P10314 (1A32_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HLA class I histocompatibility antigen, A-32 alpha chain

Alternative name(s):
MHC class I antigen A*32

Gene names

Name:	HLA-A
Synonyms:	HLAA

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the presentation of foreign antigens to the immune system.
Subunit structure	Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Post-translational modification	Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system By similarity.
Polymorphism	The following alleles of A-32 are known: A*32:01, A*32:02, A*32:03, A*32:04, A*32:05 and A*32:06. The sequence shown is that of A*32:01.
Sequence similarities	Belongs to the MHC class I family. Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Host-virus interaction Immunity
Cellular component	MHC I Membrane
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	antigen processing and presentation of peptide antigen via MHC class I Inferred from electronic annotation. Source: UniProtKB-KW immune response Inferred from electronic annotation. Source: InterPro interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	MHC class I protein complex Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24
	Chain	25 – 365	341	HLA class I histocompatibility antigen, A-32 alpha chain		PRO_0000018824
Regions
	Topological domain	25 – 308	284	Extracellular Potential
	Transmembrane	309 – 332	24	Helical; Potential
	Topological domain	333 – 365	33	Cytoplasmic Potential
	Domain	209 – 295	87	Ig-like C1-type
	Region	25 – 114	90	Alpha-1
	Region	115 – 206	92	Alpha-2
	Region	207 – 298	92	Alpha-3
	Region	299 – 308	10	Connecting peptide
Amino acid modifications
	Glycosylation	110	1	N-linked (GlcNAc...) By similarity
	Disulfide bond	125 ↔ 188		By similarity
	Disulfide bond	227 ↔ 283		By similarity
Natural variations
	Natural variant	89 – 90	2	RN → GK in allele A*32:05.		VAR_016656
	Natural variant	89	1	R → G. Corresponds to variant rs1059459 [ dbSNP | Ensembl ].		VAR_056283
	Natural variant	101	1	S → N in allele A*32:03.		VAR_016657
	Natural variant	121	1	M → I in allele A*32:04.		VAR_016831
	Natural variant	129	1	P → S in allele A*32:04.		VAR_016832
	Natural variant	133	1	L → F in allele A*32:04.		VAR_016833
	Natural variant	138	1	Q → R in allele A*32:04.		VAR_016834
	Natural variant	151	1	N → K. Corresponds to variant rs1059509 [ dbSNP | Ensembl ].		VAR_056284
	Natural variant	166	1	I → T. Corresponds to variant rs1059516 [ dbSNP | Ensembl ].		VAR_056285
	Natural variant	168	1	Q → K in allele A*32:04.		VAR_016835
	Natural variant	175	1	R → H in allele A*32:02, allele A*32:04 and allele A*32:06.		VAR_004373
	Natural variant	176	1	V → E in allele A*32:04.		VAR_016836
	Natural variant	180	1	L → Q in allele A*32:02.		VAR_004374
	Natural variant	185	1	E → D in allele A*32:04.		VAR_016837

Sequences

	Sequence		Length	Mass (Da)
	P10314 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: BF7AF225329E0319	FASTA	365	41,048
	10 20 30 40 50 60 MAVMAPRTLL LLLLGALALT QTWAGSHSMR YFFTSVSRPG RGEPRFIAVG YVDDTQFVRF 70 80 90 100 110 120 DSDAASQRME PRAPWIEQEG PEYWDQETRN VKAHSQTDRE SLRIALRYYN QSEAGSHTIQ 130 140 150 160 170 180 MMYGCDVGPD GRLLRGYQQD AYDGKDYIAL NEDLRSWTAA DMAAQITQRK WEAARVAEQL 190 200 210 220 230 240 RAYLEGTCVE WLRRYLENGK ETLQRTDAPK THMTHHAVSD HEATLRCWAL SFYPAEITLT 250 260 270 280 290 300 WQRDGEDQTQ DTELVETRPA GDGTFQKWAS VVVPSGQEQR YTCHVQHEGL PKPLTLRWEP 310 320 330 340 350 360 SSQPTIPIVG IIAGLVLFGA MFAGAVVAAV RWRRKSSDRK GGSYSQAASS DSAQGSDMSL TACKV

