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P10323

- ACRO_HUMAN

UniProt

P10323 - ACRO_HUMAN

Protein

Acrosin

Gene

ACR

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 155 (01 Oct 2014)
      Sequence version 4 (03 Oct 2006)
      Previous versions | rss
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    Functioni

    Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Enzyme regulationi

    Inhibited by SERPINA5.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei88 – 881Charge relay systemBy similarity
    Active sitei142 – 1421Charge relay systemBy similarity
    Active sitei240 – 2401Charge relay systemBy similarity

    GO - Molecular functioni

    1. amidase activity Source: UniProtKB
    2. copper ion binding Source: UniProtKB
    3. DNA binding Source: UniProtKB
    4. drug binding Source: UniProtKB
    5. fucose binding Source: UniProtKB
    6. mannose binding Source: UniProtKB
    7. protein binding Source: UniProtKB
    8. serine-type endopeptidase activity Source: UniProtKB
    9. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. acrosome matrix dispersal Source: UniProtKB
    2. acrosome reaction Source: UniProtKB
    3. activation of adenylate cyclase activity Source: UniProtKB
    4. binding of sperm to zona pellucida Source: Ensembl
    5. multicellular organism reproduction Source: Reactome
    6. response to steroid hormone Source: Ensembl
    7. single fertilization Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Enzyme and pathway databases

    BRENDAi3.4.21.10. 2681.
    ReactomeiREACT_163938. Acrosome Reaction.

    Protein family/group databases

    MEROPSiS01.223.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acrosin (EC:3.4.21.10)
    Cleaved into the following 2 chains:
    Gene namesi
    Name:ACR
    Synonyms:ACRS
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 22

    Organism-specific databases

    HGNCiHGNC:126. ACR.

    Subcellular locationi

    GO - Cellular componenti

    1. acrosomal matrix Source: HGNC
    2. extracellular region Source: Reactome
    3. Golgi-associated vesicle Source: Ensembl
    4. nucleus Source: UniProt
    5. protein complex Source: UniProtKB

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA24451.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Add
    BLAST
    Chaini20 – 421402AcrosinPRO_0000027518Add
    BLAST
    Chaini20 – 4223Acrosin light chainPRO_0000027519Add
    BLAST
    Chaini43 – 343301Acrosin heavy chainPRO_0000027520Add
    BLAST
    Propeptidei344 – 42178Pro-richCuratedPRO_0000027521Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi22 – 221N-linked (GlcNAc...)By similarity
    Disulfide bondi25 ↔ 154Interchain (between light and heavy chains)PROSITE-ProRule annotation
    Disulfide bondi29 ↔ 162Interchain (between light and heavy chains)PROSITE-ProRule annotation
    Disulfide bondi73 ↔ 89PROSITE-ProRule annotation
    Disulfide bondi177 ↔ 246PROSITE-ProRule annotation
    Disulfide bondi209 ↔ 225PROSITE-ProRule annotation
    Glycosylationi210 – 2101N-linked (GlcNAc...)By similarity
    Disulfide bondi236 ↔ 266PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiP10323.
    PRIDEiP10323.

    Expressioni

    Gene expression databases

    ArrayExpressiP10323.
    BgeeiP10323.
    CleanExiHS_ACR.
    GenevestigatoriP10323.

    Organism-specific databases

    HPAiHPA048687.

    Interactioni

    Subunit structurei

    Heavy chain (catalytic) and a light chain linked by two disulfide bonds. Forms a heterodimer with SERPINA5.

    Protein-protein interaction databases

    BioGridi106565. 3 interactions.
    STRINGi9606.ENSP00000216139.

    Structurei

    3D structure databases

    ProteinModelPortaliP10323.
    SMRiP10323. Positions 43-301.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini43 – 290248Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000033792.
    HOVERGENiHBG013304.
    InParanoidiP10323.
    KOiK01317.
    OMAiGSNALHM.
    OrthoDBiEOG75B84T.
    PhylomeDBiP10323.
    TreeFamiTF335943.

