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Protein

DNA polymerase III subunit alpha

Gene

dnaE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  • DNA binding Source: InterPro
  • DNA-directed DNA polymerase activity Source: EcoliWiki

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

BioCyciEcoCyc:EG10238-MONOMER.
ECOL316407:JW0179-MONOMER.
MetaCyc:EG10238-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III subunit alpha (EC:2.7.7.7)
Gene namesi
Name:dnaE
Synonyms:polC
Ordered Locus Names:b0184, JW0179
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10238. dnaE.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc
  • DNA polymerase III, core complex Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4621.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001033211 – 1160DNA polymerase III subunit alphaAdd BLAST1160

Proteomic databases

PaxDbiP10443.
PRIDEiP10443.

Interactioni

Subunit structurei

The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta', psi and chi, and copies of either or both of two different DnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta', psi,chi-beta[4].

Binary interactionsi

WithEntry#Exp.IntActNotes
dnaNP0A98817EBI-549111,EBI-542385
dnaQP0300720EBI-549111,EBI-549131
dnaXP0671010EBI-549111,EBI-549140
holAP286304EBI-549111,EBI-549153

Protein-protein interaction databases

BioGridi4262228. 207 interactors.
DIPiDIP-9458N.
IntActiP10443. 69 interactors.
MINTiMINT-1224103.
STRINGi511145.b0184.

Chemistry databases

BindingDBiP10443.

Structurei

Secondary structure

11160
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni14 – 17Combined sources4
Beta strandi18 – 20Combined sources3
Helixi25 – 33Combined sources9
Beta strandi37 – 44Combined sources8
Helixi50 – 59Combined sources10
Beta strandi63 – 73Combined sources11
Helixi75 – 77Combined sources3
Beta strandi82 – 88Combined sources7
Helixi91 – 106Combined sources16
Helixi120 – 124Combined sources5
Beta strandi128 – 131Combined sources4
Helixi134 – 136Combined sources3
Helixi138 – 145Combined sources8
Helixi148 – 161Combined sources14
Beta strandi166 – 170Combined sources5
Helixi178 – 192Combined sources15
Beta strandi196 – 198Combined sources3
Beta strandi204 – 206Combined sources3
Helixi207 – 209Combined sources3
Helixi210 – 221Combined sources12
Helixi243 – 249Combined sources7
Turni250 – 252Combined sources3
Helixi254 – 266Combined sources13
Beta strandi284 – 286Combined sources3
Helixi288 – 307Combined sources20
Helixi311 – 317Combined sources7
Helixi319 – 334Combined sources16
Helixi338 – 353Combined sources16
Helixi365 – 367Combined sources3
Helixi369 – 373Combined sources5
Turni381 – 385Combined sources5
Helixi388 – 390Combined sources3
Beta strandi405 – 407Combined sources3
Helixi408 – 410Combined sources3
Helixi411 – 422Combined sources12
Helixi424 – 426Combined sources3
Beta strandi427 – 430Combined sources4
Beta strandi433 – 435Combined sources3
Helixi438 – 448Combined sources11
Helixi453 – 460Combined sources8
Helixi471 – 477Combined sources7
Helixi480 – 487Combined sources8
Helixi489 – 501Combined sources13
Beta strandi505 – 508Combined sources4
Beta strandi514 – 517Combined sources4
Helixi522 – 524Combined sources3
Beta strandi538 – 541Combined sources4
Helixi543 – 548Combined sources6
Beta strandi552 – 556Combined sources5
Helixi561 – 574Combined sources14
Turni575 – 580Combined sources6
Helixi586 – 588Combined sources3
Helixi594 – 601Combined sources8
Beta strandi608 – 610Combined sources3
Helixi614 – 623Combined sources10
Helixi628 – 637Combined sources10
Helixi640 – 643Combined sources4
Helixi647 – 655Combined sources9
Beta strandi657 – 659Combined sources3
Beta strandi666 – 669Combined sources4
Helixi671 – 673Combined sources3
Helixi674 – 677Combined sources4
Helixi678 – 680Combined sources3
Helixi687 – 698Combined sources12
Helixi702 – 714Combined sources13
Helixi717 – 733Combined sources17
Helixi738 – 752Combined sources15
Helixi758 – 777Combined sources20
Helixi779 – 789Combined sources11
Turni790 – 792Combined sources3
Helixi794 – 806Combined sources13
Turni816 – 818Combined sources3
Beta strandi820 – 822Combined sources3
Helixi835 – 837Combined sources3
Helixi843 – 854Combined sources12
Beta strandi855 – 857Combined sources3
Helixi862 – 865Combined sources4
Beta strandi871 – 874Combined sources4
Helixi876 – 884Combined sources9
Turni885 – 891Combined sources7
Helixi895 – 899Combined sources5
Beta strandi902 – 905Combined sources4
Helixi906 – 909Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HNHX-ray2.30A1-910[»]
2HQAX-ray2.60A1-917[»]
4GX8X-ray1.70A/B/C/D1-270[»]
4GX9X-ray2.15A/B/C/D1-270[»]
4JOMX-ray2.90A1-917[»]
5FKUelectron microscopy8.34A1-1160[»]
5FKVelectron microscopy8.00A1-1160[»]
5FKWelectron microscopy7.30A1-1160[»]
ProteinModelPortaliP10443.
SMRiP10443.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10443.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C0B. Bacteria.
COG0587. LUCA.
HOGENOMiHOG000021784.
InParanoidiP10443.
KOiK02337.
OMAiMKFPGYM.
PhylomeDBiP10443.

