P10378 (ENTE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Enterobactin synthase component E

Alternative name(s):
Enterochelin synthase E

Including the following 2 domains:

1. 2,3-dihydroxybenzoate-AMP ligase
   EC=2.7.7.58
   Alternative name(s):
   Dihydroxybenzoic acid-activating enzyme
2. S-dihydroxybenzoyltransferase
   EC=2.3.1.-

Gene names

Name:	entE
Ordered Locus Names:	b0594, JW0586

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Activates the carboxylate group of 2,3-dihydroxy-benzoate (2,3-DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate. Then, catalyzes the acylation of holo-EntB with 2,3-DHB adenylate, preparing that molecule for amide bond formation with L-serine. Ref.7
Catalytic activity	ATP + 2,3-dihydroxybenzoate = diphosphate + (2,3-dihydroxybenzoyl)adenylate. (2,3-dihydroxybenzoyl)adenylate + holo-entB = adenosine 5'-monophosphate + acyl-holo-entB.
Pathway	Siderophore biosynthesis; enterobactin biosynthesis.
Subunit structure	Proteins EntB, EntD, EntE, and EntF form a multienzyme complex called enterobactin synthase.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family. EntE subfamily.

Ontologies

Keywords
Biological process	Enterobactin biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Acyltransferase Ligase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	enterobactin biosynthetic process Inferred from mutant phenotype. Source: EcoCyc
Molecular function	(2,3-dihydroxybenzoyl)adenylate synthase activity Inferred from direct assay. Source: EcoCyc ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity Inferred from electronic annotation. Source: UniProtKB-KW transferase activity, transferring acyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 536	536	Enterobactin synthase component E		PRO_0000193075
Experimental info
	Sequence conflict	369 – 378	10	DAEGNPLPQG → ECRRKSTAAR in CAA33158. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P10378 [UniParc]. Last modified November 1, 1997. Version 3. Checksum: F818942DFDD8DC99	FASTA	536	59,112
	10 20 30 40 50 60 MSIPFTRWPE EFARRYREKG YWQDLPLTDI LTRHAASDSI AVIDGERQLS YRELNQAADN 70 80 90 100 110 120 LACSLRRQGI KPGETALVQL GNVAELYITF FALLKLGVAP VLALFSHQRS ELNAYASQIE 130 140 150 160 170 180 PALLIADRQH ALFSGDDFLN TFVTEHSSIR VVQLLNDSGE HNLQDAINHP AEDFTATPSP 190 200 210 220 230 240 ADEVAYFQLS GGTTGTPKLI PRTHNDYYYS VRRSVEICQF TQQTRYLCAI PAAHNYAMSS 250 260 270 280 290 300 PGSLGVFLAG GTVVLAADPS ATLCFPLIEK HQVNVTALVP PAVSLWLQAL IEGESRAQLA 310 320 330 340 350 360 SLKLLQVGGA RLSATLAARI PAEIGCQLQQ VFGMAEGLVN YTRLDDSAEK IIHTQGYPMC 370 380 390 400 410 420 PDDEVWVADA EGNPLPQGEV GRLMTRGPYT FRGYYKSPQH NASAFDANGF YCSGDLISID 430 440 450 460 470 480 PEGYITVQGR EKDQINRGGE KIAAEEIENL LLRHPAVIYA ALVSMEDELM GEKSCAYLVV 490 500 510 520 530 KEPLRAVQVR RFLREQGIAE FKLPDRVECV DSLPLTAVGK VDKKQLRQWL ASRASA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the Escherichia coli entE gene." Staab J.F., Elkins M.F., Earhart C.F. FEMS Microbiol. Lett. 50:15-19(1989) [PubMed: 2525505] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Opacity factor from group A streptococci is an apoproteinase." Elkins M.F., Earhart C.F. FEMS Microbiol. Lett. 56:35-39(1990) [PubMed: 2110093] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-8. Strain: K12.
[6]	"Nucleotide sequence of a cluster of Escherichia coli enterobactin biosynthesis genes: identification of entA and purification of its product 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase." Liu J., Duncan K., Walsh C.T. J. Bacteriol. 171:791-798(1989) [PubMed: 2521622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 393-536.
[7]	"Enterobactin biosynthesis in Escherichia coli: isochorismate lyase (EntB) is a bifunctional enzyme that is phosphopantetheinylated by EntD and then acylated by EntE using ATP and 2,3-dihydroxybenzoate." Gehring A.M., Bradley K.A., Walsh C.T. Biochemistry 36:8495-8503(1997) [PubMed: 9214294] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15058 Genomic DNA. Translation: CAA33158.1. U82598 Genomic DNA. Translation: AAB40794.1. U00096 Genomic DNA. Translation: AAC73695.1. AP009048 Genomic DNA. Translation: BAE76349.1. M24148 Unassigned DNA. Translation: AAA16101.1. M36700 Genomic DNA. Translation: AAA18492.1.
PIR	SYECEB. H64792.
RefSeq	NP_415126.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P10378.
SMR	P10378. Positions 1-536.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9515N.
IntAct	P10378. 8 interactions.
MINT	MINT-1228110.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000003938; EBESCP00000003938; EBESCG00000003223. EBESCT00000016642; EBESCP00000015933; EBESCG00000015701.
GeneID	947426.
GenomeReviews	Gene locus JW0586 in contig AP009048_GR. Gene locus b0594 in contig U00096_GR.
KEGG	ecj:JW0586. eco:b0594.
PATRIC	32116364. VBIEscCol129921_0622.
Organism-specific databases
EchoBASE	EB0259.
EcoGene	EG10263. entE.
Phylogenomic databases
eggNOG	COG1021.
GeneTree	EBGT00050000008803.
HOGENOM	HBG547964.
OMA	LHNASAF.
PhylomeDB	P10378.
ProtClustDB	PRK10946.
Enzyme and pathway databases
BioCyc	EcoCyc:ENTE-MONOMER. MetaCyc:ENTE-MONOMER.
Gene expression databases
Genevestigator	P10378.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. IPR011963. DHB_AMP_lig. [Graphical view]
KO	K02363.
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR02275. DHB_AMP_lig. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ENTE_ECOLI

Accession

Primary (citable) accession number: P10378
Secondary accession number(s): P15049, P77773, Q2MBK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 109 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program