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Protein

Protein translocase subunit SecA

Gene

secA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for protein export, interacts with the SecYEG preprotein conducting channel. SecA has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving both as a receptor for the preprotein-SecB complex and as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.1 Publication

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit. Zinc is required for binding of the SecB chaperone to the extreme C-terminus of SecA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi885Zinc1
Metal bindingi887Zinc1
Metal bindingi896Zinc1
Metal bindingi897Zinc1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi102 – 109ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • chaperone-mediated protein folding Source: EcoCyc
  • intracellular protein transmembrane transport Source: EcoliWiki
  • protein import Source: InterPro
  • protein targeting Source: EcoliWiki
  • protein transport Source: EcoliWiki
  • protein transport by the Sec complex Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:SECA.
ECOL316407:JW0096-MONOMER.
BRENDAi3.6.3.B1. 2026.

Protein family/group databases

TCDBi3.A.5.1.1. the general secretory pathway (sec) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein translocase subunit SecA
Gene namesi
Name:secA
Synonyms:azi, pea, prlD
Ordered Locus Names:b0098, JW0096
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10936. secA.

Subcellular locationi

GO - Cellular componenti

  • cell envelope Sec protein transport complex Source: EcoCyc
  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc
  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Essential. Leads to loss of translocation of lipoproteins Lpp and BRP.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001095851 – 901Protein translocase subunit SecAAdd BLAST901

Proteomic databases

EPDiP10408.
PaxDbiP10408.
PRIDEiP10408.

Expressioni

Inductioni

Repressed under conditions of excess protein secretion capacity and derepressed when protein secretion becomes limiting. This is regulated by SecM.

Interactioni

Subunit structurei

Monomer and homodimer; probably dimeric in the cytoplasm. It is not yet clear whether the form that functions in protein insertion is monomeric or dimeric. Part of the essential protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF-YajC and YidC. Makes direct contact with SecY.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-543213,EBI-543213
lamBP029433EBI-543213,EBI-371309
secBP0AG864EBI-543213,EBI-555877
secGP0AG992EBI-543213,EBI-6404248
secYP0AGA24EBI-543213,EBI-761422
ybgCP0A8Z32EBI-543213,EBI-543276

Protein-protein interaction databases

BioGridi4261117. 215 interactors.
849222. 1 interactor.
DIPiDIP-10840N.
IntActiP10408. 77 interactors.
MINTiMINT-1224866.
STRINGi511145.b0098.

Structurei

Secondary structure

1901
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 30Combined sources17
Helixi32 – 37Combined sources6
Helixi40 – 55Combined sources16
Helixi60 – 79Combined sources20
Helixi85 – 95Combined sources11
Beta strandi96 – 101Combined sources6
Helixi108 – 120Combined sources13
Beta strandi122 – 124Combined sources3
Beta strandi127 – 132Combined sources6
Helixi133 – 149Combined sources17
Beta strandi154 – 156Combined sources3
Helixi163 – 171Combined sources9
Beta strandi172 – 178Combined sources7
Helixi179 – 189Combined sources11
Helixi195 – 197Combined sources3
Beta strandi205 – 209Combined sources5
Helixi211 – 214Combined sources4
Turni215 – 220Combined sources6
Beta strandi222 – 227Combined sources6
Helixi235 – 238Combined sources4
Turni239 – 244Combined sources6
Beta strandi250 – 252Combined sources3
Helixi281 – 286Combined sources6
Beta strandi287 – 289Combined sources3
Beta strandi290 – 292Combined sources3
Turni293 – 295Combined sources3
Beta strandi300 – 302Combined sources3
Helixi304 – 312Combined sources9
Turni315 – 317Combined sources3
Beta strandi318 – 320Combined sources3
Turni323 – 325Combined sources3
Beta strandi326 – 329Combined sources4
Beta strandi332 – 336Combined sources5
Turni338 – 340Combined sources3
Beta strandi343 – 346Combined sources4
Turni350 – 352Combined sources3
Helixi353 – 360Combined sources8
Beta strandi370 – 376Combined sources7
Helixi377 – 381Combined sources5
Beta strandi384 – 391Combined sources8
Helixi398 – 405Combined sources8
Beta strandi408 – 411Combined sources4
Beta strandi426 – 430Combined sources5
Helixi431 – 446Combined sources16
Turni447 – 449Combined sources3
Beta strandi452 – 458Combined sources7
Helixi459 – 471Combined sources13
Beta strandi477 – 479Combined sources3
Helixi484 – 492Combined sources9
Turni493 – 495Combined sources3
Beta strandi496 – 498Combined sources3
Beta strandi500 – 505Combined sources6
Helixi519 – 525Combined sources7
Beta strandi526 – 528Combined sources3
Helixi533 – 550Combined sources18
Beta strandi553 – 560Combined sources8
Helixi565 – 572Combined sources8
Helixi577 – 579Combined sources3
Beta strandi582 – 589Combined sources8
Helixi590 – 592Combined sources3
Helixi593 – 596Combined sources4
Beta strandi597 – 599Combined sources3
Helixi601 – 604Combined sources4
Helixi605 – 610Combined sources6
Helixi621 – 668Combined sources48
Helixi673 – 689Combined sources17
Helixi698 – 700Combined sources3
Helixi703 – 714Combined sources12
Helixi720 – 726Combined sources7
Helixi728 – 730Combined sources3
Helixi732 – 754Combined sources23
Helixi756 – 787Combined sources32
Turni788 – 790Combined sources3
Beta strandi791 – 793Combined sources3
Beta strandi795 – 797Combined sources3
Turni799 – 801Combined sources3
Helixi802 – 828Combined sources27
Turni884 – 887Combined sources4
Beta strandi889 – 891Combined sources3
Helixi892 – 896Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TM6NMR-A880-901[»]
2FSFX-ray2.00A/B9-861[»]
2FSGX-ray2.20A/B9-861[»]
2FSHX-ray2.00A/B9-861[»]
2FSIX-ray2.11A/B9-861[»]
2VDANMR-A9-836[»]
3BXZX-ray3.00A/B6-609[»]
ProteinModelPortaliP10408.
SMRiP10408.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10408.

