Reviewed, UniProtKB/Swiss-Prot P10425 (BLA2_BAC17)

Names and origin

Protein names	Recommended name:     Beta-lactamase 2     EC=3.5.2.6 Alternative name(s):     Beta-lactamase II     Penicillinase
Organism	Bacillus sp. (strain 170)
Taxonomic identifier	74566 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Can hydrolyze carbapenem compounds.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Cofactor	Binds 2 zinc ions per subunit.
Sequence similarities	Belongs to the class-B beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Ref.1
	Chain	31 – 257	227	Beta-lactamase 2		PRO_0000016944
Sites
	Metal binding	116	1	Zinc 1 By similarity
	Metal binding	118	1	Zinc 1 By similarity
	Metal binding	120	1	Zinc 2 By similarity
	Metal binding	179	1	Zinc 1 By similarity
	Metal binding	198	1	Zinc 2 By similarity
	Metal binding	240	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P10425-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 23EEEBDC045AE571	FASTA	257	28,153
	10 20 30 40 50 60 MKKNTLLKVG LCVSLLGTTQ FVSTISSVQA SQKVEQIVIK NETGTISISQ LNKNVWVHTE 70 80 90 100 110 120 LGYFNGEAVP SNGLVLNTSK GLVLVDSSWD NKLTKELIEM VEKKFQKRVT DVIITHAHAD 130 140 150 160 170 180 RIGGITALKE RGIKAHSTAL TAELAKKSGY EEPLGDLQTV TNLKFGNTKV ETFYPGKGHT 190 200 210 220 230 240 EDNIVVWLPQ YQILAGGCLV KSAEAKNLGN VADAYVNEWS TSIENMLKRY RNINLVVPGH 250 GKVGDKGLLL HTLDLLK

References

[1]	"Nucleotide sequence of the beta-lactamase gene of alkalophilic Bacillus sp. strain 170." Kato C., Kudo T., Watanabe K., Horikoshi K. J. Gen. Microbiol. 131:3317-3324(1985) [PubMed: 3938474] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 31-48.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M15350 Genomic DNA. Translation: AAA22275.1.
3D structure databases
HSSP	HSSP built from PDB template 2BC2 based on UniProtKB P04190.
SMR	P10425. Positions 38-247.
ModBase	Search...
Family and domain databases
InterPro	IPR001018. Beta-lactamase_class-B_CS. IPR001279. Blactmase-like. [Graphical view]
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
PROSITE	PS00743. BETA_LACTAMASE_B_1. 1 hit. PS00744. BETA_LACTAMASE_B_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLA2_BAC17

Accession

Primary (citable) accession number: P10425

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)