Reviewed, UniProtKB/Swiss-Prot P10341 (PHEA_CORGL)

Names and origin

Protein names

Recommended name:
    Prephenate dehydratase
      Short name=PDT
    EC=4.2.1.51

Gene names

Name:	pheA
Ordered Locus Names:	Cgl2899, cg3207

Organism

Corynebacterium glutamicum (Brevibacterium flavum) [Complete proteome] [HAMAP]

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Catalytic activity	Prephenate = phenylpyruvate + H2O + CO2.
Pathway	Amino-acid biosynthesis; L-phenylalanine biosynthesis; phenylpyruvate from prephenate: step 1/1.
Sequence similarities	Contains 1 ACT domain. Contains 1 prephenate dehydratase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Phenylalanine biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro prephenate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Prephenate dehydratase		PRO_0000119177
Regions
	Domain	7 – 190	184	Prephenate dehydratase
	Domain	203 – 280	78	ACT
Sites
	Site	183	1	Essential for activity By similarity
Experimental info
	Sequence conflict	99	1	M → S in AAA23304. Ref.1
	Sequence conflict	224	1	A → G in AAA23304. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P10341-1 [UniParc]. Last modified July 11, 2002. Version 2. Checksum: 8D7F0B1F06851112	FASTA	315	33,740
	10 20 30 40 50 60 MSDAPTVVAY LGPAGTFTEE ALYKFADAGV FGDGEIEQLP AKSPQEAVDA VRHGTAQFAV 70 80 90 100 110 120 VAIENFVDGP VTPTFDALDQ GSNVQIIAEE ELDIAFSIMV RPGTSLADVK TLATHPVGYQ 130 140 150 160 170 180 QVKNWMATTI PDAMYLSASS NGAGAQMVAE GTADAAAAPS RAAELFGLER LVDDVADVRG 190 200 210 220 230 240 ARTRFVAVQA QAAVSEPTGH DRTSVIFSLP NVPGSLVRAL NEFAIRGVDL TRIESRPTRK 250 260 270 280 290 300 VFGTYRFHLD ISGHIRDIPV AEALRALHLQ AEELVFVGSW PSNRAEDSTP QTDQLAKLHK 310 ADEWVRAASE GRKLN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and nucleotide sequence of the Corynebacterium glutamicum pheA gene." Follettie M.T., Sinskey A.J. J. Bacteriol. 167:695-702(1986) [PubMed: 3525519] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M13774 Genomic DNA. Translation: AAA23304.1. BA000036 Genomic DNA. Translation: BAC00293.1. BX927156 Genomic DNA. Translation: CAF20922.1.
PIR	A26044.
RefSeq	NP_602088.1. YP_227138.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1020842. 3343256.
GenomeReviews	Gene locus Cgl2899 in contig BA000036_GR. Gene locus cg3207 in contig BX927147_GR.
KEGG	cgb:cg3207. cgl:NCgl2799.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P10341.
OMA	GLGNYFF.
Enzyme and pathway databases
BioCyc	CGLU196627-1:CG3207-MON.
BRENDA	4.2.1.51. 812.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR001086. Preph_deHydtase. IPR018528. Preph_deHydtase_CS. [Graphical view]
Pfam	PF01842. ACT. 1 hit. PF00800. PDT. 1 hit. [Graphical view]
PROSITE	PS00857. PREPHENATE_DEHYDR_1. 1 hit. PS00858. PREPHENATE_DEHYDR_2. 1 hit. PS51171. PREPHENATE_DEHYDR_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHEA_CORGL

Accession

Primary (citable) accession number: P10341

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 11, 2002
Last modified:	November 3, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)