UniProtKB - P10441 (LPXB_ECOLI)
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Protein
Lipid-A-disaccharide synthase
Gene
lpxB
Organism
Escherichia coli (strain K12)
Status
Functioni
Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.
Catalytic activityi
UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.1 Publication
: lipid IV(A) biosynthesis Pathwayi
This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.Proteins known to be involved in the 6 steps of the subpathway in this organism are:
- Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
- UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
- UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase (lpxD)
- UDP-2,3-diacylglucosamine hydrolase (lpxH)
- Lipid-A-disaccharide synthase (lpxB)
- Tetraacyldisaccharide 4'-kinase (lpxK)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.
GO - Molecular functioni
- lipid-A-disaccharide synthase activity Source: EcoliWiki
- phospholipid binding Source: EcoliWiki
GO - Biological processi
- lipid A biosynthetic process Source: EcoliWiki
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism |
Enzyme and pathway databases
BioCyci | EcoCyc:LIPIDADISACCHARIDESYNTH-MONOMER MetaCyc:LIPIDADISACCHARIDESYNTH-MONOMER |
UniPathwayi | UPA00359; UER00481 |
Protein family/group databases
CAZyi | GT19 Glycosyltransferase Family 19 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:lpxB Synonyms:pgsB Ordered Locus Names:b0182, JW0177 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Organism-specific databases
EcoGenei | EG10546 lpxB |
Subcellular locationi
GO - Cellular componenti
- cytoplasm Source: EcoCyc
- cytosol Source: EcoCyc
- extrinsic component of plasma membrane Source: EcoliWiki
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000190164 | 1 – 382 | Lipid-A-disaccharide synthaseAdd BLAST | 382 |
Proteomic databases
EPDi | P10441 |
PaxDbi | P10441 |
PRIDEi | P10441 |
Interactioni
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
tufA | P0CE47 | 3 | EBI-553692,EBI-301077 |
Protein-protein interaction databases
BioGridi | 4260870581 interactors. |
DIPi | DIP-10122N |
IntActi | P10441 26 interactors. |
STRINGi | 316385.ECDH10B_0162 |
Structurei
3D structure databases
ProteinModelPortali | P10441 |
SMRi | P10441 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the LpxB family.Curated
Phylogenomic databases
eggNOGi | ENOG4105D0V Bacteria COG0763 LUCA |
HOGENOMi | HOG000018003 |
InParanoidi | P10441 |
KOi | K00748 |
OMAi | PTVWAWR |
PhylomeDBi | P10441 |
Family and domain databases
HAMAPi | MF_00392 LpxB, 1 hit |
InterProi | View protein in InterPro IPR003835 Glyco_trans_19 |
PANTHERi | PTHR30372 PTHR30372, 1 hit |
Pfami | View protein in Pfam PF02684 LpxB, 1 hit |
TIGRFAMsi | TIGR00215 lpxB, 1 hit |
i Sequence
Sequence statusi: Complete.
P10441-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTEQRPLTIA LVAGETSGDI LGAGLIRALK EHVPNARFVG VAGPRMQAEG
60 70 80 90 100
CEAWYEMEEL AVMGIVEVLG RLRRLLHIRA DLTKRFGELK PDVFVGIDAP
110 120 130 140 150
DFNITLEGNL KKQGIKTIHY VSPSVWAWRQ KRVFKIGRAT DLVLAFLPFE
160 170 180 190 200
KAFYDKYNVP CRFIGHTMAD AMPLDPDKNA ARDVLGIPHD AHCLALLPGS
210 220 230 240 250
RGAEVEMLSA DFLKTAQLLR QTYPDLEIVV PLVNAKRREQ FERIKAEVAP
260 270 280 290 300
DLSVHLLDGM GREAMVASDA ALLASGTAAL ECMLAKCPMV VGYRMKPFTF
310 320 330 340 350
WLAKRLVKTD YVSLPNLLAG RELVKELLQE ECEPQKLAAA LLPLLANGKT
360 370 380
SHAMHDTFRE LHQQIRCNAD EQAAQAVLEL AQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 149 | F → V (PubMed:2824445).Curated | 1 | |
Sequence conflicti | 149 | F → V (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M19334 Genomic DNA Translation: AAC36919.1 U70214 Genomic DNA Translation: AAB08611.1 U00096 Genomic DNA Translation: AAC73293.1 AP009048 Genomic DNA Translation: BAA77857.2 |
PIRi | F64742 SYECLA |
RefSeqi | NP_414724.1, NC_000913.3 WP_000139654.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC73293; AAC73293; b0182 BAA77857; BAA77857; BAA77857 |
GeneIDi | 944838 |
KEGGi | ecj:JW0177 eco:b0182 |
PATRICi | fig|1411691.4.peg.2097 |
Similar proteinsi
Entry informationi
Entry namei | LPXB_ECOLI | |
Accessioni | P10441Primary (citable) accession number: P10441 Secondary accession number(s): P78298 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1989 |
Last sequence update: | July 19, 2003 | |
Last modified: | March 28, 2018 | |
This is version 143 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |