P10371 (HIS4_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
EC=5.3.1.16

Alternative name(s):
Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Gene names

Name:	hisA
Ordered Locus Names:	b2024, JW2006

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_01014
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9. HAMAP-Rule MF_01014
Subunit structure	Monomer.
Subcellular location	Cytoplasm HAMAP-Rule MF_01014.
Sequence similarities	Belongs to the HisA/HisF family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	histidine biosynthetic process Inferred from direct assay PubMed 12356303. Source: EcoCyc response to DNA damage stimulus Inferred from expression pattern PubMed 11967071. Source: EcoliWiki
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity Inferred from direct assay PubMed 12356303. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase HAMAP-Rule MF_01014		PRO_0000142004
Sites
	Active site	7	1	Proton acceptor By similarity
	Active site	129	1	Proton donor By similarity
Experimental info
	Sequence conflict	84	1	S → T in CAA31816. Ref.1
	Sequence conflict	126	1	L → V in CAA31816. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P10371 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: B21D590E4214EE7E	FASTA	245	26,033
	10 20 30 40 50 60 MIIPALDLID GTVVRLHQGD YGKQRDYGND PLPRLQDYAA QGAEVLHLVD LTGAKDPAKR 70 80 90 100 110 120 QIPLIKTLVA GVNVPVQVGG GVRSEEDVAA LLEAGVARVV VGSTAVKSQD MVKGWFERFG 130 140 150 160 170 180 ADALVLALDV RIDEQGNKQV AVSGWQENSG VSLEQLVETY LPVGLKHVLC TDISRDGTLA 190 200 210 220 230 240 GSNVSLYEEV CARYPQVAFQ SSGGIGDIDD VAALRGTGVR GVIVGRALLE GKFTVKEAIA CWQNA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and function of the Salmonella typhimurium and Escherichia coli K-12 histidine operons." Carlomagno M.S., Chiariotti L., Alifano P., Nappo A.G., Bruni C.B. J. Mol. Biol. 203:585-606(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X13462 Genomic DNA. Translation: CAA31816.1. U00096 Genomic DNA. Translation: AAC75085.2. AP009048 Genomic DNA. Translation: BAA15855.1.
PIR	ISECIC. G64967.
RefSeq	NP_416528.2. NC_000913.2. YP_490267.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P10371.
SMR	P10371. Positions 2-244.
ModBase	Search...
Protein-protein interaction databases
IntAct	P10371. 3 interactions.
STRING	511145.b2024.
2D gel databases
SWISS-2DPAGE	P10371.
Proteomic databases
PaxDb	P10371.
PRIDE	P10371.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75085; AAC75085; b2024. BAA15855; BAA15855; BAA15855.
GeneID	12931426. 946521.
KEGG	ecj:Y75_p1987. eco:b2024.
PATRIC	32119383. VBIEscCol129921_2101.
Organism-specific databases
EchoBASE	EB0439.
EcoGene	EG10444. hisA.
Phylogenomic databases
eggNOG	COG0106.
HOGENOM	HOG000224614.
KO	K01814.
OMA	DGHCVRL.
ProtClustDB	PRK00748.
Enzyme and pathway databases
BioCyc	EcoCyc:PRIBFAICARPISOM-MONOMER. ECOL316407:JW2006-MONOMER. MetaCyc:PRIBFAICARPISOM-MONOMER.
UniPathway	UPA00031; UER00009.
Gene expression databases
Genevestigator	P10371.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01014. HisA.
InterPro	IPR013785. Aldolase_TIM. IPR006062. His_biosynth. IPR006063. HisA. IPR023016. Isoase_HisA. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Pfam	PF00977. His_biosynth. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00007. TIGR00007. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HIS4_ECOLI

Accession

Primary (citable) accession number: P10371
Secondary accession number(s): P78078

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	November 1, 1997
Last modified:	May 29, 2013
This is version 123 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program