Family and domain databases

InterProiIPR011708. DNA_pol3_alpha.
IPR029460. DNAPol_HHH.
IPR004365. NA-bd_OB_tRNA.
IPR004013. PHP_dom.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
IPR004805. PolC_alpha.
[Graphical view]
PfamiPF07733. DNA_pol3_alpha. 1 hit.
PF14579. HHH_6. 1 hit.
PF02811. PHP. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
SMARTiSM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMiSSF89550. SSF89550. 1 hit.
TIGRFAMsiTIGR00594. polc. 1 hit.

Sequencei

Sequence statusi: Complete.

P10443-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEPRFVHLR VHSDYSMIDG LAKTAPLVKK AAALGMPALA ITDFTNLCGL
60 70 80 90 100
VKFYGAGHGA GIKPIVGADF NVQCDLLGDE LTHLTVLAAN NTGYQNLTLL
110 120 130 140 150
ISKAYQRGYG AAGPIIDRDW LIELNEGLIL LSGGRMGDVG RSLLRGNSAL
160 170 180 190 200
VDECVAFYEE HFPDRYFLEL IRTGRPDEES YLHAAVELAE ARGLPVVATN
210 220 230 240 250
DVRFIDSSDF DAHEIRVAIH DGFTLDDPKR PRNYSPQQYM RSEEEMCELF
260 270 280 290 300
ADIPEALANT VEIAKRCNVT VRLGEYFLPQ FPTGDMSTED YLVKRAKEGL
310 320 330 340 350
EERLAFLFPD EEERLKRRPE YDERLETELQ VINQMGFPGY FLIVMEFIQW
360 370 380 390 400
SKDNGVPVGP GRGSGAGSLV AYALKITDLD PLEFDLLFER FLNPERVSMP
410 420 430 440 450
DFDVDFCMEK RDQVIEHVAD MYGRDAVSQI ITFGTMAAKA VIRDVGRVLG
460 470 480 490 500
HPYGFVDRIS KLIPPDPGMT LAKAFEAEPQ LPEIYEADEE VKALIDMARK
510 520 530 540 550
LEGVTRNAGK HAGGVVIAPT KITDFAPLYC DEEGKHPVTQ FDKSDVEYAG
560 570 580 590 600
LVKFDFLGLR TLTIINWALE MINKRRAKNG EPPLDIAAIP LDDKKSFDML
610 620 630 640 650
QRSETTAVFQ LESRGMKDLI KRLQPDCFED MIALVALFRP GPLQSGMVDN
660 670 680 690 700
FIDRKHGREE ISYPDVQWQH ESLKPVLEPT YGIILYQEQV MQIAQVLSGY
710 720 730 740 750
TLGGADMLRR AMGKKKPEEM AKQRSVFAEG AEKNGINAEL AMKIFDLVEK
760 770 780 790 800
FAGYGFNKSH SAAYALVSYQ TLWLKAHYPA EFMAAVMTAD MDNTEKVVGL
810 820 830 840 850
VDECWRMGLK ILPPDINSGL YHFHVNDDGE IVYGIGAIKG VGEGPIEAII
860 870 880 890 900
EARNKGGYFR ELFDLCARTD TKKLNRRVLE KLIMSGAFDR LGPHRAALMN
910 920 930 940 950
SLGDALKAAD QHAKAEAIGQ ADMFGVLAEE PEQIEQSYAS CQPWPEQVVL
960 970 980 990 1000
DGERETLGLY LTGHPINQYL KEIERYVGGV RLKDMHPTER GKVITAAGLV
1010 1020 1030 1040 1050
VAARVMVTKR GNRIGICTLD DRSGRLEVML FTDALDKYQQ LLEKDRILIV
1060 1070 1080 1090 1100
SGQVSFDDFS GGLKMTAREV MDIDEAREKY ARGLAISLTD RQIDDQLLNR
1110 1120 1130 1140 1150
LRQSLEPHRS GTIPVHLYYQ RADARARLRF GATWRVSPSD RLLNDLRGLI
1160
GSEQVELEFD
Length:1,160
Mass (Da):129,905
Last modified:July 1, 1989 - v1
Checksum:i1A4F75F373841716
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19334 Genomic DNA. Translation: AAC36920.1.
U70214 Genomic DNA. Translation: AAB08613.1.
U00096 Genomic DNA. Translation: AAC73295.1.
AP009048 Genomic DNA. Translation: BAA77859.1.
S52931 mRNA. Translation: AAB24889.1.
