Reviewed,
UniProtKB/Swiss-Prot P10323 (ACRO_HUMAN)
Last modified
November 3, 2009.
Version 110.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Acrosin EC=3.4.21.10 Cleaved into the following 2 chains: 1- Recommended name: Acrosin light chain 2- Recommended name: Acrosin heavy chain | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 421 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome. |
| Catalytic activity | Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa. |
| Subunit structure | Heavy chain (catalytic) and a light chain linked by two disulfide bonds. |
| Sequence similarities | Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 19 | 19 | |||||||||
| Chain | 20 – 421 | 402 | Acrosin | PRO_0000027518 | |||||||
| Chain | 20 – 42 | 23 | Acrosin light chain | PRO_0000027519 | |||||||
| Chain | 43 – 343 | 301 | Acrosin heavy chain | PRO_0000027520 | |||||||
| Propeptide | 344 – 421 | 78 | Pro-rich Probable | PRO_0000027521 | |||||||
Regions | |||||||||||
| Domain | 43 – 290 | 248 | Peptidase S1 | ||||||||
Sites | |||||||||||
| Active site | 88 | 1 | Charge relay system By similarity | ||||||||
| Active site | 142 | 1 | Charge relay system By similarity | ||||||||
| Active site | 240 | 1 | Charge relay system By similarity | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 22 | 1 | N-linked (GlcNAc...) By similarity | ||||||||
| Glycosylation | 210 | 1 | N-linked (GlcNAc...) By similarity | ||||||||
| Disulfide bond | 25 ↔ 154 | Interchain (between light and heavy chains) By similarity | |||||||||
| Disulfide bond | 29 ↔ 162 | Interchain (between light and heavy chains) By similarity | |||||||||
| Disulfide bond | 73 ↔ 89 | By similarity | |||||||||
| Disulfide bond | 177 ↔ 246 | By similarity | |||||||||
| Disulfide bond | 209 ↔ 225 | By similarity | |||||||||
| Disulfide bond | 236 ↔ 266 | By similarity | |||||||||
Natural variations | |||||||||||
| Natural variant | 120 | 1 | L → V: dbSNP rs1064734. | VAR_011650 | |||||||
| Natural variant | 166 | 1 | F → L: dbSNP rs1064735. | VAR_011651 | |||||||
Experimental info | |||||||||||
| Sequence conflict | 64 | 1 | T → R Ref.2 | ||||||||
| Sequence conflict | 64 | 1 | T → R Ref.4 | ||||||||
| Sequence conflict | 226 | 1 | A → V Ref.3 | ||||||||
| Sequence conflict | 268 | 1 | R → L Ref.2 | ||||||||
| Sequence conflict | 268 | 1 | R → L Ref.4 | ||||||||
| Sequence conflict | 296 | 1 | M → V in CAG30252. Ref.6 | ||||||||
| Sequence conflict | 345 | 1 | R → P Ref.2 | ||||||||
| Sequence conflict | 345 | 1 | R → P Ref.3 | ||||||||
| Sequence conflict | 345 | 1 | R → P Ref.4 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Primary structure of human proacrosin deduced from its cDNA sequence." Baba T., Watanabe K., Kashiwabara S., Arai Y. FEBS Lett. 244:296-300(1989) [PubMed: 2493394] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis. |
| [2] | "Nucleotide sequence and exon-intron organization of the human proacrosin gene." Keime S., Adham I.M., Engel W. Eur. J. Biochem. 190:195-200(1990) [PubMed: 2114285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Leukocyte. |
| [3] | "Molecular cloning of human preproacrosin cDNA." Adham I.M., Klemm U., Maier W.-M., Engel W. Hum. Genet. 84:125-128(1990) [PubMed: 2298447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [4] | "Molecular cloning, sequencing and restriction mapping of the genomic sequence encoding human proacrosin." Vazquez-Levin M.H., Reventos J., Gordon J.W. Eur. J. Biochem. 207:23-26(1992) [PubMed: 1628652] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [5] | Adham I.M., Spitzer U., Schloesser M., Kremling H., Keime S., Engel W. Eur. J. Biochem. 207:27-28(1992) Cited for: DISCUSSION OF PUBMED:1628652. |
| [6] | "A genome annotation-driven approach to cloning the human ORFeome." Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I. Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004) [PubMed: 15461802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [7] | "Evaluation of the proacrosin/acrosin system and its mechanism of activation in human sperm extracts." Zahn A., Furlong L.I., Biancotti J.C., Ghiringhelli P.D., Marijn-Briggiler C.I., Vazquez-Levin M.H. J. Reprod. Immunol. 54:43-63(2002) [PubMed: 11839395] [Abstract] Cited for: PROPEPTIDE, ACTIVATION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| Y00970 mRNA. Translation: CAA68784.1. X54017 X54020 Genomic DNA. Translation: CAA37964.1. M77378 Genomic DNA. Translation: AAA51572.1. M77379 Genomic DNA. Translation: AAA51573.1. M77380 Genomic DNA. Translation: AAA51574.1. M77381 Genomic DNA. Translation: AAA51575.1. X66188 X54020 Genomic DNA. Translation: CAA46956.1. CR456366 mRNA. Translation: CAG30252.1. | |
| IPI | IPI00289614. |
| PIR | S11674. |
| RefSeq | NP_001088.2. |
| UniGene | Hs.370870 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1FIZ based on UniProtKB P08001. |
| SMR | P10323. Positions 43-301. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P10323. |
Protein family/group databases | |
| MEROPS | S01.223. |
Proteomic databases | |
| PRIDE | P10323. |
Genome annotation databases | |
| Ensembl | ENST00000216139; ENSP00000216139; ENSG00000100312; Homo sapiens. [Genome view] |
| GeneID | 49. |
| KEGG | hsa:49. |
| NMPDR | fig|9606.3.peg.22000. |
| UCSC | uc003bnh.2. human. |
Organism-specific databases | |
| CTD | 49. |
| GeneCards | GC22P049466. |
| HGNC | HGNC:126. ACR. |
| MIM | 102480. gene. |
| PharmGKB | PA24451. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | P10323. |
| HOVERGEN | P10323. |
| OMA | GLRFRQN. |
Enzyme and pathway databases | |
| BRENDA | 3.4.21.10. 247. |
Gene expression databases | |
| Bgee | P10323. |
| CleanEx | HS_ACR. |
| Genevestigator | P10323. |
| GermOnline | ENSG00000100312. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR012267. Pept_S1A_acrosin. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view] |
| PANTHER | PTHR19355:SF158. Pept_S1A_acrosin. 1 hit. |
| Pfam | PF00089. Trypsin. 1 hit. [Graphical view] |
| PIRSF | PIRSF001141. Acrosin. 1 hit. |
| PRINTS | PR00722. CHYMOTRYPSIN. |
| SMART | SM00020. Tryp_SPc. 1 hit. [Graphical view] |
| PROSITE | PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 191. |
| SOURCE | Search... |
Entry information
| Entry name | ACRO_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P10323 Secondary accession number(s): Q6ICK2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 22 Human chromosome 22: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


