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P10295

- ITR2_MOMCH

UniProt

P10295 - ITR2_MOMCH

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Protein

Trypsin inhibitor 2

Gene
N/A
Organism
Momordica charantia (Bitter gourd) (Balsam pear)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Inhibits trypsin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei5 – 62Reactive bond

GO - Molecular functioni

  1. serine-type endopeptidase inhibitor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI07.008.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin inhibitor 2
Alternative name(s):
MCTI-A
MCTI-II
Trypsin inhibitor II
OrganismiMomordica charantia (Bitter gourd) (Balsam pear)
Taxonomic identifieri3673 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeMomordiceaeMomordica

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei1 – 2828Trypsin inhibitor 2PRO_0000044387Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi3 ↔ 201 Publication
Disulfide bondi10 ↔ 221 Publication
Disulfide bondi16 ↔ 271 Publication

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

MINTiMINT-108133.

Structurei

Secondary structure

1
28
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 153Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F2SX-ray1.79I1-28[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP10295.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Keywords - Domaini

Knottin

Family and domain databases

InterProiIPR000737. Prot_inh_squash.
IPR011052. Proteinase_amylase_inhib_dom.
[Graphical view]
PfamiPF00299. Squash. 1 hit.
[Graphical view]
PRINTSiPR00293. SQUASHINHBTR.
SUPFAMiSSF57027. SSF57027. 1 hit.
PROSITEiPS00286. SQUASH_INHIBITOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P10295-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20 
RICPRIWMEC KRDSDCMAQC ICVDGHCG
Length:28
Mass (Da):3,230
Last modified:July 1, 1989 - v1
Checksum:i67E263D36974FEDF
GO

Sequence databases

PIRiJC2507.
JX0058.

Cross-referencesi

Sequence databases

PIRi JC2507.
JX0058.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1F2S X-ray 1.79 I 1-28 [» ]
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-108133.

Protein family/group databases

MEROPSi I07.008.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P10295.

Family and domain databases

InterProi IPR000737. Prot_inh_squash.
IPR011052. Proteinase_amylase_inhib_dom.
[Graphical view ]
Pfami PF00299. Squash. 1 hit.
[Graphical view ]
PRINTSi PR00293. SQUASHINHBTR.
SUPFAMi SSF57027. SSF57027. 1 hit.
PROSITEi PS00286. SQUASH_INHIBITOR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Amino acid sequences and disulfide bridges of serine proteinase inhibitors from bitter gourd (Momordica charantia LINN.) seeds."
    Hara S., Makino J., Ikenaka T.
    J. Biochem. 105:88-92(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, DISULFIDE BONDS.
    Tissue: Seed.
  2. "Refined 1.6-A resolution crystal structure of the complex formed between porcine beta-trypsin and MCTI-A, a trypsin inhibitor of the squash family. Detailed comparison with bovine beta-trypsin and its complex."
    Huang Q., Liu S., Tang Y.
    J. Mol. Biol. 229:1022-1036(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
  3. "Amino acid sequencing of a trypsin inhibitor by refined 1.6 A X-ray crystal structure of its complex with porcine beta-trypsin."
    Huang Q., Liu S., Tang Y., Zeng F., Qian R.
    FEBS Lett. 297:143-146(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

Entry informationi

Entry nameiITR2_MOMCH
AccessioniPrimary (citable) accession number: P10295
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 26, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3