActiP10451. 13 interactors.
MINTiMINT-1380527.
STRINGi9606.ENSP00000378517.

Structurei

Secondary structure

1314
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi44 – 47Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CXDX-ray2.80P40-51[»]
3DSFX-ray2.80P40-51[»]
DisProtiDP00214.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10451.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi159 – 161Cell attachment site3

Sequence similaritiesi

Belongs to the osteopontin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IVP1. Eukaryota.
ENOG41116B5. LUCA.
GeneTreeiENSGT00390000002509.
HOGENOMiHOG000059656.
HOVERGENiHBG001731.
InParanoidiP10451.
KOiK06250.
OMAiVICFCLL.
OrthoDBiEOG091G0KVC.
PhylomeDBiP10451.
TreeFamiTF350201.

Family and domain databases

InterProiIPR002038. Osteopontin.
IPR019841. Osteopontin_CS.
[Graphical view]
PANTHERiPTHR10607. PTHR10607. 1 hit.
PfamiPF00865. Osteopontin. 1 hit.
[Graphical view]
PRINTSiPR00216. OSTEOPONTIN.
SMARTiSM00017. OSTEO. 1 hit.
[Graphical view]
PROSITEiPS00884. OSTEOPONTIN. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P10451-1) [UniParc]FASTAAdd to basket
Also known as: OPN-a, OP1B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRIAVICFCL LGITCAIPVK QADSGSSEEK QLYNKYPDAV ATWLNPDPSQ
60 70 80 90 100
KQNLLAPQNA VSSEETNDFK QETLPSKSNE SHDHMDDMDD EDDDDHVDSQ
110 120 130 140 150
DSIDSNDSDD VDDTDDSHQS DESHHSDESD ELVTDFPTDL PATEVFTPVV
160 170 180 190 200
PTVDTYDGRG DSVVYGLRSK SKKFRRPDIQ YPDATDEDIT SHMESEELNG
210 220 230 240 250
AYKAIPVAQD LNAPSDWDSR GKDSYETSQL DDQSAETHSH KQSRLYKRKA
260 270 280 290 300
NDESNEHSDV IDSQELSKVS REFHSHEFHS HEDMLVVDPK SKEEDKHLKF
310
RISHELDSAS SEVN
Length:314
Mass (Da):35,423
Last modified:July 1, 1989 - v1
Checksum:i4996429EC4752B86
GO
Isoform B (identifier: P10451-2) [UniParc]FASTAAdd to basket
Also known as: OPN-b, OP1A

The sequence of this isoform differs from the canonical sequence as follows:
     58-71: Missing.

Show »
Length:300
Mass (Da):33,844
Checksum:iE844D1466BD20E64
GO
Isoform C (identifier: P10451-3) [UniParc]FASTAAdd to basket
Also known as: OPN-c

The sequence of this isoform differs from the canonical sequence as follows:
     31-57: Missing.

Show »
Length:287
Mass (Da):32,355
Checksum:iAF604D4E223BA02F
GO
Isoform D (identifier: P10451-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     95-116: Missing.

Show »
Length:292
Mass (Da):33,017
Checksum:iE93E418982C3AD60
GO
Isoform 5 (identifier: P10451-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     59-72: Missing.

Note: No experimental confirmation available.
Show »
Length:300
Mass (Da):33,843
Checksum:iB844D7EBDD096CBF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti188D → H in BAA05949 (PubMed:7837791).Curated1
Sequence conflicti188D → H in BAA05950 (PubMed:7837791).Curated1
Sequence conflicti188D → H in BAA05951 (PubMed:7837791).Curated1
Sequence conflicti237T → A in BAA05949 (PubMed:7837791).Curated1
Sequence conflicti237T → A in BAA05950 (PubMed:7837791).Curated1
Sequence conflicti237T → A in BAA05951 (PubMed:7837791).Curated1
Sequence conflicti275 – 278SHEF → GNSL (PubMed:1974876).Curated4

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050432224S → N.Corresponds to variant rs7435825dbSNPEnsembl.1
Natural variantiVAR_014717301R → H.Corresponds to variant rs4660dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00377731 – 57Missing in isoform C. 4 PublicationsAdd BLAST27
Alternative sequenceiVSP_00377858 – 71Missing in isoform B. 4 PublicationsAdd BLAST14
Alternative sequenceiVSP_04369559 – 72Missing in isoform 5. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_01163995 – 116Missing in isoform D. 1 PublicationAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13694 mRNA. Translation: CAA31984.1.
J04765 mRNA. Translation: AAA59974.1.
M83248 mRNA. Translation: AAA17675.1.
U20758 Genomic DNA. Translation: AAA86886.1.
D14813 Genomic DNA. Translation: BAA03554.1.
D28759 mRNA. Translation: BAA05949.1.
D28760 mRNA. Translation: BAA05950.1.
D28761 mRNA. Translation: BAA05951.1.
AF052124 mRNA. Translation: AAC28619.1.
AK290104 mRNA. Translation: BAF82793.1.
JF412667 mRNA. Translation: AEA49031.1.
AK075463 mRNA. Translation: BAC11635.1.
AK290090 mRNA. Translation: BAF82779.1.
AK296035 mRNA. Translation: BAG58801.1.
AK315461 mRNA. Translation: BAG37848.1.
DQ839491 mRNA. Translation: ABI63352.1.
DQ846871 mRNA. Translation: ABI63358.1.
AC131944 Genomic DNA. Translation: AAY41035.1.
CH471057 Genomic DNA. Translation: EAX06004.1.
CH471057 Genomic DNA. Translation: EAX06005.1.
CH471057 Genomic DNA. Translation: EAX06006.1.
BC007016 mRNA. Translation: AAH07016.1.
BC017387 mRNA. Translation: AAH17387.1.
BC022844 mRNA. Translation: AAH22844.1.
BC093033 mRNA. Translation: AAH93033.1.
CCDSiCCDS34027.1. [P10451-3]
CCDS3626.1. [P10451-5]
CCDS43250.1. [P10451-1]
PIRiS50028. S09575.
RefSeqiNP_000573.1. NM_000582.2. [P10451-5]
NP_001035147.1. NM_001040058.1. [P10451-1]
NP_001035149.1. NM_001040060.1. [P10451-3]
NP_001238758.1. NM_001251829.1.
NP_001238759.1. NM_001251830.1.
UniGeneiHs.313.

Genome annotation databases

EnsembliENST00000237623; ENSP00000237623; ENSG00000118785. [P10451-5]
ENST00000360804; ENSP00000354042; ENSG00000118785. [P10451-3]
ENST00000395080; ENSP00000378517; ENSG00000118785. [P10451-1]
ENST00000614857; ENSP00000477824; ENSG00000118785. [P10451-4]
GeneIDi6696.
KEGGihsa:6696.
UCSCiuc003hra.4. human. [P10451-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology
Wikipedia

Osteopontin entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13694 mRNA. Translation: CAA31984.1.
J04765 mRNA. Translation: AAA59974.1.
M83248 mRNA. Translation: AAA17675.1.
U20758 Genomic DNA. Translation: AAA86886.1.
D14813 Genomic DNA. Translation: BAA03554.1.
D28759 mRNA. Translation: BAA05949.1.
D28760 mRNA. Translation: BAA05950.1.
D28761 mRNA. Translation: BAA05951.1.
AF052124 mRNA. Translation: AAC28619.1.
AK290104 mRNA. Translation: BAF82793.1.
JF412667 mRNA. Translation: AEA49031.1.
AK075463 mRNA. Translation: BAC11635.1.
AK290090 mRNA. Translation: BAF82779.1.
AK296035 mRNA. Translation: BAG58801.1.
AK315461 mRNA. Translation: BAG37848.1.
DQ839491 mRNA. Translation: ABI63352.1.
DQ846871 mRNA. Translation: ABI63358.1.
AC131944 Genomic DNA. Translation: AAY41035.1.
CH471057 Genomic DNA. Translation: EAX06004.1.
CH471057 Genomic DNA. Translation: EAX06005.1.
CH471057 Genomic DNA. Translation: EAX06006.1.
BC007016 mRNA. Translation: AAH07016.1.
BC017387 mRNA. Translation: AAH17387.1.
BC022844 mRNA. Translation: AAH22844.1.
BC093033 mRNA. Translation: AAH93033.1.
CCDSiCCDS34027.1. [P10451-3]
CCDS3626.1. [P10451-5]
CCDS43250.1. [P10451-1]
PIRiS50028. S09575.
RefSeqiNP_000573.1. NM_000582.2. [P10451-5]
NP_001035147.1. NM_001040058.1. [P10451-1]
NP_001035149.1. NM_001040060.1. [P10451-3]
NP_001238758.1. NM_001251829.1.
NP_001238759.1. NM_001251830.1.
UniGeneiHs.313.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CXDX-ray2.80P40-51[»]
3DSFX-ray2.80P40-51[»]
DisProtiDP00214.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112574. 79 interactors.
DIPiDIP-49933N.
IntActiP10451. 13 interactors.
MINTiMINT-1380527.
STRINGi9606.ENSP00000378517.

