Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

T-cell surface glycoprotein CD8 beta chain

Gene

Cd8b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiR-MMU-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD8 beta chain
Alternative name(s):
Lymphocyte antigen 3
T-cell membrane glycoprotein Ly-3
T-cell surface glycoprotein Lyt-3
CD_antigen: CD8b
Gene namesi
Name:Cd8b
Synonyms:Cd8b1, Ly-3, Lyt-3, Lyt3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:88347. Cd8b1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 175ExtracellularSequence analysisAdd BLAST154
Transmembranei176 – 196HelicalSequence analysisAdd BLAST21
Topological domaini197 – 213CytoplasmicSequence analysisAdd BLAST17

GO - Cellular componenti

  • cell surface Source: MGI
  • external side of plasma membrane Source: MGI
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000001464422 – 213T-cell surface glycoprotein CD8 beta chainAdd BLAST192

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi34N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi41 ↔ 117PROSITE-ProRule annotation2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP10300.
PaxDbiP10300.
PRIDEiP10300.

PTM databases

iPTMnetiP10300.
PhosphoSitePlusiP10300.
SwissPalmiP10300.

Expressioni

Gene expression databases

BgeeiENSMUSG00000053044.
CleanExiMM_CD8B1.
ExpressionAtlasiP10300. baseline and differential.
GenevisibleiP10300. MM.

Interactioni

Subunit structurei

In general heterodimer of an alpha and a beta chain linked by two disulfide bonds.2 Publications

Protein-protein interaction databases

IntActiP10300. 1 interactor.
MINTiMINT-8303335.
STRINGi10090.ENSMUSP00000070131.

Structurei

Secondary structure

1213
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi22 – 25Combined sources4
Beta strandi27 – 31Combined sources5
Beta strandi37 – 43Combined sources7
Beta strandi49 – 59Combined sources11
Beta strandi60 – 63Combined sources4
Beta strandi64 – 73Combined sources10
Turni74 – 76Combined sources3
Beta strandi77 – 80Combined sources4
Helixi82 – 84Combined sources3
Turni85 – 87Combined sources3
Beta strandi90 – 94Combined sources5
Beta strandi97 – 99Combined sources3
Beta strandi101 – 104Combined sources4
Helixi109 – 111Combined sources3
Beta strandi113 – 120Combined sources8
Beta strandi125 – 127Combined sources3
Beta strandi131 – 135Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ATPX-ray2.40B/D22-136[»]
3B9KX-ray2.70B/F22-138[»]
3DMMX-ray2.60D19-168[»]
ProteinModelPortaliP10300.
SMRiP10300.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10300.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 133Ig-like V-typeAdd BLAST112

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IY5X. Eukaryota.
ENOG410ZF37. LUCA.
GeneTreeiENSGT00510000048998.
HOGENOMiHOG000008681.
HOVERGENiHBG105700.
InParanoidiP10300.
KOiK06459.
OMAiIYWLRQR.
OrthoDBiEOG091G0T5W.
PhylomeDBiP10300.
TreeFamiTF338028.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00406. IGv. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P10300-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQPWLWLVFS MKLAALWSSS ALIQTPSSLL VQTNHTAKMS CEVKSISKLT
60 70 80 90 100
SIYWLRERQD PKDKYFEFLA SWSSSKGVLY GESVDKKRNI ILESSDSRRP
110 120 130 140 150
FLSIMNVKPE DSDFYFCATV GSPKMVFGTG TKLTVVDVLP TTAPTKKTTL
160 170 180 190 200
KMKKKKQCPF PHPETQKGLT CSLTTLSLLV VCILLLLAFL GVAVYFYCVR
210
RRARIHFMKQ FHK
Length:213
Mass (Da):24,288
Last modified:July 1, 1989 - v1
Checksum:i138D2DCA43215BDB
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti98R → S in allele Lyt-3A. 1
Natural varianti122 – 200Missing in allele Lyt-3-alpha. Add BLAST79

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07698 mRNA. Translation: CAA30537.1.
M19504 mRNA. Translation: AAA39454.1.
M26446
, M26441, M26442, M26443, M26444, M26445 Genomic DNA. Translation: AAA39456.1.
M16799 mRNA. Translation: AAA39479.1.
M17534 mRNA. Translation: AAA39455.1.
U34882 mRNA. Translation: AAA92534.1.
M22070
, M22065, M22066, M22067, M22068, M22069 Genomic DNA. Translation: AAA39480.1.
X07997 mRNA. Translation: CAA30803.1.
CCDSiCCDS39506.1.
PIRiA30585.
RefSeqiNP_033988.1. NM_009858.2.
UniGeneiMm.333148.

Genome annotation databases

EnsembliENSMUST00000065248; ENSMUSP00000070131; ENSMUSG00000053044.
GeneIDi12526.
KEGGimmu:12526.
UCSCiuc009cgp.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07698 mRNA. Translation: CAA30537.1.
M19504 mRNA. Translation: AAA39454.1.
M26446
, M26441, M26442, M26443, M26444, M26445 Genomic DNA. Translation: AAA39456.1.
M16799 mRNA. Translation: AAA39479.1.
M17534 mRNA. Translation: AAA39455.1.
U34882 mRNA. Translation: AAA92534.1.
M22070
, M22065, M22066, M22067, M22068, M22069 Genomic DNA. Translation: AAA39480.1.
X07997 mRNA. Translation: CAA30803.1.
CCDSiCCDS39506.1.
PIRiA30585.
RefSeqiNP_033988.1. NM_009858.2.
UniGeneiMm.333148.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ATPX-ray2.40B/D22-136[»]
3B9KX-ray2.70B/F22-138[»]
3DMMX-ray2.60D19-168[»]
ProteinModelPortaliP10300.
SMRiP10300.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP10300. 1 interactor.
MINTiMINT-8303335.
STRINGi10090.ENSMUSP00000070131.

PTM databases

iPTMnetiP10300.
PhosphoSitePlusiP10300.
SwissPalmiP10300.

Proteomic databases

EPDiP10300.
PaxDbiP10300.
PRIDEiP10300.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000065248; ENSMUSP00000070131; ENSMUSG00000053044.
GeneIDi12526.
KEGGimmu:12526.
UCSCiuc009cgp.1. mouse.

Organism-specific databases

CTDi12526.
MGIiMGI:88347. Cd8b1.

Phylogenomic databases

eggNOGiENOG410IY5X. Eukaryota.
ENOG410ZF37. LUCA.
GeneTreeiENSGT00510000048998.
HOGENOMiHOG000008681.
HOVERGENiHBG105700.
InParanoidiP10300.
KOiK06459.
OMAiIYWLRQR.
OrthoDBiEOG091G0T5W.
PhylomeDBiP10300.
TreeFamiTF338028.

Enzyme and pathway databases

ReactomeiR-MMU-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Miscellaneous databases

EvolutionaryTraceiP10300.
PROiP10300.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000053044.
CleanExiMM_CD8B1.
ExpressionAtlasiP10300. baseline and differential.
GenevisibleiP10300. MM.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00406. IGv. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCD8B_MOUSE
AccessioniPrimary (citable) accession number: P10300
Secondary accession number(s): Q31127, Q60966, Q61811
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.