References

[1]	Domena J.D. Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE (ALLELE A*32:01).
[2]	"The primary structure of HLA-A32 suggests a region involved in formation of the Bw4/Bw6 epitopes." Wan A.M., Ennis P., Parham P., Holmes N. J. Immunol. 137:3671-3674(1986) [PubMed: 2431040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 25-298 (ALLELE A*32:01).
[3]	"Sequencing of a new HLA-A*32 subtype (A*3202)." Zino E., Severini G.M., Mazzi B., Bordignon C., Benazzi E., Fleischhauer K. Immunogenetics 45:76-77(1996) [PubMed: 8881042] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE A*32:02). Tissue: Blood.
[4]	"Seventeen more novel HLA-A locus alleles." Ellis J., Steiner N.K., Kosman C., Henson V., Mitton W., Koester R., Ng J., Hartzman R.J., Hurley C.K. Tissue Antigens 55:369-373(2000) [PubMed: 10852390] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 26-206 (ALLELES A*32:03 AND A*32:04).
[5]	"Confirmatory exon 2 and 3 sequence for HLA-A*3203." Kanaan C., Hepner J., Thornton A. Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 26-206 (ALLELE A*32:03).
[6]	"A new HLA A-32 allele." Bettinotti M.P., Hadzikadic L., Adams S., Marincola F.M. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE (ALLELE A*32:05).
[7]	Jones P.F., Hurley C.K. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 26-206 (ALLELE A*32:06).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U03907 mRNA. Translation: AAA03605.1. X97120 mRNA. Translation: CAA65786.1. AF072762, AF072761 Genomic DNA. Translation: AAC26020.1. AF137078, AF137077 Genomic DNA. Translation: AAD33738.1. AF139892, AF139891 Genomic DNA. Translation: AAD33849.1. AF517562, AF517561 Genomic DNA. Translation: AAM78538.1. AF217560 mRNA. Translation: AAF28733.1. AF226837, AF226836 Genomic DNA. Translation: AAF29554.1.
IPI	IPI00472825.
PIR	HLHU32. A26088.
UniGene	Hs.181244. Hs.713441.
3D structure databases
ProteinModelPortal	P10314.
SMR	P10314. Positions 25-294.
ModBase	Search...
Protein-protein interaction databases
STRING	P10314.
Polymorphism databases
DMDM	1168210.
Proteomic databases
PRIDE	P10314.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000442068; ENSP00000404428; ENSG00000223980. ENST00000454091; ENSP00000410645; ENSG00000223980.
Organism-specific databases
GeneCards	GC06P029921. GC06Po29897.
H-InvDB	HIX0078423.
HGNC	HGNC:4931. HLA-A.
MIM	142800. gene.
neXtProt	NX_P10314.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	HBG016709.
Gene expression databases
CleanEx	HS_HLA-A.
Genevestigator	P10314.
GermOnline	ENSG00000204632. Homo sapiens.
Family and domain databases
InterPro	IPR007110. Ig-like. IPR013783. Ig-like_fold. IPR003006. Ig/MHC_CS. IPR003597. Ig_C1-set. IPR011161. MHC_I-like_Ag-recog. IPR011162. MHC_I/II-like_Ag-recog. IPR001039. MHC_I_a_a1/a2. IPR010579. MHC_I_a_C. [Graphical view]
Gene3D	G3DSA:2.60.40.10. Ig-like_fold. 1 hit. G3DSA:3.30.500.10. MHC_I-like_Ag-recog. 1 hit.
Pfam	PF07654. C1-set. 1 hit. PF00129. MHC_I. 1 hit. PF06623. MHC_I_C. 1 hit. [Graphical view]
PRINTS	PR01638. MHCCLASSI.
SMART	SM00407. IGc1. 1 hit. [Graphical view]
SUPFAM	SSF54452. MHC_I/II-like_Ag-recog. 1 hit.
PROSITE	PS50835. IG_LIKE. 1 hit. PS00290. IG_MHC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

1A32_HUMAN

Accession

Primary (citable) accession number: P10314
Secondary accession number(s): O77937 , Q29838, Q8MHN9, Q9MYE9, Q9MYG5, Q9TQF5, Q9TQF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.