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Protein

Holin

Gene

17.5

Organism
Enterobacteria phage T3 (Bacteriophage T3)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Accumulates harmlessly in the cytoplasmic membrane until it reaches a critical concentration that triggers the formation of micron-scale pores (holes) causing host cell membrane disruption and endolysin escape into the periplasmic space. Participates in determining the precise timing of host cell lysis. Participates with the endolysin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles from the host cell.UniRule annotation2 Publications

GO - Biological processi

Keywordsi

Biological processCytolysis, Host cell lysis by virus, Virus exit from host cell

Names & Taxonomyi

Protein namesi
Recommended name:
HolinUniRule annotation
Alternative name(s):
Lysis protein
Gene namesi
Name:17.5
Synonyms:lys
OrganismiEnterobacteria phage T3 (Bacteriophage T3)
Taxonomic identifieri10759 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeAutographivirinaeT7virus
Virus hostiEscherichia coli [TaxID: 562]

Subcellular locationi

  • Host cell inner membrane UniRule annotation1 Publication; Single-pass type II membrane protein UniRule annotation1 Publication; Periplasmic side UniRule annotation1 Publication

  • Note: Classified as a class II holin although it seems to have only one transmembrane domain.1 PublicationUniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 36CytoplasmicUniRule annotationAdd BLAST36
Transmembranei37 – 55Helical; Signal-anchor for type II membrane proteinUniRule annotationAdd BLAST19
Topological domaini56 – 67PeriplasmicUniRule annotationAdd BLAST12

GO - Cellular componenti

Keywords - Cellular componenti

Host cell inner membrane, Host cell membrane, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001065321 – 67HolinAdd BLAST67

Interactioni

Subunit structurei

Homomultimer.UniRule annotation1 Publication

Family & Domainsi

Sequence similaritiesi

Belongs to the T7likevirus holin family.UniRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Family and domain databases

HAMAPiMF_04108. HOLIN_T7. 1 hit.
InterProiView protein in InterPro
IPR019682. Phage_T7_Gp17.5_holin.
PfamiView protein in Pfam
PF10746. Phage_holin_2_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P10307-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSLDFNNEL IKAAPIVGTG VADVSARRFF GLSLNEWFYV ARIAYTVVXI
60
GAKVVDKIID WKKATKE
Length:67
Mass (Da):7,472
Last modified:July 1, 1989 - v1
Checksum:iE751BDC9883F4AD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14784 Genomic DNA. Translation: AAA92524.1. Different termination.
PIRiB23476. Q7BPT3.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14784 Genomic DNA. Translation: AAA92524.1. Different termination.
PIRiB23476. Q7BPT3.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

HAMAPiMF_04108. HOLIN_T7. 1 hit.
InterProiView protein in InterPro
IPR019682. Phage_T7_Gp17.5_holin.
PfamiView protein in Pfam
PF10746. Phage_holin_2_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHOLIN_BPT3
AccessioniPrimary (citable) accession number: P10307
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: January 18, 2017
This is version 36 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.