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P10295

- ITR2_MOMCH

UniProt

P10295 - ITR2_MOMCH

Protein

Trypsin inhibitor 2

Gene
N/A
Organism
Momordica charantia (Bitter gourd) (Balsam pear)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Inhibits trypsin.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei5 – 62Reactive bond

    GO - Molecular functioni

    1. serine-type endopeptidase inhibitor activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Protease inhibitor, Serine protease inhibitor

    Protein family/group databases

    MEROPSiI07.008.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypsin inhibitor 2
    Alternative name(s):
    MCTI-A
    MCTI-II
    Trypsin inhibitor II
    OrganismiMomordica charantia (Bitter gourd) (Balsam pear)
    Taxonomic identifieri3673 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeMomordiceaeMomordica

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Peptidei1 – 2828Trypsin inhibitor 2PRO_0000044387Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi3 ↔ 201 Publication
    Disulfide bondi10 ↔ 221 Publication
    Disulfide bondi16 ↔ 271 Publication

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-108133.

    Structurei

    Secondary structure

    1
    28
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi13 – 153

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1F2SX-ray1.79I1-28[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP10295.

    Family & Domainsi

    Domaini

    The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

    Sequence similaritiesi

    Keywords - Domaini

    Knottin

    Family and domain databases

    InterProiIPR000737. Prot_inh_squash.
    IPR011052. Proteinase_amylase_inhib_dom.
    [Graphical view]
    PfamiPF00299. Squash. 1 hit.
    [Graphical view]
    PRINTSiPR00293. SQUASHINHBTR.
    SUPFAMiSSF57027. SSF57027. 1 hit.
    PROSITEiPS00286. SQUASH_INHIBITOR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P10295-1 [UniParc]FASTAAdd to Basket

    « Hide

    RICPRIWMEC KRDSDCMAQC ICVDGHCG                           28
    Length:28
    Mass (Da):3,230
    Last modified:July 1, 1989 - v1
    Checksum:i67E263D36974FEDF
    GO

    Sequence databases

    PIRiJC2507.
    JX0058.

    Cross-referencesi

    Sequence databases

    PIRi JC2507.
    JX0058.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1F2S X-ray 1.79 I 1-28 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-108133.

    Protein family/group databases

    MEROPSi I07.008.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P10295.

    Family and domain databases

    InterProi IPR000737. Prot_inh_squash.
    IPR011052. Proteinase_amylase_inhib_dom.
    [Graphical view ]
    Pfami PF00299. Squash. 1 hit.
    [Graphical view ]
    PRINTSi PR00293. SQUASHINHBTR.
    SUPFAMi SSF57027. SSF57027. 1 hit.
    PROSITEi PS00286. SQUASH_INHIBITOR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Amino acid sequences and disulfide bridges of serine proteinase inhibitors from bitter gourd (Momordica charantia LINN.) seeds."
      Hara S., Makino J., Ikenaka T.
      J. Biochem. 105:88-92(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, DISULFIDE BONDS.
      Tissue: Seed.
    2. "Refined 1.6-A resolution crystal structure of the complex formed between porcine beta-trypsin and MCTI-A, a trypsin inhibitor of the squash family. Detailed comparison with bovine beta-trypsin and its complex."
      Huang Q., Liu S., Tang Y.
      J. Mol. Biol. 229:1022-1036(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
    3. "Amino acid sequencing of a trypsin inhibitor by refined 1.6 A X-ray crystal structure of its complex with porcine beta-trypsin."
      Huang Q., Liu S., Tang Y., Zeng F., Qian R.
      FEBS Lett. 297:143-146(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

    Entry informationi

    Entry nameiITR2_MOMCH
    AccessioniPrimary (citable) accession number: P10295
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3