P10424 (BLA1_BACCE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase 1
EC=3.5.2.6

Alternative name(s):
Beta-lactamase I
Penicillinase

Gene names

Name:

penPC

Organism

Bacillus cereus

Taxonomic identifier

1396 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts preferentially on penicillins.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Subcellular location	Secreted.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 43	43	Ref.1
	Chain	44 – 306	263	Beta-lactamase 1		PRO_0000016971
Regions
	Region	251 – 253	3	Substrate binding By similarity
Sites
	Active site	89	1	Acyl-ester intermediate By similarity
	Active site	185	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P10424 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: AD0FE16E435A4D12	FASTA	306	33,596
	10 20 30 40 50 60 MKNKKMLKIG MCVGILGLSI TSLVTFTGGA LQVEAKEKTG QVKHKNQATH KEFSQLEKKF 70 80 90 100 110 120 DARLGVYAID TGTNQTIAYR PNERFAFAST YKALAAGVLL QQNSTKKLDE VITYTKEDLV 130 140 150 160 170 180 DYSPVTEKHV DTGMTLGEIA EAAVRYSDNT AGNILFHKIG GPKGYEKALR KMGDRVTMSD 190 200 210 220 230 240 RFETELNEAI PGDIRDTSTA KAIARNLKDF TVGNALPHQK RNILTEWMKG NATGDKLIRA 250 260 270 280 290 300 GVPTDWVDAD KSGAGSYGTR NDIAIVWPPN RSPIIIAILS SKDEKEATYD NQLIKEAAEV VIDAIK

References

[1]

"Cloning and sequencing of the beta-lactamase I gene of Bacillus cereus 5/B and its expression in Bacillus subtilis." Wang W., Mezes P.S.F., Yang Y.Q., Blacher R.W., Lampen J.O. J. Bacteriol. 163:487-492(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 44-63. Strain: ATCC 13061 / DSM 6127 / NCIMB 8967 / NCTC 9946 / NRRL B-3537 / 5/B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M12607 Genomic DNA. Translation: AAA22668.1.
PIR	PNBS5B. A23097.
3D structure databases
ProteinModelPortal	P10424.
SMR	P10424. Positions 52-305.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. IPR023650. Beta-lactam_class-A_AS. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLA1_BACCE

Accession

Primary (citable) accession number: P10424

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program