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Protein

Long-chain fatty acid transport protein

Gene

fadL

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in translocation of long-chain fatty acids across the outer membrane. It is a receptor for the bacteriophage T2. FadL may form a specific channel.

GO - Molecular functioni

  • ligand-gated channel activity Source: EcoCyc
  • long-chain fatty acid transporting porin activity Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Lipid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG10280-MONOMER.
ECOL316407:JW2341-MONOMER.
MetaCyc:EG10280-MONOMER.

Protein family/group databases

TCDBi1.B.9.1.1. the fadl outer membrane protein (fadl) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain fatty acid transport protein
Alternative name(s):
Outer membrane FadL protein
Outer membrane flp protein
Gene namesi
Name:fadL
Synonyms:ttr
Ordered Locus Names:b2344, JW2341
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10280. fadL.

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 251 PublicationAdd BLAST25
ChainiPRO_000002520226 – 446Long-chain fatty acid transport proteinAdd BLAST421

Proteomic databases

EPDiP10384.
PaxDbiP10384.
PRIDEiP10384.

Expressioni

Inductioni

By long-chain fatty acids. Expression of fadL is under the control of the FadR repressor.

Interactioni

Subunit structurei

Has been isolated from outer membrane preparation as a homodimer.

Protein-protein interaction databases

BioGridi4260536. 111 interactors.
STRINGi511145.b2344.

Structurei

Secondary structure

1446
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi35 – 39Combined sources5
Turni40 – 42Combined sources3
Turni45 – 47Combined sources3
Helixi52 – 56Combined sources5
Helixi59 – 64Combined sources6
Beta strandi69 – 84Combined sources16
Beta strandi90 – 92Combined sources3
Beta strandi95 – 97Combined sources3
Beta strandi102 – 112Combined sources11
Beta strandi114 – 124Combined sources11
Beta strandi127 – 132Combined sources6
Turni138 – 142Combined sources5
Beta strandi144 – 160Combined sources17
Beta strandi162 – 183Combined sources22
Helixi187 – 197Combined sources11
Helixi199 – 202Combined sources4
Helixi204 – 214Combined sources11
Beta strandi221 – 243Combined sources23
Beta strandi246 – 253Combined sources8
Beta strandi256 – 260Combined sources5
Beta strandi263 – 265Combined sources3
Turni272 – 276Combined sources5
Beta strandi291 – 296Combined sources6
Beta strandi298 – 309Combined sources12
Beta strandi312 – 325Combined sources14
Beta strandi329 – 334Combined sources6
Beta strandi337 – 342Combined sources6
Beta strandi347 – 359Combined sources13
Beta strandi361 – 374Combined sources14
Helixi379 – 381Combined sources3
Beta strandi384 – 386Combined sources3
Beta strandi391 – 417Combined sources27
Beta strandi420 – 424Combined sources5
Beta strandi427 – 446Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T16X-ray2.60A/B26-446[»]
1T1LX-ray2.80A/B26-446[»]
2R4LX-ray3.30A/B/C26-446[»]
2R4NX-ray3.20A/B26-446[»]
2R4OX-ray3.60A/B26-446[»]
2R4PX-ray2.90A/B26-446[»]
2R88X-ray2.60A/B26-446[»]
2R89X-ray3.40A/B31-446[»]
2R8AX-ray3.00A/B36-446[»]
3DWNX-ray2.50A/B26-446[»]
3PF1X-ray2.70A/B26-446[»]
3PGRX-ray2.60A26-446[»]
3PGSX-ray1.90A/B26-446[»]
3PGUX-ray1.70A26-446[»]
ProteinModelPortaliP10384.
SMRiP10384.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10384.

Family & Domainsi

Sequence similaritiesi

Belongs to the OmpP1/FadL family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105CKF. Bacteria.
COG2067. LUCA.
HOGENOMiHOG000277333.
InParanoidiP10384.
KOiK06076.
OMAiNRYGFTY.
PhylomeDBiP10384.

