lpxB

Functionⁱ

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.

Catalytic activityⁱ

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.1 Publication

Pathwayⁱ: lipid IV(A) biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:

This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functionⁱ

lipid-A-disaccharide synthase activity
Source: EcoliWiki
Inferred from direct assayⁱ
- 6370995
phospholipid binding
Source: EcoliWiki
Inferred from direct assayⁱ
- 19883124

Complete GO annotation...

GO - Biological processⁱ

lipid A biosynthetic process
Source: EcoliWiki
Inferred from mutant phenotypeⁱ
- 6370995

Complete GO annotation...

Keywordsⁱ

Molecular function	Glycosyltransferase, Transferase
Biological process	Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCycⁱ	EcoCyc:LIPIDADISACCHARIDESYNTH-MONOMER. MetaCyc:LIPIDADISACCHARIDESYNTH-MONOMER.
UniPathwayⁱ	UPA00359; UER00481.

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	GT19. Glycosyltransferase Family 19.

CAZyⁱ

GT19. Glycosyltransferase Family 19.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Lipid-A-disaccharide synthase (EC:2.4.1.1821 Publication)
Gene namesⁱ	Name:lpxB Synonyms:pgsB Ordered Locus Names:b0182, JW0177
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10546. lpxB.

EcoGeneⁱ

EG10546. lpxB.

Subcellular locationⁱ

GO - Cellular componentⁱ

cytoplasm
Source: EcoCyc
Inferred from direct assayⁱ
- 6370995
cytosol
Source: EcoCyc
Inferred from direct assayⁱ
- 6370995
extrinsic component of plasma membrane
Source: EcoliWiki
Inferred from direct assayⁱ
- 19883124

Complete GO annotation...

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000190164	1 – 382	Lipid-A-disaccharide synthaseAdd BLAST		382

Proteomic databases

PaxDbⁱ	P10441.
PRIDEⁱ	P10441.

Interactionⁱ

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
tufA	P0CE47	2	EBI-553692,EBI-301077

With

Entry

#Exp.

IntAct

Notes

tufA

P0CE47

2

EBI-553692,EBI-301077

Protein-protein interaction databases

BioGridⁱ	4260870. 553 interactors.
Database of interacting proteins More...DIPⁱ	DIP-10122N.
IntActⁱ	P10441. 25 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-1246245.
STRINGⁱ	316385.ECDH10B_0162.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P10441.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the LpxB family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105D0V. Bacteria. COG0763. LUCA.
HOGENOMⁱ	HOG000018003.
InParanoidⁱ	P10441.
KEGG Orthology (KO) More...KOⁱ	K00748.
PhylomeDBⁱ	P10441.

Family and domain databases

HAMAPⁱ	MF_00392. LpxB. 1 hit.
InterProⁱ	View protein in InterPro IPR003835. Glyco_trans_19.
PANTHERⁱ	PTHR30372. PTHR30372. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02684. LpxB. 1 hit.
TIGRFAMsⁱ	TIGR00215. lpxB. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

P10441-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTEQRPLTIA LVAGETSGDI LGAGLIRALK EHVPNARFVG VAGPRMQAEG 
        60         70         80         90        100
CEAWYEMEEL AVMGIVEVLG RLRRLLHIRA DLTKRFGELK PDVFVGIDAP 
       110        120        130        140        150
DFNITLEGNL KKQGIKTIHY VSPSVWAWRQ KRVFKIGRAT DLVLAFLPFE 
       160        170        180        190        200
KAFYDKYNVP CRFIGHTMAD AMPLDPDKNA ARDVLGIPHD AHCLALLPGS 
       210        220        230        240        250
RGAEVEMLSA DFLKTAQLLR QTYPDLEIVV PLVNAKRREQ FERIKAEVAP 
       260        270        280        290        300
DLSVHLLDGM GREAMVASDA ALLASGTAAL ECMLAKCPMV VGYRMKPFTF 
       310        320        330        340        350
WLAKRLVKTD YVSLPNLLAG RELVKELLQE ECEPQKLAAA LLPLLANGKT 
       360        370        380 
SHAMHDTFRE LHQQIRCNAD EQAAQAVLEL AQ

Length:382

Mass (Da):42,382

Last modified:July 19, 2003 - v2

Checksum:ⁱ12C76B6ECA438B47

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	149	F → V (PubMed:2824445).Curated		1
Sequence conflictⁱ	149	F → V (Ref. 2) Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M19334 Genomic DNA. Translation: AAC36919.1. U70214 Genomic DNA. Translation: AAB08611.1. U00096 Genomic DNA. Translation: AAC73293.1. AP009048 Genomic DNA. Translation: BAA77857.2.
PIRⁱ	F64742. SYECLA.
NCBI Reference Sequences More...RefSeqⁱ	NP_414724.1. NC_000913.3. WP_000139654.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaⁱ	AAC73293; AAC73293; b0182. BAA77857; BAA77857; BAA77857.
GeneIDⁱ	944838.
KEGGⁱ	ecj:JW0177. eco:b0182.
PATRICⁱ	fig\|1411691.4.peg.2097.

