lpxB

Functionⁱ

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.

Catalytic activityⁱ

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.1 Publication

Pathwayⁱ: lipid IV(A) biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:

This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functionⁱ

lipid-A-disaccharide synthase activity
Source: EcoliWiki
Inferred from direct assayⁱ
- 6370995
phospholipid binding
Source: EcoliWiki
Inferred from direct assayⁱ
- 19883124

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

lipid A biosynthetic process
Source: EcoliWiki
Inferred from mutant phenotypeⁱ
- 6370995

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Glycosyltransferase, Transferase
Biological process	Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCycⁱ	EcoCyc:LIPIDADISACCHARIDESYNTH-MONOMER MetaCyc:LIPIDADISACCHARIDESYNTH-MONOMER
UniPathwayⁱ	UPA00359; UER00481

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	GT19 Glycosyltransferase Family 19

CAZyⁱ

GT19 Glycosyltransferase Family 19

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Lipid-A-disaccharide synthase (EC:2.4.1.1821 Publication)
Gene namesⁱ	Name:lpxB Synonyms:pgsB Ordered Locus Names:b0182, JW0177
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10546 lpxB

EcoGeneⁱ

EG10546 lpxB

Subcellular locationⁱ

GO - Cellular componentⁱ

cytoplasm
Source: EcoCyc
Inferred from direct assayⁱ
- 6370995
cytosol
Source: EcoCyc
Inferred from direct assayⁱ
- 6370995
extrinsic component of plasma membrane
Source: EcoliWiki
Inferred from direct assayⁱ
- 19883124

View the complete GO annotation on QuickGO ...

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000190164	1 – 382	Lipid-A-disaccharide synthaseAdd BLAST		382

Proteomic databases

EPDⁱ	P10441
PaxDbⁱ	P10441
PRIDEⁱ	P10441

Interactionⁱ

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
tufA	P0CE47	3	EBI-553692,EBI-301077

With

Entry

#Exp.

IntAct

Notes

tufA

P0CE47

3

EBI-553692,EBI-301077

Protein-protein interaction databases

BioGridⁱ	4260870581 interactors.
Database of interacting proteins More...DIPⁱ	DIP-10122N
IntActⁱ	P10441 26 interactors.
STRINGⁱ	316385.ECDH10B_0162

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P10441
SMRⁱ	P10441
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the LpxB family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105D0V Bacteria COG0763 LUCA
HOGENOMⁱ	HOG000018003
InParanoidⁱ	P10441
KEGG Orthology (KO) More...KOⁱ	K00748
OMAⁱ	PTVWAWR
PhylomeDBⁱ	P10441

Family and domain databases

HAMAPⁱ	MF_00392 LpxB, 1 hit
InterProⁱ	View protein in InterPro IPR003835 Glyco_trans_19
PANTHERⁱ	PTHR30372 PTHR30372, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02684 LpxB, 1 hit
TIGRFAMsⁱ	TIGR00215 lpxB, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P10441-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTEQRPLTIA LVAGETSGDI LGAGLIRALK EHVPNARFVG VAGPRMQAEG 
        60         70         80         90        100
CEAWYEMEEL AVMGIVEVLG RLRRLLHIRA DLTKRFGELK PDVFVGIDAP 
       110        120        130        140        150
DFNITLEGNL KKQGIKTIHY VSPSVWAWRQ KRVFKIGRAT DLVLAFLPFE 
       160        170        180        190        200
KAFYDKYNVP CRFIGHTMAD AMPLDPDKNA ARDVLGIPHD AHCLALLPGS 
       210        220        230        240        250
RGAEVEMLSA DFLKTAQLLR QTYPDLEIVV PLVNAKRREQ FERIKAEVAP 
       260        270        280        290        300
DLSVHLLDGM GREAMVASDA ALLASGTAAL ECMLAKCPMV VGYRMKPFTF 
       310        320        330        340        350
WLAKRLVKTD YVSLPNLLAG RELVKELLQE ECEPQKLAAA LLPLLANGKT 
       360        370        380 
SHAMHDTFRE LHQQIRCNAD EQAAQAVLEL AQ

Length:382

Mass (Da):42,382

Last modified:July 19, 2003 - v2

Checksum:ⁱ12C76B6ECA438B47

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	149	F → V (PubMed:2824445).Curated		1
Sequence conflictⁱ	149	F → V (Ref. 2) Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M19334 Genomic DNA Translation: AAC36919.1 U70214 Genomic DNA Translation: AAB08611.1 U00096 Genomic DNA Translation: AAC73293.1 AP009048 Genomic DNA Translation: BAA77857.2
PIRⁱ	F64742 SYECLA
NCBI Reference Sequences More...RefSeqⁱ	NP_414724.1, NC_000913.3 WP_000139654.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC73293; AAC73293; b0182 BAA77857; BAA77857; BAA77857
GeneIDⁱ	944838
KEGGⁱ	ecj:JW0177 eco:b0182
PATRICⁱ	fig\|1411691.4.peg.2097

