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P10441

- LPXB_ECOLI

UniProt

P10441 - LPXB_ECOLI

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Protein
Lipid-A-disaccharide synthase
Gene
lpxB, pgsB, b0182, JW0177
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate = UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. lipid-A-disaccharide synthase activity Source: EcoliWiki
  2. phospholipid binding Source: EcoliWiki
  3. protein binding Source: IntAct

GO - Biological processi

  1. lipid A biosynthetic process Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciEcoCyc:LIPIDADISACCHARIDESYNTH-MONOMER.
ECOL316407:JW0177-MONOMER.
MetaCyc:LIPIDADISACCHARIDESYNTH-MONOMER.
UniPathwayiUPA00359; UER00481.

Protein family/group databases

CAZyiGT19. Glycosyltransferase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid-A-disaccharide synthase (EC:2.4.1.182)
Gene namesi
Name:lpxB
Synonyms:pgsB
Ordered Locus Names:b0182, JW0177
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10546. lpxB.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: EcoCyc
  2. cytosol Source: EcoCyc
  3. extrinsic component of plasma membrane Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 382382Lipid-A-disaccharide synthaseUniRule annotation
PRO_0000190164Add
BLAST

Proteomic databases

PaxDbiP10441.
PRIDEiP10441.

Expressioni

Gene expression databases

GenevestigatoriP10441.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
tufAP0CE472EBI-553692,EBI-301077

Protein-protein interaction databases

DIPiDIP-10122N.
IntActiP10441. 25 interactions.
MINTiMINT-1246245.
STRINGi511145.b0182.

Structurei

3D structure databases

ProteinModelPortaliP10441.

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxB family.

Phylogenomic databases

eggNOGiCOG0763.
HOGENOMiHOG000018003.
KOiK00748.
OMAiVSPITYR.
OrthoDBiEOG6FBWZR.
PhylomeDBiP10441.

Family and domain databases

HAMAPiMF_00392. LpxB.
InterProiIPR003835. Glyco_trans_19.
[Graphical view]
PfamiPF02684. LpxB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00215. lpxB. 1 hit.

Sequencei

Sequence statusi: Complete.

P10441-1 [UniParc]FASTAAdd to Basket

« Hide

MTEQRPLTIA LVAGETSGDI LGAGLIRALK EHVPNARFVG VAGPRMQAEG    50
CEAWYEMEEL AVMGIVEVLG RLRRLLHIRA DLTKRFGELK PDVFVGIDAP 100
DFNITLEGNL KKQGIKTIHY VSPSVWAWRQ KRVFKIGRAT DLVLAFLPFE 150
KAFYDKYNVP CRFIGHTMAD AMPLDPDKNA ARDVLGIPHD AHCLALLPGS 200
RGAEVEMLSA DFLKTAQLLR QTYPDLEIVV PLVNAKRREQ FERIKAEVAP 250
DLSVHLLDGM GREAMVASDA ALLASGTAAL ECMLAKCPMV VGYRMKPFTF 300
WLAKRLVKTD YVSLPNLLAG RELVKELLQE ECEPQKLAAA LLPLLANGKT 350
SHAMHDTFRE LHQQIRCNAD EQAAQAVLEL AQ 382
Length:382
Mass (Da):42,382
Last modified:July 19, 2003 - v2
Checksum:i12C76B6ECA438B47
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti149 – 1491F → V1 Publication
Sequence conflicti149 – 1491F → V1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M19334 Genomic DNA. Translation: AAC36919.1.
U70214 Genomic DNA. Translation: AAB08611.1.
U00096 Genomic DNA. Translation: AAC73293.1.
AP009048 Genomic DNA. Translation: BAA77857.2.
PIRiF64742. SYECLA.
RefSeqiNP_414724.1. NC_000913.3.
YP_488484.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73293; AAC73293; b0182.
BAA77857; BAA77857; BAA77857.
GeneIDi12931876.
944838.
KEGGiecj:Y75_p0178.
eco:b0182.
PATRICi32115475. VBIEscCol129921_0189.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M19334 Genomic DNA. Translation: AAC36919.1 .
U70214 Genomic DNA. Translation: AAB08611.1 .
U00096 Genomic DNA. Translation: AAC73293.1 .
AP009048 Genomic DNA. Translation: BAA77857.2 .
PIRi F64742. SYECLA.
RefSeqi NP_414724.1. NC_000913.3.
YP_488484.1. NC_007779.1.

3D structure databases

ProteinModelPortali P10441.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-10122N.
IntActi P10441. 25 interactions.
MINTi MINT-1246245.
STRINGi 511145.b0182.

Protein family/group databases

CAZyi GT19. Glycosyltransferase Family 19.

Proteomic databases

PaxDbi P10441.
PRIDEi P10441.

Protocols and materials databases

DNASUi 944838.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73293 ; AAC73293 ; b0182 .
BAA77857 ; BAA77857 ; BAA77857 .
GeneIDi 12931876.
944838.
KEGGi ecj:Y75_p0178.
eco:b0182.
PATRICi 32115475. VBIEscCol129921_0189.

Organism-specific databases

EchoBASEi EB0541.
EcoGenei EG10546. lpxB.

Phylogenomic databases

eggNOGi COG0763.
HOGENOMi HOG000018003.
KOi K00748.
OMAi VSPITYR.
OrthoDBi EOG6FBWZR.
PhylomeDBi P10441.

Enzyme and pathway databases

UniPathwayi UPA00359 ; UER00481 .
BioCyci EcoCyc:LIPIDADISACCHARIDESYNTH-MONOMER.
ECOL316407:JW0177-MONOMER.
MetaCyc:LIPIDADISACCHARIDESYNTH-MONOMER.

Miscellaneous databases

PROi P10441.

Gene expression databases

Genevestigatori P10441.

Family and domain databases

HAMAPi MF_00392. LpxB.
InterProi IPR003835. Glyco_trans_19.
[Graphical view ]
Pfami PF02684. LpxB. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00215. lpxB. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the Escherichia coli gene for lipid A disaccharide synthase."
    Crowell D.N., Reznikoff W.S., Raetz C.R.H.
    J. Bacteriol. 169:5727-5734(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region."
    Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.
    Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 149.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "First committed step of lipid A biosynthesis in Escherichia coli: sequence of the lpxA gene."
    Coleman J., Raetz C.R.H.
    J. Bacteriol. 170:1268-1274(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19.
  7. "Sequence analysis of the Escherichia coli dnaE gene."
    Tomasiewicz H.G., McHenry C.S.
    J. Bacteriol. 169:5735-5744(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 329-382.

Entry informationi

Entry nameiLPXB_ECOLI
AccessioniPrimary (citable) accession number: P10441
Secondary accession number(s): P78298
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 19, 2003
Last modified: June 11, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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