Similar proteinsⁱ

Entry	Cluster members	Organisms	Length	Cluster ID	Cluster name	Size
P10441	A7ZHS2 C4ZRS4 B1XD51 A7ZWC8 Q3Z5H6 A0A1R1UE70 A0A0H7K682 Q0P6M4 F4SFI6 A0A1X3JNM9 +24	Escherichia coli O139:H28 (strain E24377A / ETEC) Escherichia coli (strain K12 / MC4100 / BW2952) Escherichia coli (strain K12 / DH10B) Escherichia coli O9:H4 (strain HS) Escherichia coli (strain K12) Shigella sonnei (strain Ss046) Escherichia coli N40607 Shigella sonnei Escherichia coli Escherichia coli H736 And more	382	UniRef100_A7ZHS2	Cluster: Lipid-A-disaccharide synthase	35

Entry	Cluster members	Organisms	Length	Cluster ID	Cluster name	Size
P10441	Q8ZRN9 A0A0F7J397 A0A0D6HH03 A0A0W3LU42 A0A0U1GPA0 E8XI27 A0A0H3N9D5 A0A0F6AX33 A0A1W2L842 UPI0008731520 +1056	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) Salmonella enterica I Salmonella typhimurium Salmonella choleraesuis Salmonella enterica subsp. enterica serovar Typhimurium str. DT104 Salmonella typhimurium (strain 4/74) Salmonella typhimurium (strain SL1344) Salmonella typhimurium (strain 14028s / SGSC 2262) Salmonella enterica subsp. enterica serovar Saintpaul str. SARA23 Salmonella enterica subsp. enterica serovar Senftenberg str. A4-543 And more	382	UniRef90_Q8ZRN9	Cluster: Lipid-A-disaccharide synthase	1,067

Entry	Cluster members	Organisms	Length	Cluster ID	Cluster name	Size
P10441	Q8ZRN9 A0A0F7J397 A0A0D6HH03 A0A0W3LU42 A0A0U1GPA0 E8XI27 A0A0H3N9D5 A0A0F6AX33 A0A1W2L842 UPI0008731520 +1149	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) Salmonella enterica I Salmonella typhimurium Salmonella choleraesuis Salmonella enterica subsp. enterica serovar Typhimurium str. DT104 Salmonella typhimurium (strain 4/74) Salmonella typhimurium (strain SL1344) Salmonella typhimurium (strain 14028s / SGSC 2262) Salmonella enterica subsp. enterica serovar Saintpaul str. SARA23 Salmonella enterica subsp. enterica serovar Senftenberg str. A4-543 And more	382	UniRef50_Q8ZRN9	Cluster: Lipid-A-disaccharide synthase	1,160

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M19334 Genomic DNA. Translation: AAC36919.1. U70214 Genomic DNA. Translation: AAB08611.1. U00096 Genomic DNA. Translation: AAC73293.1. AP009048 Genomic DNA. Translation: BAA77857.2.
PIRⁱ	F64742. SYECLA.
NCBI Reference Sequences More...RefSeqⁱ	NP_414724.1. NC_000913.3. WP_000139654.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortalⁱ	P10441.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4260870. 553 interactors.
Database of interacting proteins More...DIPⁱ	DIP-10122N.
IntActⁱ	P10441. 25 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-1246245.
STRINGⁱ	316385.ECDH10B_0162.

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	GT19. Glycosyltransferase Family 19.

CAZyⁱ

GT19. Glycosyltransferase Family 19.

Proteomic databases

PaxDbⁱ	P10441.
PRIDEⁱ	P10441.

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	944838.
Structural Biology Knowledgebase	Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC73293; AAC73293; b0182. BAA77857; BAA77857; BAA77857.
GeneIDⁱ	944838.
KEGGⁱ	ecj:JW0177. eco:b0182.
PATRICⁱ	fig\|1411691.4.peg.2097.

Organism-specific databases

EchoBASEⁱ	EB0541.
EcoGeneⁱ	EG10546. lpxB.

Phylogenomic databases

eggNOGⁱ	ENOG4105D0V. Bacteria. COG0763. LUCA.
HOGENOMⁱ	HOG000018003.
InParanoidⁱ	P10441.
KEGG Orthology (KO) More...KOⁱ	K00748.
PhylomeDBⁱ	P10441.

Enzyme and pathway databases

UniPathwayⁱ	UPA00359; UER00481.
BioCycⁱ	EcoCyc:LIPIDADISACCHARIDESYNTH-MONOMER. MetaCyc:LIPIDADISACCHARIDESYNTH-MONOMER.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P10441.

PROⁱ

PR:P10441.

Family and domain databases

HAMAPⁱ	MF_00392. LpxB. 1 hit.
InterProⁱ	View protein in InterPro IPR003835. Glyco_trans_19.
PANTHERⁱ	PTHR30372. PTHR30372. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02684. LpxB. 1 hit.
TIGRFAMsⁱ	TIGR00215. lpxB. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	LPXB_ECOLI
Accessionⁱ	P10441Primary (citable) accession number: P10441 Secondary accession number(s): P78298
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
	Last sequence update:	July 19, 2003
	Last modified:	August 30, 2017
	This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene
PATHWAY comments
Index of metabolic and biosynthesis pathways
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P10441 (LPXB_ECOLI)

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Lipid-A-disaccharide synthase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: lipid IV(A) biosynthesis

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

Pathwayⁱ: lipid IV(A) biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