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Protein

Metallo-beta-lactamase type 2

Gene
N/A
Organism
Bacillus sp. (strain 170)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Confers resistance to the different beta-lactams antibiotics (penicillin, cephalosporin and carbapenem) via the hydrolysis of the beta-lactam ring.By similarity

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.By similarity

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi116Zinc 1; via tele nitrogenBy similarity1
Metal bindingi118Zinc 1; via pros nitrogenBy similarity1
Metal bindingi120Zinc 2By similarity1
Metal bindingi179Zinc 1; via tele nitrogenBy similarity1
Metal bindingi198Zinc 2By similarity1
Binding sitei201SubstrateBy similarity1
Binding sitei210Substrate; via amide nitrogenBy similarity1
Metal bindingi240Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Metallo-beta-lactamase type 2Curated (EC:3.5.2.6By similarity)
Alternative name(s):
B2 metallo-beta-lactamaseCurated
Beta-lactamase II1 Publication
CephalosporinaseBy similarity
Metallo-beta-lactamase type II1 Publication
Metallothioprotein beta-lactamase IIBy similarity
Penicillinase1 Publication
Zinc-requiring beta-lactamase IIBy similarity
OrganismiBacillus sp. (strain 170)
Taxonomic identifieri74566 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 301 PublicationAdd BLAST30
ChainiPRO_000001694431 – 257Metallo-beta-lactamase type 2Add BLAST227

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP10425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiIPR001018. Beta-lactamase_class-B_CS.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
PROSITEiPS00743. BETA_LACTAMASE_B_1. 1 hit.
PS00744. BETA_LACTAMASE_B_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10425-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKNTLLKVG LCVSLLGTTQ FVSTISSVQA SQKVEQIVIK NETGTISISQ
60 70 80 90 100
LNKNVWVHTE LGYFNGEAVP SNGLVLNTSK GLVLVDSSWD NKLTKELIEM
110 120 130 140 150
VEKKFQKRVT DVIITHAHAD RIGGITALKE RGIKAHSTAL TAELAKKSGY
160 170 180 190 200
EEPLGDLQTV TNLKFGNTKV ETFYPGKGHT EDNIVVWLPQ YQILAGGCLV
210 220 230 240 250
KSAEAKNLGN VADAYVNEWS TSIENMLKRY RNINLVVPGH GKVGDKGLLL

HTLDLLK
Length:257
Mass (Da):28,153
Last modified:July 1, 1989 - v1
Checksum:i23EEEBDC045AE571
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15350 Genomic DNA. Translation: AAA22275.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15350 Genomic DNA. Translation: AAA22275.1.

3D structure databases

ProteinModelPortaliP10425.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiIPR001018. Beta-lactamase_class-B_CS.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
PROSITEiPS00743. BETA_LACTAMASE_B_1. 1 hit.
PS00744. BETA_LACTAMASE_B_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBLA2_BAC17
AccessioniPrimary (citable) accession number: P10425
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 5, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.