    Family and domain databases

    InterProiIPR012267. Pept_S1A_acrosin.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001141. Acrosin. 1 hit.
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P10323-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVEMLPTAIL LVLAVSVVAK DNATCDGPCG LRFRQNPQGG VRIVGGKAAQ    50
    HGAWPWMVSL QIFTYNSHRY HTCGGSLLNS RWVLTAAHCF VGKNNVHDWR 100
    LVFGAKEITY GNNKPVKAPL QERYVEKIII HEKYNSATEG NDIALVEITP 150
    PISCGRFIGP GCLPHFKAGL PRGSQSCWVA GWGYIEEKAP RPSSILMEAR 200
    VDLIDLDLCN STQWYNGRVQ PTNVCAGYPV GKIDTCQGDS GGPLMCKDSK 250
    ESAYVVVGIT SWGVGCARAK RPGIYTATWP YLNWIASKIG SNALRMIQSA 300
    TPPPPTTRPP PIRPPFSHPI SAHLPWYFQP PPRPLPPRPP AAQPRPPPSP 350
    PPPPPPPASP LPPPPPPPPP TPSSTTKLPQ GLSFAKRLQQ LIEVLKGKTY 400
    SDGKNHYDME TTELPELTST S 421
    Length:421
    Mass (Da):45,847
    Last modified:October 3, 2006 - v4
    Checksum:i7A2F7ECEC98008FA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti64 – 641T → R(PubMed:2114285)Curated
    Sequence conflicti64 – 641T → R(PubMed:1628652)Curated
    Sequence conflicti226 – 2261A → V(PubMed:2298447)Curated
    Sequence conflicti268 – 2681R → L(PubMed:2114285)Curated
    Sequence conflicti268 – 2681R → L(PubMed:1628652)Curated
    Sequence conflicti296 – 2961M → V in CAG30252. (PubMed:15461802)Curated
    Sequence conflicti345 – 3451R → P(PubMed:2114285)Curated
    Sequence conflicti345 – 3451R → P(PubMed:2298447)Curated
    Sequence conflicti345 – 3451R → P(PubMed:1628652)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti120 – 1201L → V.
    Corresponds to variant rs1064734 [ dbSNP | Ensembl ].
    VAR_011650
    Natural varianti166 – 1661F → L.
    Corresponds to variant rs1064735 [ dbSNP | Ensembl ].
    VAR_011651

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00970 mRNA. Translation: CAA68784.1.
    X54017
    , X54018, X54019, X54020 Genomic DNA. Translation: CAA37964.1.
    M77378 Genomic DNA. Translation: AAA51572.1.
    M77379 Genomic DNA. Translation: AAA51573.1.
    M77380 Genomic DNA. Translation: AAA51574.1.
    M77381 Genomic DNA. Translation: AAA51575.1.
    X66188
    , X54018, X54019, X54020 Genomic DNA. Translation: CAA46956.1.
    CR456366 mRNA. Translation: CAG30252.1.
    CCDSiCCDS14101.1.
    PIRiS11674.
    RefSeqiNP_001088.2. NM_001097.2.
    UniGeneiHs.370870.

    Genome annotation databases

    EnsembliENST00000216139; ENSP00000216139; ENSG00000100312.
    GeneIDi49.
    KEGGihsa:49.
    UCSCiuc003bnh.4. human.

    Polymorphism databases

    DMDMi115502349.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00970 mRNA. Translation: CAA68784.1 .
    X54017
    , X54018 , X54019 , X54020 Genomic DNA. Translation: CAA37964.1 .
    M77378 Genomic DNA. Translation: AAA51572.1 .
    M77379 Genomic DNA. Translation: AAA51573.1 .
    M77380 Genomic DNA. Translation: AAA51574.1 .
    M77381 Genomic DNA. Translation: AAA51575.1 .
    X66188
    , X54018 , X54019 , X54020 Genomic DNA. Translation: CAA46956.1 .
    CR456366 mRNA. Translation: CAG30252.1 .
    CCDSi CCDS14101.1.
    PIRi S11674.
    RefSeqi NP_001088.2. NM_001097.2.
    UniGenei Hs.370870.

