P10410 (TOXK_CYBMR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Killer toxin HM-1

Gene names

Name:

HMK

Organism

Cyberlindnera mrakii (Yeast) (Williopsis mrakii)

Taxonomic identifier

1004253 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Phaffomycetaceae › Cyberlindnera

Protein attributes

Sequence length	125 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This toxin kills sensitive strains of yeast. It inhibits beta-1,3-glucan synthesis.
Subunit structure	Monomer.
Subcellular location	Secreted.
Biophysicochemical properties	pH dependence: Stable over a wide pH range. Temperature dependence: Thermostable.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Toxin
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	cell death Inferred from electronic annotation. Source: InterPro pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Potential

Propeptide

20 – 37

18

PRO_0000022568

Chain

38 – 125

88

Killer toxin HM-1

PRO_0000022569

Amino acid modifications

Disulfide bond

45 ↔ 55

Disulfide bond

48 ↔ 109

Disulfide bond

64 ↔ 95

Disulfide bond

86 ↔ 103

Disulfide bond

104 ↔ 110

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

125

Helix Strand Turn

Details...

Beta strand

42 – 48

7

Turn

50 – 52

3

Beta strand

73 – 76

4

Turn

79 – 82

4

Beta strand

85 – 92

8

Beta strand

99 – 104

6

Beta strand

106 – 111

6

Beta strand

120 – 123

4

Sequences

	Sequence		Length	Mass (Da)
	P10410 [UniParc]. Last modified May 27, 2002. Version 2. Checksum: 8EE4BB0479E38925	FASTA	125	13,539
	10 20 30 40 50 60 MKFSFVYGLT GFLAATSSAL PSEILSTGYE RSALEKRGDG YLIMCKNCDP NTGSCDWKQN 70 80 90 100 110 120 WNTCVGIGAN VHWMVTGGST DGKQGCATIW EGSGCVGRST TMCCPANTCC NINTGFYIRS YRRVE

References

[1]	"Isolation and nucleotide sequences of the genes encoding killer toxins from Hansenula mrakii and H. saturnus." Kimura T., Kitamoto N., Matsuoka K., Nakamura K., Iimura Y., Kito Y. Gene 137:265-270(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NBRC 0895 / NCYC 2251 / VTT C-01434.
[2]	"Application of monoclonal antibodies to the isolation and characterization of a killer toxin secreted by Hansenula mrakii." Yamamoto T., Imai M., Tachibana K., Mayumi M. FEBS Lett. 195:253-257(1986) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 38-125.
[3]	"Ancestral beta gamma-crystallin precursor structure in a yeast killer toxin." Antuch W., Guntert P., Wuethrich K. Nat. Struct. Biol. 3:662-665(1996) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 38-125.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D13445 Genomic DNA. Translation: BAA02704.1.

PIR

TZHQK. A23618.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WKT	NMR	-	A	38-125	[»]

ProteinModelPortal

P10410.

SMR

P10410. Positions 38-124.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.60.20.20. 1 hit.

InterPro

IPR011024. G_crystallin-rel.
IPR015290. Yeast-kill-tox.
[Graphical view]

Pfam

PF09207. Yeast-kill-tox. 1 hit.
[Graphical view]

SUPFAM

SSF49695. G_crystallin_SF. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P10410.

Entry information

Entry name

TOXK_CYBMR

Accession

Primary (citable) accession number: P10410
Secondary accession number(s): Q00913

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	May 27, 2002
Last modified:	April 3, 2013
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program