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Protein

Lipid-A-disaccharide synthase

Gene

lpxB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.1 Publication

Pathway:ilipid IV(A) biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC)
  3. UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase (lpxD)
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

  • lipid-A-disaccharide synthase activity Source: EcoliWiki
  • phospholipid binding Source: EcoliWiki

GO - Biological processi

  • lipid A biosynthetic process Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciEcoCyc:LIPIDADISACCHARIDESYNTH-MONOMER.
ECOL316407:JW0177-MONOMER.
MetaCyc:LIPIDADISACCHARIDESYNTH-MONOMER.
UniPathwayiUPA00359; UER00481.

Protein family/group databases

CAZyiGT19. Glycosyltransferase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid-A-disaccharide synthase (EC:2.4.1.1821 Publication)
Gene namesi
Name:lpxB
Synonyms:pgsB
Ordered Locus Names:b0182, JW0177
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10546. lpxB.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoCyc
  • cytosol Source: EcoCyc
  • extrinsic component of plasma membrane Source: EcoliWiki
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 382382Lipid-A-disaccharide synthasePRO_0000190164Add
BLAST

Proteomic databases

PaxDbiP10441.
PRIDEiP10441.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
tufAP0CE472EBI-553692,EBI-301077

Protein-protein interaction databases

DIPiDIP-10122N.
IntActiP10441. 25 interactions.
MINTiMINT-1246245.
STRINGi511145.b0182.

Structurei

3D structure databases

ProteinModelPortaliP10441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxB family.Curated

Phylogenomic databases

eggNOGiCOG0763.
HOGENOMiHOG000018003.
InParanoidiP10441.
KOiK00748.
OMAiTVHFVCP.
OrthoDBiEOG6FBWZR.
PhylomeDBiP10441.

Family and domain databases

HAMAPiMF_00392. LpxB.
InterProiIPR003835. Glyco_trans_19.
[Graphical view]
PfamiPF02684. LpxB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00215. lpxB. 1 hit.

Sequencei

Sequence statusi: Complete.

P10441-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEQRPLTIA LVAGETSGDI LGAGLIRALK EHVPNARFVG VAGPRMQAEG
60 70 80 90 100
CEAWYEMEEL AVMGIVEVLG RLRRLLHIRA DLTKRFGELK PDVFVGIDAP
110 120 130 140 150
DFNITLEGNL KKQGIKTIHY VSPSVWAWRQ KRVFKIGRAT DLVLAFLPFE
160 170 180 190 200
KAFYDKYNVP CRFIGHTMAD AMPLDPDKNA ARDVLGIPHD AHCLALLPGS
210 220 230 240 250
RGAEVEMLSA DFLKTAQLLR QTYPDLEIVV PLVNAKRREQ FERIKAEVAP
260 270 280 290 300
DLSVHLLDGM GREAMVASDA ALLASGTAAL ECMLAKCPMV VGYRMKPFTF
310 320 330 340 350
WLAKRLVKTD YVSLPNLLAG RELVKELLQE ECEPQKLAAA LLPLLANGKT
360 370 380
SHAMHDTFRE LHQQIRCNAD EQAAQAVLEL AQ
Length:382
Mass (Da):42,382
Last modified:July 19, 2003 - v2
Checksum:i12C76B6ECA438B47
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti149 – 1491F → V (PubMed:2824445).Curated
Sequence conflicti149 – 1491F → V (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19334 Genomic DNA. Translation: AAC36919.1.
U70214 Genomic DNA. Translation: AAB08611.1.
U00096 Genomic DNA. Translation: AAC73293.1.
AP009048 Genomic DNA. Translation: BAA77857.2.
PIRiF64742. SYECLA.
RefSeqiNP_414724.1. NC_000913.3.
WP_000139654.1. NZ_CP010445.1.

Genome annotation databases

EnsemblBacteriaiAAC73293; AAC73293; b0182.
BAA77857; BAA77857; BAA77857.
GeneIDi944838.
KEGGieco:b0182.
PATRICi32115475. VBIEscCol129921_0189.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19334 Genomic DNA. Translation: AAC36919.1.
U70214 Genomic DNA. Translation: AAB08611.1.
U00096 Genomic DNA. Translation: AAC73293.1.
AP009048 Genomic DNA. Translation: BAA77857.2.
PIRiF64742. SYECLA.
RefSeqiNP_414724.1. NC_000913.3.
WP_000139654.1. NZ_CP010445.1.

3D structure databases

ProteinModelPortaliP10441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-10122N.
IntActiP10441. 25 interactions.
MINTiMINT-1246245.
STRINGi511145.b0182.

Protein family/group databases

CAZyiGT19. Glycosyltransferase Family 19.

Proteomic databases

PaxDbiP10441.
PRIDEiP10441.

Protocols and materials databases

DNASUi944838.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73293; AAC73293; b0182.
BAA77857; BAA77857; BAA77857.
GeneIDi944838.
KEGGieco:b0182.
PATRICi32115475. VBIEscCol129921_0189.

Organism-specific databases

EchoBASEiEB0541.
EcoGeneiEG10546. lpxB.

Phylogenomic databases

eggNOGiCOG0763.
HOGENOMiHOG000018003.
InParanoidiP10441.
KOiK00748.
OMAiTVHFVCP.
OrthoDBiEOG6FBWZR.
PhylomeDBiP10441.

Enzyme and pathway databases

UniPathwayiUPA00359; UER00481.
BioCyciEcoCyc:LIPIDADISACCHARIDESYNTH-MONOMER.
ECOL316407:JW0177-MONOMER.
MetaCyc:LIPIDADISACCHARIDESYNTH-MONOMER.

Miscellaneous databases

PROiP10441.

Family and domain databases

HAMAPiMF_00392. LpxB.
InterProiIPR003835. Glyco_trans_19.
[Graphical view]
PfamiPF02684. LpxB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00215. lpxB. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the Escherichia coli gene for lipid A disaccharide synthase."
    Crowell D.N., Reznikoff W.S., Raetz C.R.H.
    J. Bacteriol. 169:5727-5734(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region."
    Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.
    Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 149.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "First committed step of lipid A biosynthesis in Escherichia coli: sequence of the lpxA gene."
    Coleman J., Raetz C.R.H.
    J. Bacteriol. 170:1268-1274(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19.
  7. "Sequence analysis of the Escherichia coli dnaE gene."
    Tomasiewicz H.G., McHenry C.S.
    J. Bacteriol. 169:5735-5744(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 329-382.
  8. "The biosynthesis of gram-negative endotoxin. Formation of lipid A disaccharides from monosaccharide precursors in extracts of Escherichia coli."
    Ray B.L., Painter G., Raetz C.R.
    J. Biol. Chem. 259:4852-4859(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY.

Entry informationi

Entry nameiLPXB_ECOLI
AccessioniPrimary (citable) accession number: P10441
Secondary accession number(s): P78298
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 19, 2003
Last modified: July 22, 2015
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.