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P10363 (PRI1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA primase small subunit

EC=2.7.7.-
Alternative name(s):
DNA polymerase alpha:primase complex p48 subunit
Short name=DNA polymerase-primase complex p48 subunit
Short name=Pol alpha-primase complex p48 subunit
DNA primase 48 kDa subunit
Gene names
Name:PRI1
Ordered Locus Names:YIR008C
ORF Names:YIB8C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length409 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. In a complex with DNA polymerase alpha (DNA polymerase alpha:primase) constitutes a replicative polymerase. Both primase components participate in formation of the active center, but the ATP-binding site is exclusively located on p48.

Subunit structure

DNA polymerase alpha:primase is a four subunit enzyme complex, which is assembled throughout the cell cycle, and consists of the two DNA polymerase subunits A and B, and the DNA primase large and small subunits.

Miscellaneous

The bound zinc ion is not a cofactor. It is bound to a zinc knuckle motif that may be involved in sequence recognition and the binding of ssDNA By similarity.

Present with 197 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the eukaryotic-type primase small subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 409409DNA primase small subunit
PRO_0000046736

Regions

Motif123 – 13311Zinc knuckle motif

Sites

Active site461 Potential
Active site1111 Potential
Active site1131 Potential

Experimental info

Mutagenesis1841R → Q: Temperature-sensitive.
Mutagenesis3161E → K: Cold-sensitive.
Sequence conflict231 – 2322EQ → DE in CAA68513. Ref.1

Secondary structure

......................................................... 409
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P10363 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: C4B1C82257AA86F2

FASTA40947,690
        10         20         30         40         50         60 
MTNSVKTNGP SSSDMEYYYK SLYPFKHIFN WLNHSPKPSR DMINREFAMA FRSGAYKRYN 

        70         80         90        100        110        120 
SFNSVQDFKA QIEKANPDRF EIGAIYNKPP RERDTLLKSE LKALEKELVF DIDMDDYDAF 

       130        140        150        160        170        180 
RTCCSGAQVC SKCWKFISLA MKITNTALRE DFGYKDFIWV FSGRRGAHCW VSDKRARALT 

       190        200        210        220        230        240 
DVQRRNVLDY VNVIRDRNTD KRLALKRPYH PHLARSLEQL KPFFVSIMLE EQNPWEDDQH 

       250        260        270        280        290        300 
AIQTLLPALY DKQLIDSLKK YWLDNPRRSS KEKWNDIDQI ATSLFKGPKQ DSHIIKLREC 

       310        320        330        340        350        360 
KEDLVLMTLY PKLDVEVTKQ TIHLLKAPFC IHPATGNVCV PIDESFAPEK APKLIDLQTE 

       370        380        390        400 
MEKNNDVSLT ALQPFINQFQ AYVSSLLKNE LGSVKRERED DDEPASLDF 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of the PRI1 gene related to DNA primase in Saccharomyces cerevisiae."
Plevani P., Francesconi S., Lucchini G.
Nucleic Acids Res. 15:7975-7989(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DBY939.
[2]"Nucleotide sequence and analysis of the centromeric region of yeast chromosome IX."
Voss H., Tamames J., Teodoru C., Valencia A., Sensen C., Wiemann S., Schwager C., Zimmermann J., Sander C., Ansorge W.
Yeast 11:61-78(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"The yeast DNA polymerase-primase complex: genes and proteins."
Plevani P., Foiani M., Muzi Falconi M., Pizzagalli A., Santocanale C., Francesconi S., Valsasnini P., Comedini A., Piatti S., Lucchini G.
Biochim. Biophys. Acta 951:268-273(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: COMPOSITION OF THE DNA POLYMERASE ALPHA:PRIMASE COMPLEX.
[7]"Mutations in conserved yeast DNA primase domains impair DNA replication in vivo."
Francesconi S., Longhese M.P., Piseri A., Santocanale C., Lucchini G., Plevani P.
Proc. Natl. Acad. Sci. U.S.A. 88:3877-3881(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z37996 Genomic DNA. Translation: CAA86078.1.
X79743 Genomic DNA. Translation: CAB38098.1.
Y00458 Genomic DNA. Translation: CAA68513.1.
AY692859 Genomic DNA. Translation: AAT92878.1.
BK006942 Genomic DNA. Translation: DAA08554.1.
PIRS48352.
RefSeqNP_012273.1. NM_001179530.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4LIMX-ray1.63A8-396[»]
ProteinModelPortalP10363.
SMRP10363. Positions 15-392.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35000. 145 interactions.
DIPDIP-2534N.
IntActP10363. 6 interactions.
MINTMINT-572443.
STRING4932.YIR008C.

Proteomic databases

PaxDbP10363.
PeptideAtlasP10363.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIR008C; YIR008C; YIR008C.
GeneID854825.
KEGGsce:YIR008C.

Organism-specific databases

CYGDYIR008c.
SGDS000001447. PRI1.

Phylogenomic databases

eggNOGCOG1467.
GeneTreeENSGT00390000011466.
HOGENOMHOG000196041.
KOK02684.
OMATHREFAF.
OrthoDBEOG7RJQ25.

Enzyme and pathway databases

BioCycYEAST:G3O-31429-MONOMER.

Gene expression databases

GenevestigatorP10363.

Family and domain databases

InterProIPR002755. DNA_primase_S.
IPR014052. DNA_primase_ssu_euk/arc.
[Graphical view]
PANTHERPTHR10536. PTHR10536. 1 hit.
PfamPF01896. DNA_primase_S. 1 hit.
[Graphical view]
TIGRFAMsTIGR00335. primase_sml. 1 hit.
ProtoNetSearch...

Other

NextBio977680.
PROP10363.

Entry information

Entry namePRI1_YEAST
AccessionPrimary (citable) accession number: P10363
Secondary accession number(s): D6VVT8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references