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P10441	A7ZHS2 C4ZRS4 B1XD51 A7ZWC8 Q3Z5H6 Q0P6M4 A0A0H7K682 V2QJN2 A0A0E0TUR3 A0A0E1SRJ9 +23	Escherichia coli O139:H28 (strain E24377A / ETEC) Escherichia coli (strain K12 / MC4100 / BW2952) Escherichia coli (strain K12 / DH10B) Escherichia coli O9:H4 (strain HS) Shigella sonnei (strain Ss046) Escherichia coli Shigella sonnei Escherichia coli HVH 50 (4-2593475) Escherichia coli UMNK88 Escherichia coli 53638 And more	382	UniRef100_A7ZHS2	Cluster: Lipid-A-disaccharide synthase	34

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P10441	B5Y1I9 G5QUZ6 UPI0009ADD023 UPI0005B6437E A6T4Y4 W1B729 A0A0W8AJ68 A0A1Y0Q5W9 A0A0H3GNK2 V0APX2 +1274	Klebsiella pneumoniae (strain 342) Salmonella enterica subsp. enterica serovar Senftenberg str. A4-543 Salmonella choleraesuis Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) Klebsiella pneumoniae IS22 Klebsiella pneumoniae Klebsiella pneumoniae subsp. pneumoniae Klebsiella pneumoniae subsp. pneumoniae (strain HS11286) Klebsiella pneumoniae 909957 Klebsiella pneumoniae 30660/NJST258_1 And more	383	UniRef90_B5Y1I9	Cluster: Lipid-A-disaccharide synthase	1,285

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P10441	P72216 A0A2L0YMC7 A0A1F2JXE6 UPI000669A613 UPI000538AF17 A0A205JA05 A0A2J9L832 UPI00038439E9 UPI000907DA9B UPI000C00074D +1633	Proteus mirabilis Proteus sp. HMSC14B05 Proteus vulgaris Proteus alimentorum Proteus hauseri ZMd44 Shigella sp. FC1967 Shigella sp. FC130 Shigella sp. FC1655 Proteus columbae Proteus mirabilis (strain HI4320) And more	141	UniRef50_P72216	Cluster: Lipid-A-disaccharide synthase (Fragment)	1,644

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M19334 Genomic DNA Translation: AAC36919.1 U70214 Genomic DNA Translation: AAB08611.1 U00096 Genomic DNA Translation: AAC73293.1 AP009048 Genomic DNA Translation: BAA77857.2
PIRⁱ	F64742 SYECLA
NCBI Reference Sequences More...RefSeqⁱ	NP_414724.1, NC_000913.3 WP_000139654.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P10441
SMRⁱ	P10441
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4260870581 interactors.
Database of interacting proteins More...DIPⁱ	DIP-10122N
IntActⁱ	P10441 26 interactors.
STRINGⁱ	316385.ECDH10B_0162

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	GT19 Glycosyltransferase Family 19

CAZyⁱ

GT19 Glycosyltransferase Family 19

Proteomic databases

EPDⁱ	P10441
PaxDbⁱ	P10441
PRIDEⁱ	P10441

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	944838
Structural Biology Knowledgebase	Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC73293; AAC73293; b0182 BAA77857; BAA77857; BAA77857
GeneIDⁱ	944838
KEGGⁱ	ecj:JW0177 eco:b0182
PATRICⁱ	fig\|1411691.4.peg.2097

Organism-specific databases

EchoBASEⁱ	EB0541
EcoGeneⁱ	EG10546 lpxB

Phylogenomic databases

eggNOGⁱ	ENOG4105D0V Bacteria COG0763 LUCA
HOGENOMⁱ	HOG000018003
InParanoidⁱ	P10441
KEGG Orthology (KO) More...KOⁱ	K00748
OMAⁱ	PTVWAWR
PhylomeDBⁱ	P10441

Enzyme and pathway databases

UniPathwayⁱ	UPA00359; UER00481
BioCycⁱ	EcoCyc:LIPIDADISACCHARIDESYNTH-MONOMER MetaCyc:LIPIDADISACCHARIDESYNTH-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P10441

PROⁱ

PR:P10441

Family and domain databases

HAMAPⁱ	MF_00392 LpxB, 1 hit
InterProⁱ	View protein in InterPro IPR003835 Glyco_trans_19
PANTHERⁱ	PTHR30372 PTHR30372, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02684 LpxB, 1 hit
TIGRFAMsⁱ	TIGR00215 lpxB, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	LPXB_ECOLI
Accessionⁱ	P10441Primary (citable) accession number: P10441 Secondary accession number(s): P78298
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
	Last sequence update:	July 19, 2003
	Last modified:	March 28, 2018
	This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P10441 (LPXB_ECOLI)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Lipid-A-disaccharide synthase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: lipid IV(A) biosynthesis

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Your basket is currently empty. ⁱ

Functionⁱ

Pathwayⁱ: lipid IV(A) biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