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Protein

Spanin, inner membrane subunit

Gene

18.5

Organism
Enterobacteria phage T3 (Bacteriophage T3)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Component of the spanin complex that disrupts the host outer membrane and participates in cell lysis during virus exit. The spanin complex conducts the final step in host lysis by disrupting the outer membrane after holin and endolysin action have permeabilized the inner membrane and degraded the host peptidoglycans. Host outer membrane disruption is possibly due to local fusion between the inner and outer membrane performed by the spanin complex.By similarity

GO - Biological processi

Keywordsi

Biological processCytolysis, Host cell lysis by virus, Virus exit from host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Spanin, inner membrane subunitBy similarity
Short name:
i-spanin
Alternative name(s):
Gene product 18.5
Short name:
Gp18.5
Gene namesi
Name:18.5
OrganismiEnterobacteria phage T3 (Bacteriophage T3)
Taxonomic identifieri10759 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeAutographivirinaeT7virus
Virus hostiEscherichia coli [TaxID: 562]

Subcellular locationi

  • Host cell inner membrane By similarity; Single-pass type II membrane protein By similarity; Periplasmic side By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicBy similarity7
Transmembranei8 – 24Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini25 – 143PeriplasmicBy similarityAdd BLAST119

GO - Cellular componenti

Keywords - Cellular componenti

Host cell inner membrane, Host cell membrane, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001065361 – 147Spanin, inner membrane subunitAdd BLAST147

Interactioni

Subunit structurei

Interacts (via C-terminus) with the spanin outer lipoprotein subunit (o-spanin) (via C-terminus). Part of the spanin complex which spans the entire periplasmic space. The spanin complex is composed of spanin inner membrane subunit and spanin outer membrane subunit.By similarity

Structurei

3D structure databases

SMRiP10305.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the T7likevirus i-spanin family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Family and domain databases

InterProiView protein in InterPro
IPR004929. i-spanin.
IPR016417. i-spanin_T7likevirus.
PfamiView protein in Pfam
PF03245. Phage_lysis. 1 hit.
PIRSFiPIRSF004485. T7_18-5_prd. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD033200. Phge_lysis-endo-Pept_T7/18-5. 1 hit.

Sequencei

Sequence statusi: Complete.

P10305-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEFLRKLIP WVLAGMLFGL GWHLGSDSMD AKWKQEVHNE YVKRVEATAS
60 70 80 90 100
TQRAINEISA KYQEDLAALE GSTDRIISDL RQDNKRLRVR VKPTGTSEGQ
110 120 130 140
CGFEPDDRAE LDDRDAKRIL SVTQKGDAWI RALQDTIREL QRKQEIK
Length:147
Mass (Da):16,911
Last modified:July 1, 1989 - v1
Checksum:iB6FDD684ADF3FFD8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14784 Genomic DNA. Translation: AAA92526.1.
PIRiD23476. Q8BPT3.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSPAN1_BPT3
AccessioniPrimary (citable) accession number: P10305
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: March 15, 2017
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families