    3D structure databases

    ProteinModelPortali P10323.
    SMRi P10323. Positions 43-301.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 106565. 3 interactions.
    STRINGi 9606.ENSP00000216139.

    Chemistry

    BindingDBi P10323.
    ChEMBLi CHEMBL2738.

    Protein family/group databases

    MEROPSi S01.223.

    Polymorphism databases

    DMDMi 115502349.

    Proteomic databases

    PaxDbi P10323.
    PRIDEi P10323.

    Protocols and materials databases

    DNASUi 49.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000216139 ; ENSP00000216139 ; ENSG00000100312 .
    GeneIDi 49.
    KEGGi hsa:49.
    UCSCi uc003bnh.4. human.

    Organism-specific databases

    CTDi 49.
    GeneCardsi GC22P051176.
    H-InvDB HIX0041187.
    HGNCi HGNC:126. ACR.
    HPAi HPA048687.
    MIMi 102480. gene.
    neXtProti NX_P10323.
    PharmGKBi PA24451.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000033792.
    HOVERGENi HBG013304.
    InParanoidi P10323.
    KOi K01317.
    OMAi GSNALHM.
    OrthoDBi EOG75B84T.
    PhylomeDBi P10323.
    TreeFami TF335943.

    Enzyme and pathway databases

    BRENDAi 3.4.21.10. 2681.
    Reactomei REACT_163938. Acrosome Reaction.

    Miscellaneous databases

    GeneWikii Acrosin.
    GenomeRNAii 49.
    NextBioi 191.
    PROi P10323.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P10323.
    Bgeei P10323.
    CleanExi HS_ACR.
    Genevestigatori P10323.

    Family and domain databases

    InterProi IPR012267. Pept_S1A_acrosin.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001141. Acrosin. 1 hit.
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of human proacrosin deduced from its cDNA sequence."
      Baba T., Watanabe K., Kashiwabara S., Arai Y.
      FEBS Lett. 244:296-300(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Testis.
    2. "Nucleotide sequence and exon-intron organization of the human proacrosin gene."
      Keime S., Adham I.M., Engel W.
      Eur. J. Biochem. 190:195-200(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Leukocyte.
    3. "Molecular cloning of human preproacrosin cDNA."
      Adham I.M., Klemm U., Maier W.-M., Engel W.
      Hum. Genet. 84:125-128(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "Molecular cloning, sequencing and restriction mapping of the genomic sequence encoding human proacrosin."
      Vazquez-Levin M.H., Reventos J., Gordon J.W.
      Eur. J. Biochem. 207:23-26(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. Erratum
      Adham I.M., Spitzer U., Schloesser M., Kremling H., Keime S., Engel W.
      Eur. J. Biochem. 207:27-28(1992)
      Cited for: DISCUSSION OF PUBMED:1628652.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    7. "Inhibition of acrosin by protein C inhibitor and localization of protein C inhibitor to spermatozoa."
      Zheng X., Geiger M., Ecke S., Bielek E., Donner P., Eberspacher U., Schleuning W.D., Binder B.R.
      Am. J. Physiol. 267:C466-C472(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION, HETERODIMER WITH SERPINA5.
    8. "Evaluation of the proacrosin/acrosin system and its mechanism of activation in human sperm extracts."
      Zahn A., Furlong L.I., Biancotti J.C., Ghiringhelli P.D., Marijn-Briggiler C.I., Vazquez-Levin M.H.
      J. Reprod. Immunol. 54:43-63(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROPEPTIDE, ACTIVATION.

    Entry informationi

    Entry nameiACRO_HUMAN
    AccessioniPrimary (citable) accession number: P10323
    Secondary accession number(s): Q6ICK2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: October 3, 2006
    Last modified: October 1, 2014
    This is version 155 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 22
      Human chromosome 22: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Peptidase families
      Classification of peptidase families and list of entries
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3