Family & Domainsi

Sequence similaritiesi

Belongs to the SecA family.Curated

Phylogenomic databases

eggNOGiENOG4105CI6. Bacteria.
COG0653. LUCA.
HOGENOMiHOG000218168.
InParanoidiP10408.
KOiK03070.
OMAiIATERHE.
PhylomeDBiP10408.

Family and domain databases

Gene3Di1.10.3060.10. 1 hit.
3.40.50.300. 3 hits.
3.90.1440.10. 1 hit.
HAMAPiMF_01382. SecA. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR004027. SEC_C_motif.
IPR000185. SecA.
IPR020937. SecA_CS.
IPR011115. SecA_DEAD.
IPR014018. SecA_motor_DEAD.
IPR011130. SecA_preprotein_X-link_dom.
IPR011116. SecA_Wing/Scaffold.
[Graphical view]
PfamiPF02810. SEC-C. 1 hit.
PF07517. SecA_DEAD. 1 hit.
PF01043. SecA_PP_bind. 1 hit.
PF07516. SecA_SW. 1 hit.
[Graphical view]
PRINTSiPR00906. SECA.
SMARTiSM00957. SecA_DEAD. 1 hit.
SM00958. SecA_PP_bind. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
SSF81767. SSF81767. 1 hit.
SSF81886. SSF81886. 2 hits.
TIGRFAMsiTIGR00963. secA. 1 hit.
PROSITEiPS01312. SECA. 1 hit.
PS51196. SECA_MOTOR_DEAD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10408-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIKLLTKVF GSRNDRTLRR MRKVVNIINA MEPEMEKLSD EELKGKTAEF
60 70 80 90 100
RARLEKGEVL ENLIPEAFAV VREASKRVFG MRHFDVQLLG GMVLNERCIA
110 120 130 140 150
EMRTGEGKTL TATLPAYLNA LTGKGVHVVT VNDYLAQRDA ENNRPLFEFL
160 170 180 190 200
GLTVGINLPG MPAPAKREAY AADITYGTNN EYGFDYLRDN MAFSPEERVQ
210 220 230 240 250
RKLHYALVDE VDSILIDEAR TPLIISGPAE DSSEMYKRVN KIIPHLIRQE
260 270 280 290 300
KEDSETFQGE GHFSVDEKSR QVNLTERGLV LIEELLVKEG IMDEGESLYS
310 320 330 340 350
PANIMLMHHV TAALRAHALF TRDVDYIVKD GEVIIVDEHT GRTMQGRRWS
360 370 380 390 400
DGLHQAVEAK EGVQIQNENQ TLASITFQNY FRLYEKLAGM TGTADTEAFE
410 420 430 440 450
FSSIYKLDTV VVPTNRPMIR KDLPDLVYMT EAEKIQAIIE DIKERTAKGQ
460 470 480 490 500
PVLVGTISIE KSELVSNELT KAGIKHNVLN AKFHANEAAI VAQAGYPAAV
510 520 530 540 550
TIATNMAGRG TDIVLGGSWQ AEVAALENPT AEQIEKIKAD WQVRHDAVLE
560 570 580 590 600
AGGLHIIGTE RHESRRIDNQ LRGRSGRQGD AGSSRFYLSM EDALMRIFAS
610 620 630 640 650
DRVSGMMRKL GMKPGEAIEH PWVTKAIANA QRKVESRNFD IRKQLLEYDD
660 670 680 690 700
VANDQRRAIY SQRNELLDVS DVSETINSIR EDVFKATIDA YIPPQSLEEM
710 720 730 740 750
WDIPGLQERL KNDFDLDLPI AEWLDKEPEL HEETLRERIL AQSIEVYQRK
760 770 780 790 800
EEVVGAEMMR HFEKGVMLQT LDSLWKEHLA AMDYLRQGIH LRGYAQKDPK
810 820 830 840 850
QEYKRESFSM FAAMLESLKY EVISTLSKVQ VRMPEEVEEL EQQRRMEAER
860 870 880 890 900
LAQMQQLSHQ DDDSAAAAAL AAQTGERKVG RNDPCPCGSG KKYKQCHGRL

