RNA replicase polyprotein - Turnip yellow mosaic virus

Sequence or UniProt identifier

>sp|P10358|POLR_TYMV RNA replicase polyprotein OS=Turnip yellow mosaic virus PE=1 SV=2 MAFQLALDALAPTTHRDPSLHPILESTVDSIRSSIQTYPWSIPKELLPLLNSYGIPTSGL GTSHHPHAAHKTIETFLLCTHWSFQATTPSSVMFMKPSKFNKLAQVNSNFRELKNYRLHP NDSTRYPFTSPDLPVFPTIFMHDALMYYHPSQIMDLFLRKPNLERLYASLVVPPEAHLSD QSFYPKLYTYTTTRHTLHYVPEGHEAGSYNQPSDAHSWLRINSIRLGNHHLSVTILESWG PVHSLLIQRGTPPPDPSLQAPPTLMTSDLFRSYQEPRLDVVSFRIPDAIELPQATFLQQP LRDRLVPRAVYNALFTYTRAVRTLRTSDPAAFVRMHSSKPDHDWVTSNAWDNLQTFALLN VPLRPNVVYHVLQSPIASLSLYLRQHWRRLTATAVPILSFLTLLQRFLPLPIPLAEVKSI TAFRRELYRKKEPHHPLDVFHLQHRVRNYHSAISAVRPASPPHQKLPHALQKAALLLLRP ISPLLTATPFFRSEQKSMLPNAELSWTLKRFALPWQASLVLLALSESSILLHKLFSPPTL QAQHDTYHRHLHPGSYSLQWERTPLSIPRTTAFLPFTPTTSTAPPDRSEASLPPAFASTF VPRPPPAASSPGAQPPTTTAAPPTPIEPTQRTHQNSDLALESSTSTEPPPPPIRSPDMTP SAPVLFPEINSPRRFPPQLPATPDLEPAHTPPPLSIPHQDPTDSADPLMGSHLLHHSLPA PPTHPLPSSQLLPAPLTNDPTAIGPVLPFEELHPRRYPENTATFLTRLRSLPSNHLPQPT LNCLLSAVSDQTKVSEEHLWESLQTILPDSQLSNEETNTLGLSTEHLTALAHLYNFQATV YSDRGPILFGPSDTIKRIDITHTTGPPSHFSPGKRLLGSQPSAKGHPSDPLIRAMKSFKV SGNYLPFSEAHNHPTSISHAKNLISNMKNGFDGVLSLLDVSTGQRTGPTPKERIIQIDHY LDTNPGKTTPVVHFAGFAGCGKTYPIQQLLKTKLFKDFRVSCPTTELRTEWKTAMELHGS QSWRFNTWESSILKSSRILVIDEIYKMPRGYLDLSILADPALELVIILGDPLQGEYHSQS KDSSNHRLPSETLRLLPYIDMYCWWSYRIPQCIARLFQIHSFNAWQGVIGSVSTPHDQSP VLTNSHASSLTFNSLGYRSCTISSSQGLTFCDPAIIVLDNYTKWLSSANGLVALTRSRSG VQFMGPSSYVGGTNGSSAMFSDAFNNSLIIMDRYFPSLFPQLKLITSPLTTRGPKLNGAT PSASPTHRSPNFHLPPHIPLSYDRDFVTVNPTLPDQGPETRLDTHFLPPSRLPLHFDLPP AITPPPVSTSVDPPQAKASPVYPGEFFDSLAAFFLPAHDPSTREILHKDQSSNQFPWFDR PFSLSCQPSSLISAKHAPNHDPTLLPASINKRLRFRPSDSPHQITADDVVLGLQLFHSLC RAYSRQPNSTVPFNPELFAECISLNEYAQLSSKTQSTIVANASRSDPDWRHTTVKIFAKA QHKVNDGSIFGSWKACQTLALMHDYVILVLGPVKKYQRIFDNADRPPNIYSHCGKTPNQL RDWCQEHLTHSTPKIANDYTAFDQSQHGESVVLEALKMKRLNIPSHLIQLHVHLKTNVST QFGPLTCMRLTGEPGTYDDNTDYNLAVIYSQYDVGSCPIMVSGDDSLIDHPLPTRHDWPS VLKRLHLRFKLELTSHPLFCGYYVGPAGCIRNPLALFCKLMIAVDDDALDDRRLSYLTEF TTGHLLGESLWHLLPETHVQYQSACFDFFCRRCPRHEKMLLDDSTPALSLLERITSSPRW LTKNAMYLLPAKLRLAITSLSQTQSFPESIEVSHAESELLHYVQ

