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P10410

- TOXK_CYBMR

UniProt

P10410 - TOXK_CYBMR

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Protein

Killer toxin HM-1

Gene

HMK

Organism
Cyberlindnera mrakii (Yeast) (Williopsis mrakii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

This toxin kills sensitive strains of yeast. It inhibits beta-1,3-glucan synthesis.

pH dependencei

Stable over a wide pH range.

Temperature dependencei

Thermostable.

GO - Biological processi

  1. cell death Source: InterPro
  2. pathogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Killer toxin HM-1
Gene namesi
Name:HMK
OrganismiCyberlindnera mrakii (Yeast) (Williopsis mrakii)
Taxonomic identifieri1004253 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesPhaffomycetaceaeCyberlindnera

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Propeptidei20 – 37181 PublicationPRO_0000022568Add
BLAST
Chaini38 – 12588Killer toxin HM-1PRO_0000022569Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi45 ↔ 55
Disulfide bondi48 ↔ 109
Disulfide bondi64 ↔ 95
Disulfide bondi86 ↔ 103
Disulfide bondi104 ↔ 110

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1
125
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi42 – 487
Turni50 – 523
Beta strandi73 – 764
Turni79 – 824
Beta strandi85 – 928
Beta strandi99 – 1046
Beta strandi106 – 1116
Beta strandi120 – 1234

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WKTNMR-A38-125[»]
ProteinModelPortaliP10410.
SMRiP10410. Positions 38-124.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10410.

Family & Domainsi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.20.20. 1 hit.
InterProiIPR011024. G_crystallin-rel.
IPR015290. Yeast-kill-tox.
[Graphical view]
PfamiPF09207. Yeast-kill-tox. 1 hit.
[Graphical view]
SUPFAMiSSF49695. SSF49695. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10410-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFSFVYGLT GFLAATSSAL PSEILSTGYE RSALEKRGDG YLIMCKNCDP
60 70 80 90 100
NTGSCDWKQN WNTCVGIGAN VHWMVTGGST DGKQGCATIW EGSGCVGRST
110 120
TMCCPANTCC NINTGFYIRS YRRVE
Length:125
Mass (Da):13,539
Last modified:May 27, 2002 - v2
Checksum:i8EE4BB0479E38925
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13445 Genomic DNA. Translation: BAA02704.1.
PIRiA23618. TZHQK.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13445 Genomic DNA. Translation: BAA02704.1 .
PIRi A23618. TZHQK.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1WKT NMR - A 38-125 [» ]
ProteinModelPortali P10410.
SMRi P10410. Positions 38-124.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P10410.

Family and domain databases

Gene3Di 2.60.20.20. 1 hit.
InterProi IPR011024. G_crystallin-rel.
IPR015290. Yeast-kill-tox.
[Graphical view ]
Pfami PF09207. Yeast-kill-tox. 1 hit.
[Graphical view ]
SUPFAMi SSF49695. SSF49695. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Isolation and nucleotide sequences of the genes encoding killer toxins from Hansenula mrakii and H. saturnus."
    Kimura T., Kitamoto N., Matsuoka K., Nakamura K., Iimura Y., Kito Y.
    Gene 137:265-270(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NBRC 0895 / NCYC 2251 / VTT C-01434.
  2. "Application of monoclonal antibodies to the isolation and characterization of a killer toxin secreted by Hansenula mrakii."
    Yamamoto T., Imai M., Tachibana K., Mayumi M.
    FEBS Lett. 195:253-257(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 38-125.
  3. "Ancestral beta gamma-crystallin precursor structure in a yeast killer toxin."
    Antuch W., Guntert P., Wuethrich K.
    Nat. Struct. Biol. 3:662-665(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 38-125.

Entry informationi

Entry nameiTOXK_CYBMR
AccessioniPrimary (citable) accession number: P10410
Secondary accession number(s): Q00913
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: May 27, 2002
Last modified: October 29, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3