Q
Length:901
Mass (Da):102,023
Last modified:November 1, 1997 - v2
Checksum:i683D66DD4305DBC2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19R → G AA sequence (PubMed:2841285).Curated1
Sequence conflicti737 – 738ER → DG in AAA24619 (PubMed:2841285).Curated2
Sequence conflicti737 – 738ER → DG in CAA38875 (PubMed:1630901).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20791 Genomic DNA. Translation: AAA24619.1.
M19211 Genomic DNA. Translation: AAA83851.1.
X55034 Genomic DNA. Translation: CAA38875.1.
U00096 Genomic DNA. Translation: AAC73209.1.
AP009048 Genomic DNA. Translation: BAB96666.2.
PIRiB64732. BVECCA.
RefSeqiNP_414640.1. NC_000913.3.
WP_000905789.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73209; AAC73209; b0098.
BAB96666; BAB96666; BAB96666.
GeneIDi944821.
KEGGiecj:JW0096.
eco:b0098.
PATRICi32115301. VBIEscCol129921_0102.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20791 Genomic DNA. Translation: AAA24619.1.
M19211 Genomic DNA. Translation: AAA83851.1.
X55034 Genomic DNA. Translation: CAA38875.1.
U00096 Genomic DNA. Translation: AAC73209.1.
AP009048 Genomic DNA. Translation: BAB96666.2.
PIRiB64732. BVECCA.
RefSeqiNP_414640.1. NC_000913.3.
WP_000905789.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TM6NMR-A880-901[»]
2FSFX-ray2.00A/B9-861[»]
2FSGX-ray2.20A/B9-861[»]
2FSHX-ray2.00A/B9-861[»]
2FSIX-ray2.11A/B9-861[»]
2VDANMR-A9-836[»]
3BXZX-ray3.00A/B6-609[»]
ProteinModelPortaliP10408.
SMRiP10408.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261117. 215 interactors.
849222. 1 interactor.
DIPiDIP-10840N.
IntActiP10408. 77 interactors.
MINTiMINT-1224866.
STRINGi511145.b0098.

Protein family/group databases

TCDBi3.A.5.1.1. the general secretory pathway (sec) family.

Proteomic databases

EPDiP10408.
PaxDbiP10408.
PRIDEiP10408.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73209; AAC73209; b0098.
BAB96666; BAB96666; BAB96666.
GeneIDi944821.
KEGGiecj:JW0096.
eco:b0098.
PATRICi32115301. VBIEscCol129921_0102.

Organism-specific databases

EchoBASEiEB0929.
EcoGeneiEG10936. secA.

Phylogenomic databases

eggNOGiENOG4105CI6. Bacteria.
COG0653. LUCA.
HOGENOMiHOG000218168.
InParanoidiP10408.
KOiK03070.
OMAiIATERHE.
PhylomeDBiP10408.

Enzyme and pathway databases

BioCyciEcoCyc:SECA.
ECOL316407:JW0096-MONOMER.
BRENDAi3.6.3.B1. 2026.

Miscellaneous databases

EvolutionaryTraceiP10408.
PROiP10408.

Family and domain databases

Gene3Di1.10.3060.10. 1 hit.
3.40.50.300. 3 hits.
3.90.1440.10. 1 hit.
HAMAPiMF_01382. SecA. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR004027. SEC_C_motif.
IPR000185. SecA.
IPR020937. SecA_CS.
IPR011115. SecA_DEAD.
IPR014018. SecA_motor_DEAD.
IPR011130. SecA_preprotein_X-link_dom.
IPR011116. SecA_Wing/Scaffold.
[Graphical view]
PfamiPF02810. SEC-C. 1 hit.
PF07517. SecA_DEAD. 1 hit.
PF01043. SecA_PP_bind. 1 hit.
PF07516. SecA_SW. 1 hit.
[Graphical view]
PRINTSiPR00906. SECA.
SMARTiSM00957. SecA_DEAD. 1 hit.
SM00958. SecA_PP_bind. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
SSF81767. SSF81767. 1 hit.
SSF81886. SSF81886. 2 hits.
TIGRFAMsiTIGR00963. secA. 1 hit.
PROSITEiPS01312. SECA. 1 hit.
PS51196. SECA_MOTOR_DEAD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSECA_ECOLI
AccessioniPrimary (citable) accession number: P10408
Secondary accession number(s): P75642
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 165 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.