Options »

Help

For a sequence similarity search, enter:
• a protein or nucleotide sequence
• a UniProt identifier, e.g.
P00750 or A4_HUMAN or UPI0000000001
More...

Protein names	Recommended name: RNA replicase polyprotein Alternative name(s): 206 kDa polyprotein Cleaved into the following 3 chains: Methyltransferase/Protease EC=2.1.1.- EC=3.4.22.- Alternative name(s): 98 kDa protein MET/PRO Putative helicase EC=3.6.4.- Alternative name(s): 42 kDa protein HEL RNA-directed RNA polymerase EC=2.7.7.48 Alternative name(s): 66 kDa protein POL
Organism	Turnip yellow mosaic virus [Reference proteome]
Taxonomic identifier	12154 [NCBI]
Taxonomic lineage	Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Tymovirales › Tymoviridae › Tymovirus
Virus host	Brassica [TaxID: 3705] Cardamine lilacina [TaxID: 82359]

Sequence length	1844 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

Function	Acts as a cysteine protease, methyltransferase and deubiquitinase. The cyteine protease activity cleaves the polyprotein giving rise to mature proteins. The methyltransferase domain is probably involved in viral RNA capping. The deubiquitylating activity counteracts the degradation of the viral polymerase mediated by the host ubiquitin-proteasome system. The polymerase is thus stabilized and infectivity is increased. Ref.4 RNA-directed RNA polymerase is responsible for the replication and transcription of the genome. Ref.4
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Methyltransferase/Protease: Host chloroplast envelope Ref.2 Ref.3. Putative helicase: Host chloroplast envelope Ref.2 Ref.3. RNA-directed RNA polymerase: Host chloroplast envelope Ref.2 Ref.3.
Domain	The OTU-like region is responsible for the deubiquitination activity of this protein.
Sequence similarities	Belongs to the tymovirus NS35 RNA replicase polyprotein family. Contains 1 (+)RNA virus helicase ATP-binding domain. Contains 1 (+)RNA virus helicase C-terminal domain. Contains 1 RdRp catalytic domain.

Keywords
Biological process	Host-virus interaction Modulation of host ubiquitin pathway by viral deubiquitinase Modulation of host ubiquitin pathway by virus Viral RNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Methyltransferase Nucleotidyltransferase Protease RNA-directed RNA polymerase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA processing Inferred from electronic annotation. Source: InterPro modulation by virus of host protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA methyltransferase activity Inferred from electronic annotation. Source: InterPro peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

[1]	"Overlapping open reading frames revealed by complete nucleotide sequencing of turnip yellow mosaic virus genomic RNA." Morch M.D., Boyer J.C., Haenni A.L. Nucleic Acids Res. 16:6157-6173(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Detection and subcellular localization of the turnip yellow mosaic virus 66K replication protein in infected cells." Prod'homme D., Le Panse S., Drugeon G., Jupin I. Virology 281:88-101(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[3]	"Proteolytic processing of turnip yellow mosaic virus replication proteins and functional impact on infectivity." Jakubiec A., Drugeon G., Camborde L., Jupin I. J. Virol. 81:11402-11412(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN, SUBCELLULAR LOCATION.
[4]	"A viral deubiquitylating enzyme targets viral RNA-dependent RNA polymerase and affects viral infectivity." Chenon M., Camborde L., Cheminant S., Jupin I. EMBO J. 31:741-753(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF METHYLTRANSFERASE/PROTEASE.
+	Additional computationally mapped references.

Preferred order of sections

Names and origin

Cleaved into the following 3 chains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Family and domain databases

Entry information

Relevant documents