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P10295 (ITR2_MOMCH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trypsin inhibitor 2
Alternative name(s):
MCTI-A
MCTI-II
Trypsin inhibitor II
OrganismMomordica charantia (Bitter gourd) (Balsam pear)
Taxonomic identifier3673 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeMomordiceaeMomordica

Protein attributes

Sequence length28 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits trypsin.

Subcellular location

Secreted.

Domain

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similarities

Belongs to the protease inhibitor I7 (squash-type serine protease inhibitor) family. [View classification]

Ontologies

Keywords
   Cellular componentSecreted
   DomainKnottin
   Molecular functionProtease inhibitor
Serine protease inhibitor
   PTMDisulfide bond
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type endopeptidase inhibitor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Peptide1 – 2828Trypsin inhibitor 2
PRO_0000044387

Sites

Site5 – 62Reactive bond

Amino acid modifications

Disulfide bond3 ↔ 20 Ref.1
Disulfide bond10 ↔ 22 Ref.1
Disulfide bond16 ↔ 27 Ref.1

Secondary structure

... 28
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P10295 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 67E263D36974FEDF

FASTA283,230
        10         20 
RICPRIWMEC KRDSDCMAQC ICVDGHCG 

« Hide

References

[1]"Amino acid sequences and disulfide bridges of serine proteinase inhibitors from bitter gourd (Momordica charantia LINN.) seeds."
Hara S., Makino J., Ikenaka T.
J. Biochem. 105:88-92(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, DISULFIDE BONDS.
Tissue: Seed.
[2]"Refined 1.6-A resolution crystal structure of the complex formed between porcine beta-trypsin and MCTI-A, a trypsin inhibitor of the squash family. Detailed comparison with bovine beta-trypsin and its complex."
Huang Q., Liu S., Tang Y.
J. Mol. Biol. 229:1022-1036(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
[3]"Amino acid sequencing of a trypsin inhibitor by refined 1.6 A X-ray crystal structure of its complex with porcine beta-trypsin."
Huang Q., Liu S., Tang Y., Zeng F., Qian R.
FEBS Lett. 297:143-146(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

PIRJC2507.
JX0058.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1F2SX-ray1.79I1-28[»]
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-108133.

Protein family/group databases

MEROPSI07.008.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000737. Prot_inh_squash.
IPR011052. Proteinase_amylase_inhib_dom.
[Graphical view]
PfamPF00299. Squash. 1 hit.
[Graphical view]
PRINTSPR00293. SQUASHINHBTR.
SUPFAMSSF57027. SSF57027. 1 hit.
PROSITEPS00286. SQUASH_INHIBITOR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP10295.

Entry information

Entry nameITR2_MOMCH
AccessionPrimary (citable) accession number: P10295
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references