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Protein

Acrosin

Gene

ACR

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Enzyme regulationi

Inhibited by SERPINA5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei88Charge relay systemBy similarity1
Active sitei142Charge relay systemBy similarity1
Active sitei240Charge relay systemBy similarity1

GO - Molecular functioni

  • amidase activity Source: UniProtKB
  • copper ion binding Source: UniProtKB
  • DNA binding Source: UniProtKB
  • drug binding Source: UniProtKB
  • fucose binding Source: UniProtKB
  • mannose binding Source: UniProtKB
  • protease binding Source: Reactome
  • serine-type endopeptidase activity Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • acrosome matrix dispersal Source: UniProtKB
  • acrosome reaction Source: UniProtKB
  • activation of adenylate cyclase activity Source: UniProtKB
  • binding of sperm to zona pellucida Source: Ensembl
  • penetration of zona pellucida Source: Ensembl
  • response to steroid hormone Source: Ensembl
  • single fertilization Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.10 2681
ReactomeiR-HSA-1300645 Acrosome Reaction

Protein family/group databases

MEROPSiS01.223

Names & Taxonomyi

Protein namesi
Recommended name:
Acrosin (EC:3.4.21.10)
Cleaved into the following 2 chains:
Gene namesi
Name:ACR
Synonyms:ACRS
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000100312.10
HGNCiHGNC:126 ACR
MIMi102480 gene
neXtProtiNX_P10323

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi49
OpenTargetsiENSG00000100312
PharmGKBiPA24451

Chemistry databases

ChEMBLiCHEMBL2738
GuidetoPHARMACOLOGYi2327

Polymorphism and mutation databases

BioMutaiACR
DMDMi115502349

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Add BLAST19
ChainiPRO_000002751820 – 421AcrosinAdd BLAST402
ChainiPRO_000002751920 – 42Acrosin light chainAdd BLAST23
ChainiPRO_000002752043 – 343Acrosin heavy chainAdd BLAST301
PropeptideiPRO_0000027521344 – 421Pro-richCuratedAdd BLAST78

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi22N-linked (GlcNAc...) asparagineBy similarity1
Disulfide bondi25 ↔ 154Interchain (between light and heavy chains)PROSITE-ProRule annotation
Disulfide bondi29 ↔ 162Interchain (between light and heavy chains)PROSITE-ProRule annotation
Disulfide bondi73 ↔ 89PROSITE-ProRule annotation
Disulfide bondi177 ↔ 246PROSITE-ProRule annotation
Disulfide bondi209 ↔ 225PROSITE-ProRule annotation
Glycosylationi210N-linked (GlcNAc...) asparagineBy similarity1
Disulfide bondi236 ↔ 266PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP10323
PeptideAtlasiP10323
PRIDEiP10323

Expressioni

Gene expression databases

BgeeiENSG00000100312
CleanExiHS_ACR
ExpressionAtlasiP10323 baseline and differential
GenevisibleiP10323 HS

Organism-specific databases

HPAiHPA048687

Interactioni

Subunit structurei

Heavy chain (catalytic) and a light chain linked by two disulfide bonds. Forms a heterodimer with SERPINA5.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi1065652 interactors.
STRINGi9606.ENSP00000216139

Chemistry databases

BindingDBiP10323

Structurei

3D structure databases

ProteinModelPortaliP10323
SMRiP10323
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 290Peptidase S1PROSITE-ProRule annotationAdd BLAST248

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00900000140784
HOGENOMiHOG000033792
HOVERGENiHBG013304
InParanoidiP10323
KOiK01317
OMAiDWRLIFG
OrthoDBiEOG091G0AH5
PhylomeDBiP10323
TreeFamiTF335943

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR012267 Pept_S1A_acrosin
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PANTHERiPTHR24252 PTHR24252, 1 hit
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PIRSFiPIRSF001141 Acrosin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10323-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEMLPTAIL LVLAVSVVAK DNATCDGPCG LRFRQNPQGG VRIVGGKAAQ
60 70 80 90 100
HGAWPWMVSL QIFTYNSHRY HTCGGSLLNS RWVLTAAHCF VGKNNVHDWR
110 120 130 140 150
LVFGAKEITY GNNKPVKAPL QERYVEKIII HEKYNSATEG NDIALVEITP
160 170 180 190 200
PISCGRFIGP GCLPHFKAGL PRGSQSCWVA GWGYIEEKAP RPSSILMEAR
210 220 230 240 250
VDLIDLDLCN STQWYNGRVQ PTNVCAGYPV GKIDTCQGDS GGPLMCKDSK
260 270 280 290 300
ESAYVVVGIT SWGVGCARAK RPGIYTATWP YLNWIASKIG SNALRMIQSA
310 320 330 340 350
TPPPPTTRPP PIRPPFSHPI SAHLPWYFQP PPRPLPPRPP AAQPRPPPSP
360 370 380 390 400
PPPPPPPASP LPPPPPPPPP TPSSTTKLPQ GLSFAKRLQQ LIEVLKGKTY
410 420
SDGKNHYDME TTELPELTST S
Length:421
Mass (Da):45,847
Last modified:October 3, 2006 - v4
Checksum:i7A2F7ECEC98008FA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti64T → R (PubMed:2114285).Curated1
Sequence conflicti64T → R (PubMed:1628652).Curated1
Sequence conflicti226A → V (PubMed:2298447).Curated1
Sequence conflicti268R → L (PubMed:2114285).Curated1
Sequence conflicti268R → L (PubMed:1628652).Curated1
Sequence conflicti296M → V in CAG30252 (PubMed:15461802).Curated1
Sequence conflicti345R → P (PubMed:2114285).Curated1
Sequence conflicti345R → P (PubMed:2298447).Curated1
Sequence conflicti345R → P (PubMed:1628652).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_011650120L → V. Corresponds to variant dbSNP:rs1064734Ensembl.1
Natural variantiVAR_011651166F → L. Corresponds to variant dbSNP:rs1064735Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00970 mRNA Translation: CAA68784.1
X54017
, X54018, X54019, X54020 Genomic DNA Translation: CAA37964.1
M77378 Genomic DNA Translation: AAA51572.1
M77379 Genomic DNA Translation: AAA51573.1
M77380 Genomic DNA Translation: AAA51574.1
M77381 Genomic DNA Translation: AAA51575.1
X66188
, X54018, X54019, X54020 Genomic DNA Translation: CAA46956.1
CR456366 mRNA Translation: CAG30252.1
CCDSiCCDS14101.1
PIRiS11674
RefSeqiNP_001088.2, NM_001097.2
UniGeneiHs.370870

Genome annotation databases

EnsembliENST00000216139; ENSP00000216139; ENSG00000100312
ENST00000636109; ENSP00000490828; ENSG00000283539
GeneIDi49
KEGGihsa:49
UCSCiuc003bnh.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiACRO_HUMAN
AccessioniPrimary (citable) accession number: P10323
Secondary accession number(s): Q6ICK2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 3, 2006
Last modified: April 25, 2018
This is version 186 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome