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Protein

Glutathione S-transferase P

Gene

gst-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (By similarity). Prevents dopaminergic CEP neuron degeneration in response to Mn2+ (PubMed:23721876).By similarity1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei7GlutathioneBy similarity1
Binding sitei38GlutathioneBy similarity1
Binding sitei42GlutathioneBy similarity1

GO - Molecular functioni

  • glutathione transferase activity Source: WormBase

GO - Biological processi

  • defense response to Gram-negative bacterium Source: WormBase
  • innate immune response Source: WormBase
  • metabolic process Source: InterPro
  • negative regulation of cellular response to manganese ion Source: UniProtKB
  • negative regulation of neuron death Source: UniProtKB
  • positive regulation of cellular response to manganese ion Source: UniProtKB
  • positive regulation of neuron death Source: UniProtKB

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

ReactomeiR-CEL-156590. Glutathione conjugation.
R-CEL-3299685. Detoxification of Reactive Oxygen Species.
R-CEL-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase P (EC:2.5.1.18By similarity)
Alternative name(s):
GST class-pi
Gene namesi
Name:gst-1
ORF Names:R107.7
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiR107.7; CE00302; WBGene00001749; gst-1.

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown causes an increase in Mn2+-mediated dopaminergic CEP neuron degeneration.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001859111 – 208Glutathione S-transferase PAdd BLAST208

Proteomic databases

EPDiP10299.
PaxDbiP10299.
PeptideAtlasiP10299.
PRIDEiP10299.

Expressioni

Tissue specificityi

Expressed in dopaminergic (DA) neuron (at protein levels).1 Publication

Inductioni

By manganese.1 Publication

Gene expression databases

BgeeiWBGene00001749.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
gst-23P915055EBI-326030,EBI-326040

Protein-protein interaction databases

BioGridi41480. 1 interactor.
DIPiDIP-24298N.
IntActiP10299. 1 interactor.
MINTiMINT-1090976.
STRINGi6239.R107.7.2.

Structurei

3D structure databases

ProteinModelPortaliP10299.
SMRiP10299.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 78GST N-terminalAdd BLAST78
Domaini80 – 202GST C-terminalAdd BLAST123

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni49 – 50Glutathione bindingBy similarity2
Regioni62 – 63Glutathione bindingBy similarity2

Sequence similaritiesi

Belongs to the GST superfamily. Pi family.Curated

Phylogenomic databases

eggNOGiKOG1695. Eukaryota.
ENOG4111VAU. LUCA.
GeneTreeiENSGT00550000074559.
HOGENOMiHOG000115733.
InParanoidiP10299.
KOiK00799.
OMAiAYLNKRA.
OrthoDBiEOG091G0K2E.
PhylomeDBiP10299.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiView protein in InterPro
IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR003082. GST_pi.
IPR012336. Thioredoxin-like_fold.
PfamiView protein in Pfam
PF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
PRINTSiPR01268. GSTRNSFRASEP.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.

Sequencei

Sequence statusi: Complete.

P10299-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLKLTYFDI HGLAEPIRLL LADKQVAYED HRVTYEQWAD IKPKMIFGQV
60 70 80 90 100
PCLLSGDEEI VQSGAIIRHL ARLNGLNGSN ETETTFIDMF YEGLRDLHTK
110 120 130 140 150
YTTMIYRNYE DGKAPYIKDV LPGELARLEK LFHTYKNGEH YVIGDKESYA
160 170 180 190 200
DYVLFEELDI HLILTPNALD GVPALKKFHE RFAERPNIKA YLNKRAAINP

PVNGNGKQ
Length:208
Mass (Da):23,901
Last modified:July 1, 1989 - v1
Checksum:i3A0DC439FF8FFFBB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13689 mRNA. Translation: CAA31979.1.
Z14092 Genomic DNA. Translation: CAA78471.1.
PIRiS03615.
RefSeqiNP_499006.1. NM_066605.5.
UniGeneiCel.19723.

Genome annotation databases

EnsemblMetazoaiR107.7.1; R107.7.1; WBGene00001749.
R107.7.2; R107.7.2; WBGene00001749.
R107.7.3; R107.7.3; WBGene00001749.
GeneIDi176281.
KEGGicel:CELE_R107.7.
UCSCiR107.7.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13689 mRNA. Translation: CAA31979.1.
Z14092 Genomic DNA. Translation: CAA78471.1.
PIRiS03615.
RefSeqiNP_499006.1. NM_066605.5.
UniGeneiCel.19723.

3D structure databases

ProteinModelPortaliP10299.
SMRiP10299.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41480. 1 interactor.
DIPiDIP-24298N.
IntActiP10299. 1 interactor.
MINTiMINT-1090976.
STRINGi6239.R107.7.2.

Proteomic databases

EPDiP10299.
PaxDbiP10299.
PeptideAtlasiP10299.
PRIDEiP10299.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiR107.7.1; R107.7.1; WBGene00001749.
R107.7.2; R107.7.2; WBGene00001749.
R107.7.3; R107.7.3; WBGene00001749.
GeneIDi176281.
KEGGicel:CELE_R107.7.
UCSCiR107.7.1. c. elegans.

Organism-specific databases

CTDi176281.
WormBaseiR107.7; CE00302; WBGene00001749; gst-1.

Phylogenomic databases

eggNOGiKOG1695. Eukaryota.
ENOG4111VAU. LUCA.
GeneTreeiENSGT00550000074559.
HOGENOMiHOG000115733.
InParanoidiP10299.
KOiK00799.
OMAiAYLNKRA.
OrthoDBiEOG091G0K2E.
PhylomeDBiP10299.

Enzyme and pathway databases

ReactomeiR-CEL-156590. Glutathione conjugation.
R-CEL-3299685. Detoxification of Reactive Oxygen Species.
R-CEL-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiP10299.

Gene expression databases

BgeeiWBGene00001749.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiView protein in InterPro
IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR003082. GST_pi.
IPR012336. Thioredoxin-like_fold.
PfamiView protein in Pfam
PF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
PRINTSiPR01268. GSTRNSFRASEP.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGSTP1_CAEEL
AccessioniPrimary (citable) accession number: P10299
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: March 15, 2017
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.