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Protein

Ras-related protein R-Ras

Gene

RRAS

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates the organization of the actin cytoskeleton.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi36 – 449GTP
Nucleotide bindingi83 – 875GTPBy similarity
Nucleotide bindingi142 – 1454GTP
Nucleotide bindingi172 – 1743GTP

GO - Molecular functioni

  • GDP binding Source: UniProtKB
  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB-KW
  • protein complex binding Source: MGI

GO - Biological processi

  • axon guidance Source: Reactome
  • face morphogenesis Source: UniProtKB
  • leukocyte differentiation Source: UniProtKB
  • metabolic process Source: GOC
  • negative regulation of cell migration Source: Ensembl
  • positive regulation of angiogenesis Source: UniProtKB
  • Ras protein signal transduction Source: ProtInc
  • regulation of ERK1 and ERK2 cascade Source: UniProtKB
  • regulation of protein kinase B signaling Source: UniProtKB
  • synaptic transmission Source: Reactome
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_19266. Sema4D mediated inhibition of cell attachment and migration.
REACT_19279. SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion.
REACT_20568. CREB phosphorylation through the activation of Ras.
SignaLinkiP10301.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein R-Ras
Alternative name(s):
p23
Gene namesi
Name:RRAS
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:10447. RRAS.

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity

  • Note: Inner surface of plasma membrane possibly with attachment requiring acylation of the C-terminal cysteine (By similarity with RAS).

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • focal adhesion Source: UniProtKB
  • intracellular Source: GOC
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34861.

Polymorphism and mutation databases

BioMutaiRRAS.
DMDMi133486.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 215215Ras-related protein R-RasPRO_0000082650Add
BLAST
Propeptidei216 – 2183Removed in mature formBy similarityPRO_0000281300

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei215 – 2151Cysteine methyl esterBy similarity
Lipidationi215 – 2151S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiP10301.
PaxDbiP10301.
PeptideAtlasiP10301.
PRIDEiP10301.

2D gel databases

SWISS-2DPAGEP10301.

PTM databases

PhosphoSiteiP10301.

Expressioni

Gene expression databases

BgeeiP10301.
CleanExiHS_RRAS.
ExpressionAtlasiP10301. baseline and differential.
GenevisibleiP10301. HS.

Organism-specific databases

HPAiCAB010160.

Interactioni

Subunit structurei

Interacts with PLCE1. Interacts (active GTP-bound form preferentially) with RGS14 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
NCK1P163333EBI-968703,EBI-389883

Protein-protein interaction databases

BioGridi112151. 21 interactions.
IntActiP10301. 14 interactions.
STRINGi9606.ENSP00000246792.

Structurei

Secondary structure

1
218
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi29 – 368Combined sources
Helixi42 – 5110Combined sources
Beta strandi64 – 729Combined sources
Beta strandi75 – 839Combined sources
Turni87 – 904Combined sources
Helixi94 – 1007Combined sources
Beta strandi102 – 1098Combined sources
Helixi113 – 13018Combined sources
Beta strandi137 – 1426Combined sources
Helixi144 – 1496Combined sources
Helixi154 – 16310Combined sources
Beta strandi167 – 1704Combined sources
Turni173 – 1764Combined sources
Helixi179 – 19315Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FN4X-ray1.65A23-201[»]
ProteinModelPortaliP10301.
SMRiP10301. Positions 24-196.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10301.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 669Effector region

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00780000121849.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiP10301.
KOiK07829.
OMAiRSEASTF.
OrthoDBiEOG7QVM41.
PhylomeDBiP10301.
TreeFamiTF312796.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00173. RAS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10301-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSGAASGTG RGRPRGGGPG PGDPPPSETH KLVVVGGGGV GKSALTIQFI
60 70 80 90 100
QSYFVSDYDP TIEDSYTKIC SVDGIPARLD ILDTAGQEEF GAMREQYMRA
110 120 130 140 150
GHGFLLVFAI NDRQSFNEVG KLFTQILRVK DRDDFPVVLV GNKADLESQR
160 170 180 190 200
QVPRSEASAF GASHHVAYFE ASAKLRLNVD EAFEQLVRAV RKYQEQELPP
210
SPPSAPRKKG GGCPCVLL
Length:218
Mass (Da):23,480
Last modified:July 1, 1989 - v1
Checksum:i437F73170670EB28
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14949, M14948 Genomic DNA. Translation: AAA60256.1.
AF493920 mRNA. Translation: AAM12634.1.
BT006805 mRNA. Translation: AAP35451.1.
CR541944 mRNA. Translation: CAG46742.1.
CR541967 mRNA. Translation: CAG46765.1.
CH471177 Genomic DNA. Translation: EAW52506.1.
BC016286 mRNA. Translation: AAH16286.1.
BC016318 mRNA. Translation: AAH16318.1.
CCDSiCCDS12774.1.
PIRiA26159. TVHURR.
RefSeqiNP_006261.1. NM_006270.3.
UniGeneiHs.515536.

Genome annotation databases

EnsembliENST00000246792; ENSP00000246792; ENSG00000126458.
GeneIDi6237.
KEGGihsa:6237.
UCSCiuc002pop.1. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14949, M14948 Genomic DNA. Translation: AAA60256.1.
AF493920 mRNA. Translation: AAM12634.1.
BT006805 mRNA. Translation: AAP35451.1.
CR541944 mRNA. Translation: CAG46742.1.
CR541967 mRNA. Translation: CAG46765.1.
CH471177 Genomic DNA. Translation: EAW52506.1.
BC016286 mRNA. Translation: AAH16286.1.
BC016318 mRNA. Translation: AAH16318.1.
CCDSiCCDS12774.1.
PIRiA26159. TVHURR.
RefSeqiNP_006261.1. NM_006270.3.
UniGeneiHs.515536.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FN4X-ray1.65A23-201[»]
ProteinModelPortaliP10301.
SMRiP10301. Positions 24-196.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112151. 21 interactions.
IntActiP10301. 14 interactions.
STRINGi9606.ENSP00000246792.

PTM databases

PhosphoSiteiP10301.

Polymorphism and mutation databases

BioMutaiRRAS.
DMDMi133486.

2D gel databases

SWISS-2DPAGEP10301.

Proteomic databases

MaxQBiP10301.
PaxDbiP10301.
PeptideAtlasiP10301.
PRIDEiP10301.

Protocols and materials databases

DNASUi6237.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000246792; ENSP00000246792; ENSG00000126458.
GeneIDi6237.
KEGGihsa:6237.
UCSCiuc002pop.1. human.

Organism-specific databases

CTDi6237.
GeneCardsiGC19M050138.
HGNCiHGNC:10447. RRAS.
HPAiCAB010160.
MIMi165090. gene.
neXtProtiNX_P10301.
PharmGKBiPA34861.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00780000121849.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiP10301.
KOiK07829.
OMAiRSEASTF.
OrthoDBiEOG7QVM41.
PhylomeDBiP10301.
TreeFamiTF312796.

Enzyme and pathway databases

ReactomeiREACT_19266. Sema4D mediated inhibition of cell attachment and migration.
REACT_19279. SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion.
REACT_20568. CREB phosphorylation through the activation of Ras.
SignaLinkiP10301.

Miscellaneous databases

EvolutionaryTraceiP10301.
GeneWikiiRRAS.
GenomeRNAii6237.
NextBioi24215.
PROiP10301.
SOURCEiSearch...

Gene expression databases

BgeeiP10301.
CleanExiHS_RRAS.
ExpressionAtlasiP10301. baseline and differential.
GenevisibleiP10301. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00173. RAS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of the human and murine R-ras genes, novel genes closely related to ras proto-oncogenes."
    Lowe D.G., Capon D.J., Delwart E., Sakaguchi A.Y., Naylor S.L., Goeddel D.V.
    Cell 48:137-146(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Puhl H.L. III, Ikeda S.R., Aronstam R.S.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain and Uterus.
  7. "Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in human adipocytes."
    Aboulaich N., Vainonen J.P., Stralfors P., Vener A.V.
    Biochem. J. 383:237-248(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 177-188.
    Tissue: Adipocyte.
  8. "The small GTPase R-Ras regulates organization of actin and drives membrane protrusions through the activity of PLCepsilon."
    Ada-Nguema A.S., Xenias H., Sheetz M.P., Keely P.J.
    J. Cell Sci. 119:1307-1319(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PLCE1.
  9. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  10. "Germline missense mutations affecting KRAS Isoform B are associated with a severe Noonan syndrome phenotype."
    Carta C., Pantaleoni F., Bocchinfuso G., Stella L., Vasta I., Sarkozy A., Digilio C., Palleschi A., Pizzuti A., Grammatico P., Zampino G., Dallapiccola B., Gelb B.D., Tartaglia M.
    Am. J. Hum. Genet. 79:129-135(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 23-201 IN COMPLEX WITH GDP.

Entry informationi

Entry nameiRRAS_HUMAN
AccessioniPrimary (citable) accession number: P10301
Secondary accession number(s): Q6FH12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: July 22, 2015
This is version 165 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.