PTM databases

iPTMnetiP10451.
PhosphoSitePlusiP10451.
UniCarbKBiP10451.

Polymorphism and mutation databases

BioMutaiSPP1.
DMDMi129260.

Proteomic databases

PaxDbiP10451.
PeptideAtlasiP10451.
PRIDEiP10451.

Protocols and materials databases

DNASUi6696.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000237623; ENSP00000237623; ENSG00000118785. [P10451-5]
ENST00000360804; ENSP00000354042; ENSG00000118785. [P10451-3]
ENST00000395080; ENSP00000378517; ENSG00000118785. [P10451-1]
ENST00000614857; ENSP00000477824; ENSG00000118785. [P10451-4]
GeneIDi6696.
KEGGihsa:6696.
UCSCiuc003hra.4. human. [P10451-1]

Organism-specific databases

CTDi6696.
DisGeNETi6696.
GeneCardsiSPP1.
H-InvDBHIX0004361.
HGNCiHGNC:11255. SPP1.
HPAiCAB002212.
HPA027541.
MalaCardsiSPP1.
MIMi166490. gene.
neXtProtiNX_P10451.
OpenTargetsiENSG00000118785.
Orphaneti93552. Pediatric systemic lupus erythematosus.
536. Systemic lupus erythematosus.
PharmGKBiPA36085.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IVP1. Eukaryota.
ENOG41116B5. LUCA.
GeneTreeiENSGT00390000002509.
HOGENOMiHOG000059656.
HOVERGENiHBG001731.
InParanoidiP10451.
KOiK06250.
OMAiVICFCLL.
OrthoDBiEOG091G0KVC.
PhylomeDBiP10451.
TreeFamiTF350201.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000118785-MONOMER.
ReactomeiR-HSA-1474228. Degradation of the extracellular matrix.
R-HSA-186797. Signaling by PDGF.
R-HSA-216083. Integrin cell surface interactions.
SIGNORiP10451.

Miscellaneous databases

ChiTaRSiSPP1. human.
EvolutionaryTraceiP10451.
GeneWikiiOsteopontin.
GenomeRNAii6696.
PMAP-CutDBP10451.
PROiP10451.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000118785.
CleanExiHS_SPP1.
ExpressionAtlasiP10451. baseline and differential.
GenevisibleiP10451. HS.

Family and domain databases

InterProiIPR002038. Osteopontin.
IPR019841. Osteopontin_CS.
[Graphical view]
PANTHERiPTHR10607. PTHR10607. 1 hit.
PfamiPF00865. Osteopontin. 1 hit.
[Graphical view]
PRINTSiPR00216. OSTEOPONTIN.
SMARTiSM00017. OSTEO. 1 hit.
[Graphical view]
PROSITEiPS00884. OSTEOPONTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOSTP_HUMAN
AccessioniPrimary (citable) accession number: P10451
Secondary accession number(s): B2RDA1
, Q15681, Q15682, Q15683, Q4W597, Q567T5, Q8NBK2, Q96IZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 30, 2016
This is version 191 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.