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P10371

- HIS4_ECOLI

UniProt

P10371 - HIS4_ECOLI

Protein

1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Gene

hisA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei7 – 71Proton acceptorBy similarity
    Active sitei129 – 1291Proton donorBy similarity

    GO - Molecular functioni

    1. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity Source: EcoCyc

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: EcoliWiki
    2. histidine biosynthetic process Source: EcoCyc

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Histidine biosynthesis

    Enzyme and pathway databases

    BioCyciEcoCyc:PRIBFAICARPISOM-MONOMER.
    ECOL316407:JW2006-MONOMER.
    MetaCyc:PRIBFAICARPISOM-MONOMER.
    UniPathwayiUPA00031; UER00009.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (EC:5.3.1.16)
    Alternative name(s):
    Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase
    Gene namesi
    Name:hisA
    Ordered Locus Names:b2024, JW2006
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10444. hisA.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2452451-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerasePRO_0000142004Add
    BLAST

    Proteomic databases

    PaxDbiP10371.
    PRIDEiP10371.

    2D gel databases

    SWISS-2DPAGEP10371.

    Expressioni

    Gene expression databases

    GenevestigatoriP10371.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    IntActiP10371. 3 interactions.
    STRINGi511145.b2024.

    Structurei

    3D structure databases

    ProteinModelPortaliP10371.
    SMRiP10371. Positions 2-244.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the HisA/HisF family.Curated

    Phylogenomic databases

    eggNOGiCOG0106.
    HOGENOMiHOG000224614.
    KOiK01814.
    OMAiQRDYGSD.
    OrthoDBiEOG6H1Q3W.
    PhylomeDBiP10371.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01014. HisA.
    InterProiIPR013785. Aldolase_TIM.
    IPR006062. His_biosynth.
    IPR006063. HisA.
    IPR023016. Isoase_HisA.
    IPR011060. RibuloseP-bd_barrel.
    [Graphical view]
    PfamiPF00977. His_biosynth. 1 hit.
    [Graphical view]
    SUPFAMiSSF51366. SSF51366. 1 hit.
    TIGRFAMsiTIGR00007. TIGR00007. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P10371-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIIPALDLID GTVVRLHQGD YGKQRDYGND PLPRLQDYAA QGAEVLHLVD    50
    LTGAKDPAKR QIPLIKTLVA GVNVPVQVGG GVRSEEDVAA LLEAGVARVV 100
    VGSTAVKSQD MVKGWFERFG ADALVLALDV RIDEQGNKQV AVSGWQENSG 150
    VSLEQLVETY LPVGLKHVLC TDISRDGTLA GSNVSLYEEV CARYPQVAFQ 200
    SSGGIGDIDD VAALRGTGVR GVIVGRALLE GKFTVKEAIA CWQNA 245
    Length:245
    Mass (Da):26,033
    Last modified:November 1, 1997 - v2
    Checksum:iB21D590E4214EE7E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti84 – 841S → T in CAA31816. (PubMed:3062174)Curated
    Sequence conflicti126 – 1261L → V in CAA31816. (PubMed:3062174)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13462 Genomic DNA. Translation: CAA31816.1.
    U00096 Genomic DNA. Translation: AAC75085.2.
    AP009048 Genomic DNA. Translation: BAA15855.1.
    PIRiG64967. ISECIC.
    RefSeqiNP_416528.2. NC_000913.3.
    YP_490267.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75085; AAC75085; b2024.
    BAA15855; BAA15855; BAA15855.
    GeneIDi12931426.
    946521.
    KEGGiecj:Y75_p1987.
    eco:b2024.
    PATRICi32119383. VBIEscCol129921_2101.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13462 Genomic DNA. Translation: CAA31816.1 .
    U00096 Genomic DNA. Translation: AAC75085.2 .
    AP009048 Genomic DNA. Translation: BAA15855.1 .
    PIRi G64967. ISECIC.
    RefSeqi NP_416528.2. NC_000913.3.
    YP_490267.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P10371.
    SMRi P10371. Positions 2-244.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P10371. 3 interactions.
    STRINGi 511145.b2024.

    2D gel databases

    SWISS-2DPAGE P10371.

    Proteomic databases

    PaxDbi P10371.
    PRIDEi P10371.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75085 ; AAC75085 ; b2024 .
    BAA15855 ; BAA15855 ; BAA15855 .
    GeneIDi 12931426.
    946521.
    KEGGi ecj:Y75_p1987.
    eco:b2024.
    PATRICi 32119383. VBIEscCol129921_2101.

    Organism-specific databases

    EchoBASEi EB0439.
    EcoGenei EG10444. hisA.

    Phylogenomic databases

    eggNOGi COG0106.
    HOGENOMi HOG000224614.
    KOi K01814.
    OMAi QRDYGSD.
    OrthoDBi EOG6H1Q3W.
    PhylomeDBi P10371.

    Enzyme and pathway databases

    UniPathwayi UPA00031 ; UER00009 .
    BioCyci EcoCyc:PRIBFAICARPISOM-MONOMER.
    ECOL316407:JW2006-MONOMER.
    MetaCyc:PRIBFAICARPISOM-MONOMER.

    Miscellaneous databases

    PROi P10371.

    Gene expression databases

    Genevestigatori P10371.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01014. HisA.
    InterProi IPR013785. Aldolase_TIM.
    IPR006062. His_biosynth.
    IPR006063. HisA.
    IPR023016. Isoase_HisA.
    IPR011060. RibuloseP-bd_barrel.
    [Graphical view ]
    Pfami PF00977. His_biosynth. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51366. SSF51366. 1 hit.
    TIGRFAMsi TIGR00007. TIGR00007. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structure and function of the Salmonella typhimurium and Escherichia coli K-12 histidine operons."
      Carlomagno M.S., Chiariotti L., Alifano P., Nappo A.G., Bruni C.B.
      J. Mol. Biol. 203:585-606(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiHIS4_ECOLI
    AccessioniPrimary (citable) accession number: P10371
    Secondary accession number(s): P78078
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3