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Protein

Terminase, large subunit gp19

Gene

19

Organism
Enterobacteria phage T3 (Bacteriophage T3)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The terminase large subunit acts as an ATP driven molecular motor necessary for viral DNA translocation into empty capsids and as an endonuclease that cuts the viral genome at a unique and precise dsDNA sequence to initiate and to end a packaging reaction. The terminase lies at a unique vertex of the procapsid and is composed of two subunits, a small terminase subunit involved in viral DNA recognition (packaging sequence), and a large terminase subunit possessing endonucleolytic and ATPase activities. Both terminase subunits heterooligomerize and are docked on the portal protein to form the packaging machine. The terminase large subunit exhibits endonuclease activity and cleaves the viral genome concatemer. Once the DNA is packaged, the terminase detaches from the connector and gets replaced by the tail to finish maturation of the virion (By similarity).By similarity1 Publication

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi364Magnesium 1 and 2; catalytic; for nuclease activityBy similarity1
Metal bindingi420Magnesium 2; catalytic; for nuclease activityBy similarity1
Metal bindingi518Magnesium 1; catalytic; for nuclease activityBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi58 – 65ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processViral genome packaging, Virus exit from host cell
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Terminase, large subunit gp19 (EC:3.1.-.-1 Publication)
Alternative name(s):
DNA maturase B
DNA-packaging protein B
Gene product 19
Short name:
gp19
Gene namesi
Name:19
OrganismiEnterobacteria phage T3 (Bacteriophage T3)
Taxonomic identifieri10759 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeAutographivirinaeT7virus
Virus hostiEscherichia coli [TaxID: 562]

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi61G → D: Defective in DNA packaging. 1 Publication1
Mutagenesisi344H → D: Defective in DNA packaging. No effect on DNA cleavage. 1 Publication1
Mutagenesisi347H → R: Possibly defective in packaging initiation. 1 Publication1
Mutagenesisi369G → D: Defective in DNA cleavage. 1 Publication1
Mutagenesisi424G → E: Defective in DNA cleavage. 2 Publications1
Mutagenesisi430K → T: No effect on DNA packaging. 1 Publication1
Mutagenesisi553H → L: No effect on DNA packaging. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001065401 – 586Terminase, large subunit gp19Add BLAST586

Proteomic databases

PRIDEiP10310.

Interactioni

Subunit structurei

Homopentamer. Interacts with the terminase small subunit gp19; the active complex is probably heterooligomeric. Interacts with the head-to-tail connector protein gp8.By similarity

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 229ATPase activityBy similarityAdd BLAST229
Regioni344 – 429Nuclease activityBy similarityAdd BLAST86
Regioni571 – 586Involved in prohead binding1 PublicationAdd BLAST16

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 65Walker A motifBy similarity8
Motifi156 – 161Walker B motifBy similarity6

Sequence similaritiesi

Belongs to the T7likevirus large terminase family.Curated

Sequencei

Sequence statusi: Complete.

P10310-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTQSNRNAL VVAQLKGDFV AFLFVLWKAL NLPVPTKCQI DMAKVLANGD
60 70 80 90 100
NKKFILQAFR GIGKSFITCA FVVWTLWRDP QLKILIVSAS KERADLNSIF
110 120 130 140 150
IKNIIDLLPF LDELKPSPGQ RDSVISFDVG PAKPDHSPSV KSVGITGQLT
160 170 180 190 200
GSRADIIIAD DVEIPSNSAT QGAREKLWTL VQEFRALLKP LPTSRVIYLG
210 220 230 240 250
TPQTEMTLYK ELEDNRGYTT IIWPALYPRS REEDLYYGER LAPMLREEFN
260 270 280 290 300
DGFEMLQGQP TDPVRFDMED LRERELEYGK AGFTLQFMLN PNLSDAEKYP
310 320 330 340 350
LRLRDAIVCG LDFEKAPMHY QWLPNRQNRN EELPNVGLKG DDIHSYHSCS
360 370 380 390 400
QNTGQYQQRI LVIDPSGRGK DETGYAVLFT LNGYIYLMEA GGFPDGYSDK
410 420 430 440 450
TLESLAKKAN EWKVQTVVFE SNFGDGMFGK VFSPVLLKHH AAALEEIRAR
460 470 480 490 500
GMKELRICDT LEPVLSTHRL VIRDEVIRED YQTARDADGK HDVRYSLFYQ
510 520 530 540 550
LTRMAREKGA VAHDDRLDAF RLGVEFLRST MELDAVKVEA EVLEAFLEEH
560 570 580
MEHPIHSAGH VVTAMVDGME LYWEDDDVNG DRFINW
Length:586
Mass (Da):66,673
Last modified:July 1, 1989 - v1
Checksum:iF9FD00D59DDF2D26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14784 Genomic DNA. Translation: AAA92528.1.
PIRiF23476. JVBPB3.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14784 Genomic DNA. Translation: AAA92528.1.
PIRiF23476. JVBPB3.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP10310.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiTERL_BPT3
AccessioniPrimary (citable) accession number: P10310
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 12, 2017
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.