P10335 (LIP2_STAAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipase 2
EC=3.1.1.3

Alternative name(s):
Glycerol ester hydrolase 2

Gene names

Name:	lip2
Synonyms:	geh

Organism

Staphylococcus aureus

Taxonomic identifier

1280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	690 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Secreted.
Miscellaneous	The expression of Staphylococcus lipase is negatively regulated by bacteriophage lysogenization (lipase conversion).
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 37	37	Potential
	Propeptide	38 – 295	258			PRO_0000017752
	Chain	296 – 690	395	Lipase 2		PRO_0000017753
Regions
	Region	311 – 690	380	Hydrophobic
Sites
	Active site	412	1	Charge relay system By similarity
	Active site	645	1	Charge relay system By similarity

Sequences

	Sequence		Length	Mass (Da)
	P10335 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 8E8E3654D0E01A3B	FASTA	690	76,388
	10 20 30 40 50 60 MLRGQEERKY SIRKYSIGVV SVLAATMFVV SSHEAQASEK TSTNAAAQKE TLNQPGEQGN 70 80 90 100 110 120 AITSHQMQSG KQLDDMHKEN GKSGTVTEGK DTLQSSKHQS TQNSKTIRTQ NDNQVKQDSE 130 140 150 160 170 180 RQGSKQSHQN NATNNTERQN DQVQNTHHAE RNGSQSTTSQ SNDVDKSQPS IPAQKVIPNH 190 200 210 220 230 240 DKAAPTSTTP PSNDKTAPKS TKAQDATTDK HPNQQDTHQP AHQIIDAKQD DTVRQSEQKP 250 260 270 280 290 300 QVGDLSKHID GQNSPEKPTD KNTDNKQLIK DALQAPKTRS TTNAAADAKK VRPLKANQVQ 310 320 330 340 350 360 PLNKYPVVFV HGFLGLVGDN APALYPNYWG GNKFKVIEEL RKQGYNVHQA SVSAFGSNYD 370 380 390 400 410 420 RAVELYYYIK GGRVDYGAAH AAKYGHERYG KTYKGIMPNW EPGKKVHLVG HSMGGQTIRL 430 440 450 460 470 480 MEEFLRNGNK EEIAYHKAHG GEISPLFTGG HNNMVASITT LATPHNGSQA ADKFGNTEAV 490 500 510 520 530 540 RKIMFALNRF MGNKYSNIDL GLTQWGFKQL PNESYIDYIK RVSKSKIWTS DDNAAYDLTL 550 560 570 580 590 600 DGSAKLNNMT SMNPNITYTT YTGVSSHTGP LGYENPDLGT FFLMATTSRI IGHDAREEWR 610 620 630 640 650 660 KNDGVVPVIS SLHPSNQPFV NVTNDEPATR RGIWQVKPII QGWDHVDFIG VDFLDFKRKG 670 680 690 AELANFYTGI INDLLRVEAT ESKGTQLKAS

References

[1]	"Lysogenic conversion of staphylococcal lipase is caused by insertion of the bacteriophage L54a genome into the lipase structural gene." Lee C.Y., Iandolo J.J. J. Bacteriol. 166:385-391(1986) [PubMed: 3009394] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"In vivo processing of Staphylococcus aureus lipase." Rollof J., Normark S. J. Bacteriol. 174:1844-1847(1992) [PubMed: 1548232] [Abstract] Cited for: PROTEIN SEQUENCE OF 296-307, PROTEOLYTIC PROCESSING. Strain: TEN 5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M12715 Genomic DNA. Translation: AAA26633.1.
PIR	A24545.
3D structure databases
ProteinModelPortal	P10335.
SMR	P10335. Positions 295-682.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR005877. Gpos_YSIRK. [Graphical view]
Pfam	PF04650. YSIRK_signal. 1 hit. [Graphical view]
TIGRFAMs	TIGR01168. YSIRK_signal. 1 hit.
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIP2_STAAU

Accession

Primary (citable) accession number: P10335

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 31, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program