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Protein

Alkaline phosphatase isozyme conversion protein

Gene

iap

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This protein, presumably an aminopeptidase, mediates the conversion of E.coli alkaline phosphatase isozyme 1, to isozymes 2 and 3 by removing, one by one, the two N-terminal arginine residues.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi117 – 1171Zinc 1By similarity
Metal bindingi143 – 1431Zinc 1By similarity
Metal bindingi143 – 1431Zinc 2; catalyticBy similarity
Metal bindingi176 – 1761Zinc 2; catalyticBy similarity
Metal bindingi204 – 2041Zinc 1By similarity

GO - Molecular functioni

  • aminopeptidase activity Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • post-translational protein modification Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG10488-MONOMER.
ECOL316407:JW2723-MONOMER.
MetaCyc:EG10488-MONOMER.

Protein family/group databases

MEROPSiM28.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase isozyme conversion protein (EC:3.4.11.-)
Gene namesi
Name:iap
Ordered Locus Names:b2753, JW2723
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10488. iap.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424CuratedAdd
BLAST
Chaini25 – 345321Alkaline phosphatase isozyme conversion proteinPRO_0000026855Add
BLAST

Proteomic databases

PaxDbiP10423.
PRIDEiP10423.

Interactioni

Protein-protein interaction databases

BioGridi4259465. 14 interactions.
STRINGi511145.b2753.

Structurei

3D structure databases

ProteinModelPortaliP10423.
SMRiP10423. Positions 44-178.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M28 family. M28C subfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108MXK. Bacteria.
COG2234. LUCA.
HOGENOMiHOG000124091.
KOiK09612.
OMAiVEATNWS.
OrthoDBiEOG68DCWW.

Family and domain databases

InterProiIPR007484. Peptidase_M28.
[Graphical view]
PfamiPF04389. Peptidase_M28. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10423-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSALRHRTA ALALGVCFIL PVHASSPKPG DFANTQARHI ATFFPGRMTG
60 70 80 90 100
TPAEMLSADY IRQQFQQMGY RSDIRTFNSR YIYTARDNRK SWHNVTGSTV
110 120 130 140 150
IAAHEGKAPQ QIIIMAHLDT YAPLSDADAD ANLGGLTLQG MDDNAAGLGV
160 170 180 190 200
MLELAERLKN TPTEYGIRFV ATSGEEEGKL GAENLLKRMS DTEKKNTLLV
210 220 230 240 250
INLDNLIVGD KLYFNSGVKT PEAVRKLTRD RALAIARSHG IAATTNPGLN
260 270 280 290 300
KNYPKGTGCC NDAEIFDKAG IAVLSVEATN WNLGNKDGYQ QRAKTPAFPA
310 320 330 340
GNSWHDVRLD NHQHIDKALP GRIERRCRDV MRIMLPLVKE LAKAS
Length:345
Mass (Da):37,920
Last modified:July 1, 1989 - v1
Checksum:i3DC433FB569E912D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18270 Genomic DNA. Translation: AAA24005.1.
U29579 Genomic DNA. Translation: AAA69263.1.
U00096 Genomic DNA. Translation: AAC75795.1.
AP009048 Genomic DNA. Translation: BAE76830.1.
M74586 Genomic DNA. Translation: AAA23644.1.
PIRiA28382. KZEC.
RefSeqiNP_417233.1. NC_000913.3.
WP_000490428.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75795; AAC75795; b2753.
BAE76830; BAE76830; BAE76830.
GeneIDi947215.
KEGGiecj:JW2723.
eco:b2753.
PATRICi32120912. VBIEscCol129921_2848.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18270 Genomic DNA. Translation: AAA24005.1.
U29579 Genomic DNA. Translation: AAA69263.1.
U00096 Genomic DNA. Translation: AAC75795.1.
AP009048 Genomic DNA. Translation: BAE76830.1.
M74586 Genomic DNA. Translation: AAA23644.1.
PIRiA28382. KZEC.
RefSeqiNP_417233.1. NC_000913.3.
WP_000490428.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP10423.
SMRiP10423. Positions 44-178.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259465. 14 interactions.
STRINGi511145.b2753.

Protein family/group databases

MEROPSiM28.005.

Proteomic databases

PaxDbiP10423.
PRIDEiP10423.

Protocols and materials databases

DNASUi947215.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75795; AAC75795; b2753.
BAE76830; BAE76830; BAE76830.
GeneIDi947215.
KEGGiecj:JW2723.
eco:b2753.
PATRICi32120912. VBIEscCol129921_2848.

Organism-specific databases

EchoBASEiEB0483.
EcoGeneiEG10488. iap.

Phylogenomic databases

eggNOGiENOG4108MXK. Bacteria.
COG2234. LUCA.
HOGENOMiHOG000124091.
KOiK09612.
OMAiVEATNWS.
OrthoDBiEOG68DCWW.

Enzyme and pathway databases

BioCyciEcoCyc:EG10488-MONOMER.
ECOL316407:JW2723-MONOMER.
MetaCyc:EG10488-MONOMER.

Miscellaneous databases

PROiP10423.

Family and domain databases

InterProiIPR007484. Peptidase_M28.
[Graphical view]
PfamiPF04389. Peptidase_M28. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product."
    Ishino Y., Shinagawa H., Makino K., Amemura M., Nakata A.
    J. Bacteriol. 169:5429-5433(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "The DNA sequence of the sulfate activation locus from Escherichia coli K-12."
    Leyh T.S., Vogt T.F., Suo Y.
    J. Biol. Chem. 267:10405-10410(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-111.
    Strain: K12.

Entry informationi

Entry nameiIAP_ECOLI
AccessioniPrimary (citable) accession number: P10423
Secondary accession number(s): Q2MA76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: January 20, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.