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P10441

- LPXB_ECOLI

UniProt

P10441 - LPXB_ECOLI

Protein

Lipid-A-disaccharide synthase

Gene

lpxB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 2 (19 Jul 2003)
      Previous versions | rss
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    Functioni

    Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.

    Catalytic activityi

    UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate = UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate.

    Pathwayi

    GO - Molecular functioni

    1. lipid-A-disaccharide synthase activity Source: EcoliWiki
    2. phospholipid binding Source: EcoliWiki
    3. protein binding Source: IntAct

    GO - Biological processi

    1. lipid A biosynthetic process Source: EcoliWiki

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

    Enzyme and pathway databases

    BioCyciEcoCyc:LIPIDADISACCHARIDESYNTH-MONOMER.
    ECOL316407:JW0177-MONOMER.
    MetaCyc:LIPIDADISACCHARIDESYNTH-MONOMER.
    UniPathwayiUPA00359; UER00481.

    Protein family/group databases

    CAZyiGT19. Glycosyltransferase Family 19.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipid-A-disaccharide synthase (EC:2.4.1.182)
    Gene namesi
    Name:lpxB
    Synonyms:pgsB
    Ordered Locus Names:b0182, JW0177
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10546. lpxB.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: EcoCyc
    2. cytosol Source: EcoCyc
    3. extrinsic component of plasma membrane Source: EcoCyc

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 382382Lipid-A-disaccharide synthasePRO_0000190164Add
    BLAST

    Proteomic databases

    PaxDbiP10441.
    PRIDEiP10441.

    Expressioni

    Gene expression databases

    GenevestigatoriP10441.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    tufAP0CE472EBI-553692,EBI-301077

    Protein-protein interaction databases

    DIPiDIP-10122N.
    IntActiP10441. 25 interactions.
    MINTiMINT-1246245.
    STRINGi511145.b0182.

    Structurei

    3D structure databases

    ProteinModelPortaliP10441.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LpxB family.Curated

    Phylogenomic databases

    eggNOGiCOG0763.
    HOGENOMiHOG000018003.
    KOiK00748.
    OMAiVSPITYR.
    OrthoDBiEOG6FBWZR.
    PhylomeDBiP10441.

    Family and domain databases

    HAMAPiMF_00392. LpxB.
    InterProiIPR003835. Glyco_trans_19.
    [Graphical view]
    PfamiPF02684. LpxB. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00215. lpxB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P10441-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEQRPLTIA LVAGETSGDI LGAGLIRALK EHVPNARFVG VAGPRMQAEG    50
    CEAWYEMEEL AVMGIVEVLG RLRRLLHIRA DLTKRFGELK PDVFVGIDAP 100
    DFNITLEGNL KKQGIKTIHY VSPSVWAWRQ KRVFKIGRAT DLVLAFLPFE 150
    KAFYDKYNVP CRFIGHTMAD AMPLDPDKNA ARDVLGIPHD AHCLALLPGS 200
    RGAEVEMLSA DFLKTAQLLR QTYPDLEIVV PLVNAKRREQ FERIKAEVAP 250
    DLSVHLLDGM GREAMVASDA ALLASGTAAL ECMLAKCPMV VGYRMKPFTF 300
    WLAKRLVKTD YVSLPNLLAG RELVKELLQE ECEPQKLAAA LLPLLANGKT 350
    SHAMHDTFRE LHQQIRCNAD EQAAQAVLEL AQ 382
    Length:382
    Mass (Da):42,382
    Last modified:July 19, 2003 - v2
    Checksum:i12C76B6ECA438B47
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti149 – 1491F → V(PubMed:2824445)Curated
    Sequence conflicti149 – 1491F → V1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M19334 Genomic DNA. Translation: AAC36919.1.
    U70214 Genomic DNA. Translation: AAB08611.1.
    U00096 Genomic DNA. Translation: AAC73293.1.
    AP009048 Genomic DNA. Translation: BAA77857.2.
    PIRiF64742. SYECLA.
    RefSeqiNP_414724.1. NC_000913.3.
    YP_488484.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73293; AAC73293; b0182.
    BAA77857; BAA77857; BAA77857.
    GeneIDi12931876.
    944838.
    KEGGiecj:Y75_p0178.
    eco:b0182.
    PATRICi32115475. VBIEscCol129921_0189.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M19334 Genomic DNA. Translation: AAC36919.1 .
    U70214 Genomic DNA. Translation: AAB08611.1 .
    U00096 Genomic DNA. Translation: AAC73293.1 .
    AP009048 Genomic DNA. Translation: BAA77857.2 .
    PIRi F64742. SYECLA.
    RefSeqi NP_414724.1. NC_000913.3.
    YP_488484.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P10441.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-10122N.
    IntActi P10441. 25 interactions.
    MINTi MINT-1246245.
    STRINGi 511145.b0182.

    Protein family/group databases

    CAZyi GT19. Glycosyltransferase Family 19.

    Proteomic databases

    PaxDbi P10441.
    PRIDEi P10441.

    Protocols and materials databases

    DNASUi 944838.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73293 ; AAC73293 ; b0182 .
    BAA77857 ; BAA77857 ; BAA77857 .
    GeneIDi 12931876.
    944838.
    KEGGi ecj:Y75_p0178.
    eco:b0182.
    PATRICi 32115475. VBIEscCol129921_0189.

    Organism-specific databases

    EchoBASEi EB0541.
    EcoGenei EG10546. lpxB.

    Phylogenomic databases

    eggNOGi COG0763.
    HOGENOMi HOG000018003.
    KOi K00748.
    OMAi VSPITYR.
    OrthoDBi EOG6FBWZR.
    PhylomeDBi P10441.

    Enzyme and pathway databases

    UniPathwayi UPA00359 ; UER00481 .
    BioCyci EcoCyc:LIPIDADISACCHARIDESYNTH-MONOMER.
    ECOL316407:JW0177-MONOMER.
    MetaCyc:LIPIDADISACCHARIDESYNTH-MONOMER.

    Miscellaneous databases

    PROi P10441.

    Gene expression databases

    Genevestigatori P10441.

    Family and domain databases

    HAMAPi MF_00392. LpxB.
    InterProi IPR003835. Glyco_trans_19.
    [Graphical view ]
    Pfami PF02684. LpxB. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00215. lpxB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the Escherichia coli gene for lipid A disaccharide synthase."
      Crowell D.N., Reznikoff W.S., Raetz C.R.H.
      J. Bacteriol. 169:5727-5734(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region."
      Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.
      Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. "Sequence of minutes 4-25 of Escherichia coli."
      Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 149.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "First committed step of lipid A biosynthesis in Escherichia coli: sequence of the lpxA gene."
      Coleman J., Raetz C.R.H.
      J. Bacteriol. 170:1268-1274(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19.
    7. "Sequence analysis of the Escherichia coli dnaE gene."
      Tomasiewicz H.G., McHenry C.S.
      J. Bacteriol. 169:5735-5744(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 329-382.

    Entry informationi

    Entry nameiLPXB_ECOLI
    AccessioniPrimary (citable) accession number: P10441
    Secondary accession number(s): P78298
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 19, 2003
    Last modified: October 1, 2014
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3