Family and domain databases

InterProiIPR005017. OMPP1/FadL/TodX.
[Graphical view]
PfamiPF03349. Toluene_X. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10384-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQKTLFTKS ALAVAVALIS TQAWSAGFQL NEFSSSGLGR AYSGEGAIAD
60 70 80 90 100
DAGNVSRNPA LITMFDRPTF SAGAVYIDPD VNISGTSPSG RSLKADNIAP
110 120 130 140 150
TAWVPNMHFV APINDQFGWG ASITSNYGLA TEFNDTYAGG SVGGTTDLET
160 170 180 190 200
MNLNLSGAYR LNNAWSFGLG FNAVYARAKI ERFAGDLGQL VAGQIMQSPA
210 220 230 240 250
GQTQQGQALA ATANGIDSNT KIAHLNGNQW GFGWNAGILY ELDKNNRYAL
260 270 280 290 300
TYRSEVKIDF KGNYSSDLNR AFNNYGLPIP TATGGATQSG YLTLNLPEMW
310 320 330 340 350
EVSGYNRVDP QWAIHYSLAY TSWSQFQQLK ATSTSGDTLF QKHEGFKDAY
360 370 380 390 400
RIALGTTYYY DDNWTFRTGI AFDDSPVPAQ NRSISIPDQD RFWLSAGTTY
410 420 430 440
AFNKDASVDV GVSYMHGQSV KINEGPYQFE SEGKAWLFGT NFNYAF
Length:446
Mass (Da):48,542
Last modified:December 12, 2006 - v5
Checksum:i9B50C55D82CDB085
GO

Sequence cautioni

The sequence AAA64433 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA16205 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA68630 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti176 – 178ARA → RRP (PubMed:2840553).Curated3
Sequence conflicti176A → V in AAA64433 (PubMed:1987139).Curated1
Sequence conflicti286A → R (PubMed:1987139).Curated1
Sequence conflicti286A → R (PubMed:2840553).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60607 Genomic DNA. Translation: AAA64433.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75404.2.
AP009048 Genomic DNA. Translation: BAA16205.1. Different initiation.
Y00552 Genomic DNA. Translation: CAA68630.1. Different initiation.
PIRiF65007.
RefSeqiNP_416846.2. NC_000913.3.
WP_001295701.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75404; AAC75404; b2344.
BAA16205; BAA16205; BAA16205.
GeneIDi946820.
KEGGiecj:JW2341.
eco:b2344.
PATRICi32120061. VBIEscCol129921_2440.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60607 Genomic DNA. Translation: AAA64433.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75404.2.
AP009048 Genomic DNA. Translation: BAA16205.1. Different initiation.
Y00552 Genomic DNA. Translation: CAA68630.1. Different initiation.
PIRiF65007.
RefSeqiNP_416846.2. NC_000913.3.
WP_001295701.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T16X-ray2.60A/B26-446[»]
1T1LX-ray2.80A/B26-446[»]
2R4LX-ray3.30A/B/C26-446[»]
2R4NX-ray3.20A/B26-446[»]
2R4OX-ray3.60A/B26-446[»]
2R4PX-ray2.90A/B26-446[»]
2R88X-ray2.60A/B26-446[»]
2R89X-ray3.40A/B31-446[»]
2R8AX-ray3.00A/B36-446[»]
3DWNX-ray2.50A/B26-446[»]
3PF1X-ray2.70A/B26-446[»]
3PGRX-ray2.60A26-446[»]
3PGSX-ray1.90A/B26-446[»]
3PGUX-ray1.70A26-446[»]
ProteinModelPortaliP10384.
SMRiP10384.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260536. 111 interactors.
STRINGi511145.b2344.

Protein family/group databases

TCDBi1.B.9.1.1. the fadl outer membrane protein (fadl) family.

Proteomic databases

EPDiP10384.
PaxDbiP10384.
PRIDEiP10384.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75404; AAC75404; b2344.
BAA16205; BAA16205; BAA16205.
GeneIDi946820.
KEGGiecj:JW2341.
eco:b2344.
PATRICi32120061. VBIEscCol129921_2440.

Organism-specific databases

EchoBASEiEB0276.
EcoGeneiEG10280. fadL.

Phylogenomic databases

eggNOGiENOG4105CKF. Bacteria.
COG2067. LUCA.
HOGENOMiHOG000277333.
InParanoidiP10384.
KOiK06076.
OMAiNRYGFTY.
PhylomeDBiP10384.

Enzyme and pathway databases

BioCyciEcoCyc:EG10280-MONOMER.
ECOL316407:JW2341-MONOMER.
MetaCyc:EG10280-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP10384.
PROiP10384.

Family and domain databases

InterProiIPR005017. OMPP1/FadL/TodX.
[Graphical view]
PfamiPF03349. Toluene_X. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFADL_ECOLI
AccessioniPrimary (citable) accession number: P10384
Secondary accession number(s): P77697
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: December 12, 2006
Last modified: November 2, 2016
This is version 150 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.