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P10349 (PLSB_CUCMO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycerol-3-phosphate acyltransferase, chloroplastic

Short name=GPAT
EC=2.3.1.15
OrganismCucurbita moschata (Winter crookneck squash) (Cucurbita pepo var. moschata)
Taxonomic identifier3662 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeCucurbiteaeCucurbita

Protein attributes

Sequence length396 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate. The enzyme from chilling-resistant plants discriminates against non-fluid palmitic acid and selects oleic acid whereas the enzyme from sensitive plants accepts both fatty acids. Squash is chilling-sensitive.

Catalytic activity

Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.

Pathway

Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.

Subcellular location

Plastidchloroplast stroma.

Domain

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.

Sequence similarities

Belongs to the GPAT/DAPAT family.

Caution

May represent a 5'-truncated sequence in which part of the leader sequence is missing.

Ontologies

Keywords
   Biological processLipid biosynthesis
Lipid metabolism
Phospholipid biosynthesis
Phospholipid metabolism
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   Molecular functionAcyltransferase
Transferase
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological_processCDP-diacylglycerol biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentchloroplast stroma

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionglycerol-3-phosphate O-acyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2828Chloroplast
Chain29 – 396368Glycerol-3-phosphate acyltransferase, chloroplastic
PRO_0000024696

Regions

Motif167 – 1726HXXXXD motif

Secondary structure

................................................................... 396
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P10349 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: CB96E8C057B88112

FASTA39643,839
        10         20         30         40         50         60 
MAELIQDKES AQSAATAAAA SSGYERRNEP AHSRKFLDVR SEEELLSCIK KETEAGKLPP 

        70         80         90        100        110        120 
NVAAGMEELY QNYRNAVIES GNPKADEIVL SNMTVALDRI LLDVEDPFVF SSHHKAIREP 

       130        140        150        160        170        180 
FDYYIFGQNY IRPLIDFGNS FVGNLSLFKD IEEKLQQGHN VVLISNHQTE ADPAIISLLL 

       190        200        210        220        230        240 
EKTNPYIAEN TIFVAGDRVL ADPLCKPFSI GRNLICVYSK KHMFDIPELT ETKRKANTRS 

       250        260        270        280        290        300 
LKEMALLLRG GSQLIWIAPS GGRDRPDPST GEWYPAPFDA SSVDNMRRLI QHSDVPGHLF 

       310        320        330        340        350        360 
PLALLCHDIM PPPSQVEIEI GEKRVIAFNG AGLSVAPEIS FEEIAATHKN PEEVREAYSK 

       370        380        390 
ALFDSVAMQY NVLKTAISGK QGLGASTADV SLSQPW 

« Hide

References

[1]"Cloning and nucleotide sequence of cDNA for the plastid glycerol-3-phosphate acyltransferase from squash."
Ishizaki O., Nishida I., Agata K., Eguchi G., Murata N.
FEBS Lett. 238:424-430(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: cv. Shirakikuza.
Tissue: Cotyledon.
[2]"Properties of the plastidial acyl-(acyl-carrier-protein): glycerol-3-phosphate acyltransferase from the chilling-sensitive plant squash (Cucurbita moschata)."
Frentzen M., Nishida I., Murata N.
Plant Cell Physiol. 28:1195-1201(1987)
Cited for: CHARACTERIZATION.
[3]"Analysis of the structure, substrate specificity, and mechanism of squash glycerol-3-phosphate (1)-acyltransferase."
Turnbull A.P., Rafferty J.B., Sedelnikova S.E., Slabas A.R., Schierer T.P., Kroon J.T.M., Simon J.W., Fawcett T., Nishida I., Murata N., Rice D.W.
Structure 9:347-353(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 32-396.
[4]"Substrate recognition and selectivity of plant glycerol-3-phosphate acyltransferases (GPATs) from Cucurbita moscata and Spinacea oleracea."
Tamada T., Feese M.D., Ferri S.R., Kato Y., Yajima R., Toguri T., Kuroki R.
Acta Crystallogr. D 60:13-21(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS) OF 32-396.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y00771 mRNA. Translation: CAA68740.1.
PIRS01660.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1IUQX-ray1.55A32-396[»]
1K30X-ray1.90A32-396[»]
ProteinModelPortalP10349.
SMRP10349. Positions 31-396.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP10349.
UniPathwayUPA00557; UER00612.

Family and domain databases

Gene3D1.10.1200.50. 1 hit.
InterProIPR016222. G3P_O-acylTrfase_chlp.
IPR023083. G3P_O-acylTrfase_N.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamPF01553. Acyltransferase. 1 hit.
PF14829. GPAT_N. 1 hit.
[Graphical view]
PIRSFPIRSF000431. Glycerol-3-P_O-acyltransfrase. 1 hit.
SMARTSM00563. PlsC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP10349.

Entry information

Entry namePLSB_CUCMO
AccessionPrimary (citable) accession number: P10349
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways