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Protein

Acrosin

Gene

ACR

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Enzyme regulationi

Inhibited by SERPINA5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei88Charge relay systemBy similarity1
Active sitei142Charge relay systemBy similarity1
Active sitei240Charge relay systemBy similarity1

GO - Molecular functioni

  • amidase activity Source: UniProtKB
  • copper ion binding Source: UniProtKB
  • DNA binding Source: UniProtKB
  • drug binding Source: UniProtKB
  • fucose binding Source: UniProtKB
  • mannose binding Source: UniProtKB
  • protease binding Source: Reactome
  • serine-type endopeptidase activity Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • acrosome matrix dispersal Source: UniProtKB
  • acrosome reaction Source: UniProtKB
  • activation of adenylate cyclase activity Source: UniProtKB
  • binding of sperm to zona pellucida Source: Ensembl
  • response to steroid hormone Source: Ensembl
  • single fertilization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciZFISH:HS02039-MONOMER.
BRENDAi3.4.21.10. 2681.
ReactomeiR-HSA-1300645. Acrosome Reaction.

Protein family/group databases

MEROPSiS01.223.

Names & Taxonomyi

Protein namesi
Recommended name:
Acrosin (EC:3.4.21.10)
Cleaved into the following 2 chains:
Gene namesi
Name:ACR
Synonyms:ACRS
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:126. ACR.

Subcellular locationi

GO - Cellular componenti

  • acrosomal matrix Source: HGNC
  • extracellular region Source: Reactome
  • Golgi-associated vesicle Source: Ensembl
  • nucleus Source: UniProtKB
  • protein complex Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi49.
OpenTargetsiENSG00000100312.
ENSG00000283539.
PharmGKBiPA24451.

Chemistry databases

ChEMBLiCHEMBL2738.
GuidetoPHARMACOLOGYi2327.

Polymorphism and mutation databases

BioMutaiACR.
DMDMi115502349.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Add BLAST19
ChainiPRO_000002751820 – 421AcrosinAdd BLAST402
ChainiPRO_000002751920 – 42Acrosin light chainAdd BLAST23
ChainiPRO_000002752043 – 343Acrosin heavy chainAdd BLAST301
PropeptideiPRO_0000027521344 – 421Pro-richCuratedAdd BLAST78

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi22N-linked (GlcNAc...)By similarity1
Disulfide bondi25 ↔ 154Interchain (between light and heavy chains)PROSITE-ProRule annotation
Disulfide bondi29 ↔ 162Interchain (between light and heavy chains)PROSITE-ProRule annotation
Disulfide bondi73 ↔ 89PROSITE-ProRule annotation
Disulfide bondi177 ↔ 246PROSITE-ProRule annotation
Disulfide bondi209 ↔ 225PROSITE-ProRule annotation
Glycosylationi210N-linked (GlcNAc...)By similarity1
Disulfide bondi236 ↔ 266PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP10323.
PeptideAtlasiP10323.
PRIDEiP10323.

Expressioni

Gene expression databases

BgeeiENSG00000100312.
CleanExiHS_ACR.
ExpressionAtlasiP10323. baseline and differential.
GenevisibleiP10323. HS.

Organism-specific databases

HPAiHPA048687.

Interactioni

Subunit structurei

Heavy chain (catalytic) and a light chain linked by two disulfide bonds. Forms a heterodimer with SERPINA5.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi106565. 2 interactors.
STRINGi9606.ENSP00000216139.

Chemistry databases

BindingDBiP10323.

Structurei

3D structure databases

ProteinModelPortaliP10323.
SMRiP10323.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 290Peptidase S1PROSITE-ProRule annotationAdd BLAST248

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00860000133744.
HOGENOMiHOG000033792.
HOVERGENiHBG013304.
InParanoidiP10323.
KOiK01317.
OMAiKKVYDWR.
OrthoDBiEOG091G0AH5.
PhylomeDBiP10323.
TreeFamiTF335943.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR012267. Pept_S1A_acrosin.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PANTHERiPTHR24252. PTHR24252. 1 hit.
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001141. Acrosin. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10323-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEMLPTAIL LVLAVSVVAK DNATCDGPCG LRFRQNPQGG VRIVGGKAAQ
60 70 80 90 100
HGAWPWMVSL QIFTYNSHRY HTCGGSLLNS RWVLTAAHCF VGKNNVHDWR
110 120 130 140 150
LVFGAKEITY GNNKPVKAPL QERYVEKIII HEKYNSATEG NDIALVEITP
160 170 180 190 200
PISCGRFIGP GCLPHFKAGL PRGSQSCWVA GWGYIEEKAP RPSSILMEAR
210 220 230 240 250
VDLIDLDLCN STQWYNGRVQ PTNVCAGYPV GKIDTCQGDS GGPLMCKDSK
260 270 280 290 300
ESAYVVVGIT SWGVGCARAK RPGIYTATWP YLNWIASKIG SNALRMIQSA
310 320 330 340 350
TPPPPTTRPP PIRPPFSHPI SAHLPWYFQP PPRPLPPRPP AAQPRPPPSP
360 370 380 390 400
PPPPPPPASP LPPPPPPPPP TPSSTTKLPQ GLSFAKRLQQ LIEVLKGKTY
410 420
SDGKNHYDME TTELPELTST S
Length:421
Mass (Da):45,847
Last modified:October 3, 2006 - v4
Checksum:i7A2F7ECEC98008FA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti64T → R (PubMed:2114285).Curated1
Sequence conflicti64T → R (PubMed:1628652).Curated1
Sequence conflicti226A → V (PubMed:2298447).Curated1
Sequence conflicti268R → L (PubMed:2114285).Curated1
Sequence conflicti268R → L (PubMed:1628652).Curated1
Sequence conflicti296M → V in CAG30252 (PubMed:15461802).Curated1
Sequence conflicti345R → P (PubMed:2114285).Curated1
Sequence conflicti345R → P (PubMed:2298447).Curated1
Sequence conflicti345R → P (PubMed:1628652).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_011650120L → V.Corresponds to variant rs1064734dbSNPEnsembl.1
Natural variantiVAR_011651166F → L.Corresponds to variant rs1064735dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00970 mRNA. Translation: CAA68784.1.
X54017
, X54018, X54019, X54020 Genomic DNA. Translation: CAA37964.1.
M77378 Genomic DNA. Translation: AAA51572.1.
M77379 Genomic DNA. Translation: AAA51573.1.
M77380 Genomic DNA. Translation: AAA51574.1.
M77381 Genomic DNA. Translation: AAA51575.1.
X66188
, X54018, X54019, X54020 Genomic DNA. Translation: CAA46956.1.
CR456366 mRNA. Translation: CAG30252.1.
CCDSiCCDS14101.1.
PIRiS11674.
RefSeqiNP_001088.2. NM_001097.2.
UniGeneiHs.370870.

Genome annotation databases

EnsembliENST00000216139; ENSP00000216139; ENSG00000100312.
ENST00000636109; ENSP00000490828; ENSG00000283539.
GeneIDi49.
KEGGihsa:49.
UCSCiuc003bnh.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00970 mRNA. Translation: CAA68784.1.
X54017
, X54018, X54019, X54020 Genomic DNA. Translation: CAA37964.1.
M77378 Genomic DNA. Translation: AAA51572.1.
M77379 Genomic DNA. Translation: AAA51573.1.
M77380 Genomic DNA. Translation: AAA51574.1.
M77381 Genomic DNA. Translation: AAA51575.1.
X66188
, X54018, X54019, X54020 Genomic DNA. Translation: CAA46956.1.
CR456366 mRNA. Translation: CAG30252.1.
CCDSiCCDS14101.1.
PIRiS11674.
RefSeqiNP_001088.2. NM_001097.2.
UniGeneiHs.370870.

3D structure databases

ProteinModelPortaliP10323.
SMRiP10323.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106565. 2 interactors.
STRINGi9606.ENSP00000216139.

Chemistry databases

BindingDBiP10323.
ChEMBLiCHEMBL2738.
GuidetoPHARMACOLOGYi2327.

Protein family/group databases

MEROPSiS01.223.

Polymorphism and mutation databases

BioMutaiACR.
DMDMi115502349.

Proteomic databases

PaxDbiP10323.
PeptideAtlasiP10323.
PRIDEiP10323.

Protocols and materials databases

DNASUi49.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000216139; ENSP00000216139; ENSG00000100312.
ENST00000636109; ENSP00000490828; ENSG00000283539.
GeneIDi49.
KEGGihsa:49.
UCSCiuc003bnh.5. human.

Organism-specific databases

CTDi49.
DisGeNETi49.
GeneCardsiACR.
H-InvDBHIX0041187.
HGNCiHGNC:126. ACR.
HPAiHPA048687.
MIMi102480. gene.
neXtProtiNX_P10323.
OpenTargetsiENSG00000100312.
ENSG00000283539.
PharmGKBiPA24451.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00860000133744.
HOGENOMiHOG000033792.
HOVERGENiHBG013304.
InParanoidiP10323.
KOiK01317.
OMAiKKVYDWR.
OrthoDBiEOG091G0AH5.
PhylomeDBiP10323.
TreeFamiTF335943.

Enzyme and pathway databases

BioCyciZFISH:HS02039-MONOMER.
BRENDAi3.4.21.10. 2681.
ReactomeiR-HSA-1300645. Acrosome Reaction.

Miscellaneous databases

GeneWikiiAcrosin.
GenomeRNAii49.
PROiP10323.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100312.
CleanExiHS_ACR.
ExpressionAtlasiP10323. baseline and differential.
GenevisibleiP10323. HS.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR012267. Pept_S1A_acrosin.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PANTHERiPTHR24252. PTHR24252. 1 hit.
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001141. Acrosin. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACRO_HUMAN
AccessioniPrimary (citable) accession number: P10323
Secondary accession number(s): Q6ICK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 3, 2006
Last modified: November 30, 2016
This is version 176 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.