P10301 (RRAS_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein R-Ras

Alternative name(s):
p23

Gene names

Name:

RRAS

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulates the organization of the actin cytoskeleton. Ref.8
Subunit structure	Interacts with PLCE1. Interacts (active GTP-bound form preferentially) with RGS14 By similarity. Ref.8
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side By similarity. Note: Inner surface of plasma membrane possibly with attachment requiring acylation of the C-terminal cysteine (By similarity with RAS).
Sequence similarities	Belongs to the small GTPase superfamily. Ras family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Prenylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	Ras protein signal transduction Traceable author statement Ref.1. Source: ProtInc axon guidance Traceable author statement. Source: Reactome negative regulation of cell migration Inferred from electronic annotation. Source: Compara positive regulation of angiogenesis Inferred from mutant phenotype PubMed 21393242. Source: UniProtKB synaptic transmission Traceable author statement. Source: Reactome
Cellular_component	plasma membrane Traceable author statement. Source: Reactome
Molecular_function	GDP binding Inferred from direct assay Ref.9. Source: UniProtKB GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from direct assay Ref.9. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NCK1	P16333	3	EBI-968703,EBI-389883

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 215

215

Ras-related protein R-Ras

PRO_0000082650

Propeptide

216 – 218

3

Removed in mature form By similarity

PRO_0000281300

Regions

Nucleotide binding

36 – 44

9

GTP

Nucleotide binding

83 – 87

5

GTP By similarity

Nucleotide binding

142 – 145

4

GTP

Nucleotide binding

172 – 174

3

GTP

Motif

58 – 66

9

Effector region By similarity

Amino acid modifications

Modified residue

215

1

Cysteine methyl ester By similarity

Lipidation

215

1

S-geranylgeranyl cysteine By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

218

Helix Strand Turn

Details...

Beta strand

29 – 36

8

Helix

42 – 51

10

Beta strand

64 – 72

9

Beta strand

75 – 83

9

Turn

87 – 90

4

Helix

94 – 100

7

Beta strand

102 – 109

8

Helix

113 – 130

18

Beta strand

137 – 142

6

Helix

144 – 149

6

Helix

154 – 163

10

Beta strand

167 – 170

4

Turn

173 – 176

4

Helix

179 – 193

15

Sequences

	Sequence		Length	Mass (Da)
	P10301 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 437F73170670EB28	FASTA	218	23,480
	10 20 30 40 50 60 MSSGAASGTG RGRPRGGGPG PGDPPPSETH KLVVVGGGGV GKSALTIQFI QSYFVSDYDP 70 80 90 100 110 120 TIEDSYTKIC SVDGIPARLD ILDTAGQEEF GAMREQYMRA GHGFLLVFAI NDRQSFNEVG 130 140 150 160 170 180 KLFTQILRVK DRDDFPVVLV GNKADLESQR QVPRSEASAF GASHHVAYFE ASAKLRLNVD 190 200 210 EAFEQLVRAV RKYQEQELPP SPPSAPRKKG GGCPCVLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the human and murine R-ras genes, novel genes closely related to ras proto-oncogenes." Lowe D.G., Capon D.J., Delwart E., Sakaguchi A.Y., Naylor S.L., Goeddel D.V. Cell 48:137-146(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Uterus.
[7]	"Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in human adipocytes." Aboulaich N., Vainonen J.P., Stralfors P., Vener A.V. Biochem. J. 383:237-248(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 177-188. Tissue: Adipocyte.
[8]	"The small GTPase R-Ras regulates organization of actin and drives membrane protrusions through the activity of PLCepsilon." Ada-Nguema A.S., Xenias H., Sheetz M.P., Keely P.J. J. Cell Sci. 119:1307-1319(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PLCE1.
[9]	"Germline missense mutations affecting KRAS Isoform B are associated with a severe Noonan syndrome phenotype." Carta C., Pantaleoni F., Bocchinfuso G., Stella L., Vasta I., Sarkozy A., Digilio C., Palleschi A., Pizzuti A., Grammatico P., Zampino G., Dallapiccola B., Gelb B.D., Tartaglia M. Am. J. Hum. Genet. 79:129-135(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 23-201 IN COMPLEX WITH GDP.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M14949, M14948 Genomic DNA. Translation: AAA60256.1.
AF493920 mRNA. Translation: AAM12634.1.
BT006805 mRNA. Translation: AAP35451.1.
CR541944 mRNA. Translation: CAG46742.1.
CR541967 mRNA. Translation: CAG46765.1.
CH471177 Genomic DNA. Translation: EAW52506.1.
BC016286 mRNA. Translation: AAH16286.1.
BC016318 mRNA. Translation: AAH16318.1.

IPI

IPI00020418.

PIR

TVHURR. A26159.

RefSeq

NP_006261.1. NM_006270.3.

UniGene

Hs.515536.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FN4	X-ray	1.65	A	23-201	[»]

ProteinModelPortal

P10301.

ModBase

Search...

Protein-protein interaction databases

IntAct

P10301. 14 interactions.

STRING

9606.ENSP00000246792.

PTM databases

PhosphoSite

P10301.

Polymorphism databases

DMDM

133486.

2D gel databases

SWISS-2DPAGE

P10301.

Proteomic databases

PaxDb

P10301.

PeptideAtlas

P10301.

PRIDE

P10301.

Protocols and materials databases

DNASU

6237.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000246792; ENSP00000246792; ENSG00000126458.

GeneID

6237.

KEGG

hsa:6237.

UCSC

uc002pop.1. human.

Organism-specific databases

CTD

6237.

GeneCards

GC19M050138.

HGNC

HGNC:10447. RRAS.

HPA

CAB010160.

MIM

165090. gene.

neXtProt

NX_P10301.

PharmGKB

PA34861.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG1100.

HOGENOM

HOG000233973.

HOVERGEN

HBG009351.

InParanoid

P10301.

KO

K07829.

OMA

FGASHHV.

OrthoDB

EOG4P5KB6.

PhylomeDB

P10301.

Enzyme and pathway databases

Pathway_Interaction_DB

ephbfwdpathway. EPHB forward signaling.

Reactome

REACT_111045. Developmental Biology.
REACT_13685. Neuronal System.

Gene expression databases

Bgee

P10301.

CleanEx

HS_RRAS.

Genevestigator

P10301.

GermOnline

ENSG00000126458. Homo sapiens.

Family and domain databases

InterPro

IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]

PANTHER

PTHR24070. PTHR24070. 1 hit.

Pfam

PF00071. Ras. 1 hit.
[Graphical view]

PRINTS

PR00449. RASTRNSFRMNG.

SMART

SM00173. RAS. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

PROSITE

PS51421. RAS. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P10301.

GenomeRNAi

6237.

NextBio

24215.

SOURCE

Search...

Entry information

Entry name

RRAS_HUMAN

Accession

Primary (citable) accession number: P10301
Secondary accession number(s): Q6FH12

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 1, 2013
This is version 141 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.