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P10410

- TOXK_CYBMR

UniProt

P10410 - TOXK_CYBMR

Protein

Killer toxin HM-1

Gene

HMK

Organism
Cyberlindnera mrakii (Yeast) (Williopsis mrakii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    This toxin kills sensitive strains of yeast. It inhibits beta-1,3-glucan synthesis.

    pH dependencei

    Stable over a wide pH range.

    Temperature dependencei

    Thermostable.

    GO - Biological processi

    1. cell death Source: InterPro
    2. pathogenesis Source: InterPro

    Keywords - Molecular functioni

    Toxin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Killer toxin HM-1
    Gene namesi
    Name:HMK
    OrganismiCyberlindnera mrakii (Yeast) (Williopsis mrakii)
    Taxonomic identifieri1004253 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesPhaffomycetaceaeCyberlindnera

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Propeptidei20 – 37181 PublicationPRO_0000022568Add
    BLAST
    Chaini38 – 12588Killer toxin HM-1PRO_0000022569Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi45 ↔ 55
    Disulfide bondi48 ↔ 109
    Disulfide bondi64 ↔ 95
    Disulfide bondi86 ↔ 103
    Disulfide bondi104 ↔ 110

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    Secondary structure

    1
    125
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi42 – 487
    Turni50 – 523
    Beta strandi73 – 764
    Turni79 – 824
    Beta strandi85 – 928
    Beta strandi99 – 1046
    Beta strandi106 – 1116
    Beta strandi120 – 1234

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1WKTNMR-A38-125[»]
    ProteinModelPortaliP10410.
    SMRiP10410. Positions 38-124.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP10410.

    Family & Domainsi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.20.20. 1 hit.
    InterProiIPR011024. G_crystallin-rel.
    IPR015290. Yeast-kill-tox.
    [Graphical view]
    PfamiPF09207. Yeast-kill-tox. 1 hit.
    [Graphical view]
    SUPFAMiSSF49695. SSF49695. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P10410-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFSFVYGLT GFLAATSSAL PSEILSTGYE RSALEKRGDG YLIMCKNCDP    50
    NTGSCDWKQN WNTCVGIGAN VHWMVTGGST DGKQGCATIW EGSGCVGRST 100
    TMCCPANTCC NINTGFYIRS YRRVE 125
    Length:125
    Mass (Da):13,539
    Last modified:May 27, 2002 - v2
    Checksum:i8EE4BB0479E38925
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13445 Genomic DNA. Translation: BAA02704.1.
    PIRiA23618. TZHQK.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13445 Genomic DNA. Translation: BAA02704.1 .
    PIRi A23618. TZHQK.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1WKT NMR - A 38-125 [» ]
    ProteinModelPortali P10410.
    SMRi P10410. Positions 38-124.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P10410.

    Family and domain databases

    Gene3Di 2.60.20.20. 1 hit.
    InterProi IPR011024. G_crystallin-rel.
    IPR015290. Yeast-kill-tox.
    [Graphical view ]
    Pfami PF09207. Yeast-kill-tox. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49695. SSF49695. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and nucleotide sequences of the genes encoding killer toxins from Hansenula mrakii and H. saturnus."
      Kimura T., Kitamoto N., Matsuoka K., Nakamura K., Iimura Y., Kito Y.
      Gene 137:265-270(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: NBRC 0895 / NCYC 2251 / VTT C-01434.
    2. "Application of monoclonal antibodies to the isolation and characterization of a killer toxin secreted by Hansenula mrakii."
      Yamamoto T., Imai M., Tachibana K., Mayumi M.
      FEBS Lett. 195:253-257(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 38-125.
    3. "Ancestral beta gamma-crystallin precursor structure in a yeast killer toxin."
      Antuch W., Guntert P., Wuethrich K.
      Nat. Struct. Biol. 3:662-665(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 38-125.

    Entry informationi

    Entry nameiTOXK_CYBMR
    AccessioniPrimary (citable) accession number: P10410
    Secondary accession number(s): Q00913
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: May 27, 2002
    Last modified: October 1, 2014
    This is version 72 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3