M96394 Genomic DNA. Translation: AAA70369.1. Sequence problems.
D49445 Genomic DNA. Translation: BAA08424.1.
PIRiC28390. DJEC3A.
RefSeqiNP_414726.1. NC_000913.3.
WP_001294757.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73295; AAC73295; b0184.
BAA77859; BAA77859; BAA77859.
GeneIDi944877.
KEGGiecj:JW0179.
eco:b0184.
PATRICi32115479. VBIEscCol129921_0191.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19334 Genomic DNA. Translation: AAC36920.1.
U70214 Genomic DNA. Translation: AAB08613.1.
U00096 Genomic DNA. Translation: AAC73295.1.
AP009048 Genomic DNA. Translation: BAA77859.1.
S52931 mRNA. Translation: AAB24889.1.
M96394 Genomic DNA. Translation: AAA70369.1. Sequence problems.
D49445 Genomic DNA. Translation: BAA08424.1.
PIRiC28390. DJEC3A.
RefSeqiNP_414726.1. NC_000913.3.
WP_001294757.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HNHX-ray2.30A1-910[»]
2HQAX-ray2.60A1-917[»]
4GX8X-ray1.70A/B/C/D1-270[»]
4GX9X-ray2.15A/B/C/D1-270[»]
4JOMX-ray2.90A1-917[»]
5FKUelectron microscopy8.34A1-1160[»]
5FKVelectron microscopy8.00A1-1160[»]
5FKWelectron microscopy7.30A1-1160[»]
ProteinModelPortaliP10443.
SMRiP10443.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262228. 207 interactors.
DIPiDIP-9458N.
IntActiP10443. 69 interactors.
MINTiMINT-1224103.
STRINGi511145.b0184.

Chemistry databases

BindingDBiP10443.
ChEMBLiCHEMBL4621.

Proteomic databases

PaxDbiP10443.
PRIDEiP10443.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73295; AAC73295; b0184.
BAA77859; BAA77859; BAA77859.
GeneIDi944877.
KEGGiecj:JW0179.
eco:b0184.
PATRICi32115479. VBIEscCol129921_0191.

Organism-specific databases

EchoBASEiEB0234.
EcoGeneiEG10238. dnaE.

Phylogenomic databases

eggNOGiENOG4105C0B. Bacteria.
COG0587. LUCA.
HOGENOMiHOG000021784.
InParanoidiP10443.
KOiK02337.
OMAiMKFPGYM.
PhylomeDBiP10443.

Enzyme and pathway databases

BioCyciEcoCyc:EG10238-MONOMER.
ECOL316407:JW0179-MONOMER.
MetaCyc:EG10238-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP10443.
PROiP10443.

Family and domain databases

InterProiIPR011708. DNA_pol3_alpha.
IPR029460. DNAPol_HHH.
IPR004365. NA-bd_OB_tRNA.
IPR004013. PHP_dom.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
IPR004805. PolC_alpha.
[Graphical view]
PfamiPF07733. DNA_pol3_alpha. 1 hit.
PF14579. HHH_6. 1 hit.
PF02811. PHP. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
SMARTiSM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMiSSF89550. SSF89550. 1 hit.
TIGRFAMsiTIGR00594. polc. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDPO3A_ECOLI
AccessioniPrimary (citable) accession number: P10443
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 165 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.