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P10441 (LPXB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipid-A-disaccharide synthase

EC=2.4.1.182
Gene names
Name:lpxB
Synonyms:pgsB
Ordered Locus Names:b0182, JW0177
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length382 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell. HAMAP-Rule MF_00392

Catalytic activity

UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate = UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate. HAMAP-Rule MF_00392

Pathway

Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 5/6. HAMAP-Rule MF_00392

Sequence similarities

Belongs to the LpxB family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

tufAP0CE472EBI-553692,EBI-301077

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 382382Lipid-A-disaccharide synthase HAMAP-Rule MF_00392
PRO_0000190164

Experimental info

Sequence conflict1491F → V Ref.1
Sequence conflict1491F → V Ref.2

Sequences

Sequence LengthMass (Da)Tools
P10441 [UniParc].

Last modified July 19, 2003. Version 2.
Checksum: 12C76B6ECA438B47

FASTA38242,382
        10         20         30         40         50         60 
MTEQRPLTIA LVAGETSGDI LGAGLIRALK EHVPNARFVG VAGPRMQAEG CEAWYEMEEL 

        70         80         90        100        110        120 
AVMGIVEVLG RLRRLLHIRA DLTKRFGELK PDVFVGIDAP DFNITLEGNL KKQGIKTIHY 

       130        140        150        160        170        180 
VSPSVWAWRQ KRVFKIGRAT DLVLAFLPFE KAFYDKYNVP CRFIGHTMAD AMPLDPDKNA 

       190        200        210        220        230        240 
ARDVLGIPHD AHCLALLPGS RGAEVEMLSA DFLKTAQLLR QTYPDLEIVV PLVNAKRREQ 

       250        260        270        280        290        300 
FERIKAEVAP DLSVHLLDGM GREAMVASDA ALLASGTAAL ECMLAKCPMV VGYRMKPFTF 

       310        320        330        340        350        360 
WLAKRLVKTD YVSLPNLLAG RELVKELLQE ECEPQKLAAA LLPLLANGKT SHAMHDTFRE 

       370        380 
LHQQIRCNAD EQAAQAVLEL AQ 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the Escherichia coli gene for lipid A disaccharide synthase."
Crowell D.N., Reznikoff W.S., Raetz C.R.H.
J. Bacteriol. 169:5727-5734(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region."
Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.
Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 149.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"First committed step of lipid A biosynthesis in Escherichia coli: sequence of the lpxA gene."
Coleman J., Raetz C.R.H.
J. Bacteriol. 170:1268-1274(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19.
[7]"Sequence analysis of the Escherichia coli dnaE gene."
Tomasiewicz H.G., McHenry C.S.
J. Bacteriol. 169:5735-5744(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 329-382.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M19334 Genomic DNA. Translation: AAC36919.1.
U70214 Genomic DNA. Translation: AAB08611.1.
U00096 Genomic DNA. Translation: AAC73293.1.
AP009048 Genomic DNA. Translation: BAA77857.2.
PIRSYECLA. F64742.
RefSeqNP_414724.1. NC_000913.3.
YP_488484.1. NC_007779.1.

3D structure databases

ProteinModelPortalP10441.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-10122N.
IntActP10441. 25 interactions.
MINTMINT-1246245.
STRING511145.b0182.

Protein family/group databases

CAZyGT19. Glycosyltransferase Family 19.

Proteomic databases

PaxDbP10441.
PRIDEP10441.

Protocols and materials databases

DNASU944838.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73293; AAC73293; b0182.
BAA77857; BAA77857; BAA77857.
GeneID12931876.
944838.
KEGGecj:Y75_p0178.
eco:b0182.
PATRIC32115475. VBIEscCol129921_0189.

Organism-specific databases

EchoBASEEB0541.
EcoGeneEG10546. lpxB.

Phylogenomic databases

eggNOGCOG0763.
HOGENOMHOG000018003.
KOK00748.
OMAYIAPQEW.
OrthoDBEOG6FBWZR.
PhylomeDBP10441.
ProtClustDBPRK00025.

Enzyme and pathway databases

BioCycEcoCyc:LIPIDADISACCHARIDESYNTH-MONOMER.
ECOL316407:JW0177-MONOMER.
MetaCyc:LIPIDADISACCHARIDESYNTH-MONOMER.
UniPathwayUPA00359; UER00481.

Gene expression databases

GenevestigatorP10441.

Family and domain databases

HAMAPMF_00392. LpxB.
InterProIPR003835. Glyco_trans_19.
[Graphical view]
PfamPF02684. LpxB. 1 hit.
[Graphical view]
TIGRFAMsTIGR00215. lpxB. 1 hit.
ProtoNetSearch...

Other

PROP10441.

Entry information

Entry nameLPXB_ECOLI
AccessionPrimary (citable) accession number: P10441
Secondary accession number(s): P78298
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 19, 2003
Last modified: April 16, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene