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Protein

T-cell surface glycoprotein CD8 beta chain

Gene

Cd8b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched. Once LCK recruited, it initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). Additionally, plays a critical role in thymic selection of CD8+ T-cells.By similarity3 Publications

GO - Biological processi

Keywordsi

Biological processAdaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiR-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD8 beta chain
Alternative name(s):
Lymphocyte antigen 3
T-cell membrane glycoprotein Ly-3
T-cell surface glycoprotein Lyt-3
CD_antigen: CD8b
Gene namesi
Name:Cd8b
Synonyms:Cd8b1, Ly-3, Lyt-3, Lyt3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:88347 Cd8b1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 175ExtracellularSequence analysisAdd BLAST154
Transmembranei176 – 196HelicalSequence analysisAdd BLAST21
Topological domaini197 – 213CytoplasmicSequence analysisAdd BLAST17

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

The lack of Cd8b reduces but does not completely abolish thymic maturation of CD8+ T-cells. However, Cd8-depleted mice mount normal primary cytotoxic CD8 responses upon acute viral infections.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000001464422 – 213T-cell surface glycoprotein CD8 beta chainAdd BLAST192

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi34N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi41 ↔ 117PROSITE-ProRule annotation2 Publications

Post-translational modificationi

Palmitoylated at the cytoplasmic tail and thereby targets the heterodimer CD8A/CD8B to lipid rafts unlike CD8A homodimers.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP10300
PaxDbiP10300
PRIDEiP10300

PTM databases

iPTMnetiP10300
PhosphoSitePlusiP10300
SwissPalmiP10300

Expressioni

Gene expression databases

BgeeiENSMUSG00000053044
CleanExiMM_CD8B1
ExpressionAtlasiP10300 baseline and differential
GenevisibleiP10300 MM

Interactioni

Subunit structurei

Forms disulfide-linked heterodimers with CD8A at the cell surface. Interacts with CD3D; this interaction couples TCR-CD3 with CD8. Interacts with LCK.By similarity3 Publications

Protein-protein interaction databases

IntActiP10300, 3 interactors
MINTiP10300
STRINGi10090.ENSMUSP00000070131

Structurei

Secondary structure

1213
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi22 – 25Combined sources4
Beta strandi27 – 31Combined sources5
Beta strandi37 – 43Combined sources7
Beta strandi49 – 59Combined sources11
Beta strandi60 – 63Combined sources4
Beta strandi64 – 73Combined sources10
Turni74 – 76Combined sources3
Beta strandi77 – 80Combined sources4
Helixi82 – 84Combined sources3
Turni85 – 87Combined sources3
Beta strandi90 – 94Combined sources5
Beta strandi97 – 99Combined sources3
Beta strandi101 – 104Combined sources4
Helixi109 – 111Combined sources3
Beta strandi113 – 120Combined sources8
Beta strandi125 – 127Combined sources3
Beta strandi131 – 135Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ATPX-ray2.40B/D22-136[»]
3B9KX-ray2.70B/F22-138[»]
3DMMX-ray2.60D19-168[»]
ProteinModelPortaliP10300
SMRiP10300
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10300

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 133Ig-like V-typeAdd BLAST112

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IY5X Eukaryota
ENOG410ZF37 LUCA
GeneTreeiENSGT00510000048998
HOGENOMiHOG000008681
HOVERGENiHBG105700
InParanoidiP10300
KOiK06459
OMAiMRIYWLR
OrthoDBiEOG091G0T5W
PhylomeDBiP10300
TreeFamiTF338028

Family and domain databases

Gene3Di2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003599 Ig_sub
IPR013106 Ig_V-set
PfamiView protein in Pfam
PF07686 V-set, 1 hit
SMARTiView protein in SMART
SM00409 IG, 1 hit
SM00406 IGv, 1 hit
SUPFAMiSSF48726 SSF48726, 1 hit
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10300-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQPWLWLVFS MKLAALWSSS ALIQTPSSLL VQTNHTAKMS CEVKSISKLT
60 70 80 90 100
SIYWLRERQD PKDKYFEFLA SWSSSKGVLY GESVDKKRNI ILESSDSRRP
110 120 130 140 150
FLSIMNVKPE DSDFYFCATV GSPKMVFGTG TKLTVVDVLP TTAPTKKTTL
160 170 180 190 200
KMKKKKQCPF PHPETQKGLT CSLTTLSLLV VCILLLLAFL GVAVYFYCVR
210
RRARIHFMKQ FHK
Length:213
Mass (Da):24,288
Last modified:July 1, 1989 - v1
Checksum:i138D2DCA43215BDB
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti98R → S in allele Lyt-3A. 1
Natural varianti122 – 200Missing in allele Lyt-3-alpha. Add BLAST79

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07698 mRNA Translation: CAA30537.1
M19504 mRNA Translation: AAA39454.1
M26446
, M26441, M26442, M26443, M26444, M26445 Genomic DNA Translation: AAA39456.1
M16799 mRNA Translation: AAA39479.1
M17534 mRNA Translation: AAA39455.1
U34882 mRNA Translation: AAA92534.1
M22070
, M22065, M22066, M22067, M22068, M22069 Genomic DNA Translation: AAA39480.1
X07997 mRNA Translation: CAA30803.1
CCDSiCCDS39506.1
PIRiA30585
RefSeqiNP_033988.1, NM_009858.2
UniGeneiMm.333148

Genome annotation databases

EnsembliENSMUST00000065248; ENSMUSP00000070131; ENSMUSG00000053044
GeneIDi12526
KEGGimmu:12526
UCSCiuc009cgp.1 mouse

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCD8B_MOUSE
AccessioniPrimary (citable) accession number: P10300
Secondary accession number(s): Q31127, Q60966, Q61811
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: March 28, 2